CA3133986A1 - Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health - Google Patents

Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health Download PDF

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Publication number
CA3133986A1
CA3133986A1 CA3133986A CA3133986A CA3133986A1 CA 3133986 A1 CA3133986 A1 CA 3133986A1 CA 3133986 A CA3133986 A CA 3133986A CA 3133986 A CA3133986 A CA 3133986A CA 3133986 A1 CA3133986 A1 CA 3133986A1
Authority
CA
Canada
Prior art keywords
seq
amino acids
targeting sequence
exosporium
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3133986A
Other languages
English (en)
French (fr)
Inventor
Damian CURTIS
Kevin Bugg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience LP
Original Assignee
Bayer CropScience LP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer CropScience LP filed Critical Bayer CropScience LP
Publication of CA3133986A1 publication Critical patent/CA3133986A1/en
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3133986A 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health Pending CA3133986A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962820773P 2019-03-19 2019-03-19
US62/820,773 2019-03-19
PCT/US2020/023255 WO2020190993A1 (en) 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health

Publications (1)

Publication Number Publication Date
CA3133986A1 true CA3133986A1 (en) 2020-09-24

Family

ID=70293065

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3133986A Pending CA3133986A1 (en) 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health

Country Status (12)

Country Link
US (2) US11793196B2 (enExample)
EP (1) EP3941204A1 (enExample)
JP (1) JP2022525639A (enExample)
KR (1) KR20210142673A (enExample)
CN (1) CN113825401A (enExample)
AR (1) AR118443A1 (enExample)
AU (1) AU2020241392B2 (enExample)
BR (1) BR112021017827A2 (enExample)
CA (1) CA3133986A1 (enExample)
CR (1) CR20210478A (enExample)
MX (1) MX2021011358A (enExample)
WO (1) WO2020190993A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR113123A1 (es) 2017-09-20 2020-01-29 Spogen Biotech Inc Proteínas de fusión, bacterias recombinantes y fragmentos del exosporio para promover la salud de las plantas
AR118443A1 (es) * 2019-03-19 2021-10-06 Bayer Cropscience Lp Proteínas de fusión, bacterias recombinantes y fragmentos de exosporas para control de plagas y salud de las plantas
BR112023004843A2 (pt) * 2020-09-21 2023-04-18 Bayer Cropscience Lp Serina proteases
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
WO2023092050A1 (en) * 2021-11-20 2023-05-25 Bayer Cropscience Lp Beneficial combinations with recombinant bacillus cells expressing a serine protease

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9518323D0 (en) * 1995-09-07 1995-11-08 Steidler Lothar Materials and methods relating to the attachment and display of substances on cell surfaces
US9133251B2 (en) * 2008-02-22 2015-09-15 The Curators Of The University Of Missouri Bacillus based delivery system and methods of use
US20100112670A1 (en) * 2008-06-25 2010-05-06 Vaxiion Therapeutics, Inc. Regulated genetic suicide mechanism compositions and methods
EP2331536B1 (en) * 2008-08-22 2013-08-21 Syngenta Participations AG Insecticidal compounds
EP2651220A1 (en) * 2010-12-15 2013-10-23 Syngenta Participations AG Pesticidal mixtures
DE102012201297A1 (de) 2012-01-31 2013-08-01 Basf Se Expressionsverfahren
KR102047336B1 (ko) 2012-02-01 2019-11-22 다우 아그로사이언시즈 엘엘씨 신규한 클래스의 글리포세이트 저항성 유전자
US9573980B2 (en) 2013-03-15 2017-02-21 Spogen Biotech Inc. Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing Bacillus spores on plant roots
AR101957A1 (es) * 2014-09-17 2017-01-25 Bayer Cropscience Lp Composiciones que comprenden células recombinantes de bacillus y un insecticida
WO2016044563A1 (en) 2014-09-17 2016-03-24 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and a fungicide
IL315468A (en) 2014-09-17 2024-11-01 Spogen Biotech Inc Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
MX390380B (es) 2014-09-17 2025-03-20 Basf Corp Composiciones que comprenden celulas recombinantes de bacillus y otro agente de control biologico.
AR101961A1 (es) 2014-09-17 2017-01-25 Bayer Cropscience Lp Composiciones que comprenden células recombinantes de bacillus y otro agente de control biológico
WO2016044575A1 (en) 2014-09-17 2016-03-24 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and an insecticide
RU2017113002A (ru) * 2014-09-17 2018-10-17 Байер Кропсайенс Лп Композиции, содержащие рекомбинантные клетки bacillus и фунгицид
JP6581346B2 (ja) * 2014-11-19 2019-09-25 花王株式会社 精製アルカリプロテアーゼの製造方法
CA3016922A1 (en) 2016-03-16 2017-09-21 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and exosporium fragments for animal health and aquaculture
AR113123A1 (es) 2017-09-20 2020-01-29 Spogen Biotech Inc Proteínas de fusión, bacterias recombinantes y fragmentos del exosporio para promover la salud de las plantas
AR118443A1 (es) * 2019-03-19 2021-10-06 Bayer Cropscience Lp Proteínas de fusión, bacterias recombinantes y fragmentos de exosporas para control de plagas y salud de las plantas

Also Published As

Publication number Publication date
CR20210478A (es) 2021-11-05
AU2020241392B2 (en) 2025-05-01
JP2022525639A (ja) 2022-05-18
US20240008485A1 (en) 2024-01-11
KR20210142673A (ko) 2021-11-25
US12527323B2 (en) 2026-01-20
BR112021017827A2 (pt) 2021-11-30
CN113825401A (zh) 2021-12-21
AU2020241392A1 (en) 2021-09-30
MX2021011358A (es) 2021-10-13
US11793196B2 (en) 2023-10-24
US20200296960A1 (en) 2020-09-24
WO2020190993A1 (en) 2020-09-24
EP3941204A1 (en) 2022-01-26
AR118443A1 (es) 2021-10-06

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