CA2943952A1 - Chemical methods for producing tagged nucleotides - Google Patents
Chemical methods for producing tagged nucleotides Download PDFInfo
- Publication number
- CA2943952A1 CA2943952A1 CA2943952A CA2943952A CA2943952A1 CA 2943952 A1 CA2943952 A1 CA 2943952A1 CA 2943952 A CA2943952 A CA 2943952A CA 2943952 A CA2943952 A CA 2943952A CA 2943952 A1 CA2943952 A1 CA 2943952A1
- Authority
- CA
- Canada
- Prior art keywords
- dt6p
- nucleotide
- tag
- tagged
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/02—Heterocyclic radicals containing only nitrogen as ring hetero atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461969628P | 2014-03-24 | 2014-03-24 | |
| US61/969,628 | 2014-03-24 | ||
| PCT/US2015/022063 WO2015148402A1 (en) | 2014-03-24 | 2015-03-23 | Chemical methods for producing tagged nucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2943952A1 true CA2943952A1 (en) | 2015-10-01 |
Family
ID=54196272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2943952A Abandoned CA2943952A1 (en) | 2014-03-24 | 2015-03-23 | Chemical methods for producing tagged nucleotides |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10240195B2 (enExample) |
| EP (1) | EP3122759B1 (enExample) |
| JP (1) | JP6805425B2 (enExample) |
| CN (1) | CN106715453B (enExample) |
| AU (1) | AU2015236392A1 (enExample) |
| CA (1) | CA2943952A1 (enExample) |
| MA (1) | MA39774A (enExample) |
| WO (1) | WO2015148402A1 (enExample) |
Families Citing this family (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| ATE356222T1 (de) | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| WO2007002204A2 (en) | 2005-06-21 | 2007-01-04 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compostions |
| US7982029B2 (en) | 2005-10-31 | 2011-07-19 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods |
| US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
| EP2652153B1 (en) | 2010-12-17 | 2017-07-05 | The Trustees of Columbia University in the City of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
| US9624539B2 (en) | 2011-05-23 | 2017-04-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using Raman and infrared spectroscopy detection |
| US10246479B2 (en) | 2012-04-09 | 2019-04-02 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| EP2864502B1 (en) | 2012-06-20 | 2019-10-23 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| CN105102627B (zh) | 2013-03-15 | 2018-10-19 | 纽约哥伦比亚大学理事会 | 用于检测样品中多种预定化合物的方法 |
| CA2943952A1 (en) | 2014-03-24 | 2015-10-01 | The Trustees Of Columbia University In The City Of New York | Chemical methods for producing tagged nucleotides |
| WO2016144973A1 (en) | 2015-03-09 | 2016-09-15 | The Trustees Of Columbia University In The City Of New York | Pore-forming protein conjugate compositions and methods |
| US11085076B2 (en) | 2015-09-28 | 2021-08-10 | The Trustees Of Columbia University In The City Of New York | Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis |
| CN108521782A (zh) * | 2015-10-21 | 2018-09-11 | 豪夫迈·罗氏有限公司 | 含氟聚合物作为疏水层以支持用于纳米孔的脂质双层形成的用途 |
| US20180327828A1 (en) * | 2015-11-18 | 2018-11-15 | The Trustees Of Columbia University In The City Of New York | Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators |
| CN108603180A (zh) | 2015-11-25 | 2018-09-28 | 豪夫迈·罗氏有限公司 | 聚合酶复合体的纯化 |
| WO2017125565A1 (en) * | 2016-01-21 | 2017-07-27 | Genia Technologies, Inc. | Nanopore sequencing complexes |
| JP7280590B2 (ja) * | 2016-01-28 | 2023-05-24 | ロズウェル バイオテクノロジーズ，インコーポレイテッド | 大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置 |
