CA2733990C - Biomarker detection process and assay of neurological condition - Google Patents
Biomarker detection process and assay of neurological condition Download PDFInfo
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| EP3779444A1 (en) | 2008-01-18 | 2021-02-17 | President and Fellows of Harvard College | Methods of detecting signatures of disease or conditions in bodily fluids |
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| AU2011354597B2 (en) | 2010-01-26 | 2016-09-22 | Bioregency Inc. | Compositions and methods relating to argininosuccinate synthetase |
| CN102918397A (zh) * | 2010-04-01 | 2013-02-06 | 班扬生物标记公司 | 用于神经毒性检测的标志物和测定方法 |
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| AU2013273984A1 (en) | 2012-06-15 | 2015-01-22 | Harry Stylli | Methods of detecting diseases or conditions using circulating diseased cells |
| BR112014031365A2 (pt) | 2012-06-15 | 2017-06-27 | Stylli Harry | métodos de detectar doenças ou condições |
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| CN105229467A (zh) * | 2013-03-15 | 2016-01-06 | 普林斯顿大学理事会 | 快速且灵敏的分析物测量测定法 |
| US20150141528A1 (en) * | 2013-05-31 | 2015-05-21 | Banyan Biomarkers, Inc. | Neural specific s100b for biomarker assays and devices for detection of a neurological condition |
| JP6611710B2 (ja) | 2013-07-17 | 2019-11-27 | ザ・ジョンズ・ホプキンス・ユニバーシティ | 脳損傷の検出および転帰についてのマルチプロテインバイオマーカー測定法 |
| GB2548942A (en) * | 2014-04-08 | 2017-10-04 | Univ Florida | Protein biomarkers for acute, subacute and chronic traumatic injuries of the central nervous system |
| EP3191846A4 (en) | 2014-09-11 | 2018-06-13 | Harry Stylli | Methods of detecting prostate cancer |
| AU2015330355B2 (en) * | 2014-10-06 | 2021-09-23 | Fundacio Hospital Universitari Vall D'hebron - Institut De Recerca | Markers and their use in brain injury |
| WO2017100632A1 (en) * | 2015-12-11 | 2017-06-15 | Arizona Board Of Regents On Behalf Of Arizona State University | Human alzheimer's disease and traumatic brain injury associated tau variants as biomarkers and methods of use thereof |
| JP2019535015A (ja) * | 2016-10-03 | 2019-12-05 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | 患者サンプルにおけるｇｆａｐ状況を評価する改善された方法 |
| CN110366558A (zh) | 2016-10-28 | 2019-10-22 | 班扬生物标记公司 | 针对泛素c末端水解酶l1(uch-l1)和胶质纤维酸性蛋白(gfap)的抗体及相关方法 |
| JP2020507622A (ja) * | 2017-02-07 | 2020-03-12 | バイオリージェンシー インコーポレイテッド | 神経学的状態検出のためのＳ１００β及びアイソフォーム |
| US10345302B2 (en) * | 2017-02-19 | 2019-07-09 | Sheng-He Huang | Circulating astrocytes and MFSD2A as biomarkers |
| JP7346300B2 (ja) * | 2017-03-23 | 2023-09-19 | アボット・ラボラトリーズ | 早期バイオマーカーであるユビキチンカルボキシ末端ヒドロラーゼｌ１を使用する、ヒト対象における外傷性脳損傷の程度の診断及び決定の一助となるための方法 |
| EP3602069A1 (en) * | 2017-03-23 | 2020-02-05 | Abbott Laboratories | Methods for aiding in the diagnosis and determination of the extent of traumatic brain injury in a human subject using the early biomarker ubiquitin carboxy-terminal hydrolase l1 |
| WO2018191531A1 (en) | 2017-04-15 | 2018-10-18 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury in a human subject using early biomarkers |
| WO2018200823A1 (en) | 2017-04-28 | 2018-11-01 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury using early biomarkers on at least two samples from the same human subject |
| AU2018272054B2 (en) | 2017-05-25 | 2024-10-31 | Abbott Laboratories | Methods for aiding in the determination of whether to perform imaging on a human subject who has sustained or may have sustained an injury to the head using early biomarkers |
