CA2672246A1 - Detecting nucleic acids - Google Patents

Detecting nucleic acids Download PDF

Info

Publication number
CA2672246A1
CA2672246A1 CA002672246A CA2672246A CA2672246A1 CA 2672246 A1 CA2672246 A1 CA 2672246A1 CA 002672246 A CA002672246 A CA 002672246A CA 2672246 A CA2672246 A CA 2672246A CA 2672246 A1 CA2672246 A1 CA 2672246A1
Authority
CA
Canada
Prior art keywords
nucleic acid
target nucleic
probe
target
mirna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002672246A
Other languages
English (en)
French (fr)
Inventor
Gilad Shlomit
Esther Meiri
Noga Yerushalmi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rosetta Genomics Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2672246A1 publication Critical patent/CA2672246A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/136Screening for pharmacological compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002672246A 2006-06-30 2007-06-29 Detecting nucleic acids Abandoned CA2672246A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US80640406P 2006-06-30 2006-06-30
US60/806,404 2006-06-30
US82237106P 2006-08-14 2006-08-14
US60/822,371 2006-08-14
US85045506P 2006-10-10 2006-10-10
US60/850,455 2006-10-10
US87109506P 2006-12-20 2006-12-20
US60/871,095 2006-12-20
PCT/IB2007/003732 WO2008029295A2 (en) 2006-06-30 2007-06-29 A method for detecting nucleic acids

Publications (1)

Publication Number Publication Date
CA2672246A1 true CA2672246A1 (en) 2008-03-13

Family

ID=39157641

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002672246A Abandoned CA2672246A1 (en) 2006-06-30 2007-06-29 Detecting nucleic acids

Country Status (8)