| CN108699540A (zh) * | 2016-02-29 | 2018-10-23 | 吉尼亚科技公司 | 用于纳米孔测序的聚合酶-模板复合物 |
| JP6959251B2 (ja) * | 2016-03-24 | 2021-11-02 | ジェニア・テクノロジーズ・インコーポレイテッド | ナノポアシステムに有用な部位特異的バイオコンジュゲーション方法および組成物 |
| US10883140B2 (en) * | 2016-04-21 | 2021-01-05 | President And Fellows Of Harvard College | Method and system of nanopore-based information encoding |
| EP3463381A4 (en) | 2016-05-23 | 2020-01-01 | The Trustees of Columbia University in the City of New York | NUCLEOTIDE DERIVATIVES AND METHOD FOR USE THEREOF |
| US10655174B2 (en) | 2016-05-27 | 2020-05-19 | Roche Sequencing Solutions, Inc. | Tagged multi-nucleotides useful for nucleic acid sequencing |
| CN109844513A (zh) * | 2016-08-01 | 2019-06-04 | 罗斯韦尔生物技术股份有限公司 | 用于分子电子传感器的经修饰的核苷酸三磷酸 |
| US10669580B2 (en) | 2016-08-26 | 2020-06-02 | Roche Sequencing Solutions, Inc. | Tagged nucleotides useful for nanopore detection |
| US11591647B2 (en) | 2017-03-06 | 2023-02-28 | Singular Genomics Systems, Inc. | Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps |
| CN110709089B (zh) | 2017-03-28 | 2024-12-06 | 哥伦比亚大学董事会 | 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物 |
| WO2018191389A1 (en) * | 2017-04-12 | 2018-10-18 | Roche Sequencing Solutions, Inc. | A method for sequencing reaction with tagged nucleoside obtained via pictet spengler reaction |
| CN107190067B (zh) * | 2017-06-13 | 2019-12-13 | 厦门艾德生物医药科技股份有限公司 | 一种改进的二代测序用随机标签接头制作方法 |
| WO2019040546A1 (en) | 2017-08-23 | 2019-02-28 | Roche Sequencing Solutions, Inc | ENZYME SCREENING METHODS |
| EP3682020B1 (en) | 2017-09-15 | 2025-09-03 | Illumina, Inc. | Sequence-detection system |
| KR102034304B1 (ko) * | 2017-12-29 | 2019-10-18 | 한국과학기술연구원 | 나노포어 구조체를 사용한 폴리펩티드의 아미노산 서열을 분석하는 방법 |
| JP6938690B2 (ja) | 2018-02-16 | 2021-09-22 | イラミーナ インコーポレーテッド | 電荷タグ付きヌクレオチドおよびその使用方法 |
| US12215124B2 (en) | 2018-02-21 | 2025-02-04 | Singular Genomics Systems, Inc. | Fluorinated nucleotides and uses thereof |
| WO2019166457A1 (en) | 2018-02-28 | 2019-09-06 | F. Hoffmann-La Roche Ag | Tagged nucleoside compounds useful for nanopore detection |
| WO2019178393A1 (en) | 2018-03-15 | 2019-09-19 | The Trustees Of Columbia University In The City Of New York | Nucleotide analogues and use thereof for nucleic acid sequencing and analysis |
| CN112534063A (zh) | 2018-05-22 | 2021-03-19 | 安序源有限公司 | 用于核酸测序的方法、系统和组合物 |
| CN112204154B (zh) | 2018-05-28 | 2024-06-25 | 豪夫迈·罗氏有限公司 | Dna-孔隙-聚合酶复合物的酶促富集 |
| CN112236521A (zh) * | 2018-06-05 | 2021-01-15 | 慕尼黑工业大学 | 用于稳定核酸纳米结构的新方法 |
| CN113227772B (zh) | 2018-07-23 | 2024-11-01 | 纽约市哥伦比亚大学信托人 | 用于生物标志物检测的单分子电子多重纳米孔免疫测定 |
| WO2020043653A2 (en) | 2018-08-28 | 2020-03-05 | F. Hoffmann-La Roche Ag | Ruthenium-containing electrodes |
| CN109134541B (zh) * | 2018-09-10 | 2020-10-27 | 伯科生物医学科技（北京）有限公司 | 长链生物素标记物及其制备方法和用途 |
| WO2020086834A1 (en) | 2018-10-25 | 2020-04-30 | Singular Genomics Systems, Inc. | Nucleotide analogues |
| ES2988083T3 (es) * | 2018-12-19 | 2024-11-19 | Hoffmann La Roche | Nucleótidos protegidos reversiblemente en 3' para secuenciación por síntesis usando nanoporos |
| CN113453692A (zh) | 2019-01-08 | 2021-09-28 | 奇异基因组学系统公司 | 核苷酸可裂解接头和其用途 |
| CN113710686A (zh) | 2019-02-19 | 2021-11-26 | 阿尔缇玛基因组学公司 | 用于光学检测和测序的接头和方法 |
| EP3972976A4 (en) * | 2019-05-23 | 2023-07-19 | Stratos Genomics Inc. | TRANSLOCATION CONTROL ELEMENTS, REPORTER CODES AND OTHER TRANSLOCATION CONTROL MEANS FOR USE IN NANOPOORE SEQUENCING |
