CA2812872C - De-mannosylation of phosphorylated n-glycans - Google Patents

De-mannosylation of phosphorylated n-glycans Download PDF

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Publication number
CA2812872C
CA2812872C CA2812872A CA2812872A CA2812872C CA 2812872 C CA2812872 C CA 2812872C CA 2812872 A CA2812872 A CA 2812872A CA 2812872 A CA2812872 A CA 2812872A CA 2812872 C CA2812872 C CA 2812872C
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Prior art keywords
aspergillus
disease
mannosidase
fungal cell
cell
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CA2812872A
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English (en)
French (fr)
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CA2812872A1 (en
Inventor
Kathleen Camilla Telesphore Alida Maria Piens
Wouter Vervecken
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiteit Gent
Vlaams Instituut voor Biotechnologie VIB
Oxyrane UK Ltd
Original Assignee
Universiteit Gent
Vlaams Instituut voor Biotechnologie VIB
Oxyrane UK Ltd
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Publication of CA2812872A1 publication Critical patent/CA2812872A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01113—Mannosyl-oligosaccharide 1,2-alpha-mannosidase (3.2.1.113), i.e. alpha-1,2-mannosidase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA2812872A 2010-09-29 2011-09-29 De-mannosylation of phosphorylated n-glycans Active CA2812872C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38792410P 2010-09-29 2010-09-29
US61/387,924 2010-09-29
PCT/IB2011/002780 WO2012042387A2 (en) 2010-09-29 2011-09-29 De-mannosylation of phosphorylated n-glycans

Publications (2)

Publication Number Publication Date
CA2812872A1 CA2812872A1 (en) 2012-04-05
CA2812872C true CA2812872C (en) 2021-08-03

Family

ID=45478372

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2812872A Active CA2812872C (en) 2010-09-29 2011-09-29 De-mannosylation of phosphorylated n-glycans

Country Status (9)

Country Link
US (2) US9689015B2 (https=)
EP (1) EP2622089B1 (https=)
JP (1) JP6131190B2 (https=)
KR (1) KR101979220B1 (https=)
CN (1) CN103261432A (https=)
BR (1) BR112013007263A2 (https=)
CA (1) CA2812872C (https=)
SG (1) SG189110A1 (https=)
WO (1) WO2012042387A2 (https=)

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* Cited by examiner, † Cited by third party
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US9347050B2 (en) 2010-09-29 2016-05-24 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated N-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
WO2012042387A2 (en) * 2010-09-29 2012-04-05 Oxyrane Uk Limited De-mannosylation of phosphorylated n-glycans
EP3628326B1 (en) 2012-03-15 2024-02-28 Oxyrane UK Limited Methods and materials for treatment of pompe's disease
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Also Published As

Publication number Publication date
KR101979220B1 (ko) 2019-05-16
JP6131190B2 (ja) 2017-05-17
JP2013538581A (ja) 2013-10-17
US10344310B2 (en) 2019-07-09
KR20140036124A (ko) 2014-03-25
EP2622089A2 (en) 2013-08-07
WO2012042387A3 (en) 2012-09-07
SG189110A1 (en) 2013-05-31
EP2622089C0 (en) 2023-11-01
US20130295603A1 (en) 2013-11-07
WO2012042387A2 (en) 2012-04-05
CA2812872A1 (en) 2012-04-05
CN103261432A (zh) 2013-08-21
US9689015B2 (en) 2017-06-27
EP2622089B1 (en) 2023-11-01
US20170306379A1 (en) 2017-10-26
BR112013007263A2 (pt) 2016-06-14

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