| CA3059607A1 (en) * | 2017-05-30 | 2018-12-06 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a mild traumatic brain injury in a human subject using cardiac troponin i |
| EP3649474A1 (en) | 2017-07-03 | 2020-05-13 | Abbott Laboratories | Improved methods for measuring ubiquitin carboxy-terminal hydrolase l1 levels in blood |
| US11685951B2 (en) | 2017-07-18 | 2023-06-27 | The Research Foundation For The State University Of New York | Biomarkers for intracranial aneurysm |
| US11016105B2 (en) | 2017-12-09 | 2021-05-25 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a traumatic brain injury in a human subject using a combination of GFAP and UCH-L1 |
| CA3067057A1 (en) * | 2017-12-09 | 2019-06-13 | Abbott Laboratories | Methods for aiding in the diagnosis and evaluation of a subject who has sustained an orthopedic injury and that has or may have sustained an injury to the head, such as mild traumatic brain injury (tbi), using glial fibrillary acidic protein (gfap) and/or ubiquitin carboxy-terminal hydrolase l1 (uch-l1) |
| JP7437303B2 (ja) | 2017-12-29 | 2024-02-22 | アボット・ラボラトリーズ | 外傷性脳損傷を診断及び査定するための、新規のバイオマーカー及び方法 |
| WO2019199865A1 (en) * | 2018-04-10 | 2019-10-17 | Quanterix Corporation | Quantification of biomarkers present in dried physiological samples |
| KR102179032B1 (ko) * | 2019-09-20 | 2020-11-18 | 한림대학교 산학협력단 | 급성허혈성뇌손상 및 급성외상성뇌손상을 구별하기 위한 바이오마커 및 이의 이용 |
| MX2022009517A (es) * | 2020-02-10 | 2022-09-02 | Somalogic Operating Co Inc | Biomarcadores de esteatohepatitis no alcoholica (nash) y usos de los mismos. |
| JP2023551542A (ja) | 2020-11-30 | 2023-12-08 | エニグマ バイオインテリジェンス，インコーポレイテッド | アルツハイマー病の非侵襲的評価 |
| JP2023173693A (ja) * | 2022-05-26 | 2023-12-07 | キヤノンメディカルシステムズ株式会社 | 診断支援装置、診断支援方法及びプログラム |
| CN116879564A (zh) * | 2023-09-06 | 2023-10-13 | 暨南大学附属第一医院（广州华侨医院） | 基于蛋白质组学及磷酸化蛋白质修饰组学的脊髓损伤生物标志物及其应用 |
| WO2025188813A1 (en) * | 2024-03-04 | 2025-09-12 | Conan Medtech Corp | Devices, systems, compositions, and methods for diagnosis of traumatic brain injury |
Family Cites Families (120)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4376110A (en) | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4517288A (en) | 1981-01-23 | 1985-05-14 | American Hospital Supply Corp. | Solid phase system for ligand assay |
| US4702909A (en) | 1982-05-05 | 1987-10-27 | Louisiana State University A & M | Non-A, non-B hepatitis antigen, antigen compositions, vaccine and diagnostic reagent |
| CA1291031C (en) | 1985-12-23 | 1991-10-22 | Nikolaas C.J. De Jaeger | Method for the detection of specific binding agents and their correspondingbindable substances |
| US5231000A (en) | 1987-10-08 | 1993-07-27 | The Mclean Hospital | Antibodies to A4 amyloid peptide |
| US5118606A (en) | 1988-09-02 | 1992-06-02 | The Regents Of The University Of California | Methods for detecting cellular pathology by assaying spectrin and spectrin breakdown products |
| US5234814A (en) | 1989-06-01 | 1993-08-10 | Du Pont Merck Pharmaceutical Company | Diagnostic assay for alzheimer's disease |
| GB2236186B (en) | 1989-08-22 | 1994-01-05 | Finnigan Mat Gmbh | Process and device for laser desorption of analyte molecular ions, especially of biomolecules |
| US5045694A (en) | 1989-09-27 | 1991-09-03 | The Rockefeller University | Instrument and method for the laser desorption of ions in mass spectrometry |