| KR102811825B1 (ko) | 2019-06-21 | 2025-05-26 | 써모 피셔 사이언티픽 발틱스 유에이비 | 차세대 시퀀싱 라이브러리를 제조하기 위한 핵산 표지화에 유용한 올리고뉴클레오티드-테더된 트리포스페이트 뉴클레오티드 |
| TW202120695A (zh) * | 2019-08-21 | 2021-06-01 | 美商伊路米納有限公司 | 用聚合酶將核苷酸接附至聚核苷酸 |
| CA3164781A1 (en) * | 2019-12-18 | 2021-06-24 | F. Hoffmann-La Roche Ag | Methods of sequencing by synthesis using a consecutive labeling scheme |
| WO2021156370A1 (en) | 2020-02-06 | 2021-08-12 | F. Hoffmann-La Roche Ag | Compositions that reduce template threading into a nanopore |
| US11807851B1 (en) | 2020-02-18 | 2023-11-07 | Ultima Genomics, Inc. | Modified polynucleotides and uses thereof |
| US20230249178A1 (en) | 2020-07-08 | 2023-08-10 | Roche Sequencing Solutions, Inc. | Split-pool synthesis apparatus and methods of performing split-pool synthesis |
| CN116075596A (zh) | 2020-08-07 | 2023-05-05 | 牛津纳米孔科技公开有限公司 | 鉴定核酸条形码的方法 |
| WO2022033998A1 (en) | 2020-08-11 | 2022-02-17 | F. Hoffmann-La Roche Ag | Nucleoside-5'-oligophosphates tagged with positively-charged polymers, nanopores incorporating negative charges, and methods and systems using the same |
| JP2023545478A (ja) | 2020-10-15 | 2023-10-30 | カパ バイオシステムズ，インコーポレイティド | 次世代配列決定ライブラリ調製のための電気泳動デバイスおよび方法 |
| WO2022194764A1 (en) | 2021-03-15 | 2022-09-22 | F. Hoffmann-La Roche Ag | Targeted next-generation sequencing via anchored primer extension |
| WO2022200485A1 (en) | 2021-03-26 | 2022-09-29 | F. Hoffmann-La Roche Ag | Hybridization buffer formulations |
| EP4314337A1 (en) | 2021-04-01 | 2024-02-07 | F. Hoffmann-La Roche AG | Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing |
| EP4355757A1 (en) | 2021-06-17 | 2024-04-24 | F. Hoffmann-La Roche AG | Nucleoside-5 -oligophosphates having a cationically-modified nucleobase |
| CN118056019A (zh) | 2021-06-17 | 2024-05-17 | 豪夫迈·罗氏有限公司 | 具有穿过通道的带负电荷的聚合物的工程化纳米孔 |
| US12435369B2 (en) | 2021-08-13 | 2025-10-07 | Singular Genomcis Systems, Inc. | Nucleotide analogs and uses thereof |
| CN113736778B (zh) * | 2021-09-14 | 2025-05-09 | 成都齐碳科技有限公司 | 测序接头、构建方法、纳米孔建库试剂盒及应用 |
| CN118318049A (zh) | 2021-09-28 | 2024-07-09 | 安序源有限公司 | 用于处理核酸样品的方法以及核酸样品的组合物 |
| WO2023213766A1 (en) | 2022-05-02 | 2023-11-09 | F. Hoffmann-La Roche Ag | Compositions and methods that reduce prussian blue formation during nanopore sequencing |
| JP2025521678A (ja) | 2022-06-30 | 2025-07-10 | カパ バイオシステムズ，インコーポレイティド | 古細菌ヒストン様タンパク質を使用したタグメンテーション配列決定ライブラリーインサートサイズの制御 |
| CN119895049A (zh) | 2022-09-02 | 2025-04-25 | 豪夫迈·罗氏有限公司 | 对下一代靶标富集性能的改进 |
| EP4599075A2 (en) * | 2022-10-05 | 2025-08-13 | Axbio Inc. | Compositions, methods, and systems for detecting nucleotides |
| EP4689180A1 (en) | 2023-03-30 | 2026-02-11 | F. Hoffmann-La Roche AG | Modulation of target molecule-lipid bilayer interactions |
| CN121368715A (zh) | 2023-04-13 | 2026-01-20 | 文塔纳医疗系统公司 | 用于固定的实体瘤的增殖测定 |
| EP4705012A1 (en) | 2023-05-04 | 2026-03-11 | F. Hoffmann-La Roche AG | Hybrid triblock copolymer membrane compositions and methods for nanopore sequencing |
| CN121057766A (zh) | 2023-05-04 | 2025-12-02 | 豪夫迈·罗氏有限公司 | 经修饰的三嵌段共聚物化合物及其使用方法 |
| WO2024256358A2 (en) | 2023-06-14 | 2024-12-19 | F. Hoffmann-La Roche Ag | Accelerated marking of 5-formyl cytosine and use in nucleic acid methylation sequencing |
| GB202310278D0 (en) * | 2023-07-05 | 2023-08-16 | Navinci Diagnostics Ab | Stabilisation of phi29 polymerase |
| CN118409092B (zh) * | 2023-09-21 | 2024-10-18 | 上海市肺科医院（上海市职业病防治院） | 一种用于干扰素γ检测的核酸适体功能化磁珠及其制备方法和试剂盒 |
