CA2748117A1 - Method for detection of xmrv - Google Patents
Method for detection of xmrv Download PDFInfo
- Publication number
- CA2748117A1 CA2748117A1 CA2748117A CA2748117A CA2748117A1 CA 2748117 A1 CA2748117 A1 CA 2748117A1 CA 2748117 A CA2748117 A CA 2748117A CA 2748117 A CA2748117 A CA 2748117A CA 2748117 A1 CA2748117 A1 CA 2748117A1
- Authority
- CA
- Canada
- Prior art keywords
- xmrv
- nucleotide sequence
- complementary
- forward primer
- reverse primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 82
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 238000003752 polymerase chain reaction Methods 0.000 claims abstract description 90
- 206010060862 Prostate cancer Diseases 0.000 claims abstract description 72
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims abstract description 66
- 210000002307 prostate Anatomy 0.000 claims abstract description 37
- 238000003753 real-time PCR Methods 0.000 claims abstract description 33
- 210000002700 urine Anatomy 0.000 claims abstract description 25
- 230000028327 secretion Effects 0.000 claims abstract description 20
- 239000002773 nucleotide Substances 0.000 claims description 252
- 125000003729 nucleotide group Chemical group 0.000 claims description 252
- 239000000523 sample Substances 0.000 claims description 183
- 230000000295 complement effect Effects 0.000 claims description 152
- 230000002441 reversible effect Effects 0.000 claims description 128
- 210000001519 tissue Anatomy 0.000 claims description 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 9
- 210000004908 prostatic fluid Anatomy 0.000 claims description 7
- 241001663880 Gammaretrovirus Species 0.000 claims description 5
- 238000003757 reverse transcription PCR Methods 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 238000001502 gel electrophoresis Methods 0.000 claims description 4
- 238000012544 monitoring process Methods 0.000 claims description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 claims description 3
- 206010005003 Bladder cancer Diseases 0.000 claims description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 2
- 201000005112 urinary bladder cancer Diseases 0.000 claims description 2
- 238000010367 cloning Methods 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 abstract description 24
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract description 15
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract description 15
- 102000039446 nucleic acids Human genes 0.000 abstract description 15
- 108020004707 nucleic acids Proteins 0.000 abstract description 15
- 238000004458 analytical method Methods 0.000 abstract description 5
- 241000713893 Xenotropic murine leukemia virus Species 0.000 abstract description 4
- 239000013615 primer Substances 0.000 description 247
- 108020004414 DNA Proteins 0.000 description 64
- 230000003321 amplification Effects 0.000 description 31
- 238000003199 nucleic acid amplification method Methods 0.000 description 31
- 238000006243 chemical reaction Methods 0.000 description 27
- 108090000623 proteins and genes Proteins 0.000 description 16
- 102000053602 DNA Human genes 0.000 description 14
- 206010028980 Neoplasm Diseases 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 102100027962 2-5A-dependent ribonuclease Human genes 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 238000003556 assay Methods 0.000 description 11
- 230000027455 binding Effects 0.000 description 10
- 108091034117 Oligonucleotide Proteins 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 101001080057 Homo sapiens 2-5A-dependent ribonuclease Proteins 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 238000011471 prostatectomy Methods 0.000 description 7
- 108010006785 Taq Polymerase Proteins 0.000 description 6
- 238000001574 biopsy Methods 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000013068 control sample Substances 0.000 description 6
- 208000020735 familial prostate carcinoma Diseases 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108010000834 2-5A-dependent ribonuclease Proteins 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 239000011543 agarose gel Substances 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- 239000002987 primer (paints) Substances 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 108091093088 Amplicon Proteins 0.000 description 4
- 230000006820 DNA synthesis Effects 0.000 description 4
- 239000013614 RNA sample Substances 0.000 description 4
- 238000011529 RT qPCR Methods 0.000 description 4
- 208000005074 Retroviridae Infections Diseases 0.000 description 4
