CA2861270A1 - Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes - Google Patents

Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes Download PDF

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Publication number
CA2861270A1
CA2861270A1 CA2861270A CA2861270A CA2861270A1 CA 2861270 A1 CA2861270 A1 CA 2861270A1 CA 2861270 A CA2861270 A CA 2861270A CA 2861270 A CA2861270 A CA 2861270A CA 2861270 A1 CA2861270 A1 CA 2861270A1
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Canada
Prior art keywords
cells
cell
tissue culture
culture fluid
trap
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Granted
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CA2861270A
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English (en)
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CA2861270C (fr
Inventor
Medhi Saghafi
Ricaredo Matanguihan
Chetan Goudar
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Bayer Healthcare LLC
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Bayer Healthcare LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un système de bioréacteur de perfusion comprenant un piège à agrégat cellulaire. Le piège à agrégat cellulaire est configuré pour retirer des agrégats de cellules (par exemple, des amas ou des agrégats supérieurs à environ 10 cellules) du système de bioréacteur à perfusion. Dans certains modes de réalisation, le piège à agrégat cellulaire a une chambre de sédimentation et une chambre d'écoulement latérale permettant à des agrégats de cellules de se séparer, d'être retirés et jetés. Il est prévu des procédés pour faire fonctionner le système de bioréacteur de perfusion ayant un piège à agrégat cellulaire, ainsi que de nombreux autres modes de réalisation.
CA2861270A 2012-01-18 2013-01-15 Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes Active CA2861270C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261587940P 2012-01-18 2012-01-18
US61/587,940 2012-01-18
PCT/US2013/021533 WO2013109520A1 (fr) 2012-01-18 2013-01-15 Systèmes de bioréacteur de perfusion et procédés d'exploitation de ces systèmes

Publications (2)

Publication Number Publication Date
CA2861270A1 true CA2861270A1 (fr) 2013-07-25
CA2861270C CA2861270C (fr) 2021-08-03

Family

ID=47605793

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2861270A Active CA2861270C (fr) 2012-01-18 2013-01-15 Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes

Country Status (6)

Country Link
EP (1) EP2804943A1 (fr)
JP (1) JP6227562B2 (fr)
CN (1) CN104160013B (fr)
CA (1) CA2861270C (fr)
HK (1) HK1199056A1 (fr)
WO (1) WO2013109520A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3047013B1 (fr) * 2013-09-16 2021-08-18 Genzyme Corporation Procédés et systèmes de traitement d'une culture cellulaire
EP3089800A4 (fr) * 2013-12-30 2018-09-12 GE Healthcare Bio-Sciences Corp. Dispositif de culture cellulaire
US20170292104A1 (en) * 2014-09-17 2017-10-12 Massachusetts Institute Of Technology Microfluidic system and method for perfusion bioreactor cell retention
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
WO2017162467A1 (fr) * 2016-03-21 2017-09-28 General Electric Company Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioréacteur à plateforme basculante
CN107541464B (zh) * 2017-10-31 2021-04-30 山东亦度生物技术有限公司 增强型的细胞微载体灌流培养的截留方法
US20210163868A1 (en) * 2018-05-22 2021-06-03 Nantkwest, Inc. Methods and systems for cell bed formation during bioprocessing
JP7087704B2 (ja) * 2018-06-13 2022-06-21 株式会社Ihi 細胞培養システム及び細胞培養方法
US11028359B2 (en) 2018-09-11 2021-06-08 Global Life Sciences Solutions Usa Llc Separation devices, associated methods, and systems
GB201904083D0 (en) * 2019-03-25 2019-05-08 Ge Healthcare Bio Sciences Ab A bioreactor system
EP3835403A1 (fr) * 2019-12-11 2021-06-16 Sartorius Stedim Biotech GmbH Stratégie d'échange automatique de support pour cellules de suspension
JP7406205B2 (ja) * 2020-03-24 2023-12-27 横河電機株式会社 メタン生成装置
WO2024092004A1 (fr) * 2022-10-26 2024-05-02 National Resilience, Inc. Systèmes de bioréacteur et leurs procédés de fonctionnement

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05153973A (ja) * 1991-12-03 1993-06-22 Hitachi Ltd 細胞培養方法及び装置
US6338964B1 (en) 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
CN1112442C (zh) * 2000-06-14 2003-06-25 华东理工大学 细胞灌注培养过程中的细胞截留分离方法及其装置
PT1451290E (pt) * 2001-08-31 2011-03-09 Bayer Schering Pharma Ag Uma unidade e um processo para levar a cabo a fermentação com elevada densidade celular
ES2634323T3 (es) 2004-09-30 2017-09-27 Bayer Healthcare Llc Dispositivos y procedimientos para la fabricación continua integrada de moléculas biológicas
WO2007071072A1 (fr) * 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal Bioréacteur de perfusion à haut rendement
US8512566B2 (en) * 2009-12-11 2013-08-20 General Electric Company Disposable fluid path systems and methods for processing complex biological materials

Also Published As

Publication number Publication date
CA2861270C (fr) 2021-08-03
CN104160013B (zh) 2017-07-28
CN104160013A (zh) 2014-11-19
HK1199056A1 (en) 2015-06-19
JP2015503929A (ja) 2015-02-05
EP2804943A1 (fr) 2014-11-26
WO2013109520A1 (fr) 2013-07-25
JP6227562B2 (ja) 2017-11-08

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