CA2874492C - Nano46 genes and methods to predict breast cancer outcome - Google Patents
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| JP7352937B2 (ja) * | 2019-07-19 | 2023-09-29 | 公立大学法人福島県立医科大学 | 乳癌のサブタイプを鑑別又は分類するための鑑別マーカー遺伝子セット、方法およびキット |
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| EP3945136A1 (en) * | 2020-07-28 | 2022-02-02 | Hospital Clínic de Barcelona | In vitro method for the prognosis of patients suffering from her2-positive breast cancer |
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| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| JP2024543736A (ja) * | 2021-12-20 | 2024-11-22 | リヴィール ゲノミクス エセ．エレ | ＨＥＲ２陽性乳癌を患う患者の予後診断を行うｉｎ ｖｉｔｒｏ方法の開発及び検証 |
| CN117965734B (zh) * | 2024-02-02 | 2024-09-24 | 奥明星程(杭州)生物科技有限公司 | 一种用于检测硬纤维瘤的基因标志物、试剂盒、检测方法及应用 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| JP2001524926A (ja) | 1991-09-18 | 2001-12-04 | アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ | オリゴマーの雑多ライブラリーの集合体を合成する方法 |
| ATE262374T1 (de) | 1991-11-22 | 2004-04-15 | Affymetrix Inc | Kombinatorische strategien für polymersynthese |
| US5384261A (en) | 1991-11-22 | 1995-01-24 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis using mechanically directed flow paths |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| EP0880598A4 (en) | 1996-01-23 | 2005-02-23 | Affymetrix Inc | RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM |
| ATE291097T1 (de) | 1997-10-31 | 2005-04-15 | Affymetrix Inc A Delaware Corp | Expressionsprofile in adulten und fötalen organen |
| US6020135A (en) | 1998-03-27 | 2000-02-01 | Affymetrix, Inc. | P53-regulated genes |
| US6750015B2 (en) * | 2000-06-28 | 2004-06-15 | Kathryn B. Horwitz | Progesterone receptor-regulated gene expression and methods related thereto |
| US20020168639A1 (en) * | 2000-09-22 | 2002-11-14 | Muraca Patrick J. | Profile array substrates |
| JP4680898B2 (ja) * | 2003-06-24 | 2011-05-11 | ジェノミック ヘルス， インコーポレイテッド | 癌再発の可能性の予測 |
| US20050071087A1 (en) * | 2003-09-29 | 2005-03-31 | Anderson Glenda G. | Systems and methods for detecting biological features |
| WO2006010150A2 (en) | 2004-07-15 | 2006-01-26 | University Of Utah Research Foundation | Housekeeping genes and methods for identifying the same |
| CN101061480A (zh) * | 2004-09-22 | 2007-10-24 | 三路影像公司 | 用来分析和优化用于癌症诊断的标记候选物的方法和计算机程序产品 |
| WO2007061876A2 (en) | 2005-11-23 | 2007-05-31 | University Of Utah Research Foundation | Methods and compositions involving intrinsic genes |
| CA2635215C (en) | 2005-12-23 | 2016-08-30 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
| CA2640385C (en) | 2005-12-23 | 2014-07-15 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| WO2008124847A2 (en) | 2007-04-10 | 2008-10-16 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| WO2008150512A2 (en) * | 2007-06-04 | 2008-12-11 | University Of Louisville Research Foundation, Inc. | Methods for identifying an increased likelihood of recurrence of breast cancer |
| EP2195467B1 (en) * | 2007-09-06 | 2016-12-14 | bioTheranostics, Inc. | Tumor grading and cancer prognosis in breast cancer |
| HRP20140140T1 (en) * | 2008-05-30 | 2014-05-23 | The University Of North Carolina At Chapel Hill | Gene expression profiles to predict breast cancer outcomes |
| WO2010019826A1 (en) | 2008-08-14 | 2010-02-18 | Nanostring Technologies, Inc | Stable nanoreporters |
| CN102971435B (zh) * | 2010-03-31 | 2016-05-11 | 斯维丹诊断有限责任公司 | 用于内分泌治疗下的乳腺癌复发预测的方法 |
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