CA2729559C - Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same - Google Patents
Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same Download PDFInfo
- Publication number
- CA2729559C CA2729559C CA2729559A CA2729559A CA2729559C CA 2729559 C CA2729559 C CA 2729559C CA 2729559 A CA2729559 A CA 2729559A CA 2729559 A CA2729559 A CA 2729559A CA 2729559 C CA2729559 C CA 2729559C
- Authority
- CA
- Canada
- Prior art keywords
- multicellular
- bodies
- cells
- multicellular bodies
- filler
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 134
- 239000000945 filler Substances 0.000 claims abstract description 288
- 239000000560 biocompatible material Substances 0.000 claims abstract description 20
- 230000005012 migration Effects 0.000 claims abstract description 18
- 238000013508 migration Methods 0.000 claims abstract description 18
- 210000004027 cell Anatomy 0.000 claims description 581
- 210000002889 endothelial cell Anatomy 0.000 claims description 93
- 210000000329 smooth muscle myocyte Anatomy 0.000 claims description 85
- 210000002950 fibroblast Anatomy 0.000 claims description 80
- 239000003104 tissue culture media Substances 0.000 claims description 20
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 210000002460 smooth muscle Anatomy 0.000 claims description 5
- 230000004927 fusion Effects 0.000 abstract description 19
- 238000007639 printing Methods 0.000 abstract description 11
- 210000001519 tissue Anatomy 0.000 description 69
- 229920000936 Agarose Polymers 0.000 description 55
- 238000007493 shaping process Methods 0.000 description 55
- 239000000463 material Substances 0.000 description 45
- 230000035800 maturation Effects 0.000 description 43
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 33
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 33
- 210000002744 extracellular matrix Anatomy 0.000 description 32
- 239000000243 solution Substances 0.000 description 20
- 108010010803 Gelatin Proteins 0.000 description 19
- 239000008273 gelatin Substances 0.000 description 19
- 229920000159 gelatin Polymers 0.000 description 19
- 235000019322 gelatine Nutrition 0.000 description 19
- 235000011852 gelatine desserts Nutrition 0.000 description 19
- 239000007788 liquid Substances 0.000 description 18
- 230000002792 vascular Effects 0.000 description 18
- 239000002609 medium Substances 0.000 description 17
- 239000000203 mixture Substances 0.000 description 16
- 210000000056 organ Anatomy 0.000 description 16
- 239000000499 gel Substances 0.000 description 15
- 230000008569 process Effects 0.000 description 15
- 108010049003 Fibrinogen Proteins 0.000 description 14
- 102000008946 Fibrinogen Human genes 0.000 description 14
- 210000004204 blood vessel Anatomy 0.000 description 14
- 238000000151 deposition Methods 0.000 description 14
- 229940012952 fibrinogen Drugs 0.000 description 14
- 239000000758 substrate Substances 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 12
- 239000006285 cell suspension Substances 0.000 description 11
- 238000004113 cell culture Methods 0.000 description 10
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 9
- 239000002953 phosphate buffered saline Substances 0.000 description 9
- 239000004809 Teflon Substances 0.000 description 8
- 229920006362 Teflon® Polymers 0.000 description 8
- 230000001413 cellular effect Effects 0.000 description 8
- 230000008021 deposition Effects 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 102000009123 Fibrin Human genes 0.000 description 6
- 108010073385 Fibrin Proteins 0.000 description 6
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 6
- 239000000853 adhesive Substances 0.000 description 6
- 230000001070 adhesive effect Effects 0.000 description 6
- 229950003499 fibrin Drugs 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 5
- 229940088710 antibiotic agent Drugs 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000012091 fetal bovine serum Substances 0.000 description 5
- 210000005119 human aortic smooth muscle cell Anatomy 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 102000003952 Caspase 3 Human genes 0.000 description 4
- 108090000397 Caspase 3 Proteins 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 4
- 229930182566 Gentamicin Natural products 0.000 description 4
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 229960002518 gentamicin Drugs 0.000 description 4
- 239000000017 hydrogel Substances 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 230000008611 intercellular interaction Effects 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 229960005322 streptomycin Drugs 0.000 description 4
- 230000032258 transport Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 3
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 102000016942 Elastin Human genes 0.000 description 3
- 108010014258 Elastin Proteins 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- 239000012981 Hank's balanced salt solution Substances 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 108090000190 Thrombin Proteins 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 238000012407 engineering method Methods 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 238000001879 gelation Methods 0.000 description 3
