CA2663052A1 - Genetically programmed expression of selectively sulfated proteins in eubacteria - Google Patents

Genetically programmed expression of selectively sulfated proteins in eubacteria Download PDF

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Publication number
CA2663052A1
CA2663052A1 CA002663052A CA2663052A CA2663052A1 CA 2663052 A1 CA2663052 A1 CA 2663052A1 CA 002663052 A CA002663052 A CA 002663052A CA 2663052 A CA2663052 A CA 2663052A CA 2663052 A1 CA2663052 A1 CA 2663052A1
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CA
Canada
Prior art keywords
trna
amino acid
unnatural amino
translation system
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002663052A
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English (en)
French (fr)
Inventor
Chang C. Liu
Peter G. Schultz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2663052A1 publication Critical patent/CA2663052A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/815Protease inhibitors from leeches, e.g. hirudin, eglin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002663052A 2006-09-21 2007-09-20 Genetically programmed expression of selectively sulfated proteins in eubacteria Abandoned CA2663052A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US84651906P 2006-09-21 2006-09-21
US60/846,519 2006-09-21
US85521006P 2006-10-28 2006-10-28
US60/855,210 2006-10-28
PCT/US2007/020459 WO2008036392A2 (en) 2006-09-21 2007-09-20 Genetically programmed expression of selectively sulfated proteins in eubacteria

Publications (1)

Publication Number Publication Date
CA2663052A1 true CA2663052A1 (en) 2008-03-27

Family

ID=39201110

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002663052A Abandoned CA2663052A1 (en) 2006-09-21 2007-09-20 Genetically programmed expression of selectively sulfated proteins in eubacteria

Country Status (15)

Country Link
US (3) US8114652B2 (enExample)
EP (1) EP2052083B1 (enExample)
JP (1) JP5385143B2 (enExample)
KR (1) KR20090074765A (enExample)
CN (1) CN101516901B (enExample)
AU (1) AU2007297610A1 (enExample)
BR (1) BRPI0716963A2 (enExample)
CA (1) CA2663052A1 (enExample)
ES (1) ES2456331T3 (enExample)
IL (1) IL197531A0 (enExample)
MX (1) MX2009002987A (enExample)
PL (1) PL2052083T3 (enExample)
PT (1) PT2052083E (enExample)
RU (1) RU2009110152A (enExample)
WO (1) WO2008036392A2 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) * 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
BRPI0819092A2 (pt) * 2007-11-02 2014-10-14 Scripps Research Inst Evolução direcionada usando proteínas que compreendem aminoácidos não naturais.
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN102888387B (zh) * 2011-07-21 2015-03-18 中国科学院生物物理研究所 3-氯代酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
WO2015120287A2 (en) 2014-02-06 2015-08-13 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2016073079A2 (en) 2014-09-26 2016-05-12 Yale University Compositions and methods for biocontainment of microorganisms
CN110117580B (zh) * 2018-02-05 2021-12-14 中国科学院生物物理研究所 硒代酪氨酸翻译系统及其应用
IL280198B1 (en) * 2018-07-18 2025-09-01 Regenxbio Inc Treatment of mucopolysaccharidosis I with fully glycosylated human iduronidase-alpha-L (idus)
US12371817B2 (en) * 2019-03-04 2025-07-29 The Texas A&M University System Methods of making and utilizing amber-obligated phage display libraries
US11149280B2 (en) 2019-10-29 2021-10-19 Yale University Engineering organisms resistant to viruses and horizontally transferred genetic elements
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
CN113789335B (zh) * 2021-11-16 2022-03-15 江苏省中国科学院植物研究所 一种水蛭酪氨酸磺酸转移酶基因及其应用
CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204016B1 (en) 1998-01-29 2001-03-20 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
EP1490483B1 (en) 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
DK1578949T3 (da) * 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
KR20050073559A (ko) 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
EP1583816A4 (en) 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
WO2005003294A2 (en) 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
WO2005038002A2 (en) 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
BRPI0417801A (pt) 2003-12-18 2007-04-10 Scripps Research Inst incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas
AU2005248392A1 (en) 2004-05-25 2005-12-08 Irm, Llc Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
EP1797177A4 (en) 2004-09-21 2008-07-09 Scripps Research Inst ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE
EP1794323B1 (en) 2004-09-21 2014-08-06 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
JP2008513806A (ja) 2004-09-22 2008-05-01 ザ スクリップス リサーチ インスティテュート Nmr試験のための蛋白質の部位特異的標識
EP1809758A4 (en) 2004-10-20 2009-04-01 Scripps Research Inst LOCAL IN VIVO INSTALLATION OF N-ACETYLGALACTOSAMINE AMINO ACIDS IN EU BACTERIA
AU2005330514B2 (en) 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CA2638763A1 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現

Also Published As

Publication number Publication date
US8114652B2 (en) 2012-02-14
EP2052083A2 (en) 2009-04-29
US8114627B2 (en) 2012-02-14
MX2009002987A (es) 2009-04-28
EP2052083B1 (en) 2014-01-08
CN101516901A (zh) 2009-08-26
PT2052083E (pt) 2014-04-10
US8361770B2 (en) 2013-01-29
BRPI0716963A2 (pt) 2013-11-05
RU2009110152A (ru) 2010-10-27
KR20090074765A (ko) 2009-07-07
CN101516901B (zh) 2014-01-08
PL2052083T3 (pl) 2014-06-30
ES2456331T3 (es) 2014-04-22
US20120184018A1 (en) 2012-07-19
US20090286279A1 (en) 2009-11-19
US20080113407A1 (en) 2008-05-15
WO2008036392A2 (en) 2008-03-27
JP2010504096A (ja) 2010-02-12
EP2052083A4 (en) 2010-03-24
JP5385143B2 (ja) 2014-01-08
WO2008036392A9 (en) 2008-05-22
AU2007297610A1 (en) 2008-03-27
IL197531A0 (en) 2009-12-24
WO2008036392A3 (en) 2008-12-31

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EEER Examination request
FZDE Discontinued

Effective date: 20140922