CA2869472C - Preparation d'echantillon pour cytometrie de flux - Google Patents
Preparation d'echantillon pour cytometrie de flux Download PDFInfo
- Publication number
- CA2869472C CA2869472C CA2869472A CA2869472A CA2869472C CA 2869472 C CA2869472 C CA 2869472C CA 2869472 A CA2869472 A CA 2869472A CA 2869472 A CA2869472 A CA 2869472A CA 2869472 C CA2869472 C CA 2869472C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- background signal
- reducing
- molecule
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000684 flow cytometry Methods 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title description 12
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 104
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 104
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 104
- 238000000034 method Methods 0.000 claims abstract description 87
- 244000005700 microbiome Species 0.000 claims abstract description 83
- 239000013618 particulate matter Substances 0.000 claims abstract description 35
- 239000011347 resin Substances 0.000 claims abstract description 32
- 229920005989 resin Polymers 0.000 claims abstract description 32
- 239000000523 sample Substances 0.000 claims description 179
- 238000001514 detection method Methods 0.000 claims description 27
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 21
- 239000012466 permeate Substances 0.000 claims description 21
- 230000002829 reductive effect Effects 0.000 claims description 19
- 239000002245 particle Substances 0.000 claims description 16
- 235000013305 food Nutrition 0.000 claims description 15
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 10
- 150000001555 benzenes Chemical class 0.000 claims description 9
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 8
- -1 alkyl amide Chemical class 0.000 claims description 8
- 238000010791 quenching Methods 0.000 claims description 8
- LISFMEBWQUVKPJ-UHFFFAOYSA-N quinolin-2-ol Chemical group C1=CC=C2NC(=O)C=CC2=C1 LISFMEBWQUVKPJ-UHFFFAOYSA-N 0.000 claims description 8
- 229910052717 sulfur Inorganic materials 0.000 claims description 8
- 239000011593 sulfur Substances 0.000 claims description 8
- 239000012472 biological sample Substances 0.000 claims description 6
- 230000007613 environmental effect Effects 0.000 claims description 6
- 230000035699 permeability Effects 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical group OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims description 5
- 125000000217 alkyl group Chemical group 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 125000004432 carbon atom Chemical group C* 0.000 claims description 5
- 229910052739 hydrogen Inorganic materials 0.000 claims description 5
- 239000001257 hydrogen Substances 0.000 claims description 5
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims description 5
- 230000000171 quenching effect Effects 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 5
- 239000002537 cosmetic Substances 0.000 claims description 4
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 238000010186 staining Methods 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 44
- 238000003556 assay Methods 0.000 description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 23
- 239000011159 matrix material Substances 0.000 description 22
- 239000001974 tryptic soy broth Substances 0.000 description 22
- 108010050327 trypticase-soy broth Proteins 0.000 description 22
- 239000000203 mixture Substances 0.000 description 21
- 230000027455 binding Effects 0.000 description 13
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 13
- 244000052769 pathogen Species 0.000 description 12
- 239000000975 dye Substances 0.000 description 11
- 239000000980 acid dye Substances 0.000 description 10
- 239000002028 Biomass Substances 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- 125000000623 heterocyclic group Chemical group 0.000 description 6
- 235000013372 meat Nutrition 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 230000002452 interceptive effect Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 230000009871 nonspecific binding Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 239000013076 target substance Substances 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 125000001624 naphthyl group Chemical group 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 125000004433 nitrogen atom Chemical group N* 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000011533 pre-incubation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- CHADEQDQBURGHL-UHFFFAOYSA-N (6'-acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(OC(C)=O)C=C1OC1=CC(OC(=O)C)=CC=C21 CHADEQDQBURGHL-UHFFFAOYSA-N 0.000 description 1
- CVOFKRWYWCSDMA-UHFFFAOYSA-N 2-chloro-n-(2,6-diethylphenyl)-n-(methoxymethyl)acetamide;2,6-dinitro-n,n-dipropyl-4-(trifluoromethyl)aniline Chemical compound CCC1=CC=CC(CC)=C1N(COC)C(=O)CCl.CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O CVOFKRWYWCSDMA-UHFFFAOYSA-N 0.000 description 1
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 1
- 241000223936 Cryptosporidium parvum Species 0.000 description 1