| JPH05506777A (ja) | 1990-03-05 | 1993-10-07 | セファロン、インコーポレーテッド | キモトリプシン様プロテアーゼ及びそれらの阻害剤 |
| US5586226A (en) | 1990-05-16 | 1996-12-17 | Canon Kabushiki Kaisha | Control method and device for a unicolor printer |
| US5707796A (en) * | 1990-06-11 | 1998-01-13 | Nexstar Pharmaceuticals, Inc. | Method for selecting nucleic acids on the basis of structure |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US5496938A (en) | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
| US5660985A (en) | 1990-06-11 | 1997-08-26 | Nexstar Pharmaceuticals, Inc. | High affinity nucleic acid ligands containing modified nucleotides |
| US6011020A (en) | 1990-06-11 | 2000-01-04 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand complexes |
| ES2110444T3 (es) * | 1990-06-11 | 1998-02-16 | Nexstar Pharmaceuticals Inc | Ligandos de acidos nucleicos. |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5637459A (en) | 1990-06-11 | 1997-06-10 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chimeric selex |
| US5683867A (en) | 1990-06-11 | 1997-11-04 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: blended SELEX |
| US5252463A (en) | 1990-06-22 | 1993-10-12 | The Du Pont Merck Pharmaceutical Company | Clipsin, a chymotrypsin-like protease and method of using same |
| EP0600951A4 (en) | 1991-08-01 | 1996-10-30 | Paul H Voorheis | Diagnostic method for alzheimer's disease. |
| US5792664A (en) | 1992-05-29 | 1998-08-11 | The Rockefeller University | Methods for producing and analyzing biopolymer ladders |
| CA2136717A1 (en) | 1992-05-29 | 1993-12-09 | Brian T. Chait | Method and product for the sequence determination of peptides using a mass spectrometer |
| EP1347493A3 (en) | 1993-05-28 | 2005-11-23 | Baylor College Of Medicine | Method and apparatus for desorption and ionization of analytes |
| DE4337654C2 (de) | 1993-11-04 | 1996-08-08 | Biotest Pharma Gmbh | Verwendung boviner Kolostralmilch von nicht mit Viren hyperimmunisierten Tieren als Leberschutzpräparat |
| US5536639A (en) | 1994-03-25 | 1996-07-16 | Cephalon, Inc. | Methods for detecting calpain activation by detection of calpain activated spectrin breakdown products |
| DE69533135T2 (de) * | 1994-07-15 | 2005-06-16 | Cephalon, Inc. | In baculovirus exprimiertes aktives calpain |
| US5614649A (en) * | 1994-11-14 | 1997-03-25 | Cephalon, Inc. | Multicatalytic protease inhibitors |
| US5777194A (en) * | 1995-04-26 | 1998-07-07 | Cephalon, Inc. | Gene-targeted mice with humanized Aβ sequence and Swedish FAD mutation |
| EP0880700B1 (en) | 1996-02-02 | 2006-03-29 | Biotrin Intellectual Properties Limited | Method of determining the hepatic status of a liver transplant recipient. |
| US6048703A (en) * | 1996-11-15 | 2000-04-11 | Cephalon, Inc. | Methods for detecting cell apoptosis |
| NZ516848A (en) | 1997-06-20 | 2004-03-26 | Ciphergen Biosystems Inc | Retentate chromatography apparatus with applications in biology and medicine |
| US20010012854A1 (en) | 1997-12-16 | 2001-08-09 | Robert Siman | Multicatalytic protease inhibitors for use as anti-tumor agents |
| US6537749B2 (en) | 1998-04-03 | 2003-03-25 | Phylos, Inc. | Addressable protein arrays |
| US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| DE69920487T2 (de) | 1998-09-08 | 2006-03-09 | Innogenetics N.V. | Tau als marker für frühe schäden des zentralen nervensystems |
| WO2000056934A1 (en) | 1999-03-24 | 2000-09-28 | Packard Bioscience Company | Continuous porous matrix arrays |