Country Link
US (2) US9115389B2 (enExample)
EP (1) EP2038432B1 (enExample)
JP (1) JP2010500867A (enExample)
AU (1) AU2007293187A1 (enExample)
CA (1) CA2672246A1 (enExample)
DK (1) DK2038432T3 (enExample)
IL (1) IL196241A0 (enExample)
WO (1) WO2008029295A2 (enExample)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2487255A3 (en) 2006-01-05 2012-10-10 The Ohio State University Research Foundation MicroRNA-based methods and compositions for the diagnosis and treatment of solid cancers
EP2371971B1 (en) 2006-03-20 2013-11-27 The Ohio State University Research Foundation Microrna fingerprints during human megakaryocytopoiesis
US8802599B2 (en) * 2007-03-27 2014-08-12 Rosetta Genomics, Ltd. Gene expression signature for classification of tissue of origin of tumor samples
US20100273172A1 (en) 2007-03-27 2010-10-28 Rosetta Genomics Ltd. Micrornas expression signature for determination of tumors origin
US9096906B2 (en) * 2007-03-27 2015-08-04 Rosetta Genomics Ltd. Gene expression signature for classification of tissue of origin of tumor samples
US8367632B2 (en) 2007-07-31 2013-02-05 Ohio State University Research Foundation Methods for reverting methylation by targeting methyltransferases
US8465918B2 (en) 2007-08-03 2013-06-18 The Ohio State University Research Foundation Ultraconserved regions encoding ncRNAs
WO2009026487A1 (en) 2007-08-22 2009-02-26 The Ohio State University Research Foundation Methods and compositions for inducing deregulation of epha7 and erk phosphorylation in human acute leukemias
WO2010006814A1 (en) * 2008-07-18 2010-01-21 Qiagen Gmbh Method for determining the origin of a sample
US20100068711A1 (en) 2008-07-18 2010-03-18 Xenomics, Inc. Methods of PCR-Based Detection of "Ultra Short" Nucleic Acid Sequences
WO2010018564A1 (en) * 2008-08-13 2010-02-18 Rosetta Genomics Ltd. Compositions and methods for determining the prognosis of bladder urothelial cancer
WO2010058393A2 (en) * 2008-11-20 2010-05-27 Rosetta Genomics Ltd. Compositions and methods for the prognosis of colon cancer
US20110275534A1 (en) * 2008-12-05 2011-11-10 The Ohio State University MicroRNA-Based Methods and Compositions for the Diagnosis, Prognosis and Treatment of Ovarian Cancer Using a Real-Time PCR Platform
WO2010103522A1 (en) * 2009-03-10 2010-09-16 Rosetta Genomics Ltd. Method for detection of nucleic acid sequences
US20120165208A1 (en) * 2009-05-18 2012-06-28 Inserm (Institut National De La Sante Et De La Recherche Medicale) method for predicting the responsiveness of a patient to a treatment with an anti-cd20 antibody and a method for diagnosing rheumatoid arthritis
CA2781547A1 (en) 2009-11-23 2011-05-26 The Ohio State University Materials and methods useful for affecting tumor cell growth, migration and invasion
US9476101B2 (en) * 2010-06-07 2016-10-25 Firefly Bioworks, Inc. Scanning multifunctional particles
US9163329B2 (en) 2010-11-12 2015-10-20 Agilent Technologies, Inc. RNA labeling method
AU2011326032B2 (en) 2010-11-12 2016-10-06 The Ohio State University Research Foundation Materials and methods related to microRNA-21, mismatch repair, and colorectal cancer
CN103313706A (zh) 2010-11-15 2013-09-18 俄亥俄州立大学研究基金会 控制释放粘膜粘合系统
US8703653B2 (en) 2011-02-18 2014-04-22 NVS Technologies, Inc. Quantitative, highly multiplexed detection of nucleic acids
ES2703133T3 (es) * 2011-05-24 2019-03-07 Rosetta Genomics Ltd Métodos y composiciones para determinar insuficiencia cardiaca o un riesgo de insuficiencia cardiaca
WO2013056217A1 (en) 2011-10-14 2013-04-18 The Ohio State University Methods and materials related to ovarian cancer
KR20140094007A (ko) * 2011-11-17 2014-07-29 엔브이에스 테크놀로지스 인코포레이티드 핵산의 고도로 다중화된 정량 검출
US9481885B2 (en) 2011-12-13 2016-11-01 Ohio State Innovation Foundation Methods and compositions related to miR-21 and miR-29a, exosome inhibition, and cancer metastasis
EP2804960A4 (en) 2012-01-20 2015-08-19 Univ Ohio State BREAST CANCER BIOMARK SIGNATURES FOR INVASIVITY AND FORECAST
US10041950B2 (en) 2012-03-27 2018-08-07 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
WO2014052487A1 (en) 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
EP2914741B1 (en) 2012-11-02 2017-08-16 Life Technologies Corporation Novel compositions and methods for enhancing pcr specificity
KR101922125B1 (ko) 2012-11-29 2018-11-26 삼성전자주식회사 표적 핵산을 표지하는 방법
WO2015191615A2 (en) 2014-06-09 2015-12-17 Biomed Valley Discoveries, Inc. Combination therapies using agents that target tumor-associated stroma or tumor cells and alkylating agents
WO2015191610A2 (en) 2014-06-09 2015-12-17 Biomed Valley Discoveries, Inc. Combination therapies using agents that target tumor-associated stroma or tumor cells and other pathways
WO2015191617A2 (en) 2014-06-09 2015-12-17 Biomed Valley Discoveries, Inc. Combination therapies using anti-metabolites and agents that target tumor-associated stroma or tumor cells
US11034757B2 (en) 2014-06-09 2021-06-15 Biomed Valley Discoveries, Inc. Combination therapies using agents that target tumor-associated stroma or tumor cells and tumor vasculature
WO2015191596A1 (en) 2014-06-09 2015-12-17 Biomed Valley Discoveries, Inc. Combination therapies using platinum agents and agents that target tumor-associated stroma or tumor cells
US10758614B2 (en) 2014-06-09 2020-09-01 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services National Institutes Of Health Combination therapies targeting tumor-associated stroma or tumor cells and topoisomerase
US11033620B2 (en) 2014-06-09 2021-06-15 Biomed Valley Discoveries, Inc. Combination therapies targeting tumor-associated stroma or tumor cells and microtubules
EP3486329A1 (en) * 2015-02-17 2019-05-22 Bio-Rad Laboratories, Inc. Method of generating and amplifying cdna
EP3268491B1 (en) 2015-03-13 2021-07-14 Life Technologies Corporation Methods, compositions and kits for small rna capture, detection and quantification
EP3070178B1 (en) * 2015-03-20 2020-02-12 Albert-Ludwigs-Universität Freiburg Method for diagnosing breast cancer
CN113005181B (zh) * 2020-12-22 2022-01-18 广州血康陆道培生物技术有限公司 一种基于茎环法的多重荧光定量pcr检测非编码小rna的引物组
JP7743246B2 (ja) * 2021-09-29 2025-09-24 栄研化学株式会社 標的rna検出方法及びプローブキット

