CA3024295C - IMPROVEMENTS TO OR RELATING TO NUCLEIC ACID AMPLIFICATION PROCESSES - Google Patents

IMPROVEMENTS TO OR RELATING TO NUCLEIC ACID AMPLIFICATION PROCESSES

Info

Publication number
CA3024295C
CA3024295C CA3024295A CA3024295A CA3024295C CA 3024295 C CA3024295 C CA 3024295C CA 3024295 A CA3024295 A CA 3024295A CA 3024295 A CA3024295 A CA 3024295A CA 3024295 C CA3024295 C CA 3024295C
Authority
CA
Canada
Prior art keywords
temperature
amplification
target
reaction
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3024295A
Other languages
English (en)
French (fr)
Other versions
CA3024295A1 (en
Inventor
Jarrod Provins
Daiwei Shen
Bryan KRAYNACK
Original Assignee
LumiraDx UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56891177&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA3024295(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by LumiraDx UK Ltd filed Critical LumiraDx UK Ltd
Publication of CA3024295A1 publication Critical patent/CA3024295A1/en
Application granted granted Critical
Publication of CA3024295C publication Critical patent/CA3024295C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/307—Single strand endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3024295A 2016-06-30 2017-06-30 IMPROVEMENTS TO OR RELATING TO NUCLEIC ACID AMPLIFICATION PROCESSES Active CA3024295C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1611469.6 2016-06-30
GBGB1611469.6A GB201611469D0 (en) 2016-06-30 2016-06-30 Improvements in or relating to nucleic acid amplification processes
PCT/GB2017/051927 WO2018002649A1 (en) 2016-06-30 2017-06-30 Improvements in or relating to nucleic acid amplification processes

Publications (2)

Publication Number Publication Date
CA3024295A1 CA3024295A1 (en) 2018-01-04
CA3024295C true CA3024295C (en) 2024-09-24

Family

ID=56891177

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3024295A Active CA3024295C (en) 2016-06-30 2017-06-30 IMPROVEMENTS TO OR RELATING TO NUCLEIC ACID AMPLIFICATION PROCESSES

Country Status (15)

Country Link
US (2) US11591643B2 (cg-RX-API-DMAC7.html)
EP (1) EP3478853B2 (cg-RX-API-DMAC7.html)
JP (2) JP6957527B2 (cg-RX-API-DMAC7.html)
KR (1) KR102392010B1 (cg-RX-API-DMAC7.html)
CN (1) CN109642251B (cg-RX-API-DMAC7.html)
AU (1) AU2017287852B2 (cg-RX-API-DMAC7.html)
CA (1) CA3024295C (cg-RX-API-DMAC7.html)
ES (1) ES2841624T3 (cg-RX-API-DMAC7.html)
GB (1) GB201611469D0 (cg-RX-API-DMAC7.html)
IL (1) IL263870A (cg-RX-API-DMAC7.html)
MX (1) MX2018015421A (cg-RX-API-DMAC7.html)
RU (1) RU2760915C2 (cg-RX-API-DMAC7.html)
SG (1) SG11201809927XA (cg-RX-API-DMAC7.html)
WO (1) WO2018002649A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201807460B (cg-RX-API-DMAC7.html)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) * 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201812149D0 (en) 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method
GB201915346D0 (en) 2019-06-21 2019-12-04 Lumiradx Tech Ltd Improvements in or relating to nicking enzymes
BR112022014535A2 (pt) 2020-01-25 2022-09-20 Sense Biodetection Ltd Detecção de vírus
PT3969618T (pt) * 2020-03-09 2023-11-15 Illumina Inc Métodos para sequenciação de polinucleótidos
EP4347868A1 (en) 2021-05-27 2024-04-10 Lumiradx Uk Ltd Improvements in or relating to digestion of reaction products
US12331157B2 (en) 2021-09-16 2025-06-17 Circ, LLC Method of forming a polyester from a regenerated diacid formed from depolymerization of a waste material
US20230082247A1 (en) * 2021-09-16 2023-03-16 Circ, LLC Method of aging regenerated diacid crystals

