KR102588627B1 - Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법 - Google Patents
Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법 Download PDFInfo
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- KR102588627B1 KR102588627B1 KR1020180027111A KR20180027111A KR102588627B1 KR 102588627 B1 KR102588627 B1 KR 102588627B1 KR 1020180027111 A KR1020180027111 A KR 1020180027111A KR 20180027111 A KR20180027111 A KR 20180027111A KR 102588627 B1 KR102588627 B1 KR 102588627B1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
- A61K38/1841—Transforming growth factor [TGF]
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/11—Protein-serine/threonine kinases (2.7.11)
- C12Y207/11001—Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/035—Animal model for multifactorial diseases
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/65—MicroRNA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
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- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Rheumatology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2018231559A AU2018231559A1 (en) | 2017-03-08 | 2018-03-08 | Extracellular matrix-producing composition using MAST4 gene and preparation method therefor |
| CN201880030519.0A CN110612348B (zh) | 2017-03-08 | 2018-03-08 | 用于使用mast4基因产生细胞外基质的组合物及其制备方法 |
| US16/492,477 US11180573B2 (en) | 2017-03-08 | 2018-03-08 | Extracellular matrix-producing composition using MAST4 gene and preparation method therefor |
| JP2019571194A JP7154238B2 (ja) | 2017-03-08 | 2018-03-08 | Mast4遺伝子を利用した細胞外基質生産用組成物及びその製造方法 |
| CA3055729A CA3055729A1 (en) | 2017-03-08 | 2018-03-08 | Extracellular matrix-producing composition using mast4 gene and preparation method therefor |
| PCT/KR2018/002763 WO2018164507A2 (ko) | 2017-03-08 | 2018-03-08 | Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법 |
| EP18764604.7A EP3594324A4 (en) | 2017-03-08 | 2018-03-08 | EXTRACELLULAR MATRIX-PRODUCING COMPOSITION WITH MAST4-GEN AND MANUFACTURING METHOD FOR IT |
| US17/494,765 US20220220221A1 (en) | 2017-03-08 | 2021-10-05 | Extracellular matrix-producing composition using mast4 gene and preparation method therefor |
| JP2022159928A JP7498239B2 (ja) | 2017-03-08 | 2022-10-04 | Mast4遺伝子を利用した細胞外基質生産用組成物及びその製造方法 |
| AU2023222944A AU2023222944A1 (en) | 2017-03-08 | 2023-08-31 | Extracellular matrix-producing composition using MAST4 gene and preparation method therefor |
| JP2024087859A JP2024116197A (ja) | 2017-03-08 | 2024-05-30 | Mast4遺伝子を利用した細胞外基質生産用組成物及びその製造方法 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170029607 | 2017-03-08 | ||
| KR20170029607 | 2017-03-08 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20180103015A KR20180103015A (ko) | 2018-09-18 |
| KR102588627B1 true KR102588627B1 (ko) | 2023-10-16 |
Family
ID=63718648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020180027111A Active KR102588627B1 (ko) | 2017-03-08 | 2018-03-07 | Mast4 유전자를 이용한 세포외 기질 생산용 조성물 및 그 제조방법 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11180573B2 (enExample) |
| EP (1) | EP3594324A4 (enExample) |
| JP (3) | JP7154238B2 (enExample) |
| KR (1) | KR102588627B1 (enExample) |
| CN (1) | CN110612348B (enExample) |
| AU (2) | AU2018231559A1 (enExample) |
| CA (1) | CA3055729A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021146566A1 (en) * | 2020-01-16 | 2021-07-22 | The Johns Hopkins University | Engineered fibroblasts as cell therapy to treat cancer via tumor stroma stabilization |
| US20240299579A1 (en) * | 2020-07-23 | 2024-09-12 | Orthobio Therapeutics, Inc. | Gene editing to improve joint function |
| US20250136991A1 (en) * | 2022-02-04 | 2025-05-01 | Kim Seong Jin | Method of making bone and cartilage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140341887A1 (en) | 2009-09-03 | 2014-11-20 | Genentech, Inc. | Methods for treating, diagnosing, and monitoring rheumatoid arthritis |
