CA2812291C - Methods and reagents for signal amplification - Google Patents
Methods and reagents for signal amplification Download PDFInfo
- Publication number
- CA2812291C CA2812291C CA2812291A CA2812291A CA2812291C CA 2812291 C CA2812291 C CA 2812291C CA 2812291 A CA2812291 A CA 2812291A CA 2812291 A CA2812291 A CA 2812291A CA 2812291 C CA2812291 C CA 2812291C
- Authority
- CA
- Canada
- Prior art keywords
- dextran
- detectable
- dextrans
- avidin
- binding moiety
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 230000003321 amplification Effects 0.000 title description 2
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
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- 239000003431 cross linking reagent Substances 0.000 description 1
- NZNMSOFKMUBTKW-UHFFFAOYSA-N cyclohexanecarboxylic acid Chemical compound OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 1
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- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/02—Dextran; Derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/548—Carbohydrates, e.g. dextran
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Materials Engineering (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38593710P | 2010-09-23 | 2010-09-23 | |
| US61/385,937 | 2010-09-23 | ||
| PCT/US2011/052837 WO2012040514A2 (en) | 2010-09-23 | 2011-09-22 | Methods and reagents for signal amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2812291A1 CA2812291A1 (en) | 2012-03-29 |
| CA2812291C true CA2812291C (en) | 2020-06-16 |
Family
ID=45874393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2812291A Active CA2812291C (en) | 2010-09-23 | 2011-09-22 | Methods and reagents for signal amplification |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9347946B2 (enExample) |
| EP (1) | EP2619588B1 (enExample) |
| JP (3) | JP6234817B2 (enExample) |
| CN (1) | CN103229057B (enExample) |
| CA (1) | CA2812291C (enExample) |
| WO (1) | WO2012040514A2 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9023235B2 (en) | 2012-09-07 | 2015-05-05 | Prestone Products Corporation | Heat transfer fluid additive composition |
| US10955379B2 (en) | 2018-09-27 | 2021-03-23 | Taiwan Semiconductor Manufacturing Co., Ltd. | Differential sensing with BioFET sensors |
| US10984211B1 (en) | 2019-10-18 | 2021-04-20 | Taiwan Semiconductor Manufacturing Co., Ltd. | Semiconductor device with bioFET and biometric sensors |
| CN111830250A (zh) * | 2020-08-04 | 2020-10-27 | 珠海市丽拓生物科技有限公司 | 一种信号放大的酶标二抗的制备方法 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE427505B (sv) * | 1977-03-04 | 1983-04-11 | Pharmacia Diagnostics Ab | Reagens till anvendning vid immunkemiska bestemningsmetoder |
| US4576912A (en) * | 1978-11-30 | 1986-03-18 | Technicon Instruments Corporation | Fluoroimmunoassaying |
| US4615986A (en) * | 1979-03-05 | 1986-10-07 | Syntex (U.S.A.) Inc. | Method for competitive protein binding assays inhibiting non-specific interference |
| US4256834A (en) * | 1979-04-09 | 1981-03-17 | Syva Company | Fluorescent scavenger particle immunoassay |
| US4719182A (en) * | 1985-03-18 | 1988-01-12 | Eastman Kodak Company | Fluorescent labels and labeled species and their use in analytical elements and determinations |
| US6881536B1 (en) * | 1986-04-30 | 2005-04-19 | Bioveris Corporation | Particle based electrochemiluminescent assays |
| DE4140142A1 (de) * | 1991-12-05 | 1993-06-09 | Boehringer Mannheim Gmbh, 6800 Mannheim, De | Multivalentes dextranreagenz zum einsatz in praezipitationstests |
| US5846741A (en) * | 1992-08-21 | 1998-12-08 | Immunomedics, Inc. | Boron neutron capture therapy using pre-targeting methods |
