CA3101968A1 - Apparatus and method for absolute quantification of biomarkers for solid tumor diagnosis - Google Patents
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762526425P | 2017-06-29 | 2017-06-29 | |
| US62/526,425 | 2017-06-29 | ||
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| US20160250220A1 (en) | 2013-10-18 | 2016-09-01 | University Health Network | Treatment for pancreatic cancer |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
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| SG11201808629UA (en) * | 2016-04-21 | 2018-11-29 | Immatics Biotechnologies Gmbh | Immunotherapy against melanoma and other cancers |
| DE102016209871A1 (de) | 2016-06-06 | 2017-12-07 | Robert Bosch Gmbh | Stanzvorrichtung und Verfahren zum Stanzen eines Lumens und Implantieren einer Implantateinrichtung |
| DE102018201030B4 (de) * | 2018-01-24 | 2025-10-16 | Kardion Gmbh | Magnetkuppelelement mit magnetischer Lagerungsfunktion |
| DE102018206750A1 (de) | 2018-05-02 | 2019-11-07 | Kardion Gmbh | Vorrichtung zur induktiven Energieübertragung in einen menschlichen Körper und deren Verwendung |
| DE102018206754A1 (de) | 2018-05-02 | 2019-11-07 | Kardion Gmbh | Verfahren und Vorrichtung zur Bestimmung der Temperatur an einer Oberfläche sowie Verwendung des Verfahrens |
| DE102018206725A1 (de) | 2018-05-02 | 2019-11-07 | Kardion Gmbh | Empfangseinheit, Sendeeinheit, Energieübertragungssystem und Verfahren zur drahtlosen Energieübertragung |
| DE102018206727A1 (de) | 2018-05-02 | 2019-11-07 | Kardion Gmbh | Energieübertragungssystem und Empfangseinheit zur drahtlosen transkutanen Energieübertragung |
| DE102018206724A1 (de) | 2018-05-02 | 2019-11-07 | Kardion Gmbh | Energieübertragungssystem und Verfahren zur drahtlosen Energieübertragung |
| DE102018206731A1 (de) | 2018-05-02 | 2019-11-07 | Kardion Gmbh | Vorrichtung zur induktiven Energieübertragung in einen menschlichen Körper und Verwendung der Vorrichtung |
| DE102018207611A1 (de) | 2018-05-16 | 2019-11-21 | Kardion Gmbh | Rotorlagerungssystem |
| DE102018207575A1 (de) | 2018-05-16 | 2019-11-21 | Kardion Gmbh | Magnetische Stirndreh-Kupplung zur Übertragung von Drehmomenten |
| DE102018208539A1 (de) | 2018-05-30 | 2019-12-05 | Kardion Gmbh | Motorgehäusemodul zum Abdichten eines Motorraums eines Motors eines Herzunterstützungssystems und Herzunterstützungssystem und Verfahren zum Montieren eines Herzunterstützungssystems |
| DE102018208538A1 (de) | 2018-05-30 | 2019-12-05 | Kardion Gmbh | Intravasale Blutpumpe und Verfahren zur Herstellung von elektrischen Leiterbahnen |
| DE102018208541A1 (de) | 2018-05-30 | 2019-12-05 | Kardion Gmbh | Axialpumpe für ein Herzunterstützungssystem und Verfahren zum Herstellen einer Axialpumpe für ein Herzunterstützungssystem |
| DE102018208549A1 (de) | 2018-05-30 | 2019-12-05 | Kardion Gmbh | Elektronikmodul für ein Herzunterstützungssystem und Verfahren zum Herstellen eines Elektronikmoduls für ein Herzunterstützungssystem |
| DE102018208550A1 (de) | 2018-05-30 | 2019-12-05 | Kardion Gmbh | Leitungsvorrichtung zum Leiten eines Blutstroms für ein Herzunterstützungssystem, Herzunterstützungssystem und Verfahren zum Herstellen einer Leitungsvorrichtung |
| DE102018208555A1 (de) | 2018-05-30 | 2019-12-05 | Kardion Gmbh | Vorrichtung zum Verankern eines Herzunterstützungssystems in einem Blutgefäß, Verfahren zum Betreiben und Herstellverfahren zum Herstellen einer Vorrichtung und Herzunterstützungssystem |
| DE102018210076A1 (de) | 2018-06-21 | 2019-12-24 | Kardion Gmbh | Verfahren und Vorrichtung zum Erkennen eines Verschleißzustands eines Herzunterstützungssystems, Verfahren und Vorrichtung zum Betreiben eines Herzunterstützungssystems und Herzunterstützungssystem |
| DE102018210058A1 (de) | 2018-06-21 | 2019-12-24 | Kardion Gmbh | Statorschaufelvorrichtung zur Strömungsführung eines aus einer Austrittsöffnung eines Herzunterstützungssystems ausströmenden Fluids, Herzunterstützungssystem mit Statorschaufelvorrichtung, Verfahren zum Betreiben einer Statorschaufelvorrichtung und Herstellverfahren |
