CA2680123A1 - Polypeptide de fusion permettant de detecter des epitopes combinatoires ou composites conserves dans des proteines non conservees - Google Patents
Polypeptide de fusion permettant de detecter des epitopes combinatoires ou composites conserves dans des proteines non conservees Download PDFInfo
- Publication number
- CA2680123A1 CA2680123A1 CA002680123A CA2680123A CA2680123A1 CA 2680123 A1 CA2680123 A1 CA 2680123A1 CA 002680123 A CA002680123 A CA 002680123A CA 2680123 A CA2680123 A CA 2680123A CA 2680123 A1 CA2680123 A1 CA 2680123A1
- Authority
- CA
- Canada
- Prior art keywords
- antigen
- fusion polypeptide
- polypeptide
- epitopes
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims description 104
- 102000004196 processed proteins & peptides Human genes 0.000 title claims description 86
- 229920001184 polypeptide Polymers 0.000 title claims description 72
- 230000004927 fusion Effects 0.000 title claims description 38
- 238000001514 detection method Methods 0.000 title claims description 19
- 102000004169 proteins and genes Human genes 0.000 title description 35
- 108090000623 proteins and genes Proteins 0.000 title description 35
- 239000002131 composite material Substances 0.000 title description 3
- 239000000427 antigen Substances 0.000 claims description 75
- 102000036639 antigens Human genes 0.000 claims description 75
- 108091007433 antigens Proteins 0.000 claims description 75
- 230000027455 binding Effects 0.000 claims description 48
- 238000000034 method Methods 0.000 claims description 23
- 239000002245 particle Substances 0.000 claims description 20
- 125000000539 amino acid group Chemical group 0.000 claims description 16
- 150000001413 amino acids Chemical class 0.000 claims description 16
- 239000000523 sample Substances 0.000 claims description 13
- 108091008324 binding proteins Proteins 0.000 claims description 11
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 7
- 102000014914 Carrier Proteins Human genes 0.000 claims description 6
- 239000012634 fragment Substances 0.000 claims description 5
- 230000002163 immunogen Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000002703 mutagenesis Methods 0.000 claims description 4
- 231100000350 mutagenesis Toxicity 0.000 claims description 4
- 238000002823 phage display Methods 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 2
- YTAHJIFKAKIKAV-XNMGPUDCSA-N [(1R)-3-morpholin-4-yl-1-phenylpropyl] N-[(3S)-2-oxo-5-phenyl-1,3-dihydro-1,4-benzodiazepin-3-yl]carbamate Chemical compound O=C1[C@H](N=C(C2=C(N1)C=CC=C2)C1=CC=CC=C1)NC(O[C@H](CCN1CCOCC1)C1=CC=CC=C1)=O YTAHJIFKAKIKAV-XNMGPUDCSA-N 0.000 claims description 2
- 238000010205 computational analysis Methods 0.000 claims description 2
- 101710205625 Capsid protein p24 Proteins 0.000 description 18
- 101710177166 Phosphoprotein Proteins 0.000 description 18
- 101710149279 Small delta antigen Proteins 0.000 description 18
- 241000725303 Human immunodeficiency virus Species 0.000 description 16
- 239000003446 ligand Substances 0.000 description 8
- 241000700605 Viruses Species 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 102000023732 binding proteins Human genes 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 238000013459 approach Methods 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 5
- 238000003018 immunoassay Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000028993 immune response Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 3
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 3
- 238000003314 affinity selection Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 108010032595 Antibody Binding Sites Proteins 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 238000012875 competitive assay Methods 0.000 description 2
- 230000001010 compromised effect Effects 0.000 description 2
- 230000009146 cooperative binding Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 2
- 230000036963 noncompetitive effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108090000565 Capsid Proteins Proteins 0.000 description 1
