CA2648557C - A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity - Google Patents

A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity Download PDF

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Publication number
CA2648557C
CA2648557C CA2648557A CA2648557A CA2648557C CA 2648557 C CA2648557 C CA 2648557C CA 2648557 A CA2648557 A CA 2648557A CA 2648557 A CA2648557 A CA 2648557A CA 2648557 C CA2648557 C CA 2648557C
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CA
Canada
Prior art keywords
pair
polynucleotide
subunit
seq
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA2648557A
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English (en)
French (fr)
Other versions
CA2648557A1 (en
Inventor
Juergen Eck
Klaus Liebeton
Lutz Fischer
Sabine Lutz-Wahl
Martina Hensel
Christian Ewert
Marcus Waelz
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BRAIN Biotech AG
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BRAIN Biotechnology Research and Information Network AG
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Publication of CA2648557A1 publication Critical patent/CA2648557A1/en
Application granted granted Critical
Publication of CA2648557C publication Critical patent/CA2648557C/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA2648557A 2006-04-07 2007-04-05 A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity Expired - Fee Related CA2648557C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06007383.0 2006-04-07
EP06007383A EP1842907A1 (en) 2006-04-07 2006-04-07 A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
PCT/EP2007/003114 WO2007115797A2 (en) 2006-04-07 2007-04-05 A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity

Publications (2)

Publication Number Publication Date
CA2648557A1 CA2648557A1 (en) 2007-10-18
CA2648557C true CA2648557C (en) 2017-01-03

Family

ID=36699317

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2648557A Expired - Fee Related CA2648557C (en) 2006-04-07 2007-04-05 A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity

Country Status (7)

Country Link
US (1) US8470576B2 (da)
EP (3) EP1842907A1 (da)
AT (1) ATE480617T1 (da)
CA (1) CA2648557C (da)
DE (1) DE602007009069D1 (da)
DK (2) DK2004814T3 (da)
WO (1) WO2007115797A2 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015203199B2 (en) * 2011-06-07 2017-10-26 Mitsubishi Chemical Corporation Improved nitrile hydratase
KR101698709B1 (ko) 2011-06-07 2017-01-20 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
CN104862296B (zh) * 2015-04-22 2017-12-12 江南大学 一种比酶活和稳定性提高的融合型腈水合酶
RU2736086C1 (ru) * 2016-12-28 2020-11-11 Мицуи Кемикалс, Инк. Мутантная нитрилгидратаза, нуклеиновая кислота, кодирующая указанную мутантную нитрилгидратазу, вектор экспрессии и трансформант, содержащие указанную нуклеиновую кислоту, способ получения указанной мутантной нитрилгидратазы и способ получения амидного соединения
KR20200135010A (ko) 2019-05-24 2020-12-02 (주)아모레퍼시픽 메이크업 상태를 평가하는 방법
KR20230005243A (ko) * 2020-04-24 2023-01-09 파마젤 게엠베하 헤테로사이클릭 알파-아미노 아미드의 위치선택적 산화

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133411A (en) 1966-07-26 2000-10-17 The United States Of America As Represented By The Secretary Of The Navy Slurry mixture forming an additive for producing a hydrodynamic drag reduction aqueous polymer solution
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
AU627648B2 (en) 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US5593871A (en) 1990-09-20 1997-01-14 E. I. Du Pont De Nemours And Company Process for the preparation of enantiometric 2-alkanoic acid amides from nitriles
JPH06303971A (ja) 1993-04-16 1994-11-01 Mitsubishi Kasei Corp ニトリルヒドラターゼ活性を有する新規なタンパク質およびそれをコードする遺伝子ならびに該遺伝子を含有する形質転換体によるニトリル類からアミド類の製造方法
JP3119468B2 (ja) 1994-11-09 2000-12-18 三菱レイヨン株式会社 光学活性なα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法
CA2233868A1 (en) 1995-10-06 1997-04-10 E.I. Du Pont De Nemours And Company Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides
JP3408737B2 (ja) 1998-03-16 2003-05-19 三井化学株式会社 ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子
US20040014195A1 (en) * 1999-12-29 2004-01-22 Diversa Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
WO2002029079A2 (en) * 2000-10-04 2002-04-11 Maxygen, Inc. Enantioselective production of amino carboxylic acids
JP2002325587A (ja) 2001-03-02 2002-11-12 Daicel Chem Ind Ltd ニトリルヒドラターゼ、およびアミドの製造方法
JP2004194588A (ja) 2002-12-19 2004-07-15 Mitsui Chemicals Inc 新規なニトリルヒドラターゼ
DE102004013824A1 (de) 2004-03-20 2005-10-13 Degussa Ag Nitrilhydratasen aus Rhodococcus opacus
DE102004013842A1 (de) 2004-03-20 2005-10-13 Degussa Ag Nitrilhydratasen aus Metagenombibliotheken

Also Published As

Publication number Publication date
EP2261327A2 (en) 2010-12-15
US8470576B2 (en) 2013-06-25
EP2004814A2 (en) 2008-12-24
EP1842907A1 (en) 2007-10-10
CA2648557A1 (en) 2007-10-18
EP2261327B1 (en) 2013-11-20
EP2261327A3 (en) 2011-03-16
DK2004814T3 (da) 2010-12-06
DK2261327T3 (da) 2014-01-13
WO2007115797A2 (en) 2007-10-18
US20100047863A1 (en) 2010-02-25
DE602007009069D1 (de) 2010-10-21
ATE480617T1 (de) 2010-09-15
WO2007115797A3 (en) 2008-03-06
EP2004814B1 (en) 2010-09-08

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