CA3110254C - Afucosylated antibodies and manufacture thereof - Google Patents

Afucosylated antibodies and manufacture thereof Download PDF

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Publication number
CA3110254C
CA3110254C CA3110254A CA3110254A CA3110254C CA 3110254 C CA3110254 C CA 3110254C CA 3110254 A CA3110254 A CA 3110254A CA 3110254 A CA3110254 A CA 3110254A CA 3110254 C CA3110254 C CA 3110254C
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CA
Canada
Prior art keywords
antibody
afucosylated
cells
cell
host cell
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3110254A
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English (en)
French (fr)
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CA3110254A1 (en
Inventor
Wen-Jiun Peng
Hui-Jung Chen
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United Biopharma Inc
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United Biopharma Inc
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Publication of CA3110254A1 publication Critical patent/CA3110254A1/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01068—Glycoprotein 6-alpha-L-fucosyltransferase (2.4.1.68), i.e. FUT8
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01047—GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/734—Complement-dependent cytotoxicity [CDC]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3110254A 2018-08-29 2018-08-29 Afucosylated antibodies and manufacture thereof Active CA3110254C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/102995 WO2020042015A1 (en) 2018-08-29 2018-08-29 Afucosylated antibodies and manufacture thereof

Publications (2)

Publication Number Publication Date
CA3110254A1 CA3110254A1 (en) 2020-03-05
CA3110254C true CA3110254C (en) 2023-08-29

Family

ID=69642839

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3110254A Active CA3110254C (en) 2018-08-29 2018-08-29 Afucosylated antibodies and manufacture thereof

Country Status (11)

Country Link
US (1) US20210317499A1 (US07655688-20100202-C00086.png)
EP (1) EP3818149A4 (US07655688-20100202-C00086.png)
JP (1) JP7216806B2 (US07655688-20100202-C00086.png)
KR (1) KR102596303B1 (US07655688-20100202-C00086.png)
CN (1) CN113166728A (US07655688-20100202-C00086.png)
AU (1) AU2018438767B9 (US07655688-20100202-C00086.png)
BR (1) BR112021003765A2 (US07655688-20100202-C00086.png)
CA (1) CA3110254C (US07655688-20100202-C00086.png)
MY (1) MY197429A (US07655688-20100202-C00086.png)
SG (1) SG11202101099PA (US07655688-20100202-C00086.png)
WO (1) WO2020042015A1 (US07655688-20100202-C00086.png)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113166765B (zh) * 2018-08-29 2024-03-26 联合生物制药股份有限公司 去岩藻醣基化抗体及其制造
TW202317614A (zh) 2021-06-07 2023-05-01 美商安進公司 使用岩藻糖苷酶控制糖基化蛋白的去岩藻糖基化水平
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA013563B1 (ru) 2000-10-06 2010-06-30 Киова Хакко Кирин Ко., Лтд. Трансгенное животное, продуцирующее антитела с измененными углеводными цепями, способ получения антител и содержащее антитела лекарственное средство
EP1498485A4 (en) * 2002-04-09 2006-09-06 Kyowa Hakko Kogyo Kk CELLS WITH MODIFIED GENOM
CA2608818A1 (en) * 2005-06-03 2006-12-14 Genentech, Inc. Method of producing antibodies with modified fucosylation level
WO2008090958A1 (ja) 2007-01-24 2008-07-31 Kyowa Hakko Kirin Co., Ltd. ドメイン交換された遺伝子組換え抗体組成物
AU2010256753B2 (en) 2009-06-02 2013-11-28 Regeneron Pharmaceuticals, Inc. Fucosylation-deficient cells
US8469819B2 (en) * 2009-06-04 2013-06-25 Michael Parker McMain Game apparatus and game control method for controlling and representing magical ability and power of a player character in an action power control program
WO2011108502A1 (ja) 2010-03-02 2011-09-09 協和発酵キリン株式会社 改変抗体組成物
JP2014509850A (ja) 2011-03-06 2014-04-24 メルク セロノ ソシエテ アノニム 低フコース細胞株およびその使用
IL217216A0 (en) * 2011-12-27 2012-07-31 Merck Serono Sa Low fucose cell lins and uses thereof
WO2016016842A1 (en) * 2014-07-30 2016-02-04 Zumutor Biologics, Inc. Non-fucosylated protein and methods thereof
EP3925970A1 (en) * 2015-11-02 2021-12-22 F. Hoffmann-La Roche AG Methods of making fucosylated and afucosylated forms of a protein
CN106167525B (zh) * 2016-04-01 2019-03-19 北京康明百奥新药研发有限公司 筛选超低岩藻糖细胞系的方法和应用
CN106701823A (zh) * 2017-01-18 2017-05-24 上海交通大学 生产无岩藻糖单克隆抗体的cho细胞系建立及其应用
CN107881160A (zh) * 2017-08-11 2018-04-06 百奥泰生物科技(广州)有限公司 一种由基因组被编辑的cho宿主细胞产生的具有独特糖谱的重组抗体及其制备方法

Also Published As

Publication number Publication date
BR112021003765A2 (pt) 2021-05-25
JP2021534801A (ja) 2021-12-16
AU2018438767B9 (en) 2023-10-05
AU2018438767B1 (US07655688-20100202-C00086.png) 2023-07-13
EP3818149A4 (en) 2022-03-23
WO2020042015A1 (en) 2020-03-05
EP3818149A1 (en) 2021-05-12
JP7216806B2 (ja) 2023-02-01
KR102596303B1 (ko) 2023-10-30
CA3110254A1 (en) 2020-03-05
AU2018438767A1 (en) 2021-04-01
US20210317499A1 (en) 2021-10-14
KR20210047316A (ko) 2021-04-29
SG11202101099PA (en) 2021-03-30
CN113166728A (zh) 2021-07-23
MY197429A (en) 2023-06-16

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