CA3059288C - Novel colorimetric sensing method for dual pathogen - Google Patents

Novel colorimetric sensing method for dual pathogen Download PDF

Info

Publication number
CA3059288C
CA3059288C CA3059288A CA3059288A CA3059288C CA 3059288 C CA3059288 C CA 3059288C CA 3059288 A CA3059288 A CA 3059288A CA 3059288 A CA3059288 A CA 3059288A CA 3059288 C CA3059288 C CA 3059288C
Authority
CA
Canada
Prior art keywords
seq
set forth
staphylococcus aureus
nucleotide sequence
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3059288A
Other languages
English (en)
French (fr)
Other versions
CA3059288A1 (en
Inventor
Yunbo LUO
Wentao Xu
Kunlun HUANG
Jingjing TIAN
Zaihui DU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Agricultural University
Original Assignee
China Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Agricultural University filed Critical China Agricultural University
Publication of CA3059288A1 publication Critical patent/CA3059288A1/en
Application granted granted Critical
Publication of CA3059288C publication Critical patent/CA3059288C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3059288A 2018-02-08 2018-08-08 Novel colorimetric sensing method for dual pathogen Active CA3059288C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201810128946.4A CN108251514A (zh) 2018-02-08 2018-02-08 一种双重致病菌的比色传感新方法
CN201810128946.4 2018-02-08
PCT/CN2018/099324 WO2019153675A1 (zh) 2018-02-08 2018-08-08 一种双重致病菌的比色传感新方法

Publications (2)

Publication Number Publication Date
CA3059288A1 CA3059288A1 (en) 2019-08-15
CA3059288C true CA3059288C (en) 2022-03-22

Family

ID=62744310

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3059288A Active CA3059288C (en) 2018-02-08 2018-08-08 Novel colorimetric sensing method for dual pathogen

Country Status (3)

Country Link
CN (1) CN108251514A (zh)
CA (1) CA3059288C (zh)
WO (1) WO2019153675A1 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108251514A (zh) * 2018-02-08 2018-07-06 中国农业大学 一种双重致病菌的比色传感新方法
CN109082459A (zh) * 2018-07-18 2018-12-25 桂林理工大学 Taq DNA连接酶和量子点信号放大的DNA电化学传感器检测单核苷酸多态性的方法
CN109207615B (zh) * 2018-10-12 2021-06-29 广东省生态环境技术研究所 一种免标记荧光检测金黄色葡萄球菌mecA基因的方法及检测试剂盒
CN111154843B (zh) * 2020-04-07 2020-07-28 中国农业大学 一种基于超速pcr与功能核酸显色的定量检测方法
CN113493823A (zh) * 2020-04-07 2021-10-12 中国农业大学 一种基于超速pcr与功能核酸的可视化检测方法
CN114214392B (zh) * 2021-11-17 2024-06-11 广东省科学院生态环境与土壤研究所 一种基于核酸四面体的冠状病毒定量检测方法及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1274847A (zh) * 2000-06-02 2000-11-29 上海长征医院 标记信号放大探针的制备方法
CN102827836B (zh) * 2012-06-11 2014-03-12 中国科学院成都生物研究所 一种寡核苷酸探针以及用其对靶分子进行检测的方法
CN103993100B (zh) * 2014-06-17 2017-02-15 东南大学 一种提高基因连接测序准确性的方法
CN105018474A (zh) * 2014-08-22 2015-11-04 江苏省原子医学研究所 一种基于g-四链体-氯血红素dna酶的探针及其应用
CN108251514A (zh) * 2018-02-08 2018-07-06 中国农业大学 一种双重致病菌的比色传感新方法

Also Published As

Publication number Publication date
WO2019153675A1 (zh) 2019-08-15
CA3059288A1 (en) 2019-08-15
CN108251514A (zh) 2018-07-06

Similar Documents

Publication Publication Date Title
CA3059288C (en) Novel colorimetric sensing method for dual pathogen
CN109207567B (zh) 一种基于适配体和链置换扩增反应对金黄色葡萄球菌的测定方法
Wang et al. Specific and rapid detection of foodborne Salmonella by loop-mediated isothermal amplification method
Wang et al. Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Listeria monocytogenes
Furukawa et al. Highly sensitive real-time PCR assay for quantification of toxic cyanobacteria based on microcystin synthetase A gene
CN109321669B (zh) 一种基于嵌合体序列设计和分子信标的荧光检测金黄色葡萄球菌的方法
CN106434917A (zh) 一种金黄色葡萄球菌的lamp引物组及检测试剂盒与使用方法
CA2692633A1 (en) Method for the simultaneous detection of multiple nucleic acid sequences in a sample
Wei et al. Rapid and sensitive detection of Vibrio parahaemolyticus in sea foods by electrochemiluminescence polymerase chain reaction method
US20090226895A1 (en) Method of detecting vibrio parahaemolyticus via real-time PCR-hybridization
EP3902929A1 (en) Fast and portable microfluidic detection system as an alternative to salmonella's classical culture method
WO2013128397A1 (en) Real-time pcr detection of streptococcus pyogenes
Hu et al. Rapid and visual detection of Vibrio parahaemolyticus in aquatic foods using blaCARB-17 gene-based loop-mediated isothermal amplification with lateral flow dipstick (LAMP-LFD)
Zeng et al. A polymerase chain reaction based lateral flow test strip with propidium monoazide for detection of viable Vibrio parahaemolyticus in codfish
Yuan et al. A facile and pragmatic electrochemical biosensing strategy for ultrasensitive detection of DNA in real sample based on defective T junction induced transcription amplification
CA3059246C (en) New method for visual quantitative detection of dual heavy metal ions
KR20170030746A (ko) Lamp를 이용한 살모넬라 검출용 프라이머 및 그 용도
CN102719542A (zh) 霍乱弧菌(vc)的实时荧光核酸恒温扩增检测试剂盒
Liu et al. Visual assay of Escherichia coli O157: H7 based on an isothermal strand displacement and hybrid chain reaction amplification strategy
AU2020101818A4 (en) A novel LAMP method, primer set and kit for SNP detection
Li et al. Robust and highly specific fluorescence sensing of Salmonella typhimurium based on dual-functional phi29 DNA polymerase-mediated isothermal circular strand displacement polymerization
Pang et al. Cyclic strand displacement polymerase reaction to turn-on molecular beacons for rapid detection of Staphylococcus aureus
KR20170030190A (ko) Lamp를 이용한 클로스트리디움 퍼프린젠스 검출용 프라이머 및 그 용도
CN110387429B (zh) 用于大肠杆菌o157:h7血清型致病菌检测的试剂、试剂盒及应用
CN114231647A (zh) 基于可视化法的滚环等温扩增技术检测食源性致病菌金黄色葡萄球菌的方法及其应用

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20191007