CA2672246A1 - Detecting nucleic acids - Google Patents
Detecting nucleic acids Download PDFInfo
- Publication number
- CA2672246A1 CA2672246A1 CA002672246A CA2672246A CA2672246A1 CA 2672246 A1 CA2672246 A1 CA 2672246A1 CA 002672246 A CA002672246 A CA 002672246A CA 2672246 A CA2672246 A CA 2672246A CA 2672246 A1 CA2672246 A1 CA 2672246A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- target nucleic
- probe
- target
- mirna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C12Q1/6832—Enhancement of hybridisation reaction
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- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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| US8802599B2 (en) * | 2007-03-27 | 2014-08-12 | Rosetta Genomics, Ltd. | Gene expression signature for classification of tissue of origin of tumor samples |
| US20100273172A1 (en) | 2007-03-27 | 2010-10-28 | Rosetta Genomics Ltd. | Micrornas expression signature for determination of tumors origin |
| US9096906B2 (en) * | 2007-03-27 | 2015-08-04 | Rosetta Genomics Ltd. | Gene expression signature for classification of tissue of origin of tumor samples |
| JP2010535782A (ja) | 2007-07-31 | 2010-11-25 | ズィ、オハイオウ、ステイト、ユーニヴァーサティ、リサーチ、ファウンデイシャン | Ｄｎｍｔ３ａ及びｄｎｍｔ３ｂを標的にすることによるメチル化を元に戻す方法 |
| ES2570359T3 (es) | 2007-08-03 | 2016-05-18 | Univ Ohio State Res Found | Regiones ultraconservadas que codifican ARNnc |
| JP5770472B2 (ja) | 2007-08-22 | 2015-08-26 | ジ・オハイオ・ステイト・ユニバーシティ・リサーチ・ファウンデイションＴｈｅ Ｏｈｉｏ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ Ｒｅｓｅａｒｃｈ Ｆｏｕｎｄａｔｉｏｎ | ヒト急性白血病におけるｅｐｈａ７及びｅｒｋリン酸化の調節解除を誘発するための方法及び組成物 |
| US8936909B2 (en) | 2008-07-18 | 2015-01-20 | Qiagen Gmbh | Method for determining the origin of a sample |
| US20100068711A1 (en) | 2008-07-18 | 2010-03-18 | Xenomics, Inc. | Methods of PCR-Based Detection of "Ultra Short" Nucleic Acid Sequences |
| WO2010018564A1 (en) * | 2008-08-13 | 2010-02-18 | Rosetta Genomics Ltd. | Compositions and methods for determining the prognosis of bladder urothelial cancer |
| WO2010058393A2 (en) * | 2008-11-20 | 2010-05-27 | Rosetta Genomics Ltd. | Compositions and methods for the prognosis of colon cancer |
| AU2009322907B2 (en) * | 2008-12-05 | 2016-05-05 | The Ohio State University Research Foundation | MicroRNA-based methods and compositions for the diagnosis and treatment of ovarian cancer |
| WO2010103522A1 (en) * | 2009-03-10 | 2010-09-16 | Rosetta Genomics Ltd. | Method for detection of nucleic acid sequences |
| US20120165208A1 (en) * | 2009-05-18 | 2012-06-28 | Inserm (Institut National De La Sante Et De La Recherche Medicale) | method for predicting the responsiveness of a patient to a treatment with an anti-cd20 antibody and a method for diagnosing rheumatoid arthritis |
| CN102803511A (zh) | 2009-11-23 | 2012-11-28 | 俄亥俄州立大学 | 用于影响肿瘤细胞生长、迁移和侵袭的材料和方法 |
| US9476101B2 (en) * | 2010-06-07 | 2016-10-25 | Firefly Bioworks, Inc. | Scanning multifunctional particles |
| AU2011326032B2 (en) | 2010-11-12 | 2016-10-06 | The Ohio State University Research Foundation | Materials and methods related to microRNA-21, mismatch repair, and colorectal cancer |