- 241000016605 Cyclospora cayetanensis Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 244000078673 foodborn pathogen Species 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000011090 industrial biotechnology method and process Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Plural Heterocyclic Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La présente invention concerne des procédés et des réactifs qui permettent d'identifier et d'analyser au moins un micro-organisme (par exemple des bactéries) dans un échantillon, et de réduire l'intensité de signal de bruit de fond obtenue lors de l'analyse de l'échantillon par cytométrie de flux. L'échantillon est préparé par la combinaison de l'échantillon avec une molécule réduisant le signal de bruit de fond ou avec un marqueur d'acide nucléique lié de façon covalente à un extincteur de luminescence. Une partie de la matière particulaire dans l'échantillon peut être facultativement éliminée avec une résine avant le marquage par un marqueur d'acide nucléique.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261620823P | 2012-04-05 | 2012-04-05 | |
| US61/620,823 | 2012-04-05 | ||
| PCT/US2013/035291 WO2013152203A1 (fr) | 2012-04-05 | 2013-04-04 | Préparation d'échantillon pour cytométrie de flux |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2869472A1 CA2869472A1 (fr) | 2013-10-10 |
| CA2869472C true CA2869472C (fr) | 2017-07-11 |
Family
ID=49301050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2869472A Active CA2869472C (fr) | 2012-04-05 | 2013-04-04 | Preparation d'echantillon pour cytometrie de flux |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2834367A4 (fr) |
| JP (1) | JP6401146B2 (fr) |
| CN (1) | CN104379760B (fr) |
| AU (1) | AU2013243378B2 (fr) |
| CA (1) | CA2869472C (fr) |
| IN (1) | IN2014DN08957A (fr) |
| WO (1) | WO2013152203A1 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| WO2008014485A2 (fr) | 2006-07-28 | 2008-01-31 | California Institute Of Technology | Ensembles de pcr-q multiplex |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US9645057B2 (en) | 2012-04-05 | 2017-05-09 | Becton, Dickiinson and Company | Method for improving analysis of microorganisms in complex matrices |
| JP6353518B2 (ja) * | 2013-03-13 | 2018-07-04 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | 複合マトリックス中の微生物の分析を向上させるための方法 |
| CN104677809B (zh) * | 2013-11-29 | 2018-01-02 | 中国人民解放军第二军医大学 | 隐球菌活力检测试剂盒及检测方法 |
| US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
| US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
| WO2017155858A1 (fr) * | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Identification de séquence d'acide nucléique à l'aide d'une extension de base unique cyclique en phase solide |
| CN107643238A (zh) * | 2017-09-19 | 2018-01-30 | 中山大学附属第医院 | 一种定量检测血液循环微粒浓度的方法 |
| EP3830547B1 (fr) * | 2018-08-01 | 2023-11-29 | Sartorius BioAnalytical Instruments, Inc. | Procédés, trousses et compositions pour une évaluation par cytométrie de particules de dimension de virus à l'aide de multiples colorants fluorescentes |
| CN113924041A (zh) | 2019-03-14 | 2022-01-11 | 因斯利克萨公司 | 基于时间门控的荧光检测的方法和系统 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5436134A (en) * | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| DE69615818T2 (de) * | 1995-01-16 | 2002-06-06 | Erkki Soini | Ein biospezifisches multiparametrisches testverfahren |
| US20030013849A1 (en) * | 1999-10-29 | 2003-01-16 | Ward William W. | Renilla reniformis green fluorescent protein |
| RU2172512C1 (ru) * | 2000-02-01 | 2001-08-20 | Закрытое акционерное общество Научно-производственное объединение "ФоМос" | Цветной спектрозональный галогенсеребряный фотографический материал |
| US6323337B1 (en) * | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| CN1545557A (zh) * | 2000-11-17 | 2004-11-10 | �ݰ�Ī�﹫˾ | 应用5,10,15,20-四(羧苯基)卟吩检测细胞及组织样本中癌前期状态的组合物和方法 |
| US7205100B2 (en) * | 2002-10-10 | 2007-04-17 | Advanced Analytical Technologies, Inc. | Methods for reducing background fluorescence |
| US8481302B2 (en) * | 2008-11-03 | 2013-07-09 | General Electric Company | Total bacteria monitoring system |
| GB201005939D0 (en) * | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
| US20130095496A1 (en) * | 2010-06-21 | 2013-04-18 | Siemens Healthcare Diagnostics Inc. | Soluble Quencher to reduce Background in qPCR assays |
-
2013
- 2013-04-04 AU AU2013243378A patent/AU2013243378B2/en active Active
- 2013-04-04 CA CA2869472A patent/CA2869472C/fr active Active
- 2013-04-04 CN CN201380029083.0A patent/CN104379760B/zh active Active
- 2013-04-04 WO PCT/US2013/035291 patent/WO2013152203A1/fr active Application Filing
- 2013-04-04 JP JP2015504727A patent/JP6401146B2/ja active Active
- 2013-04-04 EP EP13773119.6A patent/EP2834367A4/fr not_active Withdrawn
- 2013-04-04 IN IN8957DEN2014 patent/IN2014DN08957A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2869472A1 (fr) | 2013-10-10 |
| AU2013243378A1 (en) | 2014-10-30 |
| JP6401146B2 (ja) | 2018-10-03 |
| EP2834367A4 (fr) | 2016-03-23 |
| CN104379760A (zh) | 2015-02-25 |
| EP2834367A1 (fr) | 2015-02-11 |
| CN104379760B (zh) | 2018-03-16 |
| WO2013152203A1 (fr) | 2013-10-10 |
| IN2014DN08957A (fr) | 2015-05-22 |
| AU2013243378B2 (en) | 2019-04-11 |
| JP2015514403A (ja) | 2015-05-21 |
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