CA2954889A1 - Cytochrome p450s and uses thereof - Google Patents

Cytochrome p450s and uses thereof Download PDF

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Publication number
CA2954889A1
CA2954889A1 CA2954889A CA2954889A CA2954889A1 CA 2954889 A1 CA2954889 A1 CA 2954889A1 CA 2954889 A CA2954889 A CA 2954889A CA 2954889 A CA2954889 A CA 2954889A CA 2954889 A1 CA2954889 A1 CA 2954889A1
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Prior art keywords
plant
cell
expression
cells
seq
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA2954889A
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English (en)
French (fr)
Inventor
Joseph Chappell
Lyle F. Ralston
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University of Kentucky Research Foundation
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University of Kentucky Research Foundation
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Publication of CA2954889A1 publication Critical patent/CA2954889A1/en
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13078—Ent-kaurene oxidase (1.14.13.78)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13119—5-Epiaristolochene 1,3-dihydroxylase (1.14.13.119)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2954889A 2001-03-09 2002-03-08 Cytochrome p450s and uses thereof Expired - Lifetime CA2954889A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US27442101P 2001-03-09 2001-03-09
US60/274,421 2001-03-09
US27559701P 2001-03-13 2001-03-13
US60/275,597 2001-03-13
CA2440278A CA2440278C (en) 2001-03-09 2002-03-08 Cytochrome p450s and uses thereof

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2440278A Division CA2440278C (en) 2001-03-09 2002-03-08 Cytochrome p450s and uses thereof

Publications (1)

Publication Number Publication Date
CA2954889A1 true CA2954889A1 (en) 2002-09-19

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CA2954889A Expired - Lifetime CA2954889A1 (en) 2001-03-09 2002-03-08 Cytochrome p450s and uses thereof
CA2440278A Expired - Lifetime CA2440278C (en) 2001-03-09 2002-03-08 Cytochrome p450s and uses thereof

Family Applications After (1)

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Country Status (7)

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US (7) US7405057B2 (cg-RX-API-DMAC10.html)
EP (1) EP1373476B1 (cg-RX-API-DMAC10.html)
JP (1) JP2004532014A (cg-RX-API-DMAC10.html)
AT (1) ATE513046T1 (cg-RX-API-DMAC10.html)
AU (1) AU2002244262B2 (cg-RX-API-DMAC10.html)
CA (2) CA2954889A1 (cg-RX-API-DMAC10.html)
WO (1) WO2002072758A2 (cg-RX-API-DMAC10.html)

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US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
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KR20140004760A (ko) 2011-02-28 2014-01-13 노쓰 캐롤라이나 스테이트 유니버시티 담배 근교배 식물 ncbex1f, ncbex1ms 및 nc ex90
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WO2015134438A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US9596823B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
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CN115058437A (zh) * 2022-05-23 2022-09-16 浙江理工大学 高效促进丹参酮积累的基因SmCYP82U4及其用途

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Also Published As

Publication number Publication date
WO2002072758A2 (en) 2002-09-19
CA2440278C (en) 2017-02-28
US20160024530A1 (en) 2016-01-28
US8722363B2 (en) 2014-05-13
US20140212939A1 (en) 2014-07-31
EP1373476A2 (en) 2004-01-02
CA2440278A1 (en) 2002-09-19
US20130330793A1 (en) 2013-12-12
US20100120110A1 (en) 2010-05-13
US20110318797A1 (en) 2011-12-29
US20030166255A1 (en) 2003-09-04
US8445231B2 (en) 2013-05-21
JP2004532014A (ja) 2004-10-21
US10184119B2 (en) 2019-01-22
WO2002072758A3 (en) 2003-10-30
US10184118B2 (en) 2019-01-22
AU2002244262B2 (en) 2007-07-12
US8263362B2 (en) 2012-09-11
US7405057B2 (en) 2008-07-29
US20160032256A1 (en) 2016-02-04
EP1373476B1 (en) 2011-06-15
EP1373476A4 (en) 2004-10-27
ATE513046T1 (de) 2011-07-15

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