| EP3818496A4 (en) * | 2018-07-02 | 2022-04-20 | Tempus Labs, Inc. | 3D RADIOMICS PLATFORM FOR IMAGING BIOMARKER DEVELOPMENT |
| DE102018211297A1 (de) | 2018-07-09 | 2020-01-09 | Kardion Gmbh | Herzunterstützungssystem und Verfahren zur Überwachung der Integrität einer Haltestruktur eines Herzunterstützungssystems |
| DE102018211327A1 (de) | 2018-07-10 | 2020-01-16 | Kardion Gmbh | Laufrad für ein implantierbares, vaskuläres Unterstützungssystem |
| DE102018211328A1 (de) | 2018-07-10 | 2020-01-16 | Kardion Gmbh | Laufradgehäuse für ein implantierbares, vaskuläres Unterstützungssystem |
| DE102018212153A1 (de) | 2018-07-20 | 2020-01-23 | Kardion Gmbh | Zulaufleitung für eine Pumpeneinheit eines Herzunterstützungssystems, Herzunterstützungssystem und Verfahren zum Herstellen einer Zulaufleitung für eine Pumpeneinheit eines Herzunterstützungssystems |
| AU2019320533B2 (en) | 2018-08-07 | 2024-11-21 | Kardion Gmbh | Bearing device for a cardiac support system, and method for flushing an intermediate space in a bearing device for a cardiac support system |
| CN114631027A (zh) * | 2019-11-01 | 2022-06-14 | 烟台康体诊医疗科技有限公司 | 基于定量生物标志物的癌症诊断方法及其数据库 |
| AU2021207312A1 (en) * | 2020-01-17 | 2022-06-30 | Universität Zürich | Single cell pathology analysis of tumour samples |
| DE102020102474A1 (de) | 2020-01-31 | 2021-08-05 | Kardion Gmbh | Pumpe zum Fördern eines Fluids und Verfahren zum Herstellen einer Pumpe |
| US11699551B2 (en) | 2020-11-05 | 2023-07-11 | Kardion Gmbh | Device for inductive energy transmission in a human body and use of the device |
Family Cites Families (15)
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| AU2003252235A1 (en) * | 2002-07-25 | 2004-02-16 | Ajinomoto Co., Inc. | Method of analyzing protein |
| DE60200248T2 (de) * | 2002-08-01 | 2005-01-27 | Mtm Laboratories Ag | Verfahren für Lösung-basierte Diagnose |
| DE102005023011A1 (de) * | 2005-05-19 | 2006-11-23 | Qiagen Gmbh | Quantitative Proteinbestimmung von Formalin fixiertem Gewebe |
| CA2653398A1 (en) * | 2006-05-26 | 2007-12-06 | Board Of Regents, The University Of Texas System | Reverse phase protein array, protein activation and expression signatures, and associated methods |
| GB0611669D0 (en) * | 2006-06-13 | 2006-07-19 | Astrazeneca Uk Ltd | Mass spectrometry biomarker assay |
| JPWO2008075664A1 (ja) * | 2006-12-21 | 2010-04-15 | 味の素株式会社 | 癌の評価方法、ならびに癌評価装置、癌評価方法、癌評価システム、癌評価プログラムおよび記録媒体 |
| US8771962B2 (en) * | 2008-10-02 | 2014-07-08 | Sarka O. Southern | Health test for a broad spectrum of health problems |
| EP2239675A1 (en) | 2009-04-07 | 2010-10-13 | BIOCRATES Life Sciences AG | Method for in vitro diagnosing a complex disease |
| JP2013099253A (ja) * | 2010-03-11 | 2013-05-23 | Intec Systems Institute Inc | 肺癌または子宮頸癌の診断マーカー |
| PT2714926E (pt) * | 2011-05-25 | 2015-11-03 | Novartis Ag | Biomarcadores para o cancro do pulmão |
| US8293487B1 (en) * | 2012-01-06 | 2012-10-23 | Jiandi Zhang | Zestern, a simple, fast, specific and quantifiable improvement of immunodetection process |
| WO2016172216A1 (en) * | 2015-04-20 | 2016-10-27 | The Methodist Hospital | Use of soluble crkl compositions as breast cancer biomarkers and predictors of cancer metastasis |
| US9933418B2 (en) * | 2015-05-26 | 2018-04-03 | Quanticision Diagnostics Inc. | Multi-unit plate for electroblotting and immunoblot analysis |
| IL294138A (en) * | 2015-05-29 | 2022-08-01 | Genentech Inc | Therapeutic and diagnostic methods for cancer |
| EP3465200A4 (en) * | 2016-06-05 | 2020-07-08 | Berg LLC | PATIENT STRATIFICATION SYSTEMS AND METHODS AND IDENTIFICATION OF POTENTIAL BIOMARKERS |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160250220A1 (en) | 2013-10-18 | 2016-09-01 | University Health Network | Treatment for pancreatic cancer |
| US9642856B2 (en) | 2013-10-18 | 2017-05-09 | University Health Network | Treatment for pancreatic cancer |
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| US10788484B2 (en) | 2020-09-29 |
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