CA2586803A1 - Purification d'immunoglobulines - Google Patents

Purification d'immunoglobulines Download PDF

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Publication number
CA2586803A1
CA2586803A1 CA002586803A CA2586803A CA2586803A1 CA 2586803 A1 CA2586803 A1 CA 2586803A1 CA 002586803 A CA002586803 A CA 002586803A CA 2586803 A CA2586803 A CA 2586803A CA 2586803 A1 CA2586803 A1 CA 2586803A1
Authority
CA
Canada
Prior art keywords
separation matrix
antibodies
matrix according
anyone
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002586803A
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English (en)
Other versions
CA2586803C (fr
Inventor
Hans Berg
Hans J. Johansson
Gunnar Malmquist
Per-Mikael Aberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36588164&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2586803(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from SE0403057A external-priority patent/SE0403057D0/sv
Application filed by Individual filed Critical Individual
Publication of CA2586803A1 publication Critical patent/CA2586803A1/fr
Application granted granted Critical
Publication of CA2586803C publication Critical patent/CA2586803C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28004—Sorbent size or size distribution, e.g. particle size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Fodder In General (AREA)

Abstract

L'invention concerne une matrice de séparation comprenant des particules poreuses sur lesquelles ont été immobilisées des ligands de protéine de liaison aux anticorps, sachant que la densité de ligand se situe dans la gamme 5-10 mg/ml ; le coefficient de distribution de phase de gel des particules exprimé par Kav pour un dextrane ayant une taille de 110 kDa est supérieur à 0,65, et le diamètre de particule moyen se situe dans la gamme 65 - 84 ?m. De préférence, le matériau en hydrate de carbone est de l'agarose hautement réticulé.
CA2586803A 2004-12-14 2005-12-12 Purification d'immunoglobulines Active CA2586803C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE0403057-3 2004-12-14
SE0403057A SE0403057D0 (sv) 2004-12-14 2004-12-14 Purification of immunoglobulins
US63831604P 2004-12-22 2004-12-22
US60/638,316 2004-12-22
PCT/SE2005/001900 WO2006065208A1 (fr) 2004-12-14 2005-12-12 Purification d'immunoglobulines

Publications (2)

Publication Number Publication Date
CA2586803A1 true CA2586803A1 (fr) 2006-06-22
CA2586803C CA2586803C (fr) 2012-12-11

Family

ID=36588164

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2586803A Active CA2586803C (fr) 2004-12-14 2005-12-12 Purification d'immunoglobulines

Country Status (5)

Country Link
EP (1) EP1830947A4 (fr)
JP (1) JP5787461B2 (fr)
AU (1) AU2005317279C1 (fr)
CA (1) CA2586803C (fr)
WO (1) WO2006065208A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110325274A (zh) * 2017-02-27 2019-10-11 通用电气医疗集团生物工艺研发股份公司 分离抗体的分离基质和方法

