CA2711373C - Isolement et enumeration de cellules a partir d'une matrice d'echantillon complexe - Google Patents

Isolement et enumeration de cellules a partir d'une matrice d'echantillon complexe Download PDF

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Publication number
CA2711373C
CA2711373C CA2711373A CA2711373A CA2711373C CA 2711373 C CA2711373 C CA 2711373C CA 2711373 A CA2711373 A CA 2711373A CA 2711373 A CA2711373 A CA 2711373A CA 2711373 C CA2711373 C CA 2711373C
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Prior art keywords
sample
chamber
cells
cell
capture complex
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CA2711373A
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CA2711373A1 (fr
Inventor
Bruce Bernard
John Carrano
Amy Altman
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Luminex Corp
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Luminex Corp
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention porte sur des procédés et des systèmes pour marquer, isoler, détecter et dénombrer des cellules biologiques ou d'autres analytes biologiques d'intérêt présents dans un échantillon, le complexe de capture pouvant également servir d'agent de marquage. Dans un mode de réalisation, le complexe de capture est une bille magnétique codée couplée à des anticorps présentant une affinité spécifique pour une protéine de surface cellulaire sur une cellule d'intérêt. Les procédés et les systèmes de la présente invention peuvent être utilisés pour une détection quantitative ou qualitative.
CA2711373A 2008-01-07 2009-01-07 Isolement et enumeration de cellules a partir d'une matrice d'echantillon complexe Active CA2711373C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1948508P 2008-01-07 2008-01-07
US61/019,485 2008-01-07
PCT/US2009/030274 WO2009091643A1 (fr) 2008-01-07 2009-01-07 Isolement et énumération de cellules à partir d'une matrice d'échantillon complexe

Publications (2)

Publication Number Publication Date
CA2711373A1 CA2711373A1 (fr) 2009-07-23
CA2711373C true CA2711373C (fr) 2014-10-28

Family

ID=40677500

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2711373A Active CA2711373C (fr) 2008-01-07 2009-01-07 Isolement et enumeration de cellules a partir d'une matrice d'echantillon complexe

Country Status (6)

Country Link
US (1) US8372590B2 (fr)
EP (1) EP2240778B1 (fr)
JP (1) JP5550182B2 (fr)
CN (1) CN101939649B (fr)
CA (1) CA2711373C (fr)
WO (1) WO2009091643A1 (fr)

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US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
CN103026230B (zh) * 2010-07-23 2015-01-14 卢米耐克斯公司 共偶联以控制免疫测定中试剂的反应性
KR101381604B1 (ko) 2010-08-31 2014-04-11 한국전자통신연구원 고분자 박막 및 자기비드를 이용한 바이오 물질 측정방법
WO2012148648A2 (fr) 2011-04-27 2012-11-01 Becton Dickinson & Co. Dispositifs et procédés de séparation de fragments marqués magnétiquement dans un échantillon
GB201202762D0 (en) 2012-02-17 2012-04-04 Autology Health Ltd High efficiency cell capture
CN102841058A (zh) * 2012-08-30 2012-12-26 山西华诚睿光生物科技有限公司 牛奶中体细胞数的检测方法
CN104736718A (zh) 2012-10-26 2015-06-24 贝克顿·迪金森公司 用于操纵流体样品中的组分的装置和方法
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
CN103293282B (zh) * 2013-06-05 2015-10-28 南昌大学 快速分离铜绿假单胞菌的方法
CN103275904B (zh) * 2013-06-05 2015-08-12 南昌大学 肺炎链球菌在复杂基质中的分离方法
FR3027396B1 (fr) * 2014-10-15 2016-11-25 Espci Innov Procede d'analyse du contenu de gouttes et appareil associe
US12339233B2 (en) 2015-12-24 2025-06-24 Siemens Healthineers Nederland B.V. Optical detection of a substance in fluid
CN107192818B (zh) * 2017-05-23 2018-06-29 重庆天之助生物科技有限公司 一种微粒子色度聚类分析方法及试剂盒
US11807898B2 (en) 2017-08-14 2023-11-07 The Governing Council Of The University Of Toronto Method for determination of cellular mRNA
US12194157B2 (en) 2020-04-09 2025-01-14 Finncure Oy Carrier for targeted delivery to a host
FI20215508A1 (en) 2020-04-09 2021-10-10 Niemelae Erik Johan Mimetic nanoparticles to prevent the spread of new coronaviruses and reduce the rate of infection
CN116731962B (zh) * 2023-08-14 2023-11-03 天津中新科炬生物制药股份有限公司 全血中分离红细胞的试剂盒及方法
CN117218139B (zh) * 2023-09-12 2024-05-24 珠海横琴圣澳云智科技有限公司 样本细胞密度的确定方法和装置

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Also Published As

Publication number Publication date
CN101939649B (zh) 2013-10-23
CA2711373A1 (fr) 2009-07-23
JP2011508892A (ja) 2011-03-17
JP5550182B2 (ja) 2014-07-16
US8372590B2 (en) 2013-02-12
HK1151855A1 (en) 2012-02-10
EP2240778B1 (fr) 2014-07-23
EP2240778A1 (fr) 2010-10-20
CN101939649A (zh) 2011-01-05
WO2009091643A1 (fr) 2009-07-23
US20090246796A1 (en) 2009-10-01

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