CA2917638C - RNA MULTIPLEX GUIDED GENOMIC ENGINEERING - Google Patents

RNA MULTIPLEX GUIDED GENOMIC ENGINEERING

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Publication number
CA2917638C
CA2917638C CA2917638A CA2917638A CA2917638C CA 2917638 C CA2917638 C CA 2917638C CA 2917638 A CA2917638 A CA 2917638A CA 2917638 A CA2917638 A CA 2917638A CA 2917638 C CA2917638 C CA 2917638C
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CA
Canada
Prior art keywords
dna
cell
rna
nucleic acid
guide rnas
Prior art date
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Active
Application number
CA2917638A
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English (en)
French (fr)
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CA2917638A1 (en
Inventor
George M. Church
James Dicarlo
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Harvard University
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Filing date
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Application filed by Harvard University filed Critical Harvard University
Priority to CA3192673A priority Critical patent/CA3192673A1/en
Publication of CA2917638A1 publication Critical patent/CA2917638A1/en
Application granted granted Critical
Publication of CA2917638C publication Critical patent/CA2917638C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CA2917638A 2013-07-09 2014-07-08 RNA MULTIPLEX GUIDED GENOMIC ENGINEERING Active CA2917638C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3192673A CA3192673A1 (en) 2013-07-09 2014-07-08 Multiplex rna-guided genome engineering

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361844168P 2013-07-09 2013-07-09
US61/844,168 2013-07-09
PCT/US2014/045691 WO2015006290A1 (en) 2013-07-09 2014-07-08 Multiplex rna-guided genome engineering

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3192673A Division CA3192673A1 (en) 2013-07-09 2014-07-08 Multiplex rna-guided genome engineering

Publications (2)

Publication Number Publication Date
CA2917638A1 CA2917638A1 (en) 2015-01-15
CA2917638C true CA2917638C (en) 2024-09-10

Family

ID=52280509

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2917638A Active CA2917638C (en) 2013-07-09 2014-07-08 RNA MULTIPLEX GUIDED GENOMIC ENGINEERING
CA3192673A Pending CA3192673A1 (en) 2013-07-09 2014-07-08 Multiplex rna-guided genome engineering

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3192673A Pending CA3192673A1 (en) 2013-07-09 2014-07-08 Multiplex rna-guided genome engineering

Country Status (12)

Country Link
US (2) US11459585B2 (enExample)
EP (2) EP3019616B1 (enExample)
JP (4) JP7120717B2 (enExample)
KR (5) KR20260047646A (enExample)
CN (2) CN116042726A (enExample)
AU (3) AU2014287393B2 (enExample)
CA (2) CA2917638C (enExample)
ES (1) ES2929143T3 (enExample)
IL (2) IL303973A (enExample)
RU (2) RU2706562C2 (enExample)
SG (3) SG11201600059QA (enExample)
WO (1) WO2015006290A1 (enExample)

Families Citing this family (130)

* Cited by examiner, † Cited by third party
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