CA2861959A1 - Biomolecule isolation - Google Patents
Biomolecule isolation Download PDFInfo
- Publication number
- CA2861959A1 CA2861959A1 CA2861959A CA2861959A CA2861959A1 CA 2861959 A1 CA2861959 A1 CA 2861959A1 CA 2861959 A CA2861959 A CA 2861959A CA 2861959 A CA2861959 A CA 2861959A CA 2861959 A1 CA2861959 A1 CA 2861959A1
- Authority
- CA
- Canada
- Prior art keywords
- liquid
- conduit
- magnetic particles
- flowing
- trapping site
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000002955 isolation Methods 0.000 title description 14
- 239000007788 liquid Substances 0.000 claims abstract description 179
- 238000000034 method Methods 0.000 claims abstract description 73
- 239000006249 magnetic particle Substances 0.000 claims abstract description 62
- 230000005291 magnetic effect Effects 0.000 claims description 87
- 239000012530 fluid Substances 0.000 claims description 47
- 238000010828 elution Methods 0.000 claims description 46
- 238000004140 cleaning Methods 0.000 claims description 44
- 239000002131 composite material Substances 0.000 claims description 17
- 230000003287 optical effect Effects 0.000 claims description 8
- 230000001483 mobilizing effect Effects 0.000 claims description 6
- 230000003100 immobilizing effect Effects 0.000 claims description 5
- 230000027455 binding Effects 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims 1
- 239000011324 bead Substances 0.000 description 177
- 108020004414 DNA Proteins 0.000 description 100
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 100
- 101150047834 SNAI2 gene Proteins 0.000 description 78
- 239000000523 sample Substances 0.000 description 78
- 239000012149 elution buffer Substances 0.000 description 59
- 230000005298 paramagnetic effect Effects 0.000 description 56
- 238000000746 purification Methods 0.000 description 56
- 239000003921 oil Substances 0.000 description 46
- 239000000047 product Substances 0.000 description 45
- 239000000203 mixture Substances 0.000 description 40
- 210000004027 cell Anatomy 0.000 description 38
- 239000000243 solution Substances 0.000 description 34
- 239000003570 air Substances 0.000 description 32
- 239000008188 pellet Substances 0.000 description 28
- 241000237858 Gastropoda Species 0.000 description 27
- 238000006243 chemical reaction Methods 0.000 description 27
- 238000004458 analytical method Methods 0.000 description 23
- 239000000872 buffer Substances 0.000 description 21
- 238000002360 preparation method Methods 0.000 description 21
- 238000011084 recovery Methods 0.000 description 20
- 238000013459 approach Methods 0.000 description 19
- 238000012545 processing Methods 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 14
- 239000012634 fragment Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 230000008569 process Effects 0.000 description 13
- 238000005406 washing Methods 0.000 description 12
- 239000013641 positive control Substances 0.000 description 11
- 238000003753 real-time PCR Methods 0.000 description 11
- 101710163270 Nuclease Proteins 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 239000011248 coating agent Substances 0.000 description 10
- 238000000576 coating method Methods 0.000 description 10
- 239000000356 contaminant Substances 0.000 description 10
- 150000007523 nucleic acids Chemical class 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 9
- 108091093088 Amplicon Proteins 0.000 description 8
- 238000007481 next generation sequencing Methods 0.000 description 8
- 239000013615 primer Substances 0.000 description 8
- 239000002987 primer (paints) Substances 0.000 description 8
- 239000012472 biological sample Substances 0.000 description 7
- 108020004707 nucleic acids Proteins 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
