RU2760915C2 - Усовершенствования в процессах амплификации нуклеиновой кислоты или связанные с таковыми - Google Patents

Усовершенствования в процессах амплификации нуклеиновой кислоты или связанные с таковыми Download PDF

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RU2760915C2
RU2760915C2 RU2019101504A RU2019101504A RU2760915C2 RU 2760915 C2 RU2760915 C2 RU 2760915C2 RU 2019101504 A RU2019101504 A RU 2019101504A RU 2019101504 A RU2019101504 A RU 2019101504A RU 2760915 C2 RU2760915 C2 RU 2760915C2
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temperature
amplification
target
polymerase
primers
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RU2019101504A
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RU2019101504A3 (cg-RX-API-DMAC7.html
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Джаррод ПРОВИНС
Дайвэй ШЭНЬ
Брайан КРЭЙНЭК
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ЛЮМИРАДЭКС Юкей ЛТД
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/307—Single strand endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
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  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
RU2019101504A 2016-06-30 2017-06-30 Усовершенствования в процессах амплификации нуклеиновой кислоты или связанные с таковыми RU2760915C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1611469.6A GB201611469D0 (en) 2016-06-30 2016-06-30 Improvements in or relating to nucleic acid amplification processes
GB1611469.6 2016-06-30
PCT/GB2017/051927 WO2018002649A1 (en) 2016-06-30 2017-06-30 Improvements in or relating to nucleic acid amplification processes

Publications (3)

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RU2019101504A RU2019101504A (ru) 2020-07-30
RU2019101504A3 RU2019101504A3 (cg-RX-API-DMAC7.html) 2020-11-30
RU2760915C2 true RU2760915C2 (ru) 2021-12-01

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Country Link
US (2) US11591643B2 (cg-RX-API-DMAC7.html)
EP (1) EP3478853B2 (cg-RX-API-DMAC7.html)
JP (2) JP6957527B2 (cg-RX-API-DMAC7.html)
KR (1) KR102392010B1 (cg-RX-API-DMAC7.html)
CN (1) CN109642251B (cg-RX-API-DMAC7.html)
AU (1) AU2017287852B2 (cg-RX-API-DMAC7.html)
CA (1) CA3024295C (cg-RX-API-DMAC7.html)
ES (1) ES2841624T3 (cg-RX-API-DMAC7.html)
GB (1) GB201611469D0 (cg-RX-API-DMAC7.html)
IL (1) IL263870A (cg-RX-API-DMAC7.html)
MX (1) MX2018015421A (cg-RX-API-DMAC7.html)
RU (1) RU2760915C2 (cg-RX-API-DMAC7.html)
SG (1) SG11201809927XA (cg-RX-API-DMAC7.html)
WO (1) WO2018002649A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201807460B (cg-RX-API-DMAC7.html)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) * 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201812149D0 (en) 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method
GB201915346D0 (en) 2019-06-21 2019-12-04 Lumiradx Tech Ltd Improvements in or relating to nicking enzymes
EP4093882A1 (en) 2020-01-25 2022-11-30 Sense Biodetection Limited Virus detection
US20220316003A1 (en) * 2020-03-09 2022-10-06 Illumina, Inc. Methods for sequencing polynucleotides
EP4347868A1 (en) 2021-05-27 2024-04-10 Lumiradx Uk Ltd Improvements in or relating to digestion of reaction products
EP4402119A4 (en) * 2021-09-16 2025-10-15 Circ Llc AGING PROCESS FOR REGENERATED DIACID CRYSTALS
TW202330758A (zh) 2021-09-16 2023-08-01 美商賽克股份有限公司 從由廢棄材料解聚合所形成再生二酸形成聚酯之方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2017821C1 (ru) * 1990-10-10 1994-08-15 Анатолий Михайлович Онищенко Способ амплификации днк и устройство для его осуществления
RU2260055C2 (ru) * 1999-09-28 2005-09-10 Роше Диагностикс Гмбх Способ амплификации днк и композиция для его осуществления
US20090017453A1 (en) * 2007-07-14 2009-01-15 Maples Brian K Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2011030145A1 (en) * 2009-09-08 2011-03-17 Enigma Diagnostics Limited Nucleic acid amplification employing temperature oscillation

