CA3055729A1 - Extracellular matrix-producing composition using mast4 gene and preparation method therefor - Google Patents

Extracellular matrix-producing composition using mast4 gene and preparation method therefor Download PDF

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Publication number
CA3055729A1
CA3055729A1 CA3055729A CA3055729A CA3055729A1 CA 3055729 A1 CA3055729 A1 CA 3055729A1 CA 3055729 A CA3055729 A CA 3055729A CA 3055729 A CA3055729 A CA 3055729A CA 3055729 A1 CA3055729 A1 CA 3055729A1
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Prior art keywords
composition
mast4
fragment
protein
eukaryotic cells
Prior art date
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Pending
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CA3055729A
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English (en)
French (fr)
Inventor
Han Sung Jung
Satoru Takahashi
Seong Jin Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kim Yeung Won June
Kim Ypsse
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Kim Yeung Won June
Kim Ypsse
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Application filed by Kim Yeung Won June, Kim Ypsse filed Critical Kim Yeung Won June
Priority claimed from PCT/KR2018/002763 external-priority patent/WO2018164507A2/ko
Publication of CA3055729A1 publication Critical patent/CA3055729A1/en
Pending legal-status Critical Current

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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655Chondrocytes; Cartilage
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18Growth factors; Growth regulators
    • A61K38/1841Transforming growth factor [TGF]
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1031Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656Adult fibroblasts
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11001Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/07Animals genetically altered by homologous recombination
    • A01K2217/075Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/105Murine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • A01K2267/035Animal model for multifactorial diseases
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65MicroRNA
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases [EC 2.]
    • C12N2501/727Kinases (EC 2.7.)
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    • C12N2800/00Nucleic acids vectors
    • C12N2800/80Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA3055729A 2017-03-08 2018-03-08 Extracellular matrix-producing composition using mast4 gene and preparation method therefor Pending CA3055729A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR10-2017-0029607 2017-03-08
KR20170029607 2017-03-08
KR10-2018-0027111 2018-03-07
KR1020180027111A KR102588627B1 (ko) 2017-03-08 2018-03-07 Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법
PCT/KR2018/002763 WO2018164507A2 (ko) 2017-03-08 2018-03-08 Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법

Publications (1)

Publication Number Publication Date
CA3055729A1 true CA3055729A1 (en) 2018-09-13

Family

ID=63718648

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CA3055729A Pending CA3055729A1 (en) 2017-03-08 2018-03-08 Extracellular matrix-producing composition using mast4 gene and preparation method therefor

Country Status (7)

Country Link
US (2) US11180573B2 (enExample)
EP (1) EP3594324A4 (enExample)
JP (3) JP7154238B2 (enExample)
KR (1) KR102588627B1 (enExample)
CN (1) CN110612348B (enExample)
AU (2) AU2018231559A1 (enExample)
CA (1) CA3055729A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021146566A1 (en) * 2020-01-16 2021-07-22 The Johns Hopkins University Engineered fibroblasts as cell therapy to treat cancer via tumor stroma stabilization

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022020785A2 (en) * 2020-07-23 2022-01-27 Orthobio Therapeutics, Inc. Gene editing to improve joint function
AU2023215206A1 (en) * 2022-02-04 2024-08-22 Pyunggang Kim Method of making bone and cartilage

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US5055298A (en) * 1987-12-01 1991-10-08 Chemisches Laboratorium Dr. Kurt Richter Gmbh Cosmetic composition comprising an extracellular connective tissue matrix
US6528052B1 (en) 2000-09-29 2003-03-04 The Board Of Trustees Of The Leland Stanford Junior University Method for in vivo ex vivo and in vitro repair and regeneration of cartilage and collagen and bone remodeling
WO2005121316A1 (en) * 2004-06-11 2005-12-22 Bernard O'brien Institute Of Microsurgery Tissue material and muscle derived matrix
KR100614903B1 (ko) * 2004-08-06 2006-08-25 테고사이언스 (주) 케라티노사이트 및/또는 섬유아세포를 함유하는 화장용조성물
EP1894581A1 (en) 2006-08-31 2008-03-05 CellCoTec B.V. Repair of cartilage tissue using a matrix gel containing chondrocytes
JP2011526585A (ja) * 2008-03-31 2011-10-13 スカルセル テラピュティク 皮膚の老化の美容的処置法
MX353186B (es) 2009-09-03 2018-01-05 Genentech Inc Metodos para el tratamiento, diagnosis y monitoreo de artritis reumatoide.
WO2012083363A1 (en) * 2010-12-22 2012-06-28 Murdoch Childrens Research Institute A method of treatment
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WO2015035395A1 (en) 2013-09-09 2015-03-12 Figene, Llc Gene therapy for the regeneration of chondrocytes or cartilage type cells
CN105916979A (zh) * 2013-11-14 2016-08-31 德玛奇实验室公司 成纤维细胞混合物及其制备和使用方法
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021146566A1 (en) * 2020-01-16 2021-07-22 The Johns Hopkins University Engineered fibroblasts as cell therapy to treat cancer via tumor stroma stabilization

Also Published As

Publication number Publication date
JP7498239B2 (ja) 2024-06-11
US11180573B2 (en) 2021-11-23
AU2018231559A1 (en) 2019-10-31
AU2023222944A1 (en) 2023-11-23
JP7154238B2 (ja) 2022-10-17
JP2024116197A (ja) 2024-08-27
EP3594324A4 (en) 2021-01-20
CN110612348B (zh) 2024-02-09
JP2020513849A (ja) 2020-05-21
US20200239596A1 (en) 2020-07-30
EP3594324A2 (en) 2020-01-15
US20220220221A1 (en) 2022-07-14
CN110612348A (zh) 2019-12-24
KR102588627B1 (ko) 2023-10-16
JP2022188178A (ja) 2022-12-20
KR20180103015A (ko) 2018-09-18

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