- 102100023321 Ceruloplasmin Human genes 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108010027044 HIV Core Protein p24 Proteins 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000021309 simple sugar Nutrition 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
- C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
- C07K16/1054—Lentiviridae, e.g. HIV, FIV, SIV gag-pol, e.g. p17, p24
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
- G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
- G01N2333/16—HIV-1, HIV-2
- G01N2333/161—HIV-1, HIV-2 gag-pol, e.g. p55, p24/25, p17/18, p.7, p6, p66/68, p51/52, p31/34, p32, p40
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Urology & Nephrology (AREA)
- AIDS & HIV (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US89342007P | 2007-03-07 | 2007-03-07 | |
| FI20075159 | 2007-03-07 | ||
| FI20075159A FI120349B (fi) | 2007-03-07 | 2007-03-07 | Menetelmä fuusiopolypeptidin valmistamiseksi |
| US60/893,420 | 2007-03-07 | ||
| PCT/FI2008/050111 WO2008107523A2 (fr) | 2007-03-07 | 2008-03-07 | Polypeptide de fusion permettant de détecter des épitopes combinatoires ou composites conservés dans des protéines non conservées |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2680123A1 true CA2680123A1 (fr) | 2008-09-12 |
Family
ID=37930071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002680123A Abandoned CA2680123A1 (fr) | 2007-03-07 | 2008-03-07 | Polypeptide de fusion permettant de detecter des epitopes combinatoires ou composites conserves dans des proteines non conservees |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8735328B2 (fr) |
| EP (1) | EP2129689A4 (fr) |
| JP (1) | JP2010520266A (fr) |
| CN (1) | CN101679514A (fr) |
| AU (1) | AU2008223755A1 (fr) |
| CA (1) | CA2680123A1 (fr) |
| FI (1) | FI120349B (fr) |
| MX (1) | MX2009009503A (fr) |
| WO (1) | WO2008107523A2 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7176724B2 (ja) | 2018-07-20 | 2022-11-22 | 国立大学法人滋賀医科大学 | ヒト末梢性コリン作動性神経検出用抗体 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO314588B1 (no) | 2000-09-04 | 2003-04-14 | Bionor Immuno As | HIV-peptider, antigener, vaksinesammensetning, immunoassay- testsett og en fremgangsmåte for å påvise antistoffer indusert av HIV |
| US6610472B1 (en) | 2000-10-31 | 2003-08-26 | Genetastix Corporation | Assembly and screening of highly complex and fully human antibody repertoire in yeast |
| US6833441B2 (en) | 2001-08-01 | 2004-12-21 | Abmaxis, Inc. | Compositions and methods for generating chimeric heteromultimers |
| GB0305702D0 (en) * | 2003-03-12 | 2003-04-16 | Univ Birmingham | Bispecific antibodies |
-
2007
- 2007-03-07 FI FI20075159A patent/FI120349B/fi not_active IP Right Cessation
-
2008
- 2008-03-07 US US12/530,019 patent/US8735328B2/en not_active Expired - Fee Related
- 2008-03-07 JP JP2009552239A patent/JP2010520266A/ja not_active Withdrawn
- 2008-03-07 WO PCT/FI2008/050111 patent/WO2008107523A2/fr active Application Filing
- 2008-03-07 CN CN200880015324A patent/CN101679514A/zh active Pending
- 2008-03-07 AU AU2008223755A patent/AU2008223755A1/en not_active Abandoned
- 2008-03-07 MX MX2009009503A patent/MX2009009503A/es not_active Application Discontinuation
- 2008-03-07 EP EP08718564A patent/EP2129689A4/fr not_active Withdrawn
- 2008-03-07 CA CA002680123A patent/CA2680123A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8735328B2 (en) | 2014-05-27 |
| WO2008107523A8 (fr) | 2009-07-30 |
| FI20075159A (fi) | 2008-09-08 |
| US20100190655A1 (en) | 2010-07-29 |
| WO2008107523A2 (fr) | 2008-09-12 |
| AU2008223755A1 (en) | 2008-09-12 |
| FI20075159A0 (fi) | 2007-03-07 |
| EP2129689A4 (fr) | 2011-11-02 |
| CN101679514A (zh) | 2010-03-24 |
| MX2009009503A (es) | 2010-02-17 |
| JP2010520266A (ja) | 2010-06-10 |
| EP2129689A1 (fr) | 2009-12-09 |
| FI120349B (fi) | 2009-09-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20130225 |
|
| FZDE | Discontinued |
Effective date: 20151109 |
|
| FZDE | Discontinued |
Effective date: 20151109 |