- 238000012163 sequencing technique Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 238000001502 gel electrophoresis Methods 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 238000011109 contamination Methods 0.000 description 5
- 238000005202 decontamination Methods 0.000 description 5
- 230000003588 decontaminative effect Effects 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 230000004568 DNA-binding Effects 0.000 description 4
- 101000756632 Homo sapiens Actin, cytoplasmic 1 Proteins 0.000 description 4
- 239000012148 binding buffer Substances 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- 102100022900 Actin, cytoplasmic 1 Human genes 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000539 dimer Substances 0.000 description 3
- -1 etc) Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000001821 nucleic acid purification Methods 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- GUAHPAJOXVYFON-ZETCQYMHSA-N (8S)-8-amino-7-oxononanoic acid zwitterion Chemical compound C[C@H](N)C(=O)CCCCCC(O)=O GUAHPAJOXVYFON-ZETCQYMHSA-N 0.000 description 2
- 108091032955 Bacterial small RNA Proteins 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 102000008579 Transposases Human genes 0.000 description 2
- 108010020764 Transposases Proteins 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 239000011368 organic material Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000005382 thermal cycling Methods 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- MCTWTZJPVLRJOU-UHFFFAOYSA-N 1-methyl-1H-imidazole Chemical compound CN1C=CN=C1 MCTWTZJPVLRJOU-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 208000007660 Residual Neoplasm Diseases 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000006642 detritylation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007847 digital PCR Methods 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 230000005672 electromagnetic field Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 238000010448 genetic screening Methods 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000005661 hydrophobic surface Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 239000010702 perfluoropolyether Substances 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- TXEYQDLBPFQVAA-UHFFFAOYSA-N tetrafluoromethane Chemical compound FC(F)(F)F TXEYQDLBPFQVAA-UHFFFAOYSA-N 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
- B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Fluid Mechanics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261590499P | 2012-01-25 | 2012-01-25 | |
| US61/590,499 | 2012-01-25 | ||
| PCT/IB2013/000478 WO2013111016A2 (en) | 2012-01-25 | 2013-01-25 | Biomolecule isolation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2861959A1 true CA2861959A1 (en) | 2013-08-01 |
Family
ID=48326342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2861959A Abandoned CA2861959A1 (en) | 2012-01-25 | 2013-01-25 | Biomolecule isolation |
Country Status (10)
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5893620B2 (ja) | 2010-07-22 | 2016-03-23 | ジェンセル バイオシステムズ リミテッド | 合成液体セル |
| ES2642862T3 (es) | 2012-01-25 | 2017-11-20 | Gencell Biosystems Limited | Aislamiento de biomoléculas |
| AU2013350823B2 (en) | 2012-11-27 | 2017-11-16 | Gencell Biosystems Ltd. | Handling liquid samples |
| US10143989B2 (en) | 2013-05-07 | 2018-12-04 | Gencell Biosystems Ltd. | Stabilisation features |
| CN105339497A (zh) | 2013-06-18 | 2016-02-17 | 基因细胞生物系统有限公司 | 生物分子分离和热加工 |
| US10722879B2 (en) | 2013-11-25 | 2020-07-28 | Gencell Biosystems Ltd. | Magnetic separation |
| GB201321433D0 (en) * | 2013-12-04 | 2014-01-15 | Isis Innovation | Controling fluid micro compartments |
| EP3105326A4 (en) | 2014-02-10 | 2018-01-10 | Gencell Biosystems Limited | Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same |