| US20250109433A1 (en) * | 2023-09-22 | 2025-04-03 | Illumina, Inc. | Macromolecule blocks for nanopore sequencing |
| WO2025193604A1 (en) | 2024-03-11 | 2025-09-18 | Ventana Medical Systems, Inc. | Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers |
| WO2025210056A1 (en) | 2024-04-03 | 2025-10-09 | Roche Sequencing Solutions, Inc. | In vitro amplification of dna methylation patterns |
| WO2025240460A2 (en) | 2024-05-14 | 2025-11-20 | Roche Molecular Systems, Inc. | Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent |
| WO2026050541A1 (en) | 2024-08-28 | 2026-03-05 | Roche Sequencing Solutions, Inc. | Methods and systems for allele-specific copy number calling |
| WO2026050289A1 (en) | 2024-08-29 | 2026-03-05 | Roche Sequencing Solutions, Inc. | Pre-and post-sequencing cleaning protocols for nanopore sequencing |
| WO2026050290A1 (en) | 2024-08-29 | 2026-03-05 | Roche Sequencing Solutions, Inc. | Lipid composition and methods of use in a nanopore sequencing assay |
| WO2026050292A1 (en) | 2024-08-29 | 2026-03-05 | Roche Sequencing Solutions, Inc. | Optimized reagent introduction during a nanopore sequencing assay |
| WO2026060095A1 (en) | 2024-09-16 | 2026-03-19 | Roche Sequencing Solutions, Inc. | Single cell rna sequencing methods and systems |
| WO2026082489A1 (en) | 2024-10-18 | 2026-04-23 | F. Hoffmann-La Roche Ag | Universal pcr to boost molecule recovery in cell free dna library preparation and target enrichment workflows |
| WO2026087220A1 (en) | 2024-10-22 | 2026-04-30 | F. Hoffmann-La Roche Ag | Preparation of dna libraries for next generation sequencing using y-adapters modified with phosphorothioate bonds |
| WO2026093331A1 (en) | 2024-10-28 | 2026-05-07 | F. Hoffmann-La Roche Ag | Hairpin adapter constructs and primers to generate next-generation sequencing library molecules |
Family Cites Families (131)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH055425Y2 (enExample) | 1986-05-20 | 1993-02-12 | ||
| US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| JPH07508342A (ja) | 1992-04-22 | 1995-09-14 | エコール ポリテクニーク フェデラル ドゥ ローザンヌ（エーペーエフエル） | 脂質膜センサー |
| GB9315847D0 (en) | 1993-07-30 | 1993-09-15 | Isis Innovation | Tag reagent and assay method |
| US20120160687A1 (en) | 1995-03-17 | 2012-06-28 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6046005A (en) | 1997-01-15 | 2000-04-04 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group |
| US5876936A (en) | 1997-01-15 | 1999-03-02 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators |
| US5804386A (en) | 1997-01-15 | 1998-09-08 | Incyte Pharmaceuticals, Inc. | Sets of labeled energy transfer fluorescent primers and their use in multi component analysis |
| US6485703B1 (en) | 1998-07-31 | 2002-11-26 | The Texas A&M University System | Compositions and methods for analyte detection |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| JP4638043B2 (ja) * | 1998-12-14 | 2011-02-23 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法 |
| US20030054360A1 (en) | 1999-01-19 | 2003-03-20 | Larry Gold | Method and apparatus for the automated generation of nucleic acid ligands |
| US6399335B1 (en) | 1999-11-16 | 2002-06-04 | Advanced Research And Technology Institute, Inc. | γ-phosphoester nucleoside triphosphates |
| US6383749B2 (en) | 1999-12-02 | 2002-05-07 | Clontech Laboratories, Inc. | Methods of labeling nucleic acids for use in array based hybridization assays |
| EP2083015B1 (en) | 2000-02-11 | 2016-04-06 | The Texas A & M University System | Biosensor compositions and methods of use |
