AU2020241392B2 - Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health - Google Patents

Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health Download PDF

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Publication number
AU2020241392B2
AU2020241392B2 AU2020241392A AU2020241392A AU2020241392B2 AU 2020241392 B2 AU2020241392 B2 AU 2020241392B2 AU 2020241392 A AU2020241392 A AU 2020241392A AU 2020241392 A AU2020241392 A AU 2020241392A AU 2020241392 B2 AU2020241392 B2 AU 2020241392B2
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AU
Australia
Prior art keywords
seq
amino acids
exosporium
targeting sequence
protein
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Expired - Fee Related
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AU2020241392A
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English (en)
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AU2020241392A1 (en
Inventor
Kevin Bugg
Damian CURTIS
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Bayer CropScience LP
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Bayer CropScience LP
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Publication of AU2020241392A1 publication Critical patent/AU2020241392A1/en
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Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2020241392A 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health Expired - Fee Related AU2020241392B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962820773P 2019-03-19 2019-03-19
US62/820,773 2019-03-19
PCT/US2020/023255 WO2020190993A1 (en) 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health

Publications (2)

Publication Number Publication Date
AU2020241392A1 AU2020241392A1 (en) 2021-09-30
AU2020241392B2 true AU2020241392B2 (en) 2025-05-01

Family

ID=70293065

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020241392A Expired - Fee Related AU2020241392B2 (en) 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health

Country Status (12)

Country Link
US (2) US11793196B2 (enExample)
EP (1) EP3941204A1 (enExample)
JP (1) JP2022525639A (enExample)
KR (1) KR20210142673A (enExample)
CN (1) CN113825401A (enExample)
AR (1) AR118443A1 (enExample)
AU (1) AU2020241392B2 (enExample)
BR (1) BR112021017827A2 (enExample)
CA (1) CA3133986A1 (enExample)
CR (1) CR20210478A (enExample)
MX (1) MX2021011358A (enExample)
WO (1) WO2020190993A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR113123A1 (es) 2017-09-20 2020-01-29 Spogen Biotech Inc Proteínas de fusión, bacterias recombinantes y fragmentos del exosporio para promover la salud de las plantas
AR118443A1 (es) * 2019-03-19 2021-10-06 Bayer Cropscience Lp Proteínas de fusión, bacterias recombinantes y fragmentos de exosporas para control de plagas y salud de las plantas
BR112023004843A2 (pt) * 2020-09-21 2023-04-18 Bayer Cropscience Lp Serina proteases
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
WO2023092050A1 (en) * 2021-11-20 2023-05-25 Bayer Cropscience Lp Beneficial combinations with recombinant bacillus cells expressing a serine protease

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016044661A1 (en) * 2014-09-17 2016-03-24 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria

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GB9518323D0 (en) * 1995-09-07 1995-11-08 Steidler Lothar Materials and methods relating to the attachment and display of substances on cell surfaces
US9133251B2 (en) * 2008-02-22 2015-09-15 The Curators Of The University Of Missouri Bacillus based delivery system and methods of use
US20100112670A1 (en) * 2008-06-25 2010-05-06 Vaxiion Therapeutics, Inc. Regulated genetic suicide mechanism compositions and methods
EP2331536B1 (en) * 2008-08-22 2013-08-21 Syngenta Participations AG Insecticidal compounds
EP2651220A1 (en) * 2010-12-15 2013-10-23 Syngenta Participations AG Pesticidal mixtures
DE102012201297A1 (de) 2012-01-31 2013-08-01 Basf Se Expressionsverfahren
KR102047336B1 (ko) 2012-02-01 2019-11-22 다우 아그로사이언시즈 엘엘씨 신규한 클래스의 글리포세이트 저항성 유전자
US9573980B2 (en) 2013-03-15 2017-02-21 Spogen Biotech Inc. Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing Bacillus spores on plant roots
AR101957A1 (es) * 2014-09-17 2017-01-25 Bayer Cropscience Lp Composiciones que comprenden células recombinantes de bacillus y un insecticida
WO2016044563A1 (en) 2014-09-17 2016-03-24 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and a fungicide
MX390380B (es) 2014-09-17 2025-03-20 Basf Corp Composiciones que comprenden celulas recombinantes de bacillus y otro agente de control biologico.
AR101961A1 (es) 2014-09-17 2017-01-25 Bayer Cropscience Lp Composiciones que comprenden células recombinantes de bacillus y otro agente de control biológico
WO2016044575A1 (en) 2014-09-17 2016-03-24 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and an insecticide
RU2017113002A (ru) * 2014-09-17 2018-10-17 Байер Кропсайенс Лп Композиции, содержащие рекомбинантные клетки bacillus и фунгицид
JP6581346B2 (ja) * 2014-11-19 2019-09-25 花王株式会社 精製アルカリプロテアーゼの製造方法
CA3016922A1 (en) 2016-03-16 2017-09-21 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and exosporium fragments for animal health and aquaculture
AR113123A1 (es) 2017-09-20 2020-01-29 Spogen Biotech Inc Proteínas de fusión, bacterias recombinantes y fragmentos del exosporio para promover la salud de las plantas
AR118443A1 (es) * 2019-03-19 2021-10-06 Bayer Cropscience Lp Proteínas de fusión, bacterias recombinantes y fragmentos de exosporas para control de plagas y salud de las plantas

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WO2016044661A1 (en) * 2014-09-17 2016-03-24 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria

Non-Patent Citations (2)

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Title
CE GENG ET AL: "A novel serine protease, Sep1, from Bacillus firmus DS-1 has nematicidal activity and degrades multiple intestinal-associated nematode proteins", SCIENTIFIC REPORTS, vol. 6, no. 1, 27 April 2016. *
DATABASE UniProt [online] 16 April 2014 (2014-04-16), "SubName: Full=Intracellular serine protease {ECO:0000313|EMBL:EWG10090.1};", XP055707253, retrieved from EBI accession no. UNIPROT:W7KRH1 Database accession no. W7KRH1 *

Also Published As

Publication number Publication date
CR20210478A (es) 2021-11-05
CA3133986A1 (en) 2020-09-24
JP2022525639A (ja) 2022-05-18
US20240008485A1 (en) 2024-01-11
KR20210142673A (ko) 2021-11-25
US12527323B2 (en) 2026-01-20
BR112021017827A2 (pt) 2021-11-30
CN113825401A (zh) 2021-12-21
AU2020241392A1 (en) 2021-09-30
MX2021011358A (es) 2021-10-13
US11793196B2 (en) 2023-10-24
US20200296960A1 (en) 2020-09-24
WO2020190993A1 (en) 2020-09-24
EP3941204A1 (en) 2022-01-26
AR118443A1 (es) 2021-10-06

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