- 229920002674 hyaluronan Polymers 0.000 description 3
- 229960003160 hyaluronic acid Drugs 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 230000017074 necrotic cell death Effects 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 230000010412 perfusion Effects 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229960004072 thrombin Drugs 0.000 description 3
- 239000011800 void material Substances 0.000 description 3
- FLBAYUMRQUHISI-UHFFFAOYSA-N 1,8-naphthyridine Chemical compound N1=CC=CC2=CC=CN=C21 FLBAYUMRQUHISI-UHFFFAOYSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 108010081589 Becaplermin Proteins 0.000 description 2
- 102000000905 Cadherin Human genes 0.000 description 2
- 108050007957 Cadherin Proteins 0.000 description 2
- 241000699802 Cricetulus griseus Species 0.000 description 2
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 2
- 108010067306 Fibronectins Proteins 0.000 description 2
- 102000016359 Fibronectins Human genes 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- 108010085895 Laminin Proteins 0.000 description 2
- 102000007547 Laminin Human genes 0.000 description 2
- 108010067787 Proteoglycans Proteins 0.000 description 2
- 102000016611 Proteoglycans Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229960003767 alanine Drugs 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 210000004413 cardiac myocyte Anatomy 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 2
- 210000001612 chondrocyte Anatomy 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 238000009295 crossflow filtration Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000007499 fusion processing Methods 0.000 description 2
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 210000003494 hepatocyte Anatomy 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 230000028709 inflammatory response Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000000663 muscle cell Anatomy 0.000 description 2
- 210000003098 myoblast Anatomy 0.000 description 2
- 230000002107 myocardial effect Effects 0.000 description 2
- 239000002121 nanofiber Substances 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 229960002429 proline Drugs 0.000 description 2
- 238000010188 recombinant method Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 210000001626 skin fibroblast Anatomy 0.000 description 2
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- -1 10% porcine serum Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 108010067225 Cell Adhesion Molecules Proteins 0.000 description 1
- 102000016289 Cell Adhesion Molecules Human genes 0.000 description 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108050000637 N-cadherin Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 102000003800 Selectins Human genes 0.000 description 1
- 108090000184 Selectins Proteins 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 210000003892 absorptive cell Anatomy 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 210000003433 aortic smooth muscle cell Anatomy 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000025611 cell-substrate adhesion Effects 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 210000002236 cellular spheroid Anatomy 0.000 description 1
- 238000009750 centrifugal casting Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960005188 collagen Drugs 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 210000001608 connective tissue cell Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000009749 continuous casting Methods 0.000 description 1
- 210000000555 contractile cell Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000005137 deposition process Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 210000004177 elastic tissue Anatomy 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 210000003976 gap junction Anatomy 0.000 description 1
- 229940014259 gelatin Drugs 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000005003 heart tissue Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 108010082117 matrigel Proteins 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001002 morphogenetic effect Effects 0.000 description 1
- 210000003061 neural cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 210000004738 parenchymal cell Anatomy 0.000 description 1
- 229940056360 penicillin g Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000000250 revascularization Effects 0.000 description 1
- 210000004116 schwann cell Anatomy 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000008477 smooth muscle tissue growth Effects 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002948 striated muscle cell Anatomy 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000009772 tissue formation Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 210000003606 umbilical vein Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/225—Fibrin; Fibrinogen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3625—Vascular tissue, e.g. heart valves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/069—Vascular Endothelial cells