- 238000000137 annealing Methods 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- VLEIUWBSEKKKFX-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O VLEIUWBSEKKKFX-UHFFFAOYSA-N 0.000 description 3
- 108020004635 Complementary DNA Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000002123 RNA extraction Methods 0.000 description 3
- 238000010802 RNA extraction kit Methods 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 239000007984 Tris EDTA buffer Substances 0.000 description 3
- 108010020713 Tth polymerase Proteins 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000010804 cDNA synthesis Methods 0.000 description 3
- 239000013522 chelant Substances 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 210000001165 lymph node Anatomy 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 238000007857 nested PCR Methods 0.000 description 3
- 239000013610 patient sample Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 208000023958 prostate neoplasm Diseases 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000005382 thermal cycling Methods 0.000 description 3
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 2
- 241000283073 Equus caballus Species 0.000 description 2
- 101710154606 Hemagglutinin Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 2
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 238000002944 PCR assay Methods 0.000 description 2
- 102000007066 Prostate-Specific Antigen Human genes 0.000 description 2
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 2
- 101710176177 Protein A56 Proteins 0.000 description 2
- -1 RNA Chemical compound 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 101150077651 VP35 gene Proteins 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 231100000504 carcinogenesis Toxicity 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 238000013480 data collection Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000185 hemagglutinin Substances 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 229910001425 magnesium ion Inorganic materials 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 201000007094 prostatitis Diseases 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 239000003161 ribonuclease inhibitor Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000001625 seminal vesicle Anatomy 0.000 description 2
- 108010068698 spleen exonuclease Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 201000010653 vesiculitis Diseases 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- UDGUGZTYGWUUSG-UHFFFAOYSA-N 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-n-methylanilino]butanoic acid Chemical compound COC=1C=C(N=NC=2C=CC(=CC=2)N(C)CCCC(O)=O)C(OC)=CC=1N=NC1=CC=C([N+]([O-])=O)C=C1 UDGUGZTYGWUUSG-UHFFFAOYSA-N 0.000 description 1
- NJYVEMPWNAYQQN-UHFFFAOYSA-N 5-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C21OC(=O)C1=CC(C(=O)O)=CC=C21 NJYVEMPWNAYQQN-UHFFFAOYSA-N 0.000 description 1
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000714177 Murine leukemia virus Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- 208000037062 Polyps Diseases 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000589500 Thermus aquaticus Species 0.000 description 1
- 108010001244 Tli polymerase Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- MKUXAQIIEYXACX-UHFFFAOYSA-N aciclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCO)C=N2 MKUXAQIIEYXACX-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 210000004100 adrenal gland Anatomy 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000133 brain stem Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000005773 cancer-related death Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000001638 cerebellum Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 108700004025 env Genes Proteins 0.000 description 1
- 101150030339 env gene Proteins 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000007387 excisional biopsy Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 238000007849 hot-start PCR Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000007386 incisional biopsy Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 238000001948 isotopic labelling Methods 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 235000019689 luncheon sausage Nutrition 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001915 proofreading effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011472 radical prostatectomy Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20355608P | 2008-12-23 | 2008-12-23 | |
| US61/203,556 | 2008-12-23 | ||
