CA2990092A1 - Targeted enrichment of long nucleotide sequences using microfluidic partitioning - Google Patents
Targeted enrichment of long nucleotide sequences using microfluidic partitioning Download PDFInfo
- Publication number
- CA2990092A1 CA2990092A1 CA2990092A CA2990092A CA2990092A1 CA 2990092 A1 CA2990092 A1 CA 2990092A1 CA 2990092 A CA2990092 A CA 2990092A CA 2990092 A CA2990092 A CA 2990092A CA 2990092 A1 CA2990092 A1 CA 2990092A1
- Authority
- CA
- Canada
- Prior art keywords
- droplets
- dna
- target dna
- target
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Dispersion Chemistry (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562185111P | 2015-06-26 | 2015-06-26 | |
| US62/185,111 | 2015-06-26 | ||
| DKPA201570449 | 2015-07-07 | ||
| DKPA201570449 | 2015-07-07 | ||
| PCT/EP2016/064719 WO2016207379A1 (en) | 2015-06-26 | 2016-06-24 | Targeted enrichment of long nucleotide sequences using microfluidic partitioning |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2990092A1 true CA2990092A1 (en) | 2016-12-29 |
Family
ID=57584736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2990092A Pending CA2990092A1 (en) | 2015-06-26 | 2016-06-24 | Targeted enrichment of long nucleotide sequences using microfluidic partitioning |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10738354B2 (enExample) |
| EP (2) | EP3314012B1 (enExample) |
| JP (1) | JP6936155B2 (enExample) |
| AU (1) | AU2016282350A1 (enExample) |
| CA (1) | CA2990092A1 (enExample) |
| DK (1) | DK3770271T3 (enExample) |
| WO (1) | WO2016207379A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704080B2 (en) * | 2014-06-11 | 2020-07-07 | Samplix Aps | Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS) |
| WO2018221513A1 (ja) * | 2017-05-31 | 2018-12-06 | キヤノン株式会社 | 油性組成物、およびそれを用いた分析方法 |
| DK182109B1 (en) | 2019-08-02 | 2025-08-18 | Samplix Aps | System and method for amplifying nucleic acids from single cells |
| US20210214769A1 (en) * | 2020-01-13 | 2021-07-15 | Fluent Biosciences Inc. | Methods and systems for amplifying low concentrations of nucleic acids |
| DK181994B1 (en) * | 2021-09-28 | 2025-05-15 | Samplix Aps | Instrument for sorting of particles |
| JP7734312B2 (ja) | 2020-12-01 | 2025-09-05 | サンプリックス エーピーエス | 粒子を分類するためのシステムおよび方法 |
| DK181862B1 (en) | 2022-03-21 | 2025-02-24 | Samplix Aps | Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2977455B1 (en) | 2009-06-15 | 2020-04-15 | Complete Genomics, Inc. | Method for long fragment read sequencing |
| US20120164633A1 (en) | 2010-12-27 | 2012-06-28 | Ibis Biosciences, Inc. | Digital droplet sequencing |
| AU2013366478A1 (en) | 2012-12-21 | 2015-07-09 | Samplix S.A.R.L. | Probability-directed isolation of nucleotide sequences (pins) |
| US20140303005A1 (en) | 2013-04-05 | 2014-10-09 | Raindance Technologies, Inc. | Rare cell analysis after negative selection |
| WO2014172373A2 (en) | 2013-04-19 | 2014-10-23 | Raindance Technologies, Inc | Digital analyte analysis |
| US10704080B2 (en) * | 2014-06-11 | 2020-07-07 | Samplix Aps | Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS) |
| US10697007B2 (en) * | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
-
2016
- 2016-06-24 EP EP16732595.0A patent/EP3314012B1/en active Active
- 2016-06-24 DK DK20191574.1T patent/DK3770271T3/da active
- 2016-06-24 AU AU2016282350A patent/AU2016282350A1/en not_active Abandoned
- 2016-06-24 WO PCT/EP2016/064719 patent/WO2016207379A1/en not_active Ceased
- 2016-06-24 EP EP20191574.1A patent/EP3770271B1/en active Active
- 2016-06-24 CA CA2990092A patent/CA2990092A1/en active Pending
- 2016-06-24 JP JP2017566728A patent/JP6936155B2/ja active Active
- 2016-06-24 US US15/739,541 patent/US10738354B2/en active Active
-
2020
- 2020-07-06 US US16/921,103 patent/US11572584B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3314012A1 (en) | 2018-05-02 |
| JP2018518968A (ja) | 2018-07-19 |
| US10738354B2 (en) | 2020-08-11 |
| US20200332353A1 (en) | 2020-10-22 |
| WO2016207379A1 (en) | 2016-12-29 |
| EP3770271B1 (en) | 2023-02-08 |
| US20180179589A1 (en) | 2018-06-28 |
| EP3314012B1 (en) | 2020-09-09 |
| JP6936155B2 (ja) | 2021-09-15 |
| EP3770271A1 (en) | 2021-01-27 |
| US11572584B2 (en) | 2023-02-07 |
| AU2016282350A1 (en) | 2018-01-18 |
| DK3770271T3 (da) | 2023-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |
|
| EEER | Examination request |
Effective date: 20210623 |