| US6218005B1 (en) * | 1999-04-01 | 2001-04-17 | 3M Innovative Properties Company | Tapes for heat sealing substrates |
| US20020002270A1 (en) | 1999-06-16 | 2002-01-03 | Raymond P. Zinkowski | Purified antigen for alzheimer's disease, and methods of obtaining and using same |
| US6589746B1 (en) | 1999-10-21 | 2003-07-08 | University Of Cincinnati | Method of detecting axonally-derived protein tau in patients with traumatic CNS injury |
| WO2001075165A2 (en) * | 2000-03-30 | 2001-10-11 | Elan Pharmaceuticals, Inc. | Screening markers and methods for neurodegenerative disorders |
| US20050063942A1 (en) | 2001-02-02 | 2005-03-24 | Clark Mike A. | Methods for predicting sensitivity of tumors to arginine deprivation |
| US6949377B2 (en) | 2001-03-05 | 2005-09-27 | Ho Winston Z | Chemiluminescence-based microfluidic biochip |
| US7713705B2 (en) | 2002-12-24 | 2010-05-11 | Biosite, Inc. | Markers for differential diagnosis and methods of use thereof |
| US20040253637A1 (en) | 2001-04-13 | 2004-12-16 | Biosite Incorporated | Markers for differential diagnosis and methods of use thereof |
| US20040203083A1 (en) | 2001-04-13 | 2004-10-14 | Biosite, Inc. | Use of thrombus precursor protein and monocyte chemoattractant protein as diagnostic and prognostic indicators in vascular diseases |
| US20030199000A1 (en) | 2001-08-20 | 2003-10-23 | Valkirs Gunars E. | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
| US20020172676A1 (en) | 2001-05-16 | 2002-11-21 | George Jackowski | Method of treatment of alzheimer's disease and device therefor |
| US7144708B2 (en) | 2001-06-25 | 2006-12-05 | The Cleveland Clinic Foundation | Markers of blood barrier disruption and methods of using same |
| JP2003070498A (ja) | 2001-07-19 | 2003-03-11 | Pharma Design Inc | ユビキチンカルボキシルターミナルヒドロラーゼｌ１遺伝子異常の検出方法 |
| DE60233949D1 (de) * | 2001-08-20 | 2009-11-19 | Biosite Inc | Diagnostische marker für schlaganfall und hirntrauma sowie verfahren zur verwendung davon |
| US20030040660A1 (en) | 2001-08-27 | 2003-02-27 | George Jackowski | Method for diagnosing and distinguishing traumatic brain injury and diagnostic devices for use therein |
| AU2002329570A1 (en) | 2001-10-12 | 2003-01-30 | Pfizer Products Inc. | Method of monitoring neuroprotective treatment |
| US7371582B2 (en) | 2002-01-23 | 2008-05-13 | Boditechmed Inc. | Lateral flow quantitative assay method and strip and laser-induced fluorescence detection device therefor |
| CA2471661A1 (en) | 2002-01-31 | 2003-08-07 | Gene Logic, Inc. | Molecular hepatotoxicology modeling |
| EP1495419A2 (en) | 2002-04-01 | 2005-01-12 | Phase-1 Molecular Toxicology Inc. | Liver necrosis predictive genes |
| WO2004078204A1 (en) | 2002-06-12 | 2004-09-16 | The Cleveland Clinic Foundation | Markers of blood barrier permeability and methods of using same |
| AU2003270509B2 (en) * | 2002-09-11 | 2009-03-26 | University Of Florida | Analyzing nerve cell damage |
| EP1587955A4 (en) | 2002-12-24 | 2007-03-14 | Biosite Inc | DIFFERENTIAL DIAGNOSTIC MARKERS AND METHODS OF USE |
| EP1635763B1 (en) | 2003-06-09 | 2012-08-08 | Alnylam Pharmaceuticals Inc. | Method of treating neurodegenerative disease |
| DK2341350T3 (en) | 2003-09-20 | 2018-02-05 | Electrophoretics Ltd | DIAGNOSTIC PROCEDURE FOR BRAKE INJURY-RELATED DISORDERS BASED ON DJ-1 DETECTION |