| US6616895B2 (en) | 2000-03-23 | 2003-09-09 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6936702B2 (en) | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
| AU2001268291A1 (en) | 2000-06-07 | 2001-12-17 | Li-Cor, Inc. | Charge-switch nucleotides |
| EP1307469B1 (en) | 2000-08-03 | 2008-01-23 | Boehringer Mannheim Gmbh | Nucleic acid binding compounds containing pyrazolo¬3,4-d pyrimidine analogues of purin-2,6-diamine and their uses |
| US20060057565A1 (en) | 2000-09-11 | 2006-03-16 | Jingyue Ju | Combinatorial fluorescence energy transfer tags and uses thereof |
| US6627748B1 (en) | 2000-09-11 | 2003-09-30 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses |
| CA2421582A1 (en) | 2000-09-11 | 2002-03-21 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and uses thereof |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| ATE356222T1 (de) | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| US20030027140A1 (en) | 2001-03-30 | 2003-02-06 | Jingyue Ju | High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry |
| US7041812B2 (en) | 2001-08-29 | 2006-05-09 | Amersham Biosciences Corp | Labeled nucleoside polyphosphates |
| DK2431377T3 (en) | 2001-10-26 | 2017-08-14 | Noxxon Pharma Ag | Modified L-nucleic acids |
| WO2003064621A2 (en) | 2002-02-01 | 2003-08-07 | Ambion, Inc. | HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES |
| US7074597B2 (en) | 2002-07-12 | 2006-07-11 | The Trustees Of Columbia University In The City Of New York | Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry |
| US20090186343A1 (en) * | 2003-01-28 | 2009-07-23 | Visigen Biotechnologies, Inc. | Methods for preparing modified biomolecules, modified biomolecules and methods for using same |
| JP4896708B2 (ja) * | 2003-02-05 | 2012-03-14 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 新規リンカー類を有する末端リン酸標識ヌクレオチド類 |
| EP1597602A4 (en) | 2003-02-05 | 2009-07-22 | Ge Healthcare Bio Sciences | SOLID PHASE SEQUENCING |
| US7365178B2 (en) * | 2003-04-01 | 2008-04-29 | Activx Biosciences, Inc. | Acyl-nucleotide probes and methods of their synthesis and use in proteomic analysis |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| US7019346B2 (en) | 2003-12-23 | 2006-03-28 | Intel Corporation | Capacitor having an anodic metal oxide substrate |
| US20050148087A1 (en) * | 2004-01-05 | 2005-07-07 | Applera Corporation | Isobarically labeled analytes and fragment ions derived therefrom |
| CA2557818A1 (en) | 2004-03-03 | 2005-09-15 | The Trustees Of Columbia University In The City Of New York | Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry |
| US7279337B2 (en) | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US20050239134A1 (en) | 2004-04-21 | 2005-10-27 | Board Of Regents, The University Of Texas System | Combinatorial selection of phosphorothioate single-stranded DNA aptamers for TGF-beta-1 protein |
| GB2431013B (en) | 2004-07-23 | 2008-05-21 | Electronic Bio Sciences Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| CA2577079C (en) | 2004-08-13 | 2014-05-20 | President And Fellows Of Harvard College | An ultra high-throughput opti-nanopore dna readout platform |
| US20060105461A1 (en) | 2004-10-22 | 2006-05-18 | May Tom-Moy | Nanopore analysis system |