- C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1338—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from smooth muscle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/56—Fibrin; Thrombin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/76—Agarose, agar-agar
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Vascular Medicine (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Physiology (AREA)
- Clinical Laboratory Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- Urology & Nephrology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2949615A CA2949615A1 (en) | 2008-06-24 | 2009-06-24 | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13297708P | 2008-06-24 | 2008-06-24 | |
| US61/132,977 | 2008-06-24 | ||
| PCT/US2009/048530 WO2010008905A2 (en) | 2008-06-24 | 2009-06-24 | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2949615A Division CA2949615A1 (en) | 2008-06-24 | 2009-06-24 | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2729559A1 CA2729559A1 (en) | 2010-01-21 |
| CA2729559C true CA2729559C (en) | 2017-01-03 |
Family
ID=41550964
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2729559A Expired - Fee Related CA2729559C (en) | 2008-06-24 | 2009-06-24 | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
| CA2949615A Abandoned CA2949615A1 (en) | 2008-06-24 | 2009-06-24 | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2949615A Abandoned CA2949615A1 (en) | 2008-06-24 | 2009-06-24 | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
Country Status (8)
| Country | Link |
|---|---|
| US (6) | US8143055B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2310847B1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP5624981B2 (cg-RX-API-DMAC7.html) |
| KR (4) | KR101711354B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN102124331B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2009271223B2 (cg-RX-API-DMAC7.html) |
| CA (2) | CA2729559C (cg-RX-API-DMAC7.html) |
| WO (1) | WO2010008905A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6262229B1 (en) * | 1996-12-04 | 2001-07-17 | Biomeasure Incorporated | Somatostatin antagonists |
| WO2005081970A2 (en) | 2004-02-24 | 2005-09-09 | The Curators Of The University Of Missouri | Self-assembling cell aggregates and methods of making engineered tissue using the same |
| EP2310847B1 (en) | 2008-06-24 | 2016-05-04 | The Curators Of The University Of Missouri | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
| US8747880B2 (en) | 2010-02-02 | 2014-06-10 | The Curators Of The University Of Missouri | Engineered biological nerve graft, fabrication and application thereof |
| US9267099B2 (en) | 2010-03-15 | 2016-02-23 | Indiana University Research And Technology Corporation | Engineered lumenized vascular networks and support matrix |
| GB2478801B (en) * | 2010-03-16 | 2012-05-30 | Organovo Inc | Multilayered vascular tubes |
| WO2011140441A2 (en) | 2010-05-06 | 2011-11-10 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| US20120089238A1 (en) * | 2010-10-06 | 2012-04-12 | Hyun-Wook Kang | Integrated organ and tissue printing methods, system and apparatus |
| BR112013009744A2 (pt) | 2010-10-21 | 2016-07-19 | Organovo Inc | dispositivo, sistemas e métodos para a fabricação de tecido |
| ES2685638T3 (es) | 2011-07-26 | 2018-10-10 | The Curators Of The University Of Missouri | Carne comestible producida artificialmente |
| HK1200483A1 (en) * | 2011-09-12 | 2015-08-07 | 奥加诺沃公司 | Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same |
| AU2012308724A1 (en) * | 2011-09-12 | 2014-04-24 | Organovo, Inc. | Platform for engineered implantable tissues and organs and methods of making the same |
| CN103857787B (zh) * | 2011-09-27 | 2019-05-17 | 公立大学法人横滨市立大学 | 组织和器官的制作方法 |
| KR101374478B1 (ko) * | 2012-01-18 | 2014-03-13 | 주승연 | 세포 블록 제조용 조성물, 키트, 및 이들을 이용한 세포 블록 제조 방법 |
| US9499779B2 (en) | 2012-04-20 | 2016-11-22 | Organovo, Inc. | Devices, systems, and methods for the fabrication of tissue utilizing UV cross-linking |
| US20140099709A1 (en) * | 2012-06-19 | 2014-04-10 | Organovo, Inc. | Engineered three-dimensional connective tissue constructs and methods of making the same |
| WO2014039938A1 (en) * | 2012-09-07 | 2014-03-13 | Modern Meadow, Inc. | Spherical multicellular aggregates with endogenous extracellular matrix |
| US9442105B2 (en) | 2013-03-15 | 2016-09-13 | Organovo, Inc. | Engineered liver tissues, arrays thereof, and methods of making the same |
| US20160022870A1 (en) * | 2013-03-15 | 2016-01-28 | Cyfuse Biomedical K.K. | Cardiac or vascular tissue spheroid |
| JP6332782B2 (ja) * | 2013-06-07 | 2018-05-30 | 国立大学法人 鹿児島大学 | 細胞凝集塊作製法 |
| CN103350572B (zh) * | 2013-07-18 | 2016-05-25 | 符晓友 | 3d堆叠打印方法及3d堆叠打印机 |
| AU2014296246B2 (en) | 2013-07-31 | 2021-01-21 | Organovo, Inc. | Automated devices, systems, and methods for the fabrication of tissue |
