CA2748807C - Separation method using single polymer phase systems - Google Patents

Separation method using single polymer phase systems Download PDF

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Publication number
CA2748807C
CA2748807C CA2748807A CA2748807A CA2748807C CA 2748807 C CA2748807 C CA 2748807C CA 2748807 A CA2748807 A CA 2748807A CA 2748807 A CA2748807 A CA 2748807A CA 2748807 C CA2748807 C CA 2748807C
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CA
Canada
Prior art keywords
phase
polymer
mab
eopo
target
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Active
Application number
CA2748807A
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English (en)
French (fr)
Other versions
CA2748807A1 (en
Inventor
James Van Alstine
Jamil Shanagar
Rolf Hjorth
Martin Hall
Camilla Estmer Nilsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
GE Healthcare Bioprocess R&D AB
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Publication of CA2748807A1 publication Critical patent/CA2748807A1/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/04—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from milk
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2748807A 2009-01-08 2010-01-07 Separation method using single polymer phase systems Active CA2748807C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900014 2009-01-08
SE0900014-2 2009-01-08
PCT/SE2010/050008 WO2010080062A1 (en) 2009-01-08 2010-01-07 Separation method using single polymer phase systems

Publications (2)

Publication Number Publication Date
CA2748807A1 CA2748807A1 (en) 2010-07-15
CA2748807C true CA2748807C (en) 2019-01-29

Family

ID=42316654

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2748807A Active CA2748807C (en) 2009-01-08 2010-01-07 Separation method using single polymer phase systems

Country Status (7)

Country Link
US (1) US9115181B2 (https=)
EP (1) EP2373676B1 (https=)
JP (1) JP5785098B2 (https=)
CN (1) CN102272144B (https=)
AU (1) AU2010203836B2 (https=)
CA (1) CA2748807C (https=)
WO (1) WO2010080062A1 (https=)

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BRPI0920572A8 (pt) 2008-10-20 2015-10-27 Abbott Lab Inativação viral durante a purificação dos anticorpos
NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
BR112013007237A2 (pt) 2010-11-05 2016-06-14 Hoffmann La Roche ''método para produzir um anticorpo anti-ig-1r''
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1207960A1 (en) 2013-03-12 2016-02-19 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9828599B2 (en) 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US20150093839A1 (en) 2013-10-02 2015-04-02 General Electric Company Methods and systems for detection of target and applications thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101745455B1 (ko) * 2015-03-24 2017-06-09 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 다단계 정제방법
KR101761680B1 (ko) * 2015-03-31 2017-08-23 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 분리방법
WO2018165309A1 (en) 2017-03-08 2018-09-13 The Regents Of The University Of Michigan Analyte detection
EP4234710A3 (en) 2017-03-28 2023-11-01 Phase Scientific International, Ltd. Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
SG11201909324WA (en) * 2017-06-01 2019-11-28 Yin To Chiu Phase separation behavior modifying agents for aqueous two-phase separation within porous material
US10450345B2 (en) 2017-06-09 2019-10-22 General Electric Company Method of isolation of polypeptide-aptamer-polymer conjugates
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
ES2964100T3 (es) 2017-09-18 2024-04-04 Phase Scient International Ltd Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos
EP3740588A4 (en) 2018-01-19 2021-10-20 Phase Scientific International, Ltd. METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM
WO2019143895A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Spontaneous nucleic acid purification and concentration in a single step
WO2019143943A2 (en) 2018-01-19 2019-07-25 Yin To Chiu Composition and method for concentration and enrichment of nucleic acids
WO2019191497A1 (en) * 2018-03-30 2019-10-03 Blaze Bioscience, Inc. Systems and methods for simultaneous near-infrared light and visible light imaging
US12172108B2 (en) 2018-08-16 2024-12-24 Emd Millipore Corporation Closed bioprocessing device
EP4121530A1 (en) 2020-03-20 2023-01-25 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction
CN112321704A (zh) * 2020-11-20 2021-02-05 华东理工大学 利用液态热响应型聚合物eo20po80分离纯化单克隆抗体
CN116840009A (zh) * 2023-07-01 2023-10-03 相达生物科技(深圳)有限公司 一种新型冠状病毒样本预处理方法
WO2025193698A1 (en) * 2024-03-11 2025-09-18 Michigan Technological University Purification of viral particles with two stage aqueous two-phase extraction

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US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
CA2356704A1 (en) * 1998-12-30 2000-07-13 Folke Tjerneld Separation method utilizing liquid-liquid partition
US6544788B2 (en) * 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
WO2007073311A1 (en) * 2005-12-22 2007-06-28 Ge Healthcare Bio-Sciences Ab Preparation of biomolecules
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems
JP2010530462A (ja) * 2007-06-19 2010-09-09 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー ポリマー２相システムおよびその使用

Also Published As

Publication number Publication date
EP2373676A1 (en) 2011-10-12
JP2012514637A (ja) 2012-06-28
US9115181B2 (en) 2015-08-25
EP2373676B1 (en) 2017-04-19
AU2010203836B2 (en) 2015-05-28
WO2010080062A1 (en) 2010-07-15
CN102272144A (zh) 2011-12-07
JP5785098B2 (ja) 2015-09-24
EP2373676A4 (en) 2013-02-27
CA2748807A1 (en) 2010-07-15
AU2010203836A1 (en) 2011-07-07
US20120010390A1 (en) 2012-01-12
CN102272144B (zh) 2014-09-17

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