CA2697649C - Proton concentration topographies, methods and devices for producing the same - Google Patents

Proton concentration topographies, methods and devices for producing the same Download PDF

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Publication number
CA2697649C
CA2697649C CA2697649A CA2697649A CA2697649C CA 2697649 C CA2697649 C CA 2697649C CA 2697649 A CA2697649 A CA 2697649A CA 2697649 A CA2697649 A CA 2697649A CA 2697649 C CA2697649 C CA 2697649C
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Canada
Prior art keywords
proton concentration
volume
environment
proton
topography
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CA2697649A
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English (en)
French (fr)
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CA2697649A1 (en
Inventor
Uri Sivan
Elad Brod
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Technion Research and Development Foundation Ltd
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Technion Research and Development Foundation Ltd
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Publication of CA2697649A1 publication Critical patent/CA2697649A1/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
    • C02F1/46104—Devices therefor; Their operating or servicing
    • C02F1/46109—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
    • C02F1/46104—Devices therefor; Their operating or servicing
    • C02F1/4618—Devices therefor; Their operating or servicing for producing "ionised" acidic or basic water
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
    • C02F1/46104—Devices therefor; Their operating or servicing
    • C02F1/46109—Electrodes
    • C02F2001/46152—Electrodes characterised by the shape or form
    • C02F2001/46157—Perforated or foraminous electrodes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/46115—Electrolytic cell with membranes or diaphragms
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/4612—Controlling or monitoring
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/4612—Controlling or monitoring
    • C02F2201/46125—Electrical variables
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/4612—Controlling or monitoring
    • C02F2201/46125—Electrical variables
    • C02F2201/4614—Current
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Electrolytic Production Of Non-Metals, Compounds, Apparatuses Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA2697649A 2007-08-27 2008-08-26 Proton concentration topographies, methods and devices for producing the same Active CA2697649C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US93569807P 2007-08-27 2007-08-27
US60/935,698 2007-08-27
US3925708P 2008-03-25 2008-03-25
US61/039,257 2008-03-25
PCT/IL2008/001159 WO2009027970A2 (en) 2007-08-27 2008-08-26 Ph gradients controlled by electrolysis, and their use in isoelectric focusing

Publications (2)

Publication Number Publication Date
CA2697649A1 CA2697649A1 (en) 2009-03-05
CA2697649C true CA2697649C (en) 2016-08-09

Family

ID=40254551

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2697649A Active CA2697649C (en) 2007-08-27 2008-08-26 Proton concentration topographies, methods and devices for producing the same

Country Status (8)

Country Link
US (2) US9274082B2 (https=)
EP (2) EP2193100B1 (https=)
JP (1) JP5702142B2 (https=)
KR (1) KR20100053672A (https=)
CN (2) CN103134843B (https=)
AU (1) AU2008293381B2 (https=)
CA (1) CA2697649C (https=)
WO (1) WO2009027970A2 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008293381B2 (en) 2007-08-27 2012-07-19 Technion Research & Development Foundation Ltd. pH gradients controlled by electrolysis, and their use in isoelectric focusing
US10107782B2 (en) * 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
CN102472724B (zh) * 2009-08-18 2014-08-13 工业研究与发展基金会有限公司 分离分子分析物的方法和装置
EP2467709B1 (en) 2009-08-18 2018-02-28 Technion Research & Development Foundation Ltd. Proton concentration topographies, methods and devices for producing the same
JP5950429B2 (ja) * 2011-05-13 2016-07-13 シャープ株式会社 電気泳動方法、及び電気泳動装置
CN104024396A (zh) 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 采用pH的电子控制的亲和方法及组合物
EP2773959B1 (en) * 2011-11-04 2019-04-10 Bio-Rad Laboratories, Inc. Protein fractionation based on isoelectric focusing
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2773221B1 (en) 2011-11-04 2019-01-09 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
CN102565171A (zh) * 2012-01-05 2012-07-11 厦门大学 一种用于等电聚焦分离的微流控芯片
EP2814597A4 (en) 2012-02-15 2015-10-28 Bio Rad Laboratories ELECTRONIC CONTROL OF PH-VALUE AND ION STRENGTH
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
CN104034789A (zh) * 2013-04-02 2014-09-10 上海交通大学 蛋白质等电聚焦电泳的装置
CN106267304B (zh) * 2015-06-09 2019-10-18 海南光宇生物科技有限公司 具有酸碱性的生物纤维素凝胶膜及由其制备的医用敷料
CN106260992B (zh) * 2015-06-09 2020-02-18 海南椰国食品有限公司 一种含有酸性椰果和/或碱性椰果的食品
EP3653121A1 (en) * 2018-11-14 2020-05-20 Koninklijke Philips N.V. Sensor unit, body fluid monitoring device and method for detecting an analyte
CN119470588A (zh) * 2024-11-18 2025-02-18 中国科学院空天信息创新研究院 集成式多种重金属检测微传感器