| GB0322063D0 (en) * | 2003-09-20 | 2003-10-22 | Univ Geneve | Diagnostic method for brain damage-related disorders |
| EP1519194A1 (en) | 2003-09-24 | 2005-03-30 | Roche Diagnostics GmbH | Use of gfap for identification of intracerebral hemorrhage |
| US20050130226A1 (en) | 2003-09-26 | 2005-06-16 | The University Of Cincinnati | Fully integrated protein lab-on-a-chip with smart microfluidics for spot array generation |
| JP4796967B2 (ja) | 2003-11-07 | 2011-10-19 | ヴァーミリオン インコーポレイテッド | アルツハイマー病のためのバイオマーカー |
| US20060094064A1 (en) | 2003-11-19 | 2006-05-04 | Sandip Ray | Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids |
| US20050112585A1 (en) | 2003-11-21 | 2005-05-26 | Dominic Zichi | Method for adjusting the quantification range of individual analytes in a multiplexed assay |
| TWI281473B (en) | 2003-12-19 | 2007-05-21 | Ind Tech Res Inst | Biomarkers for liver diseases and method for using same |
| CA2564609C (en) | 2003-12-31 | 2014-02-11 | President And Fellows Of Harvard College | Assay device and method |
| US20140303041A1 (en) | 2004-04-15 | 2014-10-09 | University Of Florida Research Foundation Inc. | In vitro diagnostic devices for nervous system injury and other neural disorders |
| JP4659025B2 (ja) * | 2004-04-15 | 2011-03-30 | ユニバーシティ オブ フロリダ リサーチ ファンデーション インコーポレーティッド | 神経系傷害および他の神経障害についての生物マーカーとしての神経タンパク質 |
| US8492107B2 (en) | 2004-04-15 | 2013-07-23 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for nervous system injury and other neural disorders |
| US7456027B2 (en) * | 2004-04-15 | 2008-11-25 | University Of Florida Research Foundation, Inc. | Proteolytic biomarkers for traumatic injury to the nervous system |
| US20060292558A1 (en) | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for protein assay diagnostics |
| EP1796838B1 (de) | 2004-10-15 | 2014-10-08 | Boehringer Ingelheim Vetmedica GmbH | Verfahren zur durchführung einer elektrochemischen messung an einer flüssigen messprobe in einer über leitungen zugänglichen messkammer |
| US8048638B2 (en) | 2005-04-01 | 2011-11-01 | University Of Florida Research Foundation, Inc. | Biomarkers of liver injury |
| EP1874958B1 (en) | 2005-04-01 | 2019-03-27 | University of Florida Research Foundation, Incorporated | Biomarkers of liver injury |
| GB2428240A (en) | 2005-07-14 | 2007-01-24 | Univ Gen Ve | Diagnostic method for brain damage-related disorders |
| WO2007033385A2 (en) | 2005-09-13 | 2007-03-22 | Fluidigm Corporation | Microfluidic assay devices and methods |
| EP1949103A2 (en) | 2005-10-20 | 2008-07-30 | Biosite Incorporated | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
| US9023278B2 (en) | 2006-01-12 | 2015-05-05 | Micah James Atkin | Instrumentation systems and methods |
| US8563291B2 (en) | 2006-02-15 | 2013-10-22 | Mie University | Method of constructing recombinant proteoliposome for diagnostic use |
| US20070255113A1 (en) | 2006-05-01 | 2007-11-01 | Grimes F R | Methods and apparatus for identifying disease status using biomarkers |
| WO2007136617A2 (en) | 2006-05-18 | 2007-11-29 | Walter Reed Army Institute Of Research (Wrair) | Endothelial-monocyte activating polypeptide ii, a biomarker for use in diagnosis of brain injury |
| WO2007140188A2 (en) | 2006-05-26 | 2007-12-06 | Biosite Incorporated | Use of natriuretic peptides as diagnostic and prognostic indicators in vascular diseases |
| WO2008008819A2 (en) | 2006-07-11 | 2008-01-17 | University Of Florida Research Foundation, Inc. | Diagnosis and treatment of neurological inflammation |
