CA2719549A1 - Droplet dispensing device and methods - Google Patents
Droplet dispensing device and methods Download PDFInfo
- Publication number
- CA2719549A1 CA2719549A1 CA2719549A CA2719549A CA2719549A1 CA 2719549 A1 CA2719549 A1 CA 2719549A1 CA 2719549 A CA2719549 A CA 2719549A CA 2719549 A CA2719549 A CA 2719549A CA 2719549 A1 CA2719549 A1 CA 2719549A1
- Authority
- CA
- Canada
- Prior art keywords
- droplet
- electrode
- electrodes
- reservoir
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 316
- 238000000203 droplet dispensing Methods 0.000 title description 176
- 239000012530 fluid Substances 0.000 claims abstract description 329
- 239000000758 substrate Substances 0.000 claims description 104
- 239000011324 bead Substances 0.000 claims description 84
- 230000005291 magnetic effect Effects 0.000 claims description 74
- 230000003213 activating effect Effects 0.000 claims description 28
- 238000012546 transfer Methods 0.000 claims description 21
- 238000004891 communication Methods 0.000 claims description 20
- 230000004888 barrier function Effects 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 13
- 230000004913 activation Effects 0.000 claims description 11
- 230000009849 deactivation Effects 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- 238000003491 array Methods 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 238000001311 chemical methods and process Methods 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 1
- 229910000595 mu-metal Inorganic materials 0.000 claims 1
- 239000007788 liquid Substances 0.000 description 89
- 230000008569 process Effects 0.000 description 56
- 239000002585 base Substances 0.000 description 11
- 239000000945 filler Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
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- 238000005406 washing Methods 0.000 description 5
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- 238000013019 agitation Methods 0.000 description 2
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- QDOXWKRWXJOMAK-UHFFFAOYSA-N dichromium trioxide Chemical compound O=[Cr]O[Cr]=O QDOXWKRWXJOMAK-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000000416 exudates and transudate Anatomy 0.000 description 2
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- 206010036790 Productive cough Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
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- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 238000001690 micro-dialysis Methods 0.000 description 1
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- 239000002102 nanobead Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 210000004912 pericardial fluid Anatomy 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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- 239000000565 sealant Substances 0.000 description 1
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- 210000002966 serum Anatomy 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
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- 210000002700 urine Anatomy 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
- B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/088—Channel loops
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/089—Virtual walls for guiding liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0427—Electrowetting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Fluid Mechanics (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Coating Apparatus (AREA)
- Micromachines (AREA)
- Jet Pumps And Other Pumps (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Application Of Or Painting With Fluid Materials (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US91089707P | 2007-04-10 | 2007-04-10 | |
| US60/910,897 | 2007-04-10 | ||
| US98020207P | 2007-10-16 | 2007-10-16 | |
| US60/980,202 | 2007-10-16 | ||
| PCT/US2008/059955 WO2008124846A2 (en) | 2007-04-10 | 2008-04-10 | Droplet dispensing device and methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2719549A1 true CA2719549A1 (en) | 2008-10-16 |
Family
ID=39831590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2719549A Abandoned CA2719549A1 (en) | 2007-04-10 | 2008-04-10 | Droplet dispensing device and methods |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20100032293A1 (enExample) |
| EP (1) | EP2132296A4 (enExample) |
| JP (1) | JP2010524002A (enExample) |
| KR (1) | KR20100016343A (enExample) |
| CN (1) | CN101743304B (enExample) |
| AU (1) | AU2008237017B2 (enExample) |
| BR (1) | BRPI0809978A2 (enExample) |
| CA (1) | CA2719549A1 (enExample) |
| WO (1) | WO2008124846A2 (enExample) |
Families Citing this family (91)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2594483C (en) | 2005-01-28 | 2014-08-26 | Duke University | Apparatuses and methods for manipulating droplets on a printed circuit board |