| US9163329B2 (en) | 2010-11-12 | 2015-10-20 | Agilent Technologies, Inc. | RNA labeling method |
| WO2012068147A1 (en) | 2010-11-15 | 2012-05-24 | The Ohio State University Research Foundation | Controlled release mucoadhesive systems |
| US8703653B2 (en) | 2011-02-18 | 2014-04-22 | NVS Technologies, Inc. | Quantitative, highly multiplexed detection of nucleic acids |
| ES2703133T3 (es) * | 2011-05-24 | 2019-03-07 | Rosetta Genomics Ltd | Métodos y composiciones para determinar insuficiencia cardiaca o un riesgo de insuficiencia cardiaca |
| US9249468B2 (en) | 2011-10-14 | 2016-02-02 | The Ohio State University | Methods and materials related to ovarian cancer |
| CA2855953A1 (en) * | 2011-11-17 | 2013-05-23 | NVS Technologies, Inc. | Quantitative, highly multiplexed detection of nucleic acids |
| AU2012352265B2 (en) | 2011-12-13 | 2017-02-16 | Ohio State Innovation Foundation | Methods and compositions related to miR-21 and miR-29a, exosome inhibition, and cancer metastasis |
| CN105936932A (zh) | 2012-01-20 | 2016-09-14 | 俄亥俄州立大学 | 浸润性和预后的乳腺癌生物标志物标签 |
| WO2013148498A1 (en) | 2012-03-27 | 2013-10-03 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
| JP2015530113A (ja) * | 2012-09-28 | 2015-10-15 | セファイド | マイクロｒｎａ多重アッセイのための２プライマーｐｃｒ |
| EP3748016A1 (en) | 2012-11-02 | 2020-12-09 | Life Technologies Corporation | Novel compositions and methods for enhancing pcr specificity |
| KR101922125B1 (ko) | 2012-11-29 | 2018-11-26 | 삼성전자주식회사 | 표적 핵산을 표지하는 방법 |
| US10758526B2 (en) | 2014-06-09 | 2020-09-01 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services National Institutes Of Health | Combination therapies using agents that target tumor-associated stroma or tumor cells and other pathways |
| WO2015191568A2 (en) | 2014-06-09 | 2015-12-17 | Biomed Valley Discoveries, Inc. | Combination therapies using agents that target tumor-associated stroma or tumor cells and tumor vasculature |
| US11033620B2 (en) | 2014-06-09 | 2021-06-15 | Biomed Valley Discoveries, Inc. | Combination therapies targeting tumor-associated stroma or tumor cells and microtubules |
| WO2015191596A1 (en) | 2014-06-09 | 2015-12-17 | Biomed Valley Discoveries, Inc. | Combination therapies using platinum agents and agents that target tumor-associated stroma or tumor cells |
| WO2015191615A2 (en) | 2014-06-09 | 2015-12-17 | Biomed Valley Discoveries, Inc. | Combination therapies using agents that target tumor-associated stroma or tumor cells and alkylating agents |
| WO2015191583A2 (en) | 2014-06-09 | 2015-12-17 | Biomed Valley Discoveries, Inc. | Combination therapies targeting tumor-associated stroma or tumor cells and topoisomerase |
| WO2015191617A2 (en) | 2014-06-09 | 2015-12-17 | Biomed Valley Discoveries, Inc. | Combination therapies using anti-metabolites and agents that target tumor-associated stroma or tumor cells |
| EP3259373B1 (en) * | 2015-02-17 | 2022-07-27 | Bio-Rad Laboratories, Inc. | Small nucleic acid quantification using split cycle amplification |
| EP3967768A1 (en) | 2015-03-13 | 2022-03-16 | Life Technologies Corporation | Compositions for small rna capture, detection and quantification |