| WO2007084103A2 (en) | 2004-12-21 | 2007-07-26 | The Texas A & M University System | High temperature ion channels and pores |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| WO2007002204A2 (en) | 2005-06-21 | 2007-01-04 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compostions |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| WO2007041621A2 (en) | 2005-10-03 | 2007-04-12 | Xingsheng Sean Ling | Hybridization assisted nanopore sequencing |
| US7397232B2 (en) | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
| US7982029B2 (en) | 2005-10-31 | 2011-07-19 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods |
| WO2007053719A2 (en) | 2005-10-31 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
| EP1963530B1 (en) | 2005-12-22 | 2011-07-27 | Pacific Biosciences of California, Inc. | Active surface coupled polymerases |
| US20070298511A1 (en) | 2006-04-27 | 2007-12-27 | The Texas A&M University System | Nanopore sensor system |
| US7777505B2 (en) | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| US8889348B2 (en) | 2006-06-07 | 2014-11-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by nanopore using modified nucleotides |
| EP1867638A1 (de) * | 2006-06-16 | 2007-12-19 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Verfahren zur kovalenten Verknüpfung zweier Moleküle mittels Diels-Alder-Reaktion mit inversem Elektronenbedarf |
| DE112007002932B4 (de) | 2006-12-01 | 2015-08-06 | The Trustees Of Columbia University In The City Of New York | Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren |
| US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| US20100196203A1 (en) | 2007-02-20 | 2010-08-05 | Gurdial Singh Sanghera | Formation of Lipid Bilayers |
| WO2008124107A1 (en) | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| US20090073293A1 (en) | 2007-06-27 | 2009-03-19 | Yoel Yaffe | CMOS image sensors with increased dynamic range and methods of operating the same |
| WO2009046094A1 (en) | 2007-10-01 | 2009-04-09 | Nabsys, Inc. | Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment |
| EP2205765B1 (en) | 2007-10-02 | 2012-09-12 | President and Fellows of Harvard College | Capture, recapture, and trapping of molecules with a nanopore |
| EP4310194A3 (en) | 2007-10-19 | 2024-10-16 | The Trustees of Columbia University in the City of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
| US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
| US20110166043A1 (en) * | 2007-11-14 | 2011-07-07 | Jon Owen Nagy | Biomarker detection-2 |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US20110165652A1 (en) * | 2008-01-14 | 2011-07-07 | Life Technologies Corporation | Compositions, methods and systems for single molecule sequencing |
| DE102008006990A1 (de) * | 2008-01-31 | 2009-08-06 | Osram Opto Semiconductors Gmbh | Hintergrundbeleuchtungseinheit für eine Hintergrundbeleuchtung eines Bildschirms und Bildschirmeinheit des Bildschirms |
| ATE530497T1 (de) | 2008-03-31 | 2011-11-15 | Sony Deutschland Gmbh | Verfahren zur herstellung einer membran mit konischer pore |
| EP2274446B1 (en) | 2008-03-31 | 2015-09-09 | Pacific Biosciences of California, Inc. | Two slow-step polymerase enzyme systems and methods |
| WO2009134469A1 (en) | 2008-04-29 | 2009-11-05 | Life Technologies | Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use |
| RU2487877C2 (ru) * | 2008-04-30 | 2013-07-20 | Иммьюноджен, Инк. | Высокоэффективные конъюгаты и гидрофильные сшивающие агенты (линкеры) |
| US8940142B2 (en) | 2008-05-05 | 2015-01-27 | The Regents Of The University Of California | Functionalized nanopipette biosensor |