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5432065A (en) 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US20020025555A1 (en) * 1997-03-06 2002-02-28 Janice Au-Young GPCR diagnostic for brain cancer
WO1999037805A1 (en) 1998-01-27 1999-07-29 Cytocell Limited Method for detection of nucleic acid target sequences involving in vitro transcription from an rna promoter
US20010047200A1 (en) 1999-10-13 2001-11-29 Raymond Sun Non-foreshortening intraluminal prosthesis
NO314091B1 (no) * 2000-01-12 2003-01-27 Biotec Pharmacon Asa Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
DE60115811T2 (de) 2000-10-25 2006-08-03 Roche Diagnostics Gmbh Amplifizierung unter Verwendung von modifizierten Primern
GB0101397D0 (en) 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US20030211506A1 (en) 2001-06-01 2003-11-13 Huimin Kong N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
JP2004535814A (ja) * 2001-07-15 2004-12-02 ケック グラデュエイト インスティテュート ニック形成エンドヌクレアーゼを使用する指数関数的核酸増幅
WO2003048393A1 (en) 2001-12-04 2003-06-12 Bio3 Research Srl Mixture of two partially denaturating agents for the preparation of pcr buffer solutions
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2004055193A1 (en) 2002-12-18 2004-07-01 Dingbang Xu Pcr method and application by transnormal low thermo-denaturation temperature
EP1615942B1 (en) 2003-04-01 2016-03-16 Luminex Corporation Polymerase inhibitor and method of using same
EP1759012B1 (en) 2004-06-01 2013-05-22 Alere San Diego, Inc. Recombinase polymerase amplification
WO2005118144A1 (en) * 2004-06-04 2005-12-15 Abacus Diagnostica Oy Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7537917B2 (en) 2006-03-31 2009-05-26 Collins Michael J Microwave assisted PCR amplification of DNA
WO2008002920A2 (en) 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
CN103952470B (zh) 2007-03-28 2017-11-10 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
US8143006B2 (en) * 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
FI20085450A0 (fi) 2008-05-14 2008-05-14 Arto Orpana Menetelmä DNA-juosteen syntetisoimiseksi
CA2775143A1 (en) 2009-09-25 2011-03-31 Alere San Diego, Inc. Detection of nucleic acids in crude matrices
EP2560759B1 (en) * 2010-04-20 2020-02-12 QIAGEN GmbH Temperature control method and apparatus
JP6126381B2 (ja) 2010-12-10 2017-05-10 国立大学法人東京工業大学 標的核酸の検出方法及びキット
EP3564392B1 (en) 2010-12-17 2021-11-24 Life Technologies Corporation Methods for nucleic acid amplification
CA2856304C (en) 2011-04-20 2017-05-16 Mesa Tech International, Inc. Oscillating amplification reaction for nucleic acids
US8343752B2 (en) * 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
JP5801513B2 (ja) * 2012-04-09 2015-10-28 エンバイロロジックス インコーポレイテッド 試料中の核酸配列を定量するための組成物および方法
WO2013185081A1 (en) 2012-06-08 2013-12-12 Ionian Technologies, Inc Nucleic acid amplifications
WO2015114469A2 (en) 2014-01-15 2015-08-06 Abbott Japan Co., Ltd Covered sequence conversion dna and detection methods
EP3207159B1 (en) 2014-10-14 2019-11-20 Abbott Laboratories Sequence conversion and signal amplifier dna having poly dna spacer sequences and detection methods using same
JP6840080B2 (ja) 2014-12-24 2021-03-10 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ 標的伸長を伴うシグナル増幅ｄｎａカスケード反応に基づく検出方法
GB201513128D0 (en) 2015-07-24 2015-09-09 Sense Biodetection Ltd Nucleic acid detection method
AU2016306688A1 (en) 2015-08-12 2018-03-29 Circulogene Theranostics, Llc Method of preparing cell free nucleic acid molecules by in situ amplification
US10329601B2 (en) 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB201701262D0 (en) 2017-01-25 2017-03-08 Sense Biodetection Ltd Nucleic acid detection method
BR112020004071A2 (pt) 2017-08-31 2020-09-24 Ionian Technologies, Inc. reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201812149D0 (en) 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2017821C1 (ru) * 1990-10-10 1994-08-15 Анатолий Михайлович Онищенко Способ амплификации днк и устройство для его осуществления
RU2260055C2 (ru) * 1999-09-28 2005-09-10 Роше Диагностикс Гмбх Способ амплификации днк и композиция для его осуществления
US20090017453A1 (en) * 2007-07-14 2009-01-15 Maples Brian K Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2011030145A1 (en) * 2009-09-08 2011-03-17 Enigma Diagnostics Limited Nucleic acid amplification employing temperature oscillation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JONEJA ARIC, HUANG XIAOHUA. Linear nicking endonuclease-mediated strand-displacement DNA amplification. Analytical biochemistry, 2011, Volume 414, Issue 1, pp.58-69. *
WANG DE-GUO et al. Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification, Molecules, 2015, vol.20, pp.6048-6059. *

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KR102392010B1 (ko) 2022-04-27
JP6957527B2 (ja) 2021-11-02
ZA201807460B (en) 2021-06-30
IL263870A (en) 2019-01-31
AU2017287852A1 (en) 2018-11-22
JP2022017268A (ja) 2022-01-25
RU2019101504A (ru) 2020-07-30
US20190226015A1 (en) 2019-07-25
US11591643B2 (en) 2023-02-28
SG11201809927XA (en) 2019-01-30
EP3478853A1 (en) 2019-05-08
CA3024295A1 (en) 2018-01-04
ES2841624T3 (es) 2021-07-08
WO2018002649A1 (en) 2018-01-04
CN109642251B (zh) 2022-08-19
GB201611469D0 (en) 2016-08-17
EP3478853B1 (en) 2020-10-07
KR20190024890A (ko) 2019-03-08
EP3478853B2 (en) 2025-11-26
AU2017287852B2 (en) 2022-11-24
MX2018015421A (es) 2019-11-28
CN109642251A (zh) 2019-04-16
CA3024295C (en) 2024-09-24
RU2019101504A3 (cg-RX-API-DMAC7.html) 2020-11-30
BR112018075801A2 (pt) 2019-03-26
US20230340579A1 (en) 2023-10-26
JP2019519231A (ja) 2019-07-11

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