| EP3070178B1 (en) * | 2015-03-20 | 2020-02-12 | Albert-Ludwigs-Universität Freiburg | Method for diagnosing breast cancer |
| CN113005181B (zh) * | 2020-12-22 | 2022-01-18 | 广州血康陆道培生物技术有限公司 | 一种基于茎环法的多重荧光定量pcr检测非编码小rna的引物组 |
| JP7743246B2 (ja) * | 2021-09-29 | 2025-09-24 | 栄研化学株式会社 | 標的ｒｎａ検出方法及びプローブキット |
Family Cites Families (46)
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|---|---|---|---|---|
| US4766064A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay employing polyether and diagnostic kit |
| US5430136A (en) * | 1984-10-16 | 1995-07-04 | Chiron Corporation | Oligonucleotides having selectably cleavable and/or abasic sites |
| US5034506A (en) * | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| ATE88761T1 (de) | 1986-01-10 | 1993-05-15 | Amoco Corp | Kompetitiver homogener test. |
| US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5359100A (en) * | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5597909A (en) * | 1994-08-25 | 1997-01-28 | Chiron Corporation | Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use |
| US5580731A (en) | 1994-08-25 | 1996-12-03 | Chiron Corporation | N-4 modified pyrimidine deoxynucleotides and oligonucleotide probes synthesized therewith |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5780233A (en) * | 1996-06-06 | 1998-07-14 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| US6235480B1 (en) * | 1998-03-13 | 2001-05-22 | Promega Corporation | Detection of nucleic acid hybrids |
| US6270973B1 (en) * | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
| AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
| GB9817078D0 (en) * | 1998-08-05 | 1998-10-07 | Habib Nagy A | Device for liver surgery |
| ATE501269T1 (de) * | 1999-03-18 | 2011-03-15 | Exiqon As | Detektion von genmutationen mittels lna primer |
| EP1130113A1 (en) * | 2000-02-15 | 2001-09-05 | Johannes Petrus Schouten | Multiplex ligation dependent amplification assay |
| EP1130119A1 (en) | 2000-02-17 | 2001-09-05 | Amsterdam Support Diagnostics B.V. | Reducing background in hybridisation reactions |
| US6653081B2 (en) | 2000-06-12 | 2003-11-25 | Virologic, Inc. | Methods for monitoring antiretroviral therapy and guiding therapeutic decision in the treatment of HIV/AIDS |
| US20020102557A1 (en) * | 2001-01-31 | 2002-08-01 | Gentile-Davey Maria C. | Self -complementary molecular probe |
| EP1280090A1 (de) | 2001-07-27 | 2003-01-29 | iSenseIt AG | Verfahren zur Konfiguration paralleler Nukleinsäureanalyseverfahren |
| JP4554206B2 (ja) * | 2001-09-27 | 2010-09-29 | ガンガゲン インコーポレーティッド | 無力化した全菌体免疫原性細菌組成物 |
| KR20030042581A (ko) | 2001-11-23 | 2003-06-02 | 삼성전자주식회사 | 대립유전자－특이적 프라이머 연장반응에서 프라이머간의 식별을 증가시키는 방법 |
| WO2003078450A2 (en) | 2002-03-11 | 2003-09-25 | Epoch Biosciences, Inc. | Negatively charged minor groove binders |
| JP2004024247A (ja) * | 2002-04-30 | 2004-01-29 | Internatl Reagents Corp | 遺伝子変異検出用プローブ及び遺伝子変異検出方法 |
| US6797464B2 (en) * | 2002-07-10 | 2004-09-28 | University Of Massachusetts | Detection of drug-resistant human immunodeficiency virus |
| WO2004094595A2 (en) * | 2003-04-17 | 2004-11-04 | Alnylam Pharmaceuticals Inc. | MODIFIED iRNA AGENTS |