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2017821C1 (ru) * 1990-10-10 1994-08-15 Анатолий Михайлович Онищенко Способ амплификации днк и устройство для его осуществления
US5432065A (en) 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US20020025555A1 (en) * 1997-03-06 2002-02-28 Janice Au-Young GPCR diagnostic for brain cancer
WO1999037805A1 (en) 1998-01-27 1999-07-29 Cytocell Limited Method for detection of nucleic acid target sequences involving in vitro transcription from an rna promoter
ATE286981T1 (de) 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
US20010047200A1 (en) 1999-10-13 2001-11-29 Raymond Sun Non-foreshortening intraluminal prosthesis
NO314091B1 (no) * 2000-01-12 2003-01-27 Biotec Pharmacon Asa Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
DE60115811T2 (de) 2000-10-25 2006-08-03 Roche Diagnostics Gmbh Amplifizierung unter Verwendung von modifizierten Primern
GB0101397D0 (en) 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US20030211506A1 (en) 2001-06-01 2003-11-13 Huimin Kong N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
WO2003008622A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Exponential nucleic acid amplification using nicking endonucleases
AU2002217428A1 (en) 2001-12-04 2003-06-17 Bio3 Research Srl Mixture of two partially denaturating agents for the preparation of pcr buffer solutions
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2004055193A1 (en) 2002-12-18 2004-07-01 Dingbang Xu Pcr method and application by transnormal low thermo-denaturation temperature
JP4567673B2 (ja) 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
EP3985125A1 (en) 2004-06-01 2022-04-20 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
EP1771250B1 (en) 2004-06-04 2011-01-26 Abacus Diagnostica OY Temperature control of reaction vessel, system with reaction vessel and use of system
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7537917B2 (en) 2006-03-31 2009-05-26 Collins Michael J Microwave assisted PCR amplification of DNA
US20090092967A1 (en) 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
DK2574681T3 (en) 2007-03-28 2016-07-04 Signal Diagnostics System and method for high-resolution analysis of nucleic acids to detect sequence variations
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2009042291A2 (en) * 2007-08-03 2009-04-02 Igor Kutyavin Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking
FI20085450A0 (fi) 2008-05-14 2008-05-14 Arto Orpana Menetelmä DNA-juosteen syntetisoimiseksi
GB0915664D0 (en) 2009-09-08 2009-10-07 Enigma Diagnostics Ltd Reaction method
AU2010298202B2 (en) 2009-09-25 2015-11-05 Alere San Diego, Inc. Detection of nucleic acids in crude matrices
US9501070B2 (en) * 2010-04-20 2016-11-22 Qiagen Instruments Ag Temperature control method and apparatus
WO2012077819A1 (ja) 2010-12-10 2012-06-14 国立大学法人 東京工業大学 標的核酸の検出方法及びキット
EP2652148B1 (en) 2010-12-17 2016-11-30 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
EP2699700B8 (en) 2011-04-20 2016-08-24 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
US8343752B2 (en) * 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
US9096897B2 (en) * 2012-04-09 2015-08-04 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
CA2876159C (en) 2012-06-08 2022-09-06 Ionian Technologies, Inc. Nucleic acid amplifications
EP3094753B1 (en) 2014-01-15 2019-10-16 Abbott Laboratories Covered sequence conversion dna and detection methods
ES2759339T3 (es) 2014-10-14 2020-05-08 Abbott Lab Conversión de secuencia y ADN amplificador de señal que tiene ácidos nucleicos bloqueados y métodos de detección que usan los mismos
WO2016103234A1 (en) 2014-12-24 2016-06-30 Abbott Japan Co., Ltd. Detection method based on signal amplifier dna cascade reactions with target extension
GB201513128D0 (en) 2015-07-24 2015-09-09 Sense Biodetection Ltd Nucleic acid detection method
EP3334834B1 (en) 2015-08-12 2020-05-13 Circulogene Theranostics, LLC Method of preparing cell free nucleic acid molecules by in situ amplification
US10329601B2 (en) 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB201701262D0 (en) 2017-01-25 2017-03-08 Sense Biodetection Ltd Nucleic acid detection method
RU2020100221A (ru) 2017-08-31 2021-09-30 Иониан Текнолоджис, Ллс Реакция амплификации с разрывом и удлинением (near) разновидностей респираторно-синцитиального вируса
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201812149D0 (en) 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method

Also Published As

Publication number Publication date
AU2017287852A1 (en) 2018-11-22
US20190226015A1 (en) 2019-07-25
ZA201807460B (en) 2021-06-30
MX2018015421A (es) 2019-11-28
JP6957527B2 (ja) 2021-11-02
RU2760915C2 (ru) 2021-12-01
SG11201809927XA (en) 2019-01-30
KR20190024890A (ko) 2019-03-08
US11591643B2 (en) 2023-02-28
EP3478853B2 (en) 2025-11-26
AU2017287852B2 (en) 2022-11-24
WO2018002649A1 (en) 2018-01-04
RU2019101504A3 (cg-RX-API-DMAC7.html) 2020-11-30
CN109642251A (zh) 2019-04-16
GB201611469D0 (en) 2016-08-17
CA3024295A1 (en) 2018-01-04
ES2841624T3 (es) 2021-07-08
EP3478853A1 (en) 2019-05-08
CN109642251B (zh) 2022-08-19
BR112018075801A2 (pt) 2019-03-26
RU2019101504A (ru) 2020-07-30
EP3478853B1 (en) 2020-10-07
US20230340579A1 (en) 2023-10-26
JP2019519231A (ja) 2019-07-11
IL263870A (en) 2019-01-31
JP2022017268A (ja) 2022-01-25
KR102392010B1 (ko) 2022-04-27

Similar Documents

Publication Publication Date Title
US20230340579A1 (en) Nucleic acid amplification processes
EP3587587B1 (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
CA2983819A1 (en) Amplification with primers of limited nucleotide composition
US20240026433A1 (en) Amplification of nucleic acids
TWI754652B (zh) 核酸擴增方法中或相關之改良
HK40000996A (en) Improvements in or relating to nucleic acid amplification processes
HK40000996B (en) Improvements in or relating to nucleic acid amplification processes
HK40036522B (en) Improvements in or relating to amplification of nucleic acids
HK40036522A (en) Improvements in or relating to amplification of nucleic acids
BR112018075801B1 (pt) Método para realizar uma reação de amplificação de ácido nucleico não isotérmico e aparelho para realizar o método

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111

EEER Examination request

Effective date: 20220111