| WO2015038988A1 (en) | 2013-09-13 | 2015-03-19 | Modern Meadow, Inc. | Edible and animal-product-free microcarriers for engineered meat |
| US20160297104A1 (en) | 2013-11-19 | 2016-10-13 | Guill Tool & Engineering | Coextruded, multilayer and multicomponent 3d printing inputs field |
| CA2938156C (en) | 2014-02-05 | 2022-05-10 | Modern Meadow, Inc. | Dried food products formed from cultured muscle cells |
| AU2014389440A1 (en) | 2014-04-04 | 2016-10-20 | Oregon Health & Science University | Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model |
| ES2860423T3 (es) | 2014-05-28 | 2021-10-05 | Childrens Hospital Med Ct | Métodos y sistemas para convertir células precursoras en tejidos gástricos mediante diferenciación dirigida |
| EP2949350B1 (en) | 2014-05-29 | 2022-05-11 | Sabanci Üniversitesi | Artificial hollow biological tissue network and method for preparation thereof |
| US10669525B2 (en) | 2014-09-29 | 2020-06-02 | Public University Corporation Yokohama City University | Method for producing three-dimensional cell aggregates |
| EP3204488B1 (en) | 2014-10-06 | 2019-07-17 | Organovo, Inc. | Engineered renal tissues, arrays thereof, and methods of making the same |
| WO2016061464A1 (en) | 2014-10-17 | 2016-04-21 | Children's Hospital Center, D/B/A Cincinnati Children's Hospital Medical Center | In vivo model of human small intetine using pluripotent stem cells and methods of making and using same |
| MA40598A (fr) | 2014-11-05 | 2017-09-13 | Oreal | Tissus cutanés tridimensionnels manipulés, ensembles correspondants et leurs procédés de production |
| CN104490489B (zh) * | 2014-12-02 | 2017-02-01 | 淮安皓运生物科技有限公司 | 基于3d生物打印技术制备组织工程血管的方法 |
| SG11201704439QA (en) * | 2014-12-30 | 2017-06-29 | Agency Science Tech & Res | Technique for formation and assembly of 3d cellular structures |
| CN105983134A (zh) * | 2015-03-05 | 2016-10-05 | 刘畅 | 一种人工血管及其制备方法 |
| CN104880408B (zh) * | 2015-04-21 | 2018-05-01 | 上海交通大学医学院附属第九人民医院 | 无创性动态监测材料降解速率与组织再生匹配程度的方法 |
| CN104840272B (zh) * | 2015-05-11 | 2016-09-07 | 浙江大学 | 一种具有内置营养通道的三维生物结构的打印方法 |
| CN104906636B (zh) * | 2015-05-19 | 2018-01-12 | 河海大学常州校区 | 一种三维管状多细胞结构的制备方法 |
| JP6629332B2 (ja) * | 2015-08-24 | 2020-01-15 | Ｎｅｃソリューションイノベータ株式会社 | 細胞インク、細胞インクの製造容器、細胞インクの製造キット、細胞インクの製造方法、インクカートリッジ、立体造形物の製造方法、および立体造形物 |
| EP3862086A1 (en) | 2015-08-31 | 2021-08-11 | Cellink AB | Clean chamber technology for 3d printers and bioprinters |
| ES2842501T5 (es) | 2015-09-21 | 2023-04-13 | Modern Meadow Inc | Materiales compuestos de tejido reforzados con fibras |
| EP3356517A4 (en) * | 2015-10-02 | 2019-04-03 | Wake Forest University Health Sciences | SPONTANEOUS HERB ORGANOID CONSTRUCTS AND INTEGRATED BODY ON CHIP DEVICE WITH IT |
| CN106606804B (zh) * | 2015-10-22 | 2020-05-12 | 四川蓝光英诺生物科技股份有限公司 | 一种制备复合结构的方法 |
| AU2016352873B2 (en) | 2015-11-09 | 2023-01-05 | Organovo, Inc. | Improved methods for tissue fabrication |
| US11542374B2 (en) | 2016-02-15 | 2023-01-03 | Modern Meadow, Inc. | Composite biofabricated material |
| EP4218911A1 (en) | 2016-04-14 | 2023-08-02 | The Trustees of the University of Pennsylvania | Implantable living electrodes and methods for use thereof |
| JP6963882B2 (ja) | 2016-05-05 | 2021-11-10 | チルドレンズ ホスピタル メディカル センター | 胃底部組織のインビトロでの製造のための方法及び当該方法と関連した組成物 |
| WO2017204235A1 (ja) * | 2016-05-24 | 2017-11-30 | 日本電信電話株式会社 | 微小粒子を内包する三次元薄膜構造体およびその製造方法 |
| WO2017214736A1 (en) * | 2016-06-16 | 2017-12-21 | Aspect Biosystems Ltd. | Bioprinted meniscus implant and methods of using same |
| WO2018013727A1 (en) | 2016-07-12 | 2018-01-18 | Deka Products Limited Partnership | System and method for applying force to a device |
| US11254901B2 (en) | 2016-07-12 | 2022-02-22 | Deka Products Limited Partnership | System and method for printing tissue |
| EP3534907A4 (en) | 2016-11-04 | 2020-06-24 | Children's Hospital Medical Center | COMPOSITIONS AND METHODS FOR TREATING HEPATIC DISEASE |
| US11299705B2 (en) | 2016-11-07 | 2022-04-12 | Deka Products Limited Partnership | System and method for creating tissue |
| KR20190077407A (ko) | 2016-11-10 | 2019-07-03 | 오가노보, 인크. | 바이오프린팅된 모발 모낭 및 그의 용도 |
| JP7300986B2 (ja) | 2016-11-10 | 2023-06-30 | オルガノボ インコーポレイテッド | 人工腸組織およびその使用 |
| JP6889363B2 (ja) * | 2016-12-05 | 2021-06-18 | 澁谷工業株式会社 | 細胞の集合構造体の作製方法および作製装置 |
| EP3548507A4 (en) | 2016-12-05 | 2020-07-15 | Children's Hospital Medical Center | COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF |
| KR101849737B1 (ko) * | 2016-12-27 | 2018-04-18 | 한국기계연구원 | 인공 장 및 그 제조 방법 |
| WO2018187380A1 (en) | 2017-04-03 | 2018-10-11 | Greene Nguyen Deborah Lynn | Use of engineered liver tissue constructs for modeling liver disorders |
| EP3609997A4 (en) | 2017-04-14 | 2021-03-03 | Children's Hospital Medical Center | COMPOSITIONS OF STEM CELLS FROM MULTIPLE DONOR CELLS AND THEIR PREPARATION PROCEDURES |
| US12398358B2 (en) * | 2017-05-30 | 2025-08-26 | The Trustees Of Princeton University | Three-dimensional (3D) tissue scaffold with cell alignment |
| US20180356398A1 (en) | 2017-06-09 | 2018-12-13 | Fujifilm Corporation | Living tissue model device, vascular wall model, vascular wall model device and method of evaluating test substance |