| EP2307540B1 (en) | 2008-07-07 | 2017-04-19 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| JP5596684B2 (ja) * | 2008-08-12 | 2014-09-24 | エフ．ホフマン−ラ ロシュ アーゲー | プルーフリーディング・プライマー伸長 |
| US8921046B2 (en) | 2008-09-19 | 2014-12-30 | Pacific Biosciences Of California, Inc. | Nucleic acid sequence analysis |
| US20100148126A1 (en) | 2008-11-26 | 2010-06-17 | Board Of Regents, The University Of Texas System | Genomic sequencing using modified protein pores and ionic liquids |
| US20100227414A1 (en) | 2009-03-05 | 2010-09-09 | Trex Enterprises Corp. | Affinity capture mass spectroscopy with a porous silicon biosensor |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| EP2239053A1 (en) * | 2009-04-09 | 2010-10-13 | baseclick GmbH | Click chemistry on heterogeneous catalysts |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| US20130053252A1 (en) | 2009-09-25 | 2013-02-28 | President & Fellows Of Harvard College | Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides |
| EP2516386B1 (en) * | 2009-12-23 | 2015-04-22 | Life Technologies Corporation | Protein modification from the oxidation of clickable polyunsaturated fatty acid analogs |
| EP2360167A1 (en) * | 2010-02-03 | 2011-08-24 | Deutsches Krebsforschungszentrum | Post-synthetic modification of nucleic acids by inverse Diels-Alder reaction |
| US20110287414A1 (en) | 2010-02-08 | 2011-11-24 | Genia Technologies, Inc. | Systems and methods for identifying a portion of a molecule |
| US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| CA2995178C (en) | 2010-02-08 | 2023-09-12 | Genia Technologies, Inc. | Method and system for forming a nanopore in a lipid bilayer |
| US20110192723A1 (en) | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for manipulating a molecule in a nanopore |
| US20120052188A1 (en) | 2010-02-08 | 2012-03-01 | Genia Technologies, Inc. | Systems and methods for assembling a lipid bilayer on a substantially planar solid surface |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| US9234930B2 (en) | 2010-02-10 | 2016-01-12 | Lattice Semiconductor Corporation | Determination of physical connectivity status of devices based on electrical measurement |
| WO2011106456A2 (en) | 2010-02-23 | 2011-09-01 | University Of Washington | Artificial mycolic acid membranes |
| US8652779B2 (en) | 2010-04-09 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using charge blockade labels |
| EP3190193A1 (en) | 2010-07-14 | 2017-07-12 | The Curators Of The University Of Missouri | Nanopore-facilitated single molecule detection of nucleic acids |
| WO2012012612A2 (en) * | 2010-07-23 | 2012-01-26 | University Of Delaware | Tetrazine-trans-cyclooctene ligation for the rapid construction of radionuclide labeled probes |
| EP2652153B1 (en) * | 2010-12-17 | 2017-07-05 | The Trustees of Columbia University in the City of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
| WO2012088339A2 (en) | 2010-12-22 | 2012-06-28 | Genia Technologies, Inc. | Nanopore-based single dna molecule characterization using speed bumps |
| US8962242B2 (en) | 2011-01-24 | 2015-02-24 | Genia Technologies, Inc. | System for detecting electrical properties of a molecular complex |
| US9110478B2 (en) | 2011-01-27 | 2015-08-18 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
| WO2012109574A2 (en) | 2011-02-11 | 2012-08-16 | Nabsys, Inc. | Assay methods using dna binding proteins |
| US9150912B2 (en) * | 2011-05-06 | 2015-10-06 | Qiagen Gmbh | Oligonucleotides comprising a label associated through a linker |
