CA2729160C - A chimeric bacteriophage lysin with activity against staphylococci bacteria - Google Patents

A chimeric bacteriophage lysin with activity against staphylococci bacteria Download PDF

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Publication number
CA2729160C
CA2729160C CA2729160A CA2729160A CA2729160C CA 2729160 C CA2729160 C CA 2729160C CA 2729160 A CA2729160 A CA 2729160A CA 2729160 A CA2729160 A CA 2729160A CA 2729160 C CA2729160 C CA 2729160C
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CA
Canada
Prior art keywords
lysin
chimeric
bacteriophage lysin
chimeric bacteriophage
clys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2729160A
Other languages
English (en)
French (fr)
Other versions
CA2729160A1 (en
Inventor
Vincent A. Fischetti
Anu Daniel
Chad Euler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rockefeller University
Original Assignee
Rockefeller University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rockefeller University filed Critical Rockefeller University
Priority to CA2984933A priority Critical patent/CA2984933C/en
Publication of CA2729160A1 publication Critical patent/CA2729160A1/en
Application granted granted Critical
Publication of CA2729160C publication Critical patent/CA2729160C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
CA2729160A 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria Active CA2729160C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2984933A CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7827708P 2008-07-03 2008-07-03
US61/078,277 2008-07-03
PCT/US2009/049349 WO2010002959A2 (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2984933A Division CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Publications (2)

Publication Number Publication Date
CA2729160A1 CA2729160A1 (en) 2010-01-07
CA2729160C true CA2729160C (en) 2017-11-14

Family

ID=41466590

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2729160A Active CA2729160C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria
CA2984933A Active CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2984933A Active CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Country Status (8)