| US12297416B2 (en) | 2017-07-12 | 2025-05-13 | Deka Products Limited Partneship | System and method for transferring tissue |
| US10570362B2 (en) | 2017-07-12 | 2020-02-25 | Deka Products Limited Partnership | System and method for transferring tissue |
| CA3077442A1 (en) | 2017-10-10 | 2019-04-18 | Children's Hospital Medical Center | Esophageal tissue and/or organoid compositions and methods of making same |
| CN111386335A (zh) * | 2017-10-30 | 2020-07-07 | 公立大学法人横滨市立大学 | 结构体与细胞团块连接的构建物 |
| AU2018253595A1 (en) | 2017-11-13 | 2019-05-30 | Modern Meadow, Inc. | Biofabricated leather articles having zonal properties |
| US12029834B2 (en) | 2017-12-20 | 2024-07-09 | Aspect Biosystems Ltd. | Bioprinted meniscus implant and methods of using same |
| WO2019126626A1 (en) | 2017-12-21 | 2019-06-27 | Children's Hospital Medical Center | Digitalized human organoids and methods of using same |
| SG11202100533VA (en) | 2018-07-26 | 2021-02-25 | Childrens Hospital Med Ct | Hepato-biliary-pancreatic tissues and methods of making same |
| US11426818B2 (en) | 2018-08-10 | 2022-08-30 | The Research Foundation for the State University | Additive manufacturing processes and additively manufactured products |
| CA3112026A1 (en) | 2018-09-12 | 2020-03-19 | Children's Hospital Medical Center | Organoid compositions for the production of hematopoietic stem cells and derivatives thereof |
| US12241049B2 (en) | 2019-01-10 | 2025-03-04 | Brown University | Multi-compartment device containing human three-dimensional (3D) microtissues for toxicity testing |
| MX2021008462A (es) | 2019-01-17 | 2021-08-19 | Modern Meadow Inc | Materiales de colageno estratificados y metodos para fabricarlos. |
| WO2020205567A1 (en) * | 2019-03-29 | 2020-10-08 | Advanced Solutions Life Sciences, Llc | Systems and methods for biomedical object segmentation |
| US12497597B2 (en) | 2019-05-31 | 2025-12-16 | Children's Hospital Medical Center | Methods of generating and expanding hematopoietic stem cells |
| CN113189317B (zh) * | 2021-04-29 | 2024-11-15 | 广东省人民医院 | 一套人工血管三维静态培养的实验装置及其使用方法 |
| AU2022266430A1 (en) * | 2021-04-30 | 2023-12-14 | Riken | Cord-like aggregates of retinal pigment epithelial cells, device and production method for producing same, and therapeutic agent comprising said cord-like aggregates |
| WO2025169815A1 (ja) * | 2024-02-06 | 2025-08-14 | 国立大学法人東北大学 | 分岐人工血管モデルの製造方法 |
| CN119286639B (zh) * | 2024-10-10 | 2025-07-01 | 中南大学湘雅医院 | 一种修复子宫内膜损伤的多细胞组装体安装辅助设备 |
Family Cites Families (113)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4759764A (en) | 1985-05-24 | 1988-07-26 | Clayton Foundation For Research | Peripheral nerve regeneration |
| US4808435A (en) | 1987-04-06 | 1989-02-28 | International Business Machines Corporation | Screen printing method for producing lines of uniform width and height |
| US5099090A (en) | 1988-05-11 | 1992-03-24 | Ariel Electronics, Inc. | Circuit writer |
| US5596251A (en) | 1995-06-07 | 1997-01-21 | Alcoa Closure Systems International, Inc. | Servo motor-driven plastic cutter system for compression molding |
| EP2075015B1 (en) | 1997-07-03 | 2015-03-11 | Massachusetts Institute of Technology | Tissue-engineered constructs |
| US6401795B1 (en) | 1997-10-28 | 2002-06-11 | Sandia Corporation | Method for freeforming objects with low-binder slurry |
| CA2326322C (en) | 1998-04-21 | 2011-03-01 | University Of Connecticut | Free-form nanofabrication using multi-photon excitation |
| US6697694B2 (en) | 1998-08-26 | 2004-02-24 | Electronic Materials, L.L.C. | Apparatus and method for creating flexible circuits |
| US7338798B2 (en) | 1998-09-15 | 2008-03-04 | The Regents Of The University Of Michigan | System and method for forming a cardiac muscle construct |
| EP1029692B9 (en) | 1999-02-17 | 2003-12-03 | Hewlett-Packard Company, A Delaware Corporation | Printing apparatus |
| US6979670B1 (en) * | 1999-03-10 | 2005-12-27 | Biora Bioex Ab | Matrix protein compositions for grafting |
| JP2001145956A (ja) | 1999-11-19 | 2001-05-29 | Meiko:Kk | 光硬化性樹脂三次元造形物の積層造形装置及びその積層造形方法 |
| US6503273B1 (en) * | 1999-11-22 | 2003-01-07 | Cyograft Tissue Engineering, Inc. | Tissue engineered blood vessels and methods and apparatus for their manufacture |
| US6454972B1 (en) | 1999-11-24 | 2002-09-24 | Sandia Corporation | Solid freeform fabrication using chemically reactive suspensions |
| US6520997B1 (en) | 1999-12-08 | 2003-02-18 | Baxter International Inc. | Porous three dimensional structure |
| US6315469B1 (en) | 2000-01-19 | 2001-11-13 | Hewlett-Packard Company | Tool and method for adjustment of printhead to platen spacing in a printer |
| DE10013223C2 (de) | 2000-03-13 | 2002-07-18 | Co Don Ag | Verfahren zur in vitro-Herstellung von dreidimensionalem, vitalem Knorpel- oder Knochengewebe und dessen Verwendung als Transplantationsmaterial |
| US20050091576A1 (en) | 2003-10-24 | 2005-04-28 | Microsoft Corporation | Programming interface for a computer platform |
| DE10018987A1 (de) | 2000-04-17 | 2001-10-31 | Envision Technologies Gmbh | Vorrichtung und Verfahren zum Herstellen von dreidimensionalen Objekten |
