AU2010203836B2 - Separation method using single polymer phase systems - Google Patents

Separation method using single polymer phase systems Download PDF

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Publication number
AU2010203836B2
AU2010203836B2 AU2010203836A AU2010203836A AU2010203836B2 AU 2010203836 B2 AU2010203836 B2 AU 2010203836B2 AU 2010203836 A AU2010203836 A AU 2010203836A AU 2010203836 A AU2010203836 A AU 2010203836A AU 2010203836 B2 AU2010203836 B2 AU 2010203836B2
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AU
Australia
Prior art keywords
phase
polymer
eopo
target
mab
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AU2010203836A
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AU2010203836A1 (en
Inventor
Camilla Estmer Nilsson
Martin Hall
Rolf Hjorth
Jamil Shanagar
James Van Alstine
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Cytiva Bioprocess R&D AB
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Cytiva Bioprocess R&D AB
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Assigned to CYTIVA BIOPROCESS R&D AB reassignment CYTIVA BIOPROCESS R&D AB Request to Amend Deed and Register Assignors: GE HEALTHCARE BIOPROCESS R&D AB
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/04—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from milk
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2010203836A 2009-01-08 2010-01-07 Separation method using single polymer phase systems Active AU2010203836B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900014 2009-01-08
SE0900014-2 2009-01-08
PCT/SE2010/050008 WO2010080062A1 (en) 2009-01-08 2010-01-07 Separation method using single polymer phase systems

Publications (2)

Publication Number Publication Date
AU2010203836A1 AU2010203836A1 (en) 2011-07-07
AU2010203836B2 true AU2010203836B2 (en) 2015-05-28

Family

ID=42316654

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010203836A Active AU2010203836B2 (en) 2009-01-08 2010-01-07 Separation method using single polymer phase systems

Country Status (7)

Country Link
US (1) US9115181B2 (https=)
EP (1) EP2373676B1 (https=)
JP (1) JP5785098B2 (https=)
CN (1) CN102272144B (https=)
AU (1) AU2010203836B2 (https=)
CA (1) CA2748807C (https=)
WO (1) WO2010080062A1 (https=)

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BRPI0920572A8 (pt) 2008-10-20 2015-10-27 Abbott Lab Inativação viral durante a purificação dos anticorpos
NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
BR112013007237A2 (pt) 2010-11-05 2016-06-14 Hoffmann La Roche ''método para produzir um anticorpo anti-ig-1r''
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1207960A1 (en) 2013-03-12 2016-02-19 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9828599B2 (en) 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US20150093839A1 (en) 2013-10-02 2015-04-02 General Electric Company Methods and systems for detection of target and applications thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101745455B1 (ko) * 2015-03-24 2017-06-09 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 다단계 정제방법
KR101761680B1 (ko) * 2015-03-31 2017-08-23 포항공과대학교 산학협력단 수용액 이상계를 이용한 세포 밖 소포체의 분리방법
WO2018165309A1 (en) 2017-03-08 2018-09-13 The Regents Of The University Of Michigan Analyte detection
EP4234710A3 (en) 2017-03-28 2023-11-01 Phase Scientific International, Ltd. Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
SG11201909324WA (en) * 2017-06-01 2019-11-28 Yin To Chiu Phase separation behavior modifying agents for aqueous two-phase separation within porous material
US10450345B2 (en) 2017-06-09 2019-10-22 General Electric Company Method of isolation of polypeptide-aptamer-polymer conjugates
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
ES2964100T3 (es) 2017-09-18 2024-04-04 Phase Scient International Ltd Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos
EP3740588A4 (en) 2018-01-19 2021-10-20 Phase Scientific International, Ltd. METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM
WO2019143895A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Spontaneous nucleic acid purification and concentration in a single step
WO2019143943A2 (en) 2018-01-19 2019-07-25 Yin To Chiu Composition and method for concentration and enrichment of nucleic acids
WO2019191497A1 (en) * 2018-03-30 2019-10-03 Blaze Bioscience, Inc. Systems and methods for simultaneous near-infrared light and visible light imaging
US12172108B2 (en) 2018-08-16 2024-12-24 Emd Millipore Corporation Closed bioprocessing device
EP4121530A1 (en) 2020-03-20 2023-01-25 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction
CN112321704A (zh) * 2020-11-20 2021-02-05 华东理工大学 利用液态热响应型聚合物eo20po80分离纯化单克隆抗体
CN116840009A (zh) * 2023-07-01 2023-10-03 相达生物科技(深圳)有限公司 一种新型冠状病毒样本预处理方法
WO2025193698A1 (en) * 2024-03-11 2025-09-18 Michigan Technological University Purification of viral particles with two stage aqueous two-phase extraction

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WO2008156410A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems

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Patent Citations (3)

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US6454950B1 (en) * 1998-12-30 2002-09-24 Amersham Pharmacia Biotech Ab Separation method utilizing liquid-liquid partition
WO2008156410A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems

Non-Patent Citations (6)

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Title
ALRED P. A., et al, "Application of temperature-induced phase partitioning at ambient temperature for enzyme purification", Journal of Chromatography A, 1994, vol 659, pages 289-298 *
AZEVEDO A. M., et al. Journal of Chromatography A, 2008, vol 1213, pages 154-161 *
HARRIS P. A., et al, "Enzyme purification using temperature-induced phase formation", Bioseparation, 1991, vol 2, pages 237-246 *
JOHANSSON H-O., et al, "Thermoseparating water/polymer system: a novel one-polymer aqueous two-phase system for protein purification", Biotechnology and bioengineering, 1999, vol 66, pages 247-257 # *
LU JIN ET AL.: "Preparation and Application of Novel EOPO-IDA-Metal Polymer as Recyclable Metal Affinity Ligand in Aqueous Two-Phase Systems", IND.ENG.CHEM.RES., vol. 45, 2006, pages 1774 - 1779, XP008152931 *
PERSSON J., et al. Journal of Chromatography B, 1998, vol 711, pages 97-109 *

Also Published As

Publication number Publication date
EP2373676A1 (en) 2011-10-12
JP2012514637A (ja) 2012-06-28
US9115181B2 (en) 2015-08-25
EP2373676B1 (en) 2017-04-19
WO2010080062A1 (en) 2010-07-15
CN102272144A (zh) 2011-12-07
JP5785098B2 (ja) 2015-09-24
EP2373676A4 (en) 2013-02-27
CA2748807A1 (en) 2010-07-15
CA2748807C (en) 2019-01-29
AU2010203836A1 (en) 2011-07-07
US20120010390A1 (en) 2012-01-12
CN102272144B (zh) 2014-09-17

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Owner name: GE HEALTHCARE BIOPROCESS R&D AB

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Owner name: CYTIVA BIOPROCESS R&D AB

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