| WO2015035395A1 (en) | 2013-09-09 | 2015-03-12 | Figene, Llc | Gene therapy for the regeneration of chondrocytes or cartilage type cells |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5055298A (en) * | 1987-12-01 | 1991-10-08 | Chemisches Laboratorium Dr. Kurt Richter Gmbh | Cosmetic composition comprising an extracellular connective tissue matrix |
| US6528052B1 (en) * | 2000-09-29 | 2003-03-04 | The Board Of Trustees Of The Leland Stanford Junior University | Method for in vivo ex vivo and in vitro repair and regeneration of cartilage and collagen and bone remodeling |
| US20060153797A1 (en) * | 2004-06-11 | 2006-07-13 | Bortolotto Susan K | Tissue material and matrix |
| KR100614903B1 (ko) * | 2004-08-06 | 2006-08-25 | 테고사이언스 (주) | 케라티노사이트 및/또는 섬유아세포를 함유하는 화장용조성물 |
| EP1894581A1 (en) * | 2006-08-31 | 2008-03-05 | CellCoTec B.V. | Repair of cartilage tissue using a matrix gel containing chondrocytes |
| CA2719981A1 (en) * | 2008-03-31 | 2009-10-08 | Scarcell Therapeutics | Method for the cosmetic treatment of skin ageing |
| US10301620B2 (en) * | 2010-12-22 | 2019-05-28 | Murdoch Childrens Research Institute | Method of treatment |
| EP2753692B1 (en) * | 2011-09-07 | 2019-03-27 | 3-D Matrix Ltd. | Microrna-based methods and assays for osteosarcoma |
| US9878056B2 (en) | 2012-04-02 | 2018-01-30 | Modernatx, Inc. | Modified polynucleotides for the production of cosmetic proteins and peptides |
| KR102039311B1 (ko) | 2012-09-07 | 2019-11-04 | 주식회사 마크로젠 | Axl을 포함하는 융합 단백질 및 이를 포함하는 암 진단용 조성물 |
| CA2930556A1 (en) * | 2013-11-14 | 2015-05-21 | Dermarche Labs, Llc | Fibroblast mixtures and methods of making and using the same |
| CA2986441A1 (en) | 2015-06-12 | 2016-12-15 | Dana-Farber Cancer Institute, Inc. | Combination therapy of transcription inhibitors and kinase inhibitors |
| AU2017207445B2 (en) * | 2016-01-14 | 2023-12-07 | Spinalcyte, Llc | Cellular blend for the regeneration of chondrocytes or cartilage type cells |
-
2018
- 2018-03-07 KR KR1020180027111A patent/KR102588627B1/ko active Active
- 2018-03-08 JP JP2019571194A patent/JP7154238B2/ja active Active
- 2018-03-08 CN CN201880030519.0A patent/CN110612348B/zh active Active
- 2018-03-08 CA CA3055729A patent/CA3055729A1/en active Pending
- 2018-03-08 US US16/492,477 patent/US11180573B2/en active Active
- 2018-03-08 AU AU2018231559A patent/AU2018231559A1/en not_active Abandoned
- 2018-03-08 EP EP18764604.7A patent/EP3594324A4/en active Pending
-
2021
- 2021-10-05 US US17/494,765 patent/US20220220221A1/en not_active Abandoned
-
2022
- 2022-10-04 JP JP2022159928A patent/JP7498239B2/ja active Active
-
2023
- 2023-08-31 AU AU2023222944A patent/AU2023222944A1/en active Pending
-
2024
- 2024-05-30 JP JP2024087859A patent/JP2024116197A/ja active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140341887A1 (en) | 2009-09-03 | 2014-11-20 | Genentech, Inc. | Methods for treating, diagnosing, and monitoring rheumatoid arthritis |
| WO2015035395A1 (en) | 2013-09-09 | 2015-03-12 | Figene, Llc | Gene therapy for the regeneration of chondrocytes or cartilage type cells |
| US20160220699A1 (en) | 2013-09-09 | 2016-08-04 | Figene, Llc | Gene therapy for the regeneration of chondrocytes or cartilage type cells |
Also Published As
| Publication number | Publication date |
|---|---|
| JP7498239B2 (ja) | 2024-06-11 |
| JP2024116197A (ja) | 2024-08-27 |
| JP2020513849A (ja) | 2020-05-21 |
| AU2023222944A1 (en) | 2023-11-23 |
| EP3594324A4 (en) | 2021-01-20 |
| AU2018231559A1 (en) | 2019-10-31 |
| EP3594324A2 (en) | 2020-01-15 |
| JP7154238B2 (ja) | 2022-10-17 |
| JP2022188178A (ja) | 2022-12-20 |
| CN110612348A (zh) | 2019-12-24 |
| CN110612348B (zh) | 2024-02-09 |
| KR20180103015A (ko) | 2018-09-18 |
| US20220220221A1 (en) | 2022-07-14 |
| US20200239596A1 (en) | 2020-07-30 |
| CA3055729A1 (en) | 2018-09-13 |
| US11180573B2 (en) | 2021-11-23 |
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