| AU2001283492A1 (en) | 2000-07-11 | 2002-01-21 | The Johns Hopkins University School Of Medicine | Methods of patterning protein and cell adhesivity |
| US7998735B2 (en) | 2000-08-21 | 2011-08-16 | Victorian Tissue Engineering Centre Pty. Ltd. | Vascularized tissue graft |
| US6561607B1 (en) | 2000-10-05 | 2003-05-13 | Eastman Kodak Company | Apparatus and method for maintaining a substantially constant closely spaced working distance between an inkjet printhead and a printing receiver |
| US20020129485A1 (en) | 2001-03-13 | 2002-09-19 | Milling Systems And Concepts Pte Ltd | Method and apparatus for producing a prototype |
| US6939489B2 (en) | 2001-03-23 | 2005-09-06 | Ivoclar Vivadent Ag | Desktop process for producing dental products by means of 3-dimensional plotting |
| WO2002085246A2 (en) | 2001-04-19 | 2002-10-31 | Case Western Reserve University | Fabrication of a polymeric prosthetic implant |
| EP1428871B1 (en) | 2001-07-26 | 2008-08-27 | Transparent Inc. | Cultured cell construct containing spheroids of cultured animal cells and utilization thereof |
| US20030049839A1 (en) | 2001-08-01 | 2003-03-13 | The University Of Texas System | Transparent multi-channel cell scaffold that creates a cellular and/or molecular gradient |
| US6986739B2 (en) | 2001-08-23 | 2006-01-17 | Sciperio, Inc. | Architecture tool and methods of use |
| US6934600B2 (en) | 2002-03-14 | 2005-08-23 | Auburn University | Nanotube fiber reinforced composite materials and method of producing fiber reinforced composites |
| AU2003224710A1 (en) | 2002-03-18 | 2003-10-08 | Carnegie Mellon University | Method and apparatus for preparing biomimetic scaffold |
| US20100254900A1 (en) | 2002-03-18 | 2010-10-07 | Campbell Phil G | Biocompatible polymers and Methods of use |
| US6907307B2 (en) | 2002-07-02 | 2005-06-14 | 3D Systems, Inc. | Support volume calculation for a CAD model |
| WO2004006840A2 (en) | 2002-07-12 | 2004-01-22 | The Regents Of The University Of California | Three dimensional cell patterned bioploymer scaffolds and method of making the same |
| US6998264B2 (en) | 2002-11-19 | 2006-02-14 | Huntington Medical Research Institutes | Replication of biological tissue |
| US20050238746A1 (en) | 2002-12-31 | 2005-10-27 | E.I. Dupont De Nemours & Company | Apparatus, system and method for making hydrogel particles |
| US7641898B2 (en) | 2003-03-21 | 2010-01-05 | Materials Evolution And Development Usa, Inc. | Keratinocyte-fibrocyte concomitant grafting for wound healing |
| US20040197375A1 (en) | 2003-04-02 | 2004-10-07 | Alireza Rezania | Composite scaffolds seeded with mammalian cells |
| US7051654B2 (en) | 2003-05-30 | 2006-05-30 | Clemson University | Ink-jet printing of viable cells |
| WO2005003300A2 (en) | 2003-06-04 | 2005-01-13 | University Of South Carolina | Tissue scaffold having aligned fibrils, apparatus and method for producing same, and methods of using same |
| JP4474858B2 (ja) | 2003-07-07 | 2010-06-09 | セイコーエプソン株式会社 | ギャップ調整装置、これに使用されるキャリブレーション用治具、およびギャップ調整装置を備えた液滴吐出装置、並びに電気光学装置の製造方法 |
| US20050074877A1 (en) | 2003-07-28 | 2005-04-07 | Mao Jeremy Jian | Biological engineering of articular structures containing both cartilage and bone |
| FR2859128B1 (fr) | 2003-08-29 | 2006-03-10 | Centre Nat Rech Scient | Procede et dispositif de fabrication d'un composant multimateriaux tridimensionnel par impression du type jet d'encre |
| US8043614B2 (en) | 2004-03-09 | 2011-10-25 | Ahlfors Jan-Eric W | Autogenic living scaffolds and living tissue matrices: methods and uses thereof |
| DE10353281A1 (de) | 2003-11-14 | 2005-06-16 | Bayer Cropscience Ag | Wirkstoffkombination mit insektiziden und akariziden Eigenschaften |
| US20050276791A1 (en) * | 2004-02-20 | 2005-12-15 | The Ohio State University | Multi-layer polymer scaffolds |
| WO2005081970A2 (en) | 2004-02-24 | 2005-09-09 | The Curators Of The University Of Missouri | Self-assembling cell aggregates and methods of making engineered tissue using the same |
| US20090142307A1 (en) * | 2004-07-09 | 2009-06-04 | Athanasiou Kyriacos A | Shape-Based Approach for Scaffoldless Tissue Engineering |
| WO2007115337A2 (en) | 2006-04-05 | 2007-10-11 | William Marsh Rice University | Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering |
| US7939003B2 (en) | 2004-08-11 | 2011-05-10 | Cornell Research Foundation, Inc. | Modular fabrication systems and methods |
| US7651665B2 (en) | 2004-09-07 | 2010-01-26 | Hewlett-Packard Development Company, L.P. | Microtray for handling biosubstances |
| US7767446B2 (en) | 2004-09-16 | 2010-08-03 | Becton, Dickinson And Company | Perfusion bioreactors for culturing cells |
| JP4539316B2 (ja) | 2004-12-08 | 2010-09-08 | セイコーエプソン株式会社 | ヘッド位置補正方法およびヘッド位置補正装置、並びに液滴吐出装置、電気光学装置の製造方法 |
| US7568904B2 (en) | 2005-03-03 | 2009-08-04 | Laser Solutions Co., Ltd. | Stereolithography apparatus |
| JP4729948B2 (ja) | 2005-03-09 | 2011-07-20 | ブラザー工業株式会社 | 液体供給装置、この液体供給装置を備えたインクジェット記録装置 |
