CN101516901B - 选择性硫酸化的蛋白质在真细菌中的遗传编程表达 - Google Patents

选择性硫酸化的蛋白质在真细菌中的遗传编程表达 Download PDF

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CN101516901B
CN101516901B CN200780035189.6A CN200780035189A CN101516901B CN 101516901 B CN101516901 B CN 101516901B CN 200780035189 A CN200780035189 A CN 200780035189A CN 101516901 B CN101516901 B CN 101516901B
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trna
amino acid
amino acids
unnatural amino
translation system
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CN101516901A (zh
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C·C·刘
P·G·舒尔茨
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Scripps Research Institute
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200780035189.6A 2006-09-21 2007-09-20 选择性硫酸化的蛋白质在真细菌中的遗传编程表达 Expired - Fee Related CN101516901B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US84651906P 2006-09-21 2006-09-21
US60/846,519 2006-09-21
US85521006P 2006-10-28 2006-10-28
US60/855,210 2006-10-28
PCT/US2007/020459 WO2008036392A2 (en) 2006-09-21 2007-09-20 Genetically programmed expression of selectively sulfated proteins in eubacteria

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CN101516901A CN101516901A (zh) 2009-08-26
CN101516901B true CN101516901B (zh) 2014-01-08

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US (3) US8114627B2 (enExample)
EP (1) EP2052083B1 (enExample)
JP (1) JP5385143B2 (enExample)
KR (1) KR20090074765A (enExample)
CN (1) CN101516901B (enExample)
AU (1) AU2007297610A1 (enExample)
BR (1) BRPI0716963A2 (enExample)
CA (1) CA2663052A1 (enExample)
ES (1) ES2456331T3 (enExample)
IL (1) IL197531A0 (enExample)
MX (1) MX2009002987A (enExample)
PL (1) PL2052083T3 (enExample)
PT (1) PT2052083E (enExample)
RU (1) RU2009110152A (enExample)
WO (1) WO2008036392A2 (enExample)

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ES2464532T3 (es) * 2001-04-19 2014-06-03 The Scripps Research Institute Métodos y composiciones para la producción de pares ortogonales de ARNt-aminoacil-ARNt sintetasa
EP1558747B1 (en) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoprotein synthesis
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
US9150849B2 (en) * 2007-11-02 2015-10-06 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN102888387B (zh) * 2011-07-21 2015-03-18 中国科学院生物物理研究所 3-氯代酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
WO2015120287A2 (en) * 2014-02-06 2015-08-13 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2016073079A2 (en) 2014-09-26 2016-05-12 Yale University Compositions and methods for biocontainment of microorganisms
CN110117580B (zh) * 2018-02-05 2021-12-14 中国科学院生物物理研究所 硒代酪氨酸翻译系统及其应用
IL280198B2 (en) * 2018-07-18 2026-01-01 Regenxbio Inc Treatment of mucopolysaccharidosis I with fully glycosylated human iduronidase-alpha-L (idus)
WO2020180971A1 (en) * 2019-03-04 2020-09-10 The Texas A&M University System Methods of making and utilizing amber-obligated phage display libraries
WO2021086487A1 (en) 2019-10-29 2021-05-06 Yale University Engineering organisms resistant to viruses and horizontally transferred genetic elements
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
CN113789335B (zh) * 2021-11-16 2022-03-15 江苏省中国科学院植物研究所 一种水蛭酪氨酸磺酸转移酶基因及其应用
CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204016B1 (en) * 1998-01-29 2001-03-20 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
WO2004035743A2 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1558747B1 (en) 2002-10-16 2009-10-14 The Scripps Research Institute Glycoprotein synthesis
EP1583816A4 (en) 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
EP1704242A4 (en) 2003-07-07 2008-06-18 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LEUCYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
CN101532046A (zh) 2003-10-14 2009-09-16 斯克利普斯研究院 将氧化还原活性氨基酸位点特异地掺入蛋白质
CA2549830A1 (en) 2003-12-18 2006-01-05 The Scripps Research Institute Selective incorporation of 5-hyroxytryptophan into proteins in mammalian cells
CA2567624A1 (en) 2004-05-25 2005-12-08 The Scripps Research Institute Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
US20090181858A1 (en) 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
MX2007003142A (es) 2004-09-21 2007-07-04 Scripps Research Inst Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias.
EP1794312A4 (en) 2004-09-22 2009-08-12 Scripps Research Inst LOCAL LABELING OF PROTEINS FOR NMR STUDIES
WO2006045116A2 (en) 2004-10-20 2006-04-27 The Scripps Research Institute In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
MX2007004892A (es) 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
MX2008011470A (es) 2006-03-09 2008-09-24 Scripps Research Inst Sistemas para la expresion de componentes de traduccion ortogonales en celulas huesped eubacterianas.
US7790847B2 (en) 2006-03-16 2010-09-07 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204016B1 (en) * 1998-01-29 2001-03-20 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
WO2004035743A2 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Wang L et al.Unnatural Amino Acid Mutagenesis of Green Fluorescent Protein.《Journal of Organic Chemistry》.2002,第68卷 *

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RU2009110152A (ru) 2010-10-27
WO2008036392A3 (en) 2008-12-31
JP5385143B2 (ja) 2014-01-08
US20090286279A1 (en) 2009-11-19
AU2007297610A1 (en) 2008-03-27
CN101516901A (zh) 2009-08-26
EP2052083A4 (en) 2010-03-24
WO2008036392A2 (en) 2008-03-27
US20080113407A1 (en) 2008-05-15
US8361770B2 (en) 2013-01-29
US20120184018A1 (en) 2012-07-19
CA2663052A1 (en) 2008-03-27
PL2052083T3 (pl) 2014-06-30
US8114627B2 (en) 2012-02-14
EP2052083B1 (en) 2014-01-08
WO2008036392A9 (en) 2008-05-22
JP2010504096A (ja) 2010-02-12
EP2052083A2 (en) 2009-04-29
IL197531A0 (en) 2009-12-24
KR20090074765A (ko) 2009-07-07
US8114652B2 (en) 2012-02-14
PT2052083E (pt) 2014-04-10
ES2456331T3 (es) 2014-04-22
BRPI0716963A2 (pt) 2013-11-05
MX2009002987A (es) 2009-04-28

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