| EP3177393A1 (en) | 2014-08-04 | 2017-06-14 | Gencell Biosystems Limited | Benchtop nucleic acid library preparation device and methods for using the same |
| EP3177917B1 (en) | 2014-08-04 | 2019-10-02 | Gencell Biosystems Limited | Genomic analysis device and methods of use |
| WO2016020837A1 (en) | 2014-08-04 | 2016-02-11 | Gencell Biosystems Ltd. | Triphasic fluid handling |
| CN107249746B (zh) * | 2014-11-21 | 2020-05-15 | 澳康姆生物实验室公司 | 用于收集核酸样本的系统和方法 |
| EP3227461A1 (en) * | 2014-12-05 | 2017-10-11 | Amyris, Inc. | High-throughput sequencing of polynucleotides |
| PL413551A1 (pl) * | 2015-08-18 | 2017-02-27 | Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością | Sposób i system do izolacji kwasów nukleinowych |
| US20170058277A1 (en) | 2015-08-26 | 2017-03-02 | Gencell Biosystems Ltd. | Composite liquid cell (clc) supports, and methods of making and using the same |
| JP6884562B2 (ja) * | 2016-11-30 | 2021-06-09 | シスメックス株式会社 | 検体処理方法および検体処理装置 |
| CN111849696A (zh) * | 2019-04-25 | 2020-10-30 | 蒋霆辉 | 一种动静脉血管循环异常细胞捕获装置 |
| CN115873691B (zh) * | 2023-02-24 | 2023-05-16 | 北京凡知医学科技有限公司 | 一种微流控芯片和核酸提取纯化方法及装置 |
Family Cites Families (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1575805A (en) * | 1976-03-12 | 1980-10-01 | Technicon Instr | Automatic diagnostic apparatus |
| AU3182289A (en) * | 1988-02-10 | 1989-09-22 | Nygene Corporation | Process for producing biochemicals |
| DE69228247T2 (de) | 1991-08-10 | 1999-07-08 | Medical Research Council, London | Behandlung von Zellpopulationen |
| US5505877A (en) | 1991-10-15 | 1996-04-09 | Krivohlavek; Dennis | Making multiple phase emulsion or gel |
| EP2258846A3 (en) | 1997-07-07 | 2012-03-21 | Medical Research Council | A method for increasing the concentration of a nucleic acid molecule |
| US5902227A (en) | 1997-07-17 | 1999-05-11 | Intevep, S.A. | Multiple emulsion and method for preparing same |
| US5980936A (en) | 1997-08-07 | 1999-11-09 | Alliance Pharmaceutical Corp. | Multiple emulsions comprising a hydrophobic continuous phase |
| EP1053784A3 (en) | 1999-05-21 | 2001-05-23 | Bruker Daltonik GmbH | Processing samples in solutions having defined small contact area with support |
| CA2379503A1 (en) * | 1999-08-20 | 2001-03-01 | Promega Corporation | Simultaneous isolation and quantitation of dna |
| DE10111520B4 (de) | 2001-03-09 | 2004-01-15 | Chemagen Biopolymer-Technologie Aktiengesellschaft | Verfahren zur Aufreinigung von Biomolekülen mit Hilfe magnetischer Partikel |
| AU2003219870A1 (en) | 2002-02-22 | 2003-09-09 | Biodot, Inc. | Method and apparatus for dispersing reagent droplets below a fluid surface using non-contact dispensing |
| EP2278338B1 (en) | 2002-05-09 | 2020-08-26 | The University of Chicago | Device and method for pressure-driven plug transport and reaction |
| US7901939B2 (en) | 2002-05-09 | 2011-03-08 | University Of Chicago | Method for performing crystallization and reactions in pressure-driven fluid plugs |
| AU2004209416B2 (en) | 2003-01-29 | 2007-08-02 | 454 Life Sciences Corporation | Double ended sequencing |
| GB0315438D0 (en) | 2003-07-02 | 2003-08-06 | Univ Manchester | Analysis of mixed cell populations |
| JP4417332B2 (ja) * | 2004-01-15 | 2010-02-17 | 独立行政法人科学技術振興機構 | 化学分析装置及び化学分析方法 |
| KR100552706B1 (ko) | 2004-03-12 | 2006-02-20 | 삼성전자주식회사 | 핵산 증폭 방법 및 장치 |
| JP2006010332A (ja) * | 2004-06-22 | 2006-01-12 | Eiichi Tamiya | 微小容量の試料溶液を形成する方法 |
| CA2599683A1 (en) | 2005-03-04 | 2006-09-14 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
| WO2007024800A2 (en) | 2005-08-22 | 2007-03-01 | Applera Corporation | Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other |
| US8637317B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Method of washing beads |
| US20140193807A1 (en) | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