| US7780897B2 (en) | 2005-04-22 | 2010-08-24 | Board Of Regents, The University Of Texas System | Hydrogel constructs using stereolithography |
| EP1904625B1 (en) | 2005-05-24 | 2015-04-22 | The Regents of The University of California | Microscale micropatterned engineered in vitro tissue |
| EP2093256A3 (en) | 2005-07-28 | 2009-10-14 | Carnegie Mellon University | Biocompatible polymers and methods of use |
| US8690957B2 (en) | 2005-12-21 | 2014-04-08 | Warsaw Orthopedic, Inc. | Bone graft composition, method and implant |
| US20070200276A1 (en) | 2006-02-24 | 2007-08-30 | Micron Technology, Inc. | Method for rapid printing of near-field and imprint lithographic features |
| JP4919464B2 (ja) * | 2006-03-02 | 2012-04-18 | 国立大学法人大阪大学 | 三次元組織の製造方法およびそれに用いる細胞外マトリックスの製造方法。 |
| US7680555B2 (en) | 2006-04-03 | 2010-03-16 | Stratasys, Inc. | Auto tip calibration in an extrusion apparatus |
| US20070231787A1 (en) | 2006-04-04 | 2007-10-04 | Voelker Mark A | Methods and devices for imaging and manipulating biological samples |
| US20090208466A1 (en) | 2006-04-21 | 2009-08-20 | James Yoo | Ink-jet printing of tissues |
| JP4051077B2 (ja) | 2006-04-28 | 2008-02-20 | シャープ株式会社 | 液滴塗布装置、液滴吐出部のギャップ測定方法、および、液滴吐出部のギャップ調整方法 |
| FR2901143B1 (fr) | 2006-05-16 | 2008-10-03 | Centre Nat Rech Scient | "methode de construction de materiaux vivants fonctionnels, materiaux obtenus et applications" |
| US7959942B2 (en) | 2006-10-20 | 2011-06-14 | Orbusneich Medical, Inc. | Bioabsorbable medical device with coating |
| DE102006053121B3 (de) | 2006-11-10 | 2007-12-27 | Eos Gmbh Electro Optical Systems | Vorrichtung und Verfahren zum Herstellen eines dreidimensionalen Objektes mittels eines Beschichters für pulverförmiges Aufbaumaterial |
| US8764828B2 (en) | 2007-02-02 | 2014-07-01 | The Regents Of The University Of Michigan | System and method for forming bone, ligament, and bone-ligament constructs |
| US8343740B2 (en) | 2007-03-29 | 2013-01-01 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Micro-organ device |
| EP2061587A1 (en) | 2007-04-26 | 2009-05-27 | Sigmoid Pharma Limited | Manufacture of multiple minicapsules |
| AU2008262331B2 (en) | 2007-06-07 | 2013-10-17 | Wake Forest University Health Sciences | Inkjet gene printing |
| EP2664444B1 (en) | 2007-07-25 | 2018-03-28 | Stratasys Ltd. | Solid freeform fabrication using a plurality of modeling materials |
| US20090076531A1 (en) | 2007-09-18 | 2009-03-19 | Richardson Charles L | Method and apparatus for bypass graft |
| CA2701884A1 (en) | 2007-10-15 | 2009-04-23 | Wake Forest University Health Sciences | Methods and compositions for printing biologically compatible nanotube composites of autologous tissue |
| EP2090584A1 (en) | 2008-02-13 | 2009-08-19 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Identification of a novel cysteine-rich cell penetrating peptide |
| KR100922232B1 (ko) | 2008-02-13 | 2009-10-16 | 연세대학교 산학협력단 | 전기수력학 프린팅 기법을 이용한 살아있는 세포의 직접패터닝 방법 및 장치 |
| WO2009102484A2 (en) | 2008-02-14 | 2009-08-20 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
| US20090248145A1 (en) | 2008-03-28 | 2009-10-01 | Nanyang Technological University | Method of forming a three-dimensional structure of unidirectionally aligned cells |
| US8876513B2 (en) | 2008-04-25 | 2014-11-04 | 3D Systems, Inc. | Selective deposition modeling using CW UV LED curing |
| US9303245B2 (en) | 2008-06-20 | 2016-04-05 | Universiteit Maastricht | Self-assembling tissue modules |
| EP2310847B1 (en) | 2008-06-24 | 2016-05-04 | The Curators Of The University Of Missouri | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
| WO2010030964A2 (en) | 2008-09-12 | 2010-03-18 | The Brigham And Women's Hospital, Inc. | 3-dimensional multi-layered hydrogels and methods of making the same |
| US20110250688A1 (en) | 2008-11-24 | 2011-10-13 | Immunotrex Corporation | Three Dimensional Tissue Generation |
| US8691974B2 (en) | 2009-09-28 | 2014-04-08 | Virginia Tech Intellectual Properties, Inc. | Three-dimensional bioprinting of biosynthetic cellulose (BC) implants and scaffolds for tissue engineering |
| US8409603B2 (en) | 2009-10-01 | 2013-04-02 | University Of South Carolina | Compositions and methods for tissue engineering, tissue regeneration and wound healing |
| EP2521587B1 (en) | 2010-01-08 | 2020-04-08 | Wake Forest University Health Sciences | Delivery system |
| US8747880B2 (en) | 2010-02-02 | 2014-06-10 | The Curators Of The University Of Missouri | Engineered biological nerve graft, fabrication and application thereof |
| JP5757961B2 (ja) | 2010-03-04 | 2015-08-05 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | バイオプリンティングステーション、そのバイオプリンティングステーションを含むアセンブリ、およびバイオプリンティング法 |
| GB2478801B (en) | 2010-03-16 | 2012-05-30 | Organovo Inc | Multilayered vascular tubes |
| US9012415B2 (en) | 2010-03-26 | 2015-04-21 | Stemmatters, Biotecnologia E Medicina Regenerativa S.A. | Photo-crosslinked gellan gum-based hydrogels: preparation methods and uses thereof |
| US9592255B2 (en) | 2010-07-01 | 2017-03-14 | The Regents Of The University Of Michigan | Scaffold-free three dimensional nerve fibroblast constructs |