| CA2668640A1 (en) | 2006-11-01 | 2008-05-29 | George Mason Intellectual Properties, Inc. | Biomarkers for neurological conditions |
| WO2008095136A2 (en) | 2007-01-31 | 2008-08-07 | Henkin Robert I | Methods for detection of biological substances |
| US20120196307A1 (en) | 2007-02-06 | 2012-08-02 | Banyan Biomarkers, Inc. | Synaptotagmin and collapsin response mediator protein as biomarkers for traumatic brain injury |
| WO2008097618A1 (en) | 2007-02-06 | 2008-08-14 | University Of Florida Research Foundation, Inc. | Synaptotagmin and collapsin response mediator protein as biomarkers for traumatic brain injury |
| CA2715248A1 (en) | 2008-02-04 | 2009-08-13 | Banyan Biomarkers, Inc. | Process to diagnose or treat brain injury |
| US20110097392A1 (en) | 2008-04-17 | 2011-04-28 | Banyan Biomarkers, Inc | Antibody bound synthetic vesicle containing molecules for deliver to central and peripheral nervous system cells |
| EP2143735A1 (en) | 2008-07-10 | 2010-01-13 | Institut Pasteur | Variable domains of camelid heavy-chain antibodies directed against glial fibrillary acidic proteins |
| US20140342381A1 (en) | 2008-08-11 | 2014-11-20 | Banyan Biomarkers, Inc. | Devices and methods for biomarker detection process and assay of neurological condition |
| NO2324360T3 (en:Method) * | 2008-08-11 | 2018-06-30 | ||
| WO2010033912A2 (en) | 2008-09-19 | 2010-03-25 | Henry Ford Health System | Methods, systems, and compositions for calpain inhibition |
| US20120094295A1 (en) | 2008-11-21 | 2012-04-19 | The Johns Hopkins University | Neurodegenerative disease diagnostic compositions and methods of use |
| EP2443461A4 (en) | 2009-06-19 | 2012-12-26 | Banyan Biomarkers Inc | BIOMARKER ASSAY FOR NERVES |
| US20170315136A9 (en) | 2009-06-19 | 2017-11-02 | Banyan Biomarkers, Inc. | Biomarker assay of neurological condition |
| WO2011011334A2 (en) | 2009-07-18 | 2011-01-27 | Banyan Biomarkers, Inc. | SYNERGISTIC BIOMARKER ASSAY OF NEUROLGICAL CONDITION USING S-100β |
| HUE040281T2 (hu) | 2009-09-14 | 2019-03-28 | Banyan Biomarkers Inc | Autoantitest markerek traumás agysérülés diagnózisára |
| AU2011354597B2 (en) | 2010-01-26 | 2016-09-22 | Bioregency Inc. | Compositions and methods relating to argininosuccinate synthetase |
| CN102918397A (zh) | 2010-04-01 | 2013-02-06 | 班扬生物标记公司 | 用于神经毒性检测的标志物和测定方法 |
| WO2011160096A2 (en) | 2010-06-17 | 2011-12-22 | Banyan Biomarkers, Inc. | Glial fibrillary acidic protein, autoantigens and autoantibodies thereto as biomarkers of neural injury or neurological disorder or condition |
| WO2012051519A2 (en) | 2010-10-14 | 2012-04-19 | The Johns Hopkins University | Biomarkers of brain injury |
| CA2846625A1 (en) | 2011-09-14 | 2013-03-21 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. | Processes and kits to detect and monitor for diagnostic biomarkers for post traumatic stress disorder (ptsd) and to differentiate between suicidal and non-suicidal form of the disorder |
| US20140018299A1 (en) | 2012-07-10 | 2014-01-16 | Banyan Biomarkers, Inc. | Method and device to detect, monitor and promote neural regeneration and improvement of cognitive function in a subject suffering from neural injury |
| US20140024053A1 (en) | 2012-07-20 | 2014-01-23 | Banyan Biomarkers, Inc. | Methods, kits and devices for detecting bii-spectrin, and breakdown products thereof, as biomarkers for the diagnosis of neural injury |