| US9624539B2 (en) | 2011-05-23 | 2017-04-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using Raman and infrared spectroscopy detection |
| CN104011866B (zh) | 2011-07-27 | 2017-06-09 | 伊利诺伊大学评议会 | 用于生物分子表征的纳米孔传感器 |
| WO2013109970A1 (en) | 2012-01-20 | 2013-07-25 | Genia Technologies, Inc. | Nanopore based molecular detection and sequencing |
| US8541849B2 (en) | 2012-02-14 | 2013-09-24 | Genia Technologies, Inc. | Noise shielding techniques for ultra low current measurements in biochemical applications |
| EP2814983B1 (en) | 2012-02-16 | 2019-04-24 | Genia Technologies, Inc. | Methods for creating bilayers for use with nanopore sensors |
| US10246479B2 (en) | 2012-04-09 | 2019-04-02 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| GB2510719A (en) | 2012-06-15 | 2014-08-13 | Genia Technologies Inc | Chip set-up and high-accuracy nucleic acid sequencing |
| EP2864502B1 (en) | 2012-06-20 | 2019-10-23 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| WO2014081301A1 (en) | 2012-11-22 | 2014-05-30 | Tagworks Pharmaceuticals B.V. | Bio-orthogonal drug activation |
| WO2014117001A1 (en) | 2013-01-25 | 2014-07-31 | Memorial Sloan-Kettering Cancer Center | Tetrazines/trans-cyclooctenes in solid phase synthesis of labeled peptides |
| US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| CN105102627B (zh) | 2013-03-15 | 2018-10-19 | 纽约哥伦比亚大学理事会 | 用于检测样品中多种预定化合物的方法 |
| US10718011B2 (en) | 2014-02-12 | 2020-07-21 | The Trustees Of Columbia University In The City Of New York | Single molecule electronic multiplex SNP assay and PCR analysis |
| CA2943952A1 (en) | 2014-03-24 | 2015-10-01 | The Trustees Of Columbia University In The City Of New York | Chemical methods for producing tagged nucleotides |
| US20170101675A1 (en) | 2014-05-19 | 2017-04-13 | The Trustees Of Columbia University In The City Of New York | Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators |
| WO2016144973A1 (en) | 2015-03-09 | 2016-09-15 | The Trustees Of Columbia University In The City Of New York | Pore-forming protein conjugate compositions and methods |
| WO2016154215A1 (en) | 2015-03-23 | 2016-09-29 | The Trustees Of Columbia University In The City Of New York | Polymer tagged nucleotides for single molecule electronic snp assay |
| US11085076B2 (en) | 2015-09-28 | 2021-08-10 | The Trustees Of Columbia University In The City Of New York | Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis |
| US20180327828A1 (en) | 2015-11-18 | 2018-11-15 | The Trustees Of Columbia University In The City Of New York | Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators |
| WO2017176677A1 (en) | 2016-04-04 | 2017-10-12 | The Trustees Of Columbia University In The City Of New York | Flourescence energy transfer-based single molecule/ensemble dna sequencing by synthesis |
| WO2017176679A1 (en) | 2016-04-04 | 2017-10-12 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis with nucleotide analogues and raman detection |
| EP3463381A4 (en) | 2016-05-23 | 2020-01-01 | The Trustees of Columbia University in the City of New York | NUCLEOTIDE DERIVATIVES AND METHOD FOR USE THEREOF |
| CN110709089B (zh) | 2017-03-28 | 2024-12-06 | 哥伦比亚大学董事会 | 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物 |
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| CN106715453A (zh) | 2017-05-24 |
| CN106715453B (zh) | 2021-04-30 |
| WO2015148402A1 (en) | 2015-10-01 |
| JP2017515887A (ja) | 2017-06-15 |
| MA39774A (fr) | 2021-05-12 |
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