| US20120089238A1 (en) | 2010-10-06 | 2012-04-12 | Hyun-Wook Kang | Integrated organ and tissue printing methods, system and apparatus |
| BR112013009744A2 (pt) | 2010-10-21 | 2016-07-19 | Organovo Inc | dispositivo, sistemas e métodos para a fabricação de tecido |
| HUE029316T2 (en) | 2010-11-04 | 2017-03-28 | Univ Of Pecs | Lung tissue model |
| JP5283747B2 (ja) | 2011-02-09 | 2013-09-04 | 富士フイルム株式会社 | 着色感放射線性組成物、カラーフィルタ、その製造方法、固体撮像素子、及び液晶表示装置 |
| DE202011003443U1 (de) | 2011-03-02 | 2011-12-23 | Bego Medical Gmbh | Vorrichtung zur generativen Herstellung dreidimensionaler Bauteile |
| CA2828705A1 (en) | 2011-03-07 | 2012-09-13 | Wake Forest University Health Sciences | Delivery system |
| JP5599356B2 (ja) | 2011-03-31 | 2014-10-01 | 富士フイルム株式会社 | シミュレーション方法、プログラムおよびそれを記録した記録媒体、並びに、それらを利用した液滴配置パターンの作成方法、ナノインプリント方法、パターン化基板の製造方法およびインクジェット装置。 |
| AU2012308724A1 (en) | 2011-09-12 | 2014-04-24 | Organovo, Inc. | Platform for engineered implantable tissues and organs and methods of making the same |
| HK1200483A1 (en) | 2011-09-12 | 2015-08-07 | 奥加诺沃公司 | Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same |
| WO2013116443A1 (en) | 2012-02-01 | 2013-08-08 | Nscrypt, Inc. | Micro-dispensing multi-layered 3d objects with curing steps |
| US10072257B2 (en) | 2012-02-29 | 2018-09-11 | Massachusetts Institute Of Technology | Inverse patterning process for three-dimensional multi-compartmental micro-organization of multiple cell types |
| CA2866267A1 (en) | 2012-03-06 | 2013-09-12 | The Uab Research Foundation | Three-dimesional, prevascularized, engineered tissue constructs, methods of making and methods of using the tissue constructs |
| US9499779B2 (en) | 2012-04-20 | 2016-11-22 | Organovo, Inc. | Devices, systems, and methods for the fabrication of tissue utilizing UV cross-linking |
| US20140099709A1 (en) | 2012-06-19 | 2014-04-10 | Organovo, Inc. | Engineered three-dimensional connective tissue constructs and methods of making the same |
| US9399322B2 (en) | 2012-08-08 | 2016-07-26 | Makerbot Industries, Llc | Three dimensional printer with removable, replaceable print nozzle |
| US9442105B2 (en) | 2013-03-15 | 2016-09-13 | Organovo, Inc. | Engineered liver tissues, arrays thereof, and methods of making the same |
| CN105188993A (zh) | 2013-03-15 | 2015-12-23 | 麦特法布公司 | 用于增材制造装置的料盒和方法 |
| US9802360B2 (en) | 2013-06-04 | 2017-10-31 | Stratsys, Inc. | Platen planarizing process for additive manufacturing system |
| AU2014296246B2 (en) | 2013-07-31 | 2021-01-21 | Organovo, Inc. | Automated devices, systems, and methods for the fabrication of tissue |
| AU2014389440A1 (en) | 2014-04-04 | 2016-10-20 | Oregon Health & Science University | Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model |
| MA40598A (fr) | 2014-11-05 | 2017-09-13 | Oreal | Tissus cutanés tridimensionnels manipulés, ensembles correspondants et leurs procédés de production |
| US20170130192A1 (en) | 2015-11-09 | 2017-05-11 | Organovo, Inc. | Methods for tissue fabrication |
-
2009
- 2009-06-24 EP EP09798534.5A patent/EP2310847B1/en not_active Not-in-force
- 2009-06-24 US US12/491,228 patent/US8143055B2/en active Active
- 2009-06-24 KR KR1020167009102A patent/KR101711354B1/ko not_active Expired - Fee Related
- 2009-06-24 JP JP2011516626A patent/JP5624981B2/ja not_active Expired - Fee Related
- 2009-06-24 AU AU2009271223A patent/AU2009271223B2/en not_active Ceased
- 2009-06-24 CA CA2729559A patent/CA2729559C/en not_active Expired - Fee Related
- 2009-06-24 WO PCT/US2009/048530 patent/WO2010008905A2/en not_active Ceased
- 2009-06-24 CN CN2009801319242A patent/CN102124331B/zh not_active Expired - Fee Related
- 2009-06-24 KR KR1020177004327A patent/KR20170020942A/ko not_active Ceased
- 2009-06-24 KR KR1020157027110A patent/KR101612049B1/ko not_active Expired - Fee Related
- 2009-06-24 KR KR1020117001559A patent/KR101611936B1/ko not_active Expired - Fee Related
- 2009-06-24 CA CA2949615A patent/CA2949615A1/en not_active Abandoned
- 2009-06-24 EP EP16162431.7A patent/EP3059302A1/en not_active Withdrawn
-
2012
- 2012-02-22 US US13/402,215 patent/US8728807B2/en active Active
-
2014
- 2014-04-03 US US14/244,679 patent/US9556415B2/en active Active
- 2014-09-29 JP JP2014198119A patent/JP5985570B2/ja not_active Expired - Fee Related
-
2016
- 2016-08-03 JP JP2016153049A patent/JP2016187359A/ja active Pending
-
2017
- 2017-01-18 US US15/409,034 patent/US20170152474A1/en not_active Abandoned
-
2019
- 2019-12-19 US US16/720,939 patent/US11518978B2/en active Active
-
2022
- 2022-10-31 US US18/051,215 patent/US20230220330A1/en not_active Abandoned
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20230220330A1 (en) | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same | |
| JP6712220B2 (ja) | 包埋された脈管構造を有する組織構成物をプリントする方法 | |
| US8747880B2 (en) | Engineered biological nerve graft, fabrication and application thereof | |
| RU2623303C2 (ru) | Платформа для инженерии имплантируемых тканей и органов и способы создания (биофабрикации) этих тканей и органов | |
| HK1157440B (en) | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20210625 |