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766064A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US5430136A (en) 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5359100A (en) 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5580731A (en) 1994-08-25 1996-12-03 Chiron Corporation N-4 modified pyrimidine deoxynucleotides and oligonucleotide probes synthesized therewith
US5597909A (en) 1994-08-25 1997-01-28 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5780233A (en) * 1996-06-06 1998-07-14 Wisconsin Alumni Research Foundation Artificial mismatch hybridization
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
GB9817078D0 (en) 1998-08-05 1998-10-07 Habib Nagy A Device for liver surgery
IL145417A0 (en) 1999-03-18 2002-06-30 Exiqon As Detection of mutations in genes by specific lna primers
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
EP1130119A1 (en) * 2000-02-17 2001-09-05 Amsterdam Support Diagnostics B.V. Reducing background in hybridisation reactions
US6653081B2 (en) 2000-06-12 2003-11-25 Virologic, Inc. Methods for monitoring antiretroviral therapy and guiding therapeutic decision in the treatment of HIV/AIDS
US20020102557A1 (en) * 2001-01-31 2002-08-01 Gentile-Davey Maria C. Self -complementary molecular probe
EP1280090A1 (de) * 2001-07-27 2003-01-29 iSenseIt AG Verfahren zur Konfiguration paralleler Nukleinsäureanalyseverfahren
US6913753B2 (en) 2001-09-27 2005-07-05 Gangagen, Inc. Incapacitated whole-cell immunogenic bacterial compositions
KR20030042581A (ko) * 2001-11-23 2003-06-02 삼성전자주식회사 대립유전자-특이적 프라이머 연장반응에서 프라이머간의 식별을 증가시키는 방법
US7582739B2 (en) 2002-03-11 2009-09-01 Epoch Biosciences, Inc. Negatively charged minor groove binders
JP2004024247A (ja) * 2002-04-30 2004-01-29 Internatl Reagents Corp 遺伝子変異検出用プローブ及び遺伝子変異検出方法
US6797464B2 (en) 2002-07-10 2004-09-28 University Of Massachusetts Detection of drug-resistant human immunodeficiency virus
ES2702942T3 (es) 2003-04-17 2019-03-06 Alnylam Pharmaceuticals Inc Agentes de ARNi modificados
WO2004108950A2 (en) * 2003-06-06 2004-12-16 The Regents Of The University Of Michigan Methods for incorporating non-perfectly matched oligonucleotides into target-specific hybridization sequences
EP1649053A4 (en) 2003-06-18 2006-10-18 Chinese Nat Human Genome Ct Sh CHARACTERIZATION OF THE EARLY LEVELS OF THE VIRUS OF HEAVY ACUTE RESPIRATORY SYNDROME (SARS) AND USES THEREOF
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
KR100936908B1 (ko) 2003-07-18 2010-01-18 삼성전자주식회사 전계발광 디바이스의 박막 트랜지스터, 이를 이용한전계발광 디바이스 및 이의 제조 방법
US7888497B2 (en) 2003-08-13 2011-02-15 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof
US20050123959A1 (en) * 2003-09-30 2005-06-09 Brett Williams Methods and kits for hybridizing multiple probe panels to nucleic acid samples
US20050182005A1 (en) 2004-02-13 2005-08-18 Tuschl Thomas H. Anti-microRNA oligonucleotide molecules
US7127355B2 (en) 2004-03-05 2006-10-24 Perlegen Sciences, Inc. Methods for genetic analysis
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
CN101031658A (zh) 2004-05-26 2007-09-05 罗塞塔金诺米克斯有限公司 病毒和病毒相关mirna及其用途
US7825229B2 (en) 2005-03-25 2010-11-02 Rosetta Genomics Ltd. Lung cancer-related nucleic acids
US7642348B2 (en) 2004-10-04 2010-01-05 Rosetta Genomics Ltd Prostate cancer-related nucleic acids
KR100682923B1 (ko) * 2005-01-22 2007-02-15 삼성전자주식회사 허들 dna와 인공적인 미스매치가 도입된 프로브를이용한 단일 염기 미스매치에 대한 구별 향상 방법
WO2006126040A1 (en) 2005-05-25 2006-11-30 Rosetta Genomics Ltd. Bacterial and bacterial associated mirnas and uses thereof
CN100510105C (zh) * 2006-02-08 2009-07-08 博奥生物有限公司 一种序列特异性寡核苷酸探针及其应用
KR100813263B1 (ko) * 2006-08-17 2008-03-13 삼성전자주식회사 표적 서열 검출용 프로브 설계 방법 및 상기 프로브를이용한 표적 서열 검출 방법