| US7745117B2 (en) * | 2003-06-06 | 2010-06-29 | The Regents Of The University Of Michigan | Methods for incorporating non-perfectly matched oligonucleotides into target-specific hybridization sequences |
| US7320857B2 (en) | 2003-06-18 | 2008-01-22 | Chinese National Human Genome Center At Shanghai | Characterization of the earliest stages of the severe acute respiratory syndrome (SARS) virus and uses thereof |
| JP2007525963A (ja) * | 2003-06-20 | 2007-09-13 | イルミナ インコーポレイテッド | 全ゲノム増幅および遺伝型決定のための方法および組成物 |
| KR100936908B1 (ko) | 2003-07-18 | 2010-01-18 | 삼성전자주식회사 | 전계발광 디바이스의 박막 트랜지스터, 이를 이용한전계발광 디바이스 및 이의 제조 방법 |
| US7888497B2 (en) * | 2003-08-13 | 2011-02-15 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof |
| WO2005033340A2 (en) * | 2003-09-30 | 2005-04-14 | Applera Corporation | Methods and kits for hybridizing multiple probe panels to nucleic acid samples |
| US20050182005A1 (en) * | 2004-02-13 | 2005-08-18 | Tuschl Thomas H. | Anti-microRNA oligonucleotide molecules |
| US7127355B2 (en) | 2004-03-05 | 2006-10-24 | Perlegen Sciences, Inc. | Methods for genetic analysis |
| US8192937B2 (en) * | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
| WO2005116250A2 (en) * | 2004-05-26 | 2005-12-08 | Rosetta Genomics Ltd. | Viral and viral associated mirnas and uses thereof |
| US7825229B2 (en) * | 2005-03-25 | 2010-11-02 | Rosetta Genomics Ltd. | Lung cancer-related nucleic acids |
| US7642348B2 (en) * | 2004-10-04 | 2010-01-05 | Rosetta Genomics Ltd | Prostate cancer-related nucleic acids |
| KR100682923B1 (ko) | 2005-01-22 | 2007-02-15 | 삼성전자주식회사 | 허들 ｄｎａ와 인공적인 미스매치가 도입된 프로브를이용한 단일 염기 미스매치에 대한 구별 향상 방법 |
| WO2006126040A1 (en) | 2005-05-25 | 2006-11-30 | Rosetta Genomics Ltd. | Bacterial and bacterial associated mirnas and uses thereof |
| CN100510105C (zh) | 2006-02-08 | 2009-07-08 | 博奥生物有限公司 | 一种序列特异性寡核苷酸探针及其应用 |
| KR100813263B1 (ko) | 2006-08-17 | 2008-03-13 | 삼성전자주식회사 | 표적 서열 검출용 프로브 설계 방법 및 상기 프로브를이용한 표적 서열 검출 방법 |
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2007
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- 2007-06-29 JP JP2009517484A patent/JP2010500867A/ja active Pending
- 2007-06-29 DK DK07848974.7T patent/DK2038432T3/en active
- 2007-06-29 US US12/308,954 patent/US9115389B2/en not_active Expired - Fee Related
- 2007-06-29 WO PCT/IB2007/003732 patent/WO2008029295A2/en not_active Ceased
- 2007-06-29 AU AU2007293187A patent/AU2007293187A1/en not_active Abandoned
- 2007-06-29 CA CA002672246A patent/CA2672246A1/en not_active Abandoned
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2008
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2015
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| US20150376695A1 (en) | 2015-12-31 |
| IL196241A0 (en) | 2009-09-22 |
| US20100047784A1 (en) | 2010-02-25 |
| US9115389B2 (en) | 2015-08-25 |
| EP2038432B1 (en) | 2017-02-08 |
| WO2008029295A2 (en) | 2008-03-13 |
| EP2038432A2 (en) | 2009-03-25 |
| AU2007293187A1 (en) | 2008-03-13 |
| WO2008029295A3 (en) | 2011-05-12 |
| EP2038432A4 (en) | 2012-05-30 |
| JP2010500867A (ja) | 2010-01-14 |
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