| US20140275294A1 (en) | 2013-03-15 | 2014-09-18 | Banyan Biomarkers, Inc. | Devices and methods for biomarker detection process and assay of liver injury |
| US20150141528A1 (en) | 2013-05-31 | 2015-05-21 | Banyan Biomarkers, Inc. | Neural specific s100b for biomarker assays and devices for detection of a neurological condition |
| WO2015157300A1 (en) | 2014-04-07 | 2015-10-15 | Iron Horse Diagnostics, Inc | Traumatic brain injury and neurodegenerative biomarkers, methods, and systems |
| GB2548942A (en) | 2014-04-08 | 2017-10-04 | Univ Florida | Protein biomarkers for acute, subacute and chronic traumatic injuries of the central nervous system |
| CN110366558A (zh) | 2016-10-28 | 2019-10-22 | 班扬生物标记公司 | 针对泛素c末端水解酶l1(uch-l1)和胶质纤维酸性蛋白(gfap)的抗体及相关方法 |
| WO2018191531A1 (en) * | 2017-04-15 | 2018-10-18 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury in a human subject using early biomarkers |
| WO2018200823A1 (en) * | 2017-04-28 | 2018-11-01 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury using early biomarkers on at least two samples from the same human subject |
| CA3059607A1 (en) * | 2017-05-30 | 2018-12-06 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a mild traumatic brain injury in a human subject using cardiac troponin i |
| CA3067057A1 (en) * | 2017-12-09 | 2019-06-13 | Abbott Laboratories | Methods for aiding in the diagnosis and evaluation of a subject who has sustained an orthopedic injury and that has or may have sustained an injury to the head, such as mild traumatic brain injury (tbi), using glial fibrillary acidic protein (gfap) and/or ubiquitin carboxy-terminal hydrolase l1 (uch-l1) |
| US11016105B2 (en) * | 2017-12-09 | 2021-05-25 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a traumatic brain injury in a human subject using a combination of GFAP and UCH-L1 |
-
2009
- 2009-08-11 NO NO09807153A patent/NO2324360T3/no unknown
- 2009-08-11 EP EP09807153.3A patent/EP2324360B1/en not_active Revoked
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- 2009-08-11 JP JP2011523089A patent/JP5781436B2/ja active Active
- 2009-08-11 WO PCT/US2009/053376 patent/WO2010019553A2/en not_active Ceased
- 2009-08-11 US US13/058,748 patent/US20110143375A1/en not_active Abandoned
- 2009-08-11 CA CA2733990A patent/CA2733990C/en active Active
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2014
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2017
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2020
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| EP3336551B1 (en) | 2023-05-31 |
| EP3336551A1 (en) | 2018-06-20 |
| JP5781436B2 (ja) | 2015-09-24 |
| JP2014199262A (ja) | 2014-10-23 |
| ES2665245T3 (es) | 2018-04-25 |
| EP2324360A2 (en) | 2011-05-25 |
| AU2009282117B2 (en) | 2016-05-12 |
| EP2324360A4 (en) | 2012-08-29 |
| EP4235181A3 (en) | 2024-02-28 |
| WO2010019553A2 (en) | 2010-02-18 |
| WO2010019553A3 (en) | 2010-07-08 |
| AU2009282117A1 (en) | 2010-02-18 |
| JP5976732B2 (ja) | 2016-08-24 |
| NO2324360T3 (en:Method) | 2018-06-30 |
| JP2012500388A (ja) | 2012-01-05 |
| US11994522B2 (en) | 2024-05-28 |
| CA2733990A1 (en) | 2010-02-18 |
| US20210011028A1 (en) | 2021-01-14 |
| EP2324360B1 (en) | 2018-01-31 |
| US20110143375A1 (en) | 2011-06-16 |
| EP4235181A2 (en) | 2023-08-30 |
| DK2324360T3 (en) | 2018-05-14 |
| US20180031577A1 (en) | 2018-02-01 |
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