CA2966442C - Sequencing from multiple primers to increase data rate and density - Google Patents

Sequencing from multiple primers to increase data rate and density Download PDF

Info

Publication number
CA2966442C
CA2966442C CA2966442A CA2966442A CA2966442C CA 2966442 C CA2966442 C CA 2966442C CA 2966442 A CA2966442 A CA 2966442A CA 2966442 A CA2966442 A CA 2966442A CA 2966442 C CA2966442 C CA 2966442C
Authority
CA
Canada
Prior art keywords
nucleic acid
primers
nucleotide
extension
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2966442A
Other languages
English (en)
French (fr)
Other versions
CA2966442A1 (en
Inventor
Jonathan Mark Boutell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Cambridge Ltd
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of CA2966442A1 publication Critical patent/CA2966442A1/en
Application granted granted Critical
Publication of CA2966442C publication Critical patent/CA2966442C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30072—Microarray; Biochip, DNA array; Well plate

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
CA2966442A 2014-11-05 2015-11-04 Sequencing from multiple primers to increase data rate and density Active CA2966442C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1419731.3A GB201419731D0 (en) 2014-11-05 2014-11-05 Sequencing from multiple primers to increase data rate and density
GB1419731.3 2014-11-05
PCT/GB2015/053324 WO2016071689A1 (en) 2014-11-05 2015-11-04 Sequencing from multiple primers to increase data rate and density

Publications (2)

Publication Number Publication Date
CA2966442A1 CA2966442A1 (en) 2016-05-12
CA2966442C true CA2966442C (en) 2024-03-12

Family

ID=52118764

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2966442A Active CA2966442C (en) 2014-11-05 2015-11-04 Sequencing from multiple primers to increase data rate and density

Country Status (11)

Country Link
US (3) US20170298430A1 (show.php)
EP (2) EP3215638B1 (show.php)
JP (1) JP6789935B2 (show.php)
CN (2) CN107257862B (show.php)
AU (2) AU2015341568B2 (show.php)
CA (1) CA2966442C (show.php)
DK (1) DK3215638T3 (show.php)
ES (1) ES2889875T3 (show.php)
GB (1) GB201419731D0 (show.php)
SG (1) SG11201703629XA (show.php)
WO (1) WO2016071689A1 (show.php)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017190018A1 (en) 2016-04-29 2017-11-02 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP4361287A3 (en) * 2016-09-28 2024-08-07 Life Technologies Corporation Methods for sequencing nucleic acids using termination chemistry
EP3562962B1 (en) 2016-12-30 2022-01-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
DE102017218849A1 (de) * 2017-10-23 2019-04-25 Robert Bosch Gmbh Reaktionsträger für eine mikrofluidische Vorrichtung und Verfahren zur Bestimmung einer Nukleotidsequenz
CN113249455A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种基因测序中获得背景信号的方法
WO2023114392A1 (en) * 2021-12-16 2023-06-22 Ultima Genomics, Inc. Systems and methods for sequencing with multi-priming
WO2023175026A1 (en) * 2022-03-15 2023-09-21 Illumina, Inc. Methods of determining sequence information
EP4341426A2 (en) * 2022-03-15 2024-03-27 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
EP4621066A4 (en) * 2022-12-12 2026-01-21 Mgi Tech Co Ltd MULTI-MATRIX SYNCHRONOUS NUCLEIC ACID SEQUENCING METHOD AND ITS USE
CN117721191B (zh) * 2024-02-07 2024-05-10 深圳赛陆医疗科技有限公司 基因测序方法、测序装置、可读存储介质和基因测序系统

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
WO1989012696A1 (en) 1988-06-24 1989-12-28 Amgen Inc. Method and reagents for detecting nucleic acid sequences
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
AU635105B2 (en) 1990-01-26 1993-03-11 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
CA2182517C (en) 1994-02-07 2001-08-21 Theo Nikiforov Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
CA2386791A1 (en) * 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU8288101A (en) 2000-07-07 2002-01-21 Visigen Biotechnologies Inc Real-time sequence determination
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2003056030A2 (en) * 2001-11-08 2003-07-10 The Johns Hopkins University Methods and systems of nucleic acid sequencing
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP1530578B1 (en) 2002-08-23 2013-03-13 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
EP1636337A4 (en) 2003-06-20 2007-07-04 Illumina Inc METHODS AND COMPOSITIONS USEFUL FOR THE AMPLIFICATION AND GENOTYPING OF THE GENOME
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ES2949821T3 (es) 2004-01-07 2023-10-03 Illumina Cambridge Ltd Matrices moleculares
US7302146B2 (en) 2004-09-17 2007-11-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3373174A1 (en) 2006-03-31 2018-09-12 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
JP5622392B2 (ja) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション 大規模ｆｅｔアレイを用いた分析物測定のための方法および装置
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2725107B1 (en) * 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified ddNTPs and nucleic acid comprising inosine with reversible terminators
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20110195410A1 (en) * 2009-12-07 2011-08-11 Gene Check, Inc. Compositions and methods related to solid phase sequence detection and genotyping
CN102140534A (zh) * 2010-12-15 2011-08-03 深圳华大基因科技有限公司 一种hbv基因的核苷酸突变位点的检测方法
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2012135664A2 (en) * 2011-03-31 2012-10-04 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
EP3981886A1 (en) 2011-09-23 2022-04-13 Illumina, Inc. Compositions for nucleic acid sequencing
IN2014DN07992A (show.php) 2012-04-03 2015-05-01 Illumina Inc
AU2014284560B2 (en) * 2013-07-03 2020-03-26 Illumina, Inc. Sequencing by orthogonal synthesis

Also Published As

Publication number Publication date
JP2017533709A (ja) 2017-11-16
HK1243465A1 (en) 2018-07-13
AU2015341568A1 (en) 2017-05-25
WO2016071689A1 (en) 2016-05-12
EP3974538A1 (en) 2022-03-30
JP6789935B2 (ja) 2020-11-25
AU2015341568B2 (en) 2021-09-16
DK3215638T3 (da) 2021-10-18
CN107257862A (zh) 2017-10-17
CN107257862B (zh) 2021-08-03
US20200392573A1 (en) 2020-12-17
US20170298430A1 (en) 2017-10-19
US11555218B2 (en) 2023-01-17
CN113564238A (zh) 2021-10-29
US20230160006A1 (en) 2023-05-25
EP3215638B1 (en) 2021-08-11
EP3215638A1 (en) 2017-09-13
CA2966442A1 (en) 2016-05-12
GB201419731D0 (en) 2014-12-17
CN113564238B (zh) 2025-08-05
SG11201703629XA (en) 2017-06-29
AU2021286342B2 (en) 2024-05-30
AU2021286342A1 (en) 2022-01-20
ES2889875T3 (es) 2022-01-14

Similar Documents

Publication Publication Date Title
AU2021286342B2 (en) Sequencing from multiple primers to increase data rate and density
US12123056B2 (en) Surface concatemerization of templates
CN107849598B (zh) 簇中的表面引物的增强利用
EP3320111A1 (en) Sample preparation for nucleic acid amplification
HK40066434A (en) Sequencing from multiple primers to increase data rate and density
HK1243465B (en) Sequencing from multiple primers to increase data rate and density
HK40046037A (en) Surface concatamerization of templates
HK40046037B (en) Surface concatamerization of templates
HK1244846B (en) Surface concatamerization of templates
HK1246366B (en) Enhanced utilization of surface primers in clusters

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030

EEER Examination request

Effective date: 20201030