| US5798276A (en) * | 1995-06-07 | 1998-08-25 | Molecular Probes, Inc. | Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
| US5650334A (en) * | 1995-08-31 | 1997-07-22 | First Medical, Inc. | Fluorescent labelling compositions and methods for their use |
| US7659073B2 (en) * | 1997-04-30 | 2010-02-09 | Hybritech Incorporated | Forms of prostate specific antigens and methods for their detection |
| US7288636B2 (en) * | 1997-04-30 | 2007-10-30 | Hybritech Incorporated | Forms of prostate specific antigens and methods for their detection |
| US5994089A (en) * | 1997-05-16 | 1999-11-30 | Coulter International Corp. | Simultaneous analyses of white blood cell subsets using multi-color, multi-intensity fluorescent markers in flow cytometry |
| US5891741A (en) * | 1997-05-16 | 1999-04-06 | Coulter International Corp. | Antibody-aminodextran-phycobiliprotein conjugates |
| WO1999040438A1 (en) | 1998-02-03 | 1999-08-12 | Synbiotics Corporation | Device and method to detect immunoprotective antibody titers |
| AU755968B2 (en) * | 1998-07-30 | 2003-01-02 | Oakville Hong Kong Co., Limited | Method for preparing water-soluble cross-linked conjugates |
| US6284873B1 (en) * | 1999-03-12 | 2001-09-04 | Hybritech Incorporated | Complex of human kallikrein 2 (hK2) and protease inhibitor-6 (PI-6) in prostate tumor tissue and methods of using the complex |
| US8183060B2 (en) * | 2001-09-19 | 2012-05-22 | Daiichi Pure Chemicals Co., Ltd. | Luminescent polymer and use thereof in bioassay |
| CN100425991C (zh) * | 2004-02-25 | 2008-10-15 | 中国科学院动物研究所 | 一种用于检测sars抗体的免疫微球及其制备方法和用途 |
| CA2560016A1 (en) * | 2004-03-17 | 2005-10-13 | Ciphergen Biosystems, Inc. | Photocrosslinked hydrogel blend surface coatings |
| US20060046310A1 (en) | 2004-08-25 | 2006-03-02 | Zong-Li Xia | Amplification method for solid phase immunoassays |
| SE0402476D0 (sv) * | 2004-10-13 | 2004-10-13 | Biacore Ab | Preparation and use of a reactive solid support surface |
| US20070037174A1 (en) * | 2005-08-12 | 2007-02-15 | Rampal Jang B | Chemiluminescent generated fluorescent labeling |
| JP5683069B2 (ja) * | 2005-10-27 | 2015-03-11 | バイオ−ラッド・ハイファ・リミテッド | 結合性層ならびにその調製方法およびその使用 |
| DE112006003813T5 (de) * | 2006-03-20 | 2009-01-22 | Inverness Medical Switzerland Gmbh | Wasserlösliche Konjugate zur elektrochemischen Detektion |
| GB0711560D0 (en) * | 2007-06-14 | 2007-07-25 | Innova Biosciences Ltd | Improvements in or relating to production of conjugates |
| WO2009070742A2 (en) | 2007-11-28 | 2009-06-04 | Great Basin Scientific | Methods and compositions for signal enhancement using multivalent interactions |
| CN101750483B (zh) * | 2008-12-02 | 2013-12-25 | 浙江我武生物科技股份有限公司 | 一种光纤生物传感器探头及其制备方法和应用 |
| US20120100538A1 (en) * | 2009-03-24 | 2012-04-26 | Biocept, Inc. | Devices and methods of cell capture and analysis |
| ES2557914T3 (es) * | 2009-03-24 | 2016-01-29 | Biocept, Inc. | Dispositivos y métodos de captura y análisis de células |
| TWI484186B (zh) * | 2009-05-26 | 2015-05-11 | Ind Tech Res Inst | 使用生物感測器的方法及偵測待測樣本中葡萄糖濃度的套組 |
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| EP2619588A2 (en) | 2013-07-31 |
| WO2012040514A3 (en) | 2012-05-31 |
| JP6234817B2 (ja) | 2017-11-22 |
| WO2012040514A2 (en) | 2012-03-29 |
| JP2015227895A (ja) | 2015-12-17 |
| CN103229057A (zh) | 2013-07-31 |
| EP2619588A4 (en) | 2014-05-07 |
| JP2017205132A (ja) | 2017-11-24 |
| CA2812291A1 (en) | 2012-03-29 |
| US20120164661A1 (en) | 2012-06-28 |
| CN103229057B (zh) | 2016-08-10 |
| US9347946B2 (en) | 2016-05-24 |
| JP2013539860A (ja) | 2013-10-28 |
| EP2619588B1 (en) | 2019-08-07 |
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