| PCT/US2009/069244 WO2010075414A2 (en) | 2008-12-23 | 2009-12-22 | Method for detection of xmrv |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2748117A1 true CA2748117A1 (en) | 2010-07-01 |
Family
ID=42096470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2748117A Abandoned CA2748117A1 (en) | 2008-12-23 | 2009-12-22 | Method for detection of xmrv |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20100184015A1 (OSRAM) |
| EP (1) | EP2382326A2 (OSRAM) |
| JP (1) | JP2012513216A (OSRAM) |
| CA (1) | CA2748117A1 (OSRAM) |
| WO (1) | WO2010075414A2 (OSRAM) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2007012361A (es) * | 2005-04-07 | 2008-02-11 | Cleveland Clinic Foundation | Gamaretrovirus asociado con cancer. |
| EP2448960A1 (en) | 2009-06-30 | 2012-05-09 | Abbott Laboratories | Markers of xmrv infection and uses thereof |
| US9410189B2 (en) * | 2010-04-30 | 2016-08-09 | Co-Diagnostics, Inc. | Methods of preventing non-specific reactions of nucleotide sequences |
| CA2803011C (en) * | 2010-07-16 | 2019-12-03 | Tocagen Inc. | Retrovirus detection |
| WO2012024513A2 (en) * | 2010-08-18 | 2012-02-23 | Abbott Laboratories | Molecular detection of xmrv infection |
| WO2012024518A1 (en) * | 2010-08-18 | 2012-02-23 | Abbott Laboratories | Molecular detection of xmrv infection |
| WO2012030856A2 (en) * | 2010-08-30 | 2012-03-08 | Gen-Probe Incorporated | Compositions, methods and reaction mixtures for the detection of xenotropic murine leukemia virus-related virus |
| GB201019043D0 (en) | 2010-11-10 | 2010-12-22 | Protea Biopharma N V | Use of 2',5'-oligoadenylate derivative compounds |
| US20130065222A1 (en) * | 2011-08-30 | 2013-03-14 | Gen-Probe Incorporated | Compositions, methods and reaction mixtures for the detection of murine leukemia virus-related virus |
| CN102337359B (zh) * | 2011-11-02 | 2014-02-19 | 舒泰神（北京）生物制药股份有限公司 | 用于检测小鼠白血病病毒的引物、探针及其方法 |
| MX2019001186A (es) * | 2016-07-29 | 2019-08-29 | Juno Therapeutics Inc | Metodos para evaluar la presencia o la ausencia de un virus capaz de replicacion. |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10306234B4 (de) | 2003-02-04 | 2009-09-17 | Daimler Ag | Verfahren zur Luftversorgung einer Brennstoffzelle und Vorrichtung zur Durchführung des Verfahrens |
| MX2007012361A (es) * | 2005-04-07 | 2008-02-11 | Cleveland Clinic Foundation | Gamaretrovirus asociado con cancer. |
| US20110151431A1 (en) * | 2009-06-18 | 2011-06-23 | Whittemore Peterson Institute For Neuro-Immune Disease | Detection of xenotropic murine leukemia virus |
| EP2448960A1 (en) * | 2009-06-30 | 2012-05-09 | Abbott Laboratories | Markers of xmrv infection and uses thereof |
-
2009
- 2009-12-22 JP JP2011543647A patent/JP2012513216A/ja active Pending
- 2009-12-22 CA CA2748117A patent/CA2748117A1/en not_active Abandoned
- 2009-12-22 US US12/645,181 patent/US20100184015A1/en not_active Abandoned
- 2009-12-22 EP EP09804357A patent/EP2382326A2/en not_active Withdrawn
- 2009-12-22 WO PCT/US2009/069244 patent/WO2010075414A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010075414A2 (en) | 2010-07-01 |
| JP2012513216A (ja) | 2012-06-14 |
| US20100184015A1 (en) | 2010-07-22 |
| WO2010075414A3 (en) | 2010-10-07 |
| EP2382326A2 (en) | 2011-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20100184015A1 (en) | Method for detection of xmrv | |
| JP7766398B2 (ja) | マイクロサテライト配列中の突然変異を検出する方法 | |
| JP6983201B2 (ja) | 核酸プローブ | |
| US9249459B2 (en) | Single cell nucleic acid analysis | |
| CN114277135A (zh) | 胃癌淋巴结转移相关的甲基化生物标记物及其组合和检测试剂盒 | |
| CN113832258A (zh) | 使用衰减型探针的核糖核酸扩增和检测 | |
| JP2018528780A (ja) | 短いホモポリマー反復の改良された検出 | |
| AU2005250479B2 (en) | Diagnosing or predicting the course of breast cancer | |
| RU2558236C2 (ru) | Система анализа для обнаружения близкородственных серотипов вируса папилломы человека (впч) | |
| JP5258760B2 (ja) | メチル化核酸又は非メチル化核酸を増幅する方法 | |
| KR101761862B1 (ko) | 표적 핵산에 대한 검출 정확도를 높이기 위한 프로브 세트 및 상기 프로브 세트를 이용하여 표적 핵산을 검출하는 방법 | |
| KR20210113083A (ko) | SARS-CoV-2 진단용 조성물, 키트 및 이를 이용한 SARS-CoV-2의 진단방법 | |
| CN103562411B (zh) | 细环病毒诊断学 | |
| CN118451202A (zh) | 一种肿瘤检测方法及应用 | |
| EP1530644B1 (en) | Probes for detecting tumor cells | |
| CN118414438A (zh) | 使用内部定量标准物定量核酸序列的组合物、试剂盒和方法 | |
| US20150307923A1 (en) | Megasphaera cerevisiae system process control (spc) primers, probes, and methods | |
| CN102171369A (zh) | 存活蛋白mRNA的测定方法 | |
| US20240352544A1 (en) | Compositions, kits, and methods for detection of nucleic acid sequence loads | |
| TW202338101A (zh) | 用於檢測膀胱癌之存在之引子及探針 | |
| CN111411156A (zh) | 用于癌症早期检测的试剂盒及检测方法 | |
| JP2008517626A (ja) | 乳房細胞を検出するための核酸プライマー及びプローブ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20141223 |