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987001206A1 (en) * 1985-08-21 1987-02-26 William Rudolph Hargreaves Methods and devices for separating, mixing, and detecting components in specific binding assays
US4670119A (en) * 1985-10-25 1987-06-02 Hurd Stanley M Isoelectric focusing device and process
US4900414A (en) * 1988-08-19 1990-02-13 Drug Delivery Systems Inc. Commercial separation system and method using electrokinetic techniques
US5160594A (en) 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
DE3929137C1 (https=) * 1989-09-01 1991-02-28 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung Ev, 8000 Muenchen, De
US5110434A (en) * 1990-12-20 1992-05-05 Bio-Rad Laboratories, Inc. Use of zwitterions to mobilize isoelectrically focused ampholyte zones
US5646001A (en) * 1991-03-25 1997-07-08 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
GB2275974B (en) 1993-03-10 1996-01-10 Curty Payen Sa Improvements in and relating to gaskets
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
JPH0933479A (ja) * 1995-07-18 1997-02-07 Mizu Kk センサカバー及びこれを用いた電解水生成装置
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
AU4546899A (en) * 1998-06-05 1999-12-20 Sarnoff Corporation Apparatus for separating molecules
JP3113645B2 (ja) * 1999-03-01 2000-12-04 ファースト・オーシャン株式会社 電解水製造法
AU4773400A (en) * 1999-05-28 2000-12-18 Leisamon (Israel), Ltd. Methods and apparatus for nonlinear mobility electrophoresis separation
DE10047088C2 (de) 2000-09-21 2002-10-17 Gerhard Weber Medium für analytische und präparative Elektrophorese
JP2002265494A (ja) 2001-03-06 2002-09-18 Tokuyama Corp 混合物から高純度化された目的物を製造する方法
CN1549924A (zh) * 2001-07-16 2004-11-24 �����ʸ���˹�ع�˾ 通过等电点分析生物分子的缓冲液的阵列
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
SE0203773D0 (sv) * 2002-12-19 2002-12-19 Capture Device Ab Method and device for capturing charged molecules traveling in a flow stream
US7150813B2 (en) * 2003-06-12 2006-12-19 Palo Alto Research Center Incorporated Isoelectric focusing (IEF) of proteins with sequential and oppositely directed traveling waves in gel electrophoresis
JP2007504215A (ja) 2003-09-03 2007-03-01 バイオフォームズ 生体分子を急速に結晶化するための方法及び装置
JP2006213932A (ja) 2004-05-07 2006-08-17 Honda Motor Co Ltd 電解水生成用電極
CN1258828C (zh) 2004-02-13 2006-06-07 清华大学 一种质子交换膜燃料电池多元催化剂的制备方法
US7238272B2 (en) * 2004-02-27 2007-07-03 Yoichi Sano Production of electrolytic water
EP1776581B1 (en) * 2004-07-19 2015-05-06 ProteinSimple Method for protein detection
US7749370B2 (en) * 2005-02-03 2010-07-06 Osao Sumita Manufacturing method of oxidative water to be employed for sterilization
JP5129442B2 (ja) 2005-02-08 2013-01-30 エア・ウォーター株式会社 液体の帯電物質の濃度調節装置
JP4856530B2 (ja) 2005-12-21 2012-01-18 ミドリ安全株式会社 電解水の有効塩素濃度調節方法、電解水のｐＨ調節方法および電解水生成装置。
US20090008268A1 (en) 2006-02-17 2009-01-08 Peter Salathe Process for Production of a Disinfectant Through the Electrochemical Activation (Eca) of Water, a Disinfectant Produced in this Way and the Use Thereof
WO2008112253A1 (en) 2007-03-13 2008-09-18 Siemens Water Technologies Corp. Devices and methods for acid and base generation
US20110100821A1 (en) 2007-04-20 2011-05-05 Brian Furmanski Mineral oil free isoelectric focusing apparatus for immobilized ph gradient strips
CN101294930B (zh) 2007-04-27 2013-08-14 杨春 一种整体型固定化pH梯度的制备方法及其应用
WO2009002459A2 (en) 2007-06-22 2008-12-31 Massachusetts Institute Of Technology Free flow isoelectric focusing devices and methods
AU2008293381B2 (en) 2007-08-27 2012-07-19 Technion Research & Development Foundation Ltd. pH gradients controlled by electrolysis, and their use in isoelectric focusing
CN102472724B (zh) * 2009-08-18 2014-08-13 工业研究与发展基金会有限公司 分离分子分析物的方法和装置
EP2467709B1 (en) * 2009-08-18 2018-02-28 Technion Research & Development Foundation Ltd. Proton concentration topographies, methods and devices for producing the same
EP2773959B1 (en) * 2011-11-04 2019-04-10 Bio-Rad Laboratories, Inc. Protein fractionation based on isoelectric focusing

Also Published As

Publication number Publication date
CN103134843B (zh) 2014-11-12
US10132776B2 (en) 2018-11-20
JP2010538255A (ja) 2010-12-09
CN101868425A (zh) 2010-10-20
CA2697649A1 (en) 2009-03-05
EP2559666A2 (en) 2013-02-20
EP2559666B1 (en) 2014-07-23
US9274082B2 (en) 2016-03-01
US20100307920A1 (en) 2010-12-09
EP2193100A2 (en) 2010-06-09
CN101868425B (zh) 2013-01-02
AU2008293381B2 (en) 2012-07-19
AU2008293381A1 (en) 2009-03-05
WO2009027970A3 (en) 2009-05-14
EP2559666A3 (en) 2013-04-03
US20160178578A1 (en) 2016-06-23
WO2009027970A2 (en) 2009-03-05
JP5702142B2 (ja) 2015-04-15
CN103134843A (zh) 2013-06-05
KR20100053672A (ko) 2010-05-20
EP2193100B1 (en) 2014-05-07

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