| US20140193807A1 (en) | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
| US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
| US8809068B2 (en) | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
| US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
| US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
| US8716015B2 (en) | 2006-04-18 | 2014-05-06 | Advanced Liquid Logic, Inc. | Manipulation of cells on a droplet actuator |
| US8658111B2 (en) * | 2006-04-18 | 2014-02-25 | Advanced Liquid Logic, Inc. | Droplet actuators, modified fluids and methods |
| US9675972B2 (en) | 2006-05-09 | 2017-06-13 | Advanced Liquid Logic, Inc. | Method of concentrating beads in a droplet |
| US8685344B2 (en) | 2007-01-22 | 2014-04-01 | Advanced Liquid Logic, Inc. | Surface assisted fluid loading and droplet dispensing |
| EP2111554B1 (en) | 2007-02-09 | 2013-05-08 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
| EP2109774B1 (en) | 2007-02-15 | 2018-07-04 | Advanced Liquid Logic, Inc. | Capacitance detection in a droplet actuator |
| US8951732B2 (en) | 2007-06-22 | 2015-02-10 | Advanced Liquid Logic, Inc. | Droplet-based nucleic acid amplification in a temperature gradient |
| MX2010002079A (es) * | 2007-08-24 | 2010-08-09 | Advanced Liquid Logic Inc | Manipulacion de esferas en un inyector de gotas. |
| WO2009032863A2 (en) | 2007-09-04 | 2009-03-12 | Advanced Liquid Logic, Inc. | Droplet actuator with improved top substrate |
| WO2009052123A2 (en) * | 2007-10-17 | 2009-04-23 | Advanced Liquid Logic, Inc. | Multiplexed detection schemes for a droplet actuator |
| AU2008345138B2 (en) | 2007-12-23 | 2014-05-29 | Advanced Liquid Logic, Inc. | Droplet actuator configurations and methods of conducting droplet operations |
| US8852952B2 (en) | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
| EP2286228B1 (en) * | 2008-05-16 | 2019-04-03 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods for manipulating beads |
| US8877512B2 (en) | 2009-01-23 | 2014-11-04 | Advanced Liquid Logic, Inc. | Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator |
| US8926065B2 (en) | 2009-08-14 | 2015-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
| FR2950544B1 (fr) * | 2009-09-29 | 2011-12-09 | Ecole Polytech | Circuit microfluidique |
| US9091649B2 (en) | 2009-11-06 | 2015-07-28 | Advanced Liquid Logic, Inc. | Integrated droplet actuator for gel; electrophoresis and molecular analysis |
| EP2516669B1 (en) | 2009-12-21 | 2016-10-12 | Advanced Liquid Logic, Inc. | Enzyme assays on a droplet actuator |
| US8834695B2 (en) * | 2010-03-09 | 2014-09-16 | Sparkle Power Inc. | Droplet manipulations on EWOD microelectrode array architecture |
| EP2553473A4 (en) | 2010-03-30 | 2016-08-10 | Advanced Liquid Logic Inc | DROPLET OPERATION PLATFORM |
| CA2798123C (en) | 2010-05-05 | 2020-06-23 | The Governing Council Of The University Of Toronto | Method of processing dried samples using digital microfluidic device |
| US9011662B2 (en) | 2010-06-30 | 2015-04-21 | Advanced Liquid Logic, Inc. | Droplet actuator assemblies and methods of making same |
| EP2711079B1 (en) | 2011-05-09 | 2018-12-19 | Advanced Liquid Logic, Inc. | Microfluidic Feedback Using Impedance Detection |
| CN103597356A (zh) | 2011-05-10 | 2014-02-19 | 先进流体逻辑公司 | 酶浓缩与分析 |
| US8901043B2 (en) | 2011-07-06 | 2014-12-02 | Advanced Liquid Logic, Inc. | Systems for and methods of hybrid pyrosequencing |
| KR20140064771A (ko) | 2011-07-06 | 2014-05-28 | 어드밴스드 리퀴드 로직, 아이엔씨. | 비말 작동기 상의 시약 저장 |
| WO2013009927A2 (en) | 2011-07-11 | 2013-01-17 | Advanced Liquid Logic, Inc. | Droplet actuators and techniques for droplet-based assays |
| WO2013016413A2 (en) | 2011-07-25 | 2013-01-31 | Advanced Liquid Logic Inc | Droplet actuator apparatus and system |
| US8637242B2 (en) | 2011-11-07 | 2014-01-28 | Illumina, Inc. | Integrated sequencing apparatuses and methods of use |
| WO2013078216A1 (en) | 2011-11-21 | 2013-05-30 | Advanced Liquid Logic Inc | Glucose-6-phosphate dehydrogenase assays |
| US8808519B2 (en) * | 2011-12-14 | 2014-08-19 | Hamilton Sundstrand Space Systems International | Microfluidic device |
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-
2008
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- 2008-04-10 KR KR1020097023326A patent/KR20100016343A/ko not_active Ceased
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| AU2008237017B2 (en) | 2013-10-24 |
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| US20150075985A1 (en) | 2015-03-19 |
| US20100032293A1 (en) | 2010-02-11 |
| JP2010524002A (ja) | 2010-07-15 |
| CN101743304A (zh) | 2010-06-16 |
| AU2008237017A1 (en) | 2008-10-16 |
| EP2132296A4 (en) | 2015-04-08 |
| WO2008124846A3 (en) | 2009-12-23 |
| CN101743304B (zh) | 2013-04-24 |
| WO2008124846A2 (en) | 2008-10-16 |
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