CA2901588A1 - Compositions and methods for biological production of isoprene - Google Patents
Compositions and methods for biological production of isoprene Download PDFInfo
- Publication number
- CA2901588A1 CA2901588A1 CA2901588A CA2901588A CA2901588A1 CA 2901588 A1 CA2901588 A1 CA 2901588A1 CA 2901588 A CA2901588 A CA 2901588A CA 2901588 A CA2901588 A CA 2901588A CA 2901588 A1 CA2901588 A1 CA 2901588A1
- Authority
- CA
- Canada
- Prior art keywords
- isoprene
- nucleic acid
- methanotrophic
- exogenous nucleic
- naturally occurring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C11/00—Aliphatic unsaturated hydrocarbons
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- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
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- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03027—Isoprene synthase (4.2.3.27)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361774342P | 2013-03-07 | 2013-03-07 | |
| US61/774,342 | 2013-03-07 | ||
| US201461928333P | 2014-01-16 | 2014-01-16 | |
| US61/928,333 | 2014-01-16 | ||
| PCT/US2014/021258 WO2014138419A1 (en) | 2013-03-07 | 2014-03-06 | Compositions and methods for biological production of isoprene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2901588A1 true CA2901588A1 (en) | 2014-09-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2901588A Abandoned CA2901588A1 (en) | 2013-03-07 | 2014-03-06 | Compositions and methods for biological production of isoprene |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20160017374A1 (https=) |
| EP (1) | EP2964748A4 (https=) |
| JP (1) | JP2016508737A (https=) |
| KR (1) | KR20150125653A (https=) |
| CN (1) | CN105008514A (https=) |
| AU (1) | AU2014225631A1 (https=) |
| BR (1) | BR112015021003A2 (https=) |
| CA (1) | CA2901588A1 (https=) |
| RU (1) | RU2015131480A (https=) |
| WO (1) | WO2014138419A1 (https=) |
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| KR20150100666A (ko) * | 2012-12-27 | 2015-09-02 | 세키스이가가쿠 고교가부시키가이샤 | 재조합 세포, 및 이소프렌의 생산 방법 |
| CN105378077A (zh) | 2013-06-18 | 2016-03-02 | 凯利斯塔公司 | 用于使用乳酸脱氢酶转化体从c1化合物以生物方式产生乳酸的组合物和方法 |
| CN106029870A (zh) | 2014-01-16 | 2016-10-12 | 凯利斯塔公司 | 用于增强的氨基酸产生的微生物及相关方法 |
| US10443060B2 (en) | 2014-06-18 | 2019-10-15 | Calysta, Inc. | Nucleic acids and vectors for use with methanotrophic bacteria |
| JP2016059313A (ja) * | 2014-09-17 | 2016-04-25 | 積水化学工業株式会社 | 組換え細胞、並びに、イソプレン又は環式イソプレノイドの生産方法 |
| DE102015103608A1 (de) * | 2015-03-11 | 2016-09-15 | Basf Se | Verfahren zur mikrobiellen de novo Synthese von Terpenen |
| CA2984797A1 (en) | 2015-05-06 | 2016-11-10 | Trelys, Inc. | Compositions and methods for biological production of methionine |
| US10995316B2 (en) | 2015-05-13 | 2021-05-04 | Calysta, Inc. | Proline auxotrophs |
| US20180291401A1 (en) * | 2015-08-17 | 2018-10-11 | Invista North America S.A.R.L. | Methods, hosts, and reagents related thereto for production of unsaturated pentahydrocarbons, derivatives and intermediates thereof |
| US10533192B2 (en) | 2015-12-22 | 2020-01-14 | Nutech Ventures | Production of isoprene by methane-producing archaea |
| KR102546640B1 (ko) | 2016-01-26 | 2023-06-22 | 에스케이이노베이션 주식회사 | 재조합 호염성 메탄균주를 이용한 이소프렌의 제조방법 |
| JP2018014960A (ja) * | 2016-07-29 | 2018-02-01 | 横浜ゴム株式会社 | 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド |
| EP3505618A4 (en) * | 2016-10-28 | 2020-04-22 | Sekisui Chemical Co., Ltd. | RECOMBINANT CELL AND METHOD FOR PRODUCING ISOPRENE OR TERPEN |
| US11634733B2 (en) | 2017-06-30 | 2023-04-25 | Inv Nylon Chemicals Americas, Llc | Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof |
| US11162115B2 (en) | 2017-06-30 | 2021-11-02 | Inv Nylon Chemicals Americas, Llc | Methods, synthetic hosts and reagents for the biosynthesis of hydrocarbons |
| US11505809B2 (en) | 2017-09-28 | 2022-11-22 | Inv Nylon Chemicals Americas Llc | Organisms and biosynthetic processes for hydrocarbon synthesis |
| US11180707B2 (en) * | 2018-08-03 | 2021-11-23 | Faramaz Fred Faramarzi | System and method for duplicating flammable gas |
| CN109943493B (zh) * | 2019-04-17 | 2021-05-18 | 天津大学 | 实现通用酶催化功能多样性的突变体菌株及其构建方法 |
| JP7754406B2 (ja) * | 2021-03-26 | 2025-10-15 | 三菱ケミカル株式会社 | イソプレン産生能を有する遺伝子改変微生物およびこれを用いたイソプレン製造方法 |
| CN119736218A (zh) * | 2023-09-19 | 2025-04-01 | 西安卓虹超源生物科技有限公司 | 一种萜类化合物的生产及菌株 |
| KR20250113943A (ko) * | 2024-01-18 | 2025-07-28 | 한국생명공학연구원 | 메탄으로부터 이소프렌 생산능을 갖는 형질전환체 |
| WO2026010860A1 (en) | 2024-07-02 | 2026-01-08 | Bp Corporation North America Inc. | Recombinant microorganisms for isoprene production |
| WO2026022785A2 (en) * | 2024-07-26 | 2026-01-29 | Visolis, Inc. | Compositions for fuel blending made by a sustainable process, and sustainable fuel compositions therefrom |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6818424B2 (en) * | 2000-09-01 | 2004-11-16 | E. I. Du Pont De Nemours And Company | Production of cyclic terpenoids |
| AU9635901A (en) * | 2000-09-29 | 2002-04-08 | Cargill Inc | Isoprenoid production |
| US7947478B2 (en) * | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| CN105368764B (zh) * | 2007-12-13 | 2019-03-12 | 丹尼斯科美国公司 | 用于生产异戊二烯的组合物和方法 |
| CA2737223A1 (en) * | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| TW201412988A (zh) * | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| DE102012012913A1 (de) * | 2012-06-27 | 2014-01-02 | Beatrice Saier | Vorrichtung und Verfahren zur schaltbaren Förderung wenigstens eines Mediums |
| US20160002672A1 (en) * | 2012-12-21 | 2016-01-07 | Danisco Us Inc. | Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway |
| KR20150100666A (ko) * | 2012-12-27 | 2015-09-02 | 세키스이가가쿠 고교가부시키가이샤 | 재조합 세포, 및 이소프렌의 생산 방법 |
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| BR112015021003A2 (pt) | 2017-07-18 |
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| EP2964748A4 (en) | 2016-11-02 |
| KR20150125653A (ko) | 2015-11-09 |
| US20160017374A1 (en) | 2016-01-21 |
| RU2015131480A (ru) | 2017-04-12 |
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