AU2013243378B2 - Sample preparation for flow cytometry - Google Patents

Sample preparation for flow cytometry Download PDF

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Publication number
AU2013243378B2
AU2013243378B2 AU2013243378A AU2013243378A AU2013243378B2 AU 2013243378 B2 AU2013243378 B2 AU 2013243378B2 AU 2013243378 A AU2013243378 A AU 2013243378A AU 2013243378 A AU2013243378 A AU 2013243378A AU 2013243378 B2 AU2013243378 B2 AU 2013243378B2
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AU
Australia
Prior art keywords
sample
nucleic acid
moiety
background signal
acid stain
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Active
Application number
AU2013243378A
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English (en)
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AU2013243378A1 (en
Inventor
Kenneth Anthony Kopher
Xiao Li
John D. Mantlo
William Alfred Pope
Song Shi
Axel A. Yup
Yongqiang Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
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Publication date
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Publication of AU2013243378A1 publication Critical patent/AU2013243378A1/en
Application granted granted Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
AU2013243378A 2012-04-05 2013-04-04 Sample preparation for flow cytometry Active AU2013243378B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261620823P 2012-04-05 2012-04-05
US61/620,823 2012-04-05
PCT/US2013/035291 WO2013152203A1 (fr) 2012-04-05 2013-04-04 Préparation d'échantillon pour cytométrie de flux

Publications (2)

Publication Number Publication Date
AU2013243378A1 AU2013243378A1 (en) 2014-10-30
AU2013243378B2 true AU2013243378B2 (en) 2019-04-11

Family

ID=49301050

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013243378A Active AU2013243378B2 (en) 2012-04-05 2013-04-04 Sample preparation for flow cytometry

Country Status (7)

Country Link
EP (1) EP2834367A4 (fr)
JP (1) JP6401146B2 (fr)
CN (1) CN104379760B (fr)
AU (1) AU2013243378B2 (fr)
CA (1) CA2869472C (fr)
IN (1) IN2014DN08957A (fr)
WO (1) WO2013152203A1 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
WO2008014485A2 (fr) 2006-07-28 2008-01-31 California Institute Of Technology Ensembles de pcr-q multiplex
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9645057B2 (en) 2012-04-05 2017-05-09 Becton, Dickiinson and Company Method for improving analysis of microorganisms in complex matrices
EP2971050B1 (fr) * 2013-03-13 2018-12-19 Becton, Dickinson and Company Procédé permettant une meilleure analyse des microorganismes présents dans des matrices complexes
CN104677809B (zh) * 2013-11-29 2018-01-02 中国人民解放军第二军医大学 隐球菌活力检测试剂盒及检测方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (fr) * 2016-03-07 2017-09-14 Insilixa, Inc. Identification de séquence d'acide nucléique à l'aide d'une extension de base unique cyclique en phase solide
CN107643238A (zh) * 2017-09-19 2018-01-30 中山大学附属第医院 一种定量检测血液循环微粒浓度的方法
ES2967515T3 (es) * 2018-08-01 2024-04-30 Sartorius Bioanalytical Instr Inc Métodos, kits y composiciones de tinción para la evaluación de citometría de flujo de partículas del tamaño de un virus no asociadas utilizando múltiples colorantes fluorogénicos
JP2022525322A (ja) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム

Citations (2)

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Publication number Priority date Publication date Assignee Title
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method
EP1193547A1 (fr) * 2000-02-01 2002-04-03 Zakrytoe Aktsionernoe Obschestvo Ordena Trudovogo Krasnogo Znameni Nauchno-Proizvodstvennoe Obiedinenie "Fomos" (ZAO NPO... Materiau photographique couleur a bandes spectrales a base d'halogenures d'argent

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Publication number Priority date Publication date Assignee Title
US20030013849A1 (en) * 1999-10-29 2003-01-16 Ward William W. Renilla reniformis green fluorescent protein
US6323337B1 (en) * 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
DK2372361T3 (da) * 2000-11-17 2014-11-10 Bioaffinity Technologies Inc Sammensætninger og fremgangsmåder til påvisning af præcancerøse tilstande i celle- og vævsprøver under anvendelse af 5,10,15,20-tetrakis(carboxyphenyl)porphin
US7205100B2 (en) * 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
EP2582838B1 (fr) * 2010-06-21 2014-07-16 Siemens Healthcare Diagnostics Inc. Désactivateur soluble pour réduire le bruit de fond dans les dosages de pcr quantitative

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method
EP1193547A1 (fr) * 2000-02-01 2002-04-03 Zakrytoe Aktsionernoe Obschestvo Ordena Trudovogo Krasnogo Znameni Nauchno-Proizvodstvennoe Obiedinenie "Fomos" (ZAO NPO... Materiau photographique couleur a bandes spectrales a base d'halogenures d'argent

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Hofer, L. J. E. et al, "The fluorescence of cyanine and related dyes in the monomeric state", Journal of the American Chemical Society, 1950, Vol. 72, pp 203-209 *
JUNG KYU CHOI ET AL, "3,3'-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, (2011-11-16), vol. 12, no. 12, pages 8052 - 8062 *

Also Published As

Publication number Publication date
AU2013243378A1 (en) 2014-10-30
JP6401146B2 (ja) 2018-10-03
EP2834367A1 (fr) 2015-02-11
CN104379760B (zh) 2018-03-16
EP2834367A4 (fr) 2016-03-23
CA2869472C (fr) 2017-07-11
JP2015514403A (ja) 2015-05-21
CA2869472A1 (fr) 2013-10-10
IN2014DN08957A (fr) 2015-05-22
CN104379760A (zh) 2015-02-25
WO2013152203A1 (fr) 2013-10-10

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