Also Published As

Publication number Publication date
EP2038432B1 (en) 2017-02-08
JP2010500867A (ja) 2010-01-14
DK2038432T3 (en) 2017-04-03
WO2008029295A2 (en) 2008-03-13
US20100047784A1 (en) 2010-02-25
WO2008029295A3 (en) 2011-05-12
US20150376695A1 (en) 2015-12-31
IL196241A0 (en) 2009-09-22
EP2038432A2 (en) 2009-03-25
US9115389B2 (en) 2015-08-25
AU2007293187A1 (en) 2008-03-13
EP2038432A4 (en) 2012-05-30

Similar Documents

Publication Publication Date Title
US9115389B2 (en) Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid
Jonstrup et al. A microRNA detection system based on padlock probes and rolling circle amplification
Ren et al. A highly sensitive and selective electrochemical biosensor for direct detection of microRNAs in serum
Chen et al. Highly sensitive and specific microRNA expression profiling using BeadArray technology
US8399222B2 (en) Compositions and methods for detecting small RNAs, and uses thereof
Li et al. Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis
EP2419538B1 (en) Methods and compositions to detect and differentiate small rnas in rna maturation pathway
Bak et al. MicroRNA expression in the adult mouse central nervous system
Guerau-de-Arellano et al. miRNA profiling for biomarker discovery in multiple sclerosis: from microarray to deep sequencing
US7642348B2 (en) Prostate cancer-related nucleic acids
US7825229B2 (en) Lung cancer-related nucleic acids
US7993831B2 (en) Methods of normalization in microRNA detection assays
US20080241831A1 (en) Methods for detecting small RNA species
US20080076674A1 (en) Novel oligonucleotide compositions and probe sequences useful for detection and analysis of non coding RNAs associated with cancer
US20070065844A1 (en) Solution-based methods for RNA expression profiling
US9677130B2 (en) Methods to detect and quantify RNA
US20160215328A1 (en) Methods, compositions and systems for the analysis of nucleic acid molecules
Ab Mutalib et al. Molecular profiling and detection methods of microRNA in cancer research
US20150299788A1 (en) Rna detection method and detection kit
Zhang et al. In situ hybridization-based detection of microRNAs in human diseases
WO2010058393A2 (en) Compositions and methods for the prognosis of colon cancer
Einat Methodologies for high-throughput expression profiling of microRNAs
Zhang et al. Conventional miRNA detection strategies
Mattie et al. Breast and Prostate Biopsies: Use of Optimized High-Throughput MicroRNA Expression for Diagnosis (Methodology)
Castoldi et al. 12 How to Assay

Legal Events

Date Code Title Description
FZDE Discontinued