| US8613889B2 (en) | 2006-04-13 | 2013-12-24 | Advanced Liquid Logic, Inc. | Droplet-based washing |
| US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
| JP5054096B2 (ja) * | 2006-04-18 | 2012-10-24 | アドヴァンスト リキッド ロジック インコーポレイテッド | 液滴に基づく生化学 |
| WO2009140671A2 (en) | 2008-05-16 | 2009-11-19 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods for manipulating beads |
| US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
| US8685754B2 (en) | 2006-04-18 | 2014-04-01 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods for immunoassays and washing |
| CA2653321A1 (en) * | 2006-05-26 | 2007-12-06 | Althea Technologies, Inc. | Biochemical analysis of partitioned cells |
| US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| CN102014871A (zh) | 2007-03-28 | 2011-04-13 | 哈佛大学 | 乳液及其形成技术 |
| US20090023189A1 (en) | 2007-05-18 | 2009-01-22 | Applera Corporation | Apparatus and methods for preparation of subtantially uniform emulsions containing a particle |
| WO2009018348A1 (en) * | 2007-07-30 | 2009-02-05 | Cornell Research Foundation, Inc. | Microchannel detection device and use thereof |
| WO2009061372A1 (en) | 2007-11-02 | 2009-05-14 | President And Fellows Of Harvard College | Systems and methods for creating multi-phase entities, including particles and/or fluids |
| US8815576B2 (en) | 2007-12-27 | 2014-08-26 | Lawrence Livermore National Security, Llc. | Chip-based sequencing nucleic acids |
| US8067176B2 (en) * | 2008-01-25 | 2011-11-29 | Shimadzu Corporation | Microchemistry reaction method |
| EP2250285A2 (en) * | 2008-02-15 | 2010-11-17 | Life Technologies Corporation | Methods and kits for extraction of dna |
| US8852952B2 (en) * | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
| US8093064B2 (en) | 2008-05-15 | 2012-01-10 | The Regents Of The University Of California | Method for using magnetic particles in droplet microfluidics |
| WO2010009365A1 (en) | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| WO2010104604A1 (en) | 2009-03-13 | 2010-09-16 | President And Fellows Of Harvard College | Method for the controlled creation of emulsions, including multiple emulsions |
| WO2010121307A1 (en) | 2009-04-21 | 2010-10-28 | The University Of Queensland | Complex emulsions |
| WO2011079176A2 (en) | 2009-12-23 | 2011-06-30 | Raindance Technologies, Inc. | Microfluidic systems and methods for reducing the exchange of molecules between droplets |
| JP5573335B2 (ja) | 2010-04-28 | 2014-08-20 | 株式会社島津製作所 | 磁性体粒子操作デバイス及び磁性体粒子操作方法 |
| JP5893620B2 (ja) | 2010-07-22 | 2016-03-23 | ジェンセル バイオシステムズ リミテッド | 合成液体セル |
| WO2012018623A2 (en) | 2010-07-26 | 2012-02-09 | The Johns Hopkins University | Self-sustained fluidic droplet cassette and system for biochemical assays |
| US9266104B2 (en) | 2011-02-11 | 2016-02-23 | Raindance Technologies, Inc. | Thermocycling device for nucleic acid amplification and methods of use |
| ES2642862T3 (es) | 2012-01-25 | 2017-11-20 | Gencell Biosystems Limited | Aislamiento de biomoléculas |
| EP2864495A4 (en) | 2012-06-20 | 2016-06-08 | Bio Rad Laboratories | STABILIZED DROPLETS FOR CALIBRATING AND TESTING |
| EP2703497B1 (en) | 2012-08-31 | 2016-06-22 | Roche Diagniostics GmbH | Microfluidic chip, device and system for the generation of aqueous droplets in emulsion oil for nucleic acid amplification |
| WO2014039587A1 (en) | 2012-09-05 | 2014-03-13 | Bio-Rad Laboratories, Inc. | Systems and methods for stabilizing droplets |
| US9840734B2 (en) | 2012-10-22 | 2017-12-12 | Raindance Technologies, Inc. | Methods for analyzing DNA |
| AU2013350823B2 (en) | 2012-11-27 | 2017-11-16 | Gencell Biosystems Ltd. | Handling liquid samples |
| WO2014093714A1 (en) | 2012-12-14 | 2014-06-19 | Bio-Rad Laboratories, Inc. | Methods and compositions for using oils for analysis and detection of molecules |