Country Link
US (4) US8840900B2 (https=)
EP (2) EP2307557B1 (https=)
JP (2) JP5758291B2 (https=)
AU (2) AU2009266982B2 (https=)
CA (2) CA2729160C (https=)
ES (1) ES2627321T3 (https=)
HK (1) HK1248722A1 (https=)
WO (1) WO2010002959A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8389469B2 (en) 2005-06-06 2013-03-05 The Rockefeller University Bacteriophage lysins for Bacillus anthracis
EP2360248A1 (en) 2005-08-24 2011-08-24 The Rockefeller University Ply-GBS mutant lysins
DK2699253T3 (en) 2011-04-21 2018-07-16 Univ Rockefeller STREPTOCOCCUS BACTERYPHAGIC LYSINES FOR THE DETECTION AND TREATMENT OF GRAM POSITIVE BACTERIES
DK3085777T3 (da) * 2011-05-04 2020-02-03 Micreos Human Health Bv Polypeptid
US9279118B2 (en) 2011-10-05 2016-03-08 The Rockefeller University Dimeric bacteriophage lysins
RU2018121290A (ru) 2012-05-09 2019-03-06 Контрафект Корпорейшн Комбинации лизина бактериофага и антибиотика против грам-положительных бактерий
BR112014027818B1 (pt) * 2012-05-09 2023-03-28 Contrafect Corporation Usos de composições compreendendo polipeptídeo de lisina para prevenção, ruptura ou tratamento de um biofilme bacteriano
CN103122347B (zh) * 2013-02-01 2015-04-22 中国科学院武汉病毒研究所 一种杀灭葡萄球菌的裂解酶及其应用
ES2525954B1 (es) * 2013-05-28 2015-10-23 Consejo Superior De Investigaciones Científicas (Csic) Enzibióticos bactericidas mejorados frente a neumococo y otras bacterias
CN104762285B (zh) * 2015-04-23 2017-10-27 武汉菲吉乐科生物科技有限公司 一种自内裂解大肠杆菌的裂解酶及其应用
CN104805066B (zh) * 2015-05-18 2018-04-24 武汉菲吉乐科生物科技有限公司 一种葡萄球菌裂解酶及其应用
EP3454888B1 (en) 2016-05-12 2021-02-24 Contrafect Corporation Broth microdilution method for evaluating and determining minimal inhibitory concentration of antibacterial polypeptides
BR112020026267A2 (pt) * 2018-06-22 2021-04-06 Contrafect Corporation Lisinas e seus derivados resensibilizam novamente staphylococcus aureus e bactérias gram-positivas aos antibióticos
WO2020032232A1 (ja) * 2018-08-10 2020-02-13 Ｍｅｉｊｉ Ｓｅｉｋａファルマ株式会社 バクテリオファージ剤
CN109666667A (zh) * 2019-01-22 2019-04-23 上海交通大学 杀灭金黄色葡萄球菌的嵌合酶抗生素及其制备和应用
WO2021084558A2 (en) * 2019-10-31 2021-05-06 Bactoclear Holdings Pte. Ltd Chimeric proteins for selective lysis of bacteria
WO2021178968A1 (en) * 2020-03-06 2021-09-10 Endolytix Technology, Inc. Compositions and methods for the treatment of intracellular bacterial infections
WO2021180892A1 (en) * 2020-03-11 2021-09-16 Telum Therapeutics S.L. New recombinant lysin and its use in the treatment of gram-negative bacterial infections
CN111808837A (zh) * 2020-07-17 2020-10-23 青岛诺安百特生物技术有限公司 一种金黄色葡萄球菌噬菌体裂解酶及其制备方法和应用
CN115820616A (zh) * 2022-07-22 2023-03-21 昆明理工大学 一种带荧光标记的噬菌体裂解酶及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008553A1 (en) * 1995-08-22 1997-03-06 The Regents Of The University Of California Targeting of proteins to the cell wall of gram-positive bacteria
US6056954A (en) * 1997-10-31 2000-05-02 New Horizons Diagnostics Corp Use of bacterial phage associated lysing enzymers for the prophylactic and therapeutic treatment of various illnesses
US6703025B1 (en) * 1998-08-31 2004-03-09 Inhibitex, Inc. Multicomponent vaccines
US20020127215A1 (en) * 1999-09-14 2002-09-12 Lawrence Loomis Parenteral use of bacterial phage associated lysing enzymes for the therapeutic treatment of bacterial infections
GB0107661D0 (en) * 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
US20040213765A1 (en) * 2001-07-13 2004-10-28 Vincent Fischetti Use of bacterial phage associated lytic enzymes to prevent food poisoning
EP1504088B1 (en) * 2002-03-25 2007-08-15 University Of Warwick Bacteriophages useful for therapy and prophylaxis of bacterial infections
JPWO2004020635A1 (ja) * 2002-08-28 2005-12-15 株式会社テクノネットワーク四国 バクテリオファージおよび細菌感染症治療剤
EP2360248A1 (en) * 2005-08-24 2011-08-24 The Rockefeller University Ply-GBS mutant lysins
WO2009024327A2 (en) 2007-08-22 2009-02-26 Profos Ag New proteins for use in human and animal staphylococcus infections
US8563504B2 (en) * 2008-10-10 2013-10-22 Lusomedicamenta, S.A. Antibacterial phage peptides and methods of use thereof
AU2010211456B2 (en) * 2009-02-06 2016-05-26 Technophage, Investigação E Desenvolvimento Em Biotecnologia, S.A. Antibacterial phage, phage peptides and methods of use thereof

Also Published As

Publication number Publication date
AU2009266982B2 (en) 2015-06-25
EP2307557A4 (en) 2012-04-11
US8840900B2 (en) 2014-09-23
AU2009266982A1 (en) 2010-01-07
AU2015230807B2 (en) 2017-07-27
JP5758291B2 (ja) 2015-08-05
CA2729160A1 (en) 2010-01-07
US11015182B2 (en) 2021-05-25
WO2010002959A2 (en) 2010-01-07
EP3263588A1 (en) 2018-01-03
US20190055533A1 (en) 2019-02-21
JP2011526920A (ja) 2011-10-20
US10053681B2 (en) 2018-08-21
US20150064156A1 (en) 2015-03-05
ES2627321T3 (es) 2017-07-27
JP2015126738A (ja) 2015-07-09
US20120258088A1 (en) 2012-10-11
JP6169632B2 (ja) 2017-07-26
CA2984933C (en) 2020-07-07
US11827911B2 (en) 2023-11-28
EP2307557B1 (en) 2017-03-01
HK1248722A1 (en) 2018-10-19
WO2010002959A3 (en) 2010-05-06
AU2015230807A1 (en) 2015-10-15
CA2984933A1 (en) 2010-01-07
EP2307557A2 (en) 2011-04-13
US20210348146A1 (en) 2021-11-11

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