| US10143989B2 (en) | 2013-05-07 | 2018-12-04 | Gencell Biosystems Ltd. | Stabilisation features |
| CN105339497A (zh) | 2013-06-18 | 2016-02-17 | 基因细胞生物系统有限公司 | 生物分子分离和热加工 |
| ES2711176T3 (es) | 2013-11-25 | 2019-04-30 | Gencell Biosystems Ltd | Celdillas con líquido compuesto que se pueden transportar |
| US10722879B2 (en) | 2013-11-25 | 2020-07-28 | Gencell Biosystems Ltd. | Magnetic separation |
| EP3105326A4 (en) | 2014-02-10 | 2018-01-10 | Gencell Biosystems Limited | Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same |
-
2013
- 2013-01-25 ES ES13721381.5T patent/ES2642862T3/es active Active
- 2013-01-25 CN CN201910628323.8A patent/CN110511923A/zh active Pending
- 2013-01-25 EP EP13721381.5A patent/EP2807255B1/en active Active
- 2013-01-25 WO PCT/IB2013/000478 patent/WO2013111016A2/en active Application Filing
- 2013-01-25 CN CN201380016532.8A patent/CN104334725A/zh active Pending
- 2013-01-25 KR KR1020147022894A patent/KR102028225B1/ko active Active
- 2013-01-25 US US14/374,727 patent/US9777269B2/en active Active
- 2013-01-25 AU AU2013213349A patent/AU2013213349B2/en active Active
- 2013-01-25 CA CA2861959A patent/CA2861959A1/en not_active Abandoned
- 2013-01-25 JP JP2014553825A patent/JP6141878B2/ja active Active
- 2013-01-25 BR BR112014018187A patent/BR112014018187A8/pt active Search and Examination
-
2017
- 2017-08-02 US US15/667,568 patent/US10081808B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US9777269B2 (en) | 2017-10-03 |
| EP2807255A2 (en) | 2014-12-03 |
| ES2642862T3 (es) | 2017-11-20 |
| JP6141878B2 (ja) | 2017-06-07 |
| CN110511923A (zh) | 2019-11-29 |
| KR20140120916A (ko) | 2014-10-14 |
| BR112014018187A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2017-06-20 |
| WO2013111016A3 (en) | 2013-10-31 |
| JP2015508154A (ja) | 2015-03-16 |
| KR102028225B1 (ko) | 2019-10-02 |
| US10081808B2 (en) | 2018-09-25 |
| BR112014018187A8 (pt) | 2017-07-11 |
| EP2807255B1 (en) | 2017-08-02 |
| AU2013213349A1 (en) | 2014-08-14 |
| AU2013213349B2 (en) | 2018-02-01 |
| CN104334725A (zh) | 2015-02-04 |
| WO2013111016A2 (en) | 2013-08-01 |
| US20180016573A1 (en) | 2018-01-18 |
| US20140371107A1 (en) | 2014-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10081808B2 (en) | Biomolecule isolation | |
| US9951375B2 (en) | Biomolecule isolation and thermal processing | |
| US12065640B2 (en) | Microfluidic device for extracting, isolating, and analyzing DNA from cells | |
| JP6203876B2 (ja) | 核酸ライブラリーの作製方法 | |
| CN108103057B (zh) | 用于纯化核酸的方法和试剂盒 | |
| Reinholt et al. | Microfluidic isolation of nucleic acids | |
| JP5977921B2 (ja) | 核酸を精製することを目的とした装置、システムおよび方法 | |
| CN104350152A (zh) | 选择性核酸片段回收 | |
| CN103789201A (zh) | 核酸提取用器具、使用其的核酸提取方法、核酸提取用试剂盒以及核酸提取用装置 | |
| JP2017079766A (ja) | 核酸抽出及び分画のためのマイクロ流体デバイス | |
| CA2369083A1 (en) | Biochemical purification devices with immobilized capture probes and their uses | |
| US20190049434A1 (en) | Multi-stage, multiplexed target isolation and processing from heterogeneous populations | |
| WO2018022581A1 (en) | Barcoded systems with multiple information | |
| CN108473983B (zh) | 使用单一洗涤和洗脱缓冲溶液的核酸纯化系统 | |
| US20220372465A1 (en) | Methods and systems for extracting nucleic acids from a biological sample | |
| CN112020564A (zh) | 用于线性样品加工的方法 | |
| Hartanto et al. | Microfluidics-Based DNA Detection | |
| US20100256012A1 (en) | Flexible extraction method for the production of sequence-specific molecule libraries | |
| CN120666033A (en) | Microparticles coupled with PCR primer containing index and application thereof | |
| CN120225283A (zh) | 用于样品处理和光学pcr的方法和系统 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20180105 |
|
| FZDE | Discontinued |
Effective date: 20210831 |
|
| FZDE | Discontinued |
Effective date: 20210831 |