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006106905A1 (fr) 2005-03-31 2006-10-12 Chugai Seiyaku Kabushiki Kaisha Procede pour la production de polypeptide au moyen de la regulation d’un ensemble
ES2568436T3 (es) 2006-03-31 2016-04-29 Chugai Seiyaku Kabushiki Kaisha Procedimiento para controlar la farmacocinética en sangre de anticuerpos
US9670269B2 (en) 2006-03-31 2017-06-06 Chugai Seiyaku Kabushiki Kaisha Methods of modifying antibodies for purification of bispecific antibodies
CA2664530C (fr) 2006-09-29 2015-11-17 Ge Healthcare Bio-Sciences Ab Ligand de chromatographie comprenant un domaine c issu de la proteine a de staphylococcus aureus pour l'isolement d'anticorps
SG149759A1 (en) * 2007-07-10 2009-02-27 Millipore Corp Media for affinity chromatography
SG10201605394SA (en) 2007-09-26 2016-08-30 Chugai Pharmaceutical Co Ltd Modified Antibody Constant Region
EP3689912A1 (fr) 2007-09-26 2020-08-05 Chugai Seiyaku Kabushiki Kaisha Procédé de modification d'un anticorps par point isoélectrique par substitution d'acide aminé dans cdr
US8277649B2 (en) * 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
JP5889181B2 (ja) 2010-03-04 2016-03-22 中外製薬株式会社 抗体定常領域改変体
EP2542567B1 (fr) 2010-03-05 2020-02-12 Boehringer Ingelheim International Gmbh Enrichissement sélectif d'anticorps
US8859294B2 (en) 2010-03-17 2014-10-14 Panasonic Corporation Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate
CN102388061A (zh) * 2010-03-17 2012-03-21 松下电器产业株式会社 将由a蛋白或a蛋白的a至e结构域中的至少一个结构域所组成的蛋白质结合到基材上的方法
US9090665B2 (en) 2010-03-31 2015-07-28 Jsr Corporation Filler for affinity chromatography
JP5998050B2 (ja) 2010-03-31 2016-09-28 Jsr株式会社 アフィニティークロマトグラフィー用充填剤
MY166429A (en) 2010-11-17 2018-06-26 Chugai Pharmaceutical Co Ltd Multi-specific antigen-binding molecule having alternative function to function of blood coagulation factor viii
CN105518023A (zh) 2013-08-30 2016-04-20 株式会社钟化 Fab区域结合性肽
EP3042192A4 (fr) * 2013-09-04 2017-04-26 Sten Ohlson Chromatographie à faible affinité
JP6230853B2 (ja) * 2013-09-06 2017-11-15 株式会社日立製作所 タンパク質の精製方法及び精製装置
KR102441231B1 (ko) 2013-09-27 2022-09-06 추가이 세이야쿠 가부시키가이샤 폴리펩티드 이종 다량체의 제조방법
JP6387015B2 (ja) 2013-11-27 2018-09-05 Jsr株式会社 固相担体、固相担体の製造方法、アフィニティ精製用担体、アフィニティクロマトグラフィー用充填剤の製造方法、アフィニティクロマトグラフィー用充填剤、クロマトグラフィーカラムおよび精製方法
US10266563B2 (en) 2014-06-24 2019-04-23 Ge Healthcare Bio-Process R&D Ab Chromatography method
WO2015199196A1 (fr) 2014-06-27 2015-12-30 Jsr株式会社 Support pour chromatographie d'affinité
WO2016031902A1 (fr) * 2014-08-28 2016-03-03 株式会社カネカ MATRICE DE SÉPARATION PAR AFFINITÉ POUR UN PEPTIDE CONTENANT UNE RÉGION Fab
JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
JPWO2016121701A1 (ja) 2015-01-26 2017-11-09 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
EP3279216A4 (fr) 2015-04-01 2019-06-19 Chugai Seiyaku Kabushiki Kaisha Procédé pour la production d'un hétéro-oligomère polypeptidique
AU2016381992B2 (en) 2015-12-28 2024-01-04 Chugai Seiyaku Kabushiki Kaisha Method for promoting efficiency of purification of Fc region-containing polypeptide
WO2017195638A1 (fr) 2016-05-09 2017-11-16 株式会社カネカ Procédé de purification d'un anticorps ou d'un fragment d'anticorps comportant une région variable de chaîne κ
JPWO2017195641A1 (ja) * 2016-05-11 2019-03-07 株式会社カネカ アフィニティ分離マトリックスの製造方法およびアフィニティ分離マトリックス

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
US5445732A (en) * 1992-06-19 1995-08-29 Sepracor Inc. Passivated porous polymer supports and methods for the preparation and use of same
WO1994022918A1 (fr) * 1993-03-29 1994-10-13 Minnesota Mining And Manufacturing Company Procede de couplage de ligands au sein de supports poreux (p.e. azlactone) et ses utilisations
SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG
SE9601368D0 (sv) * 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
WO1998030620A1 (fr) * 1997-01-07 1998-07-16 Kaneka Corporation Particules cellulosiques, objets spheriques comportant des particules de polymere reticule et agent adsorbant de purification de fluides corporels
SE9700383D0 (sv) * 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
SE0004929D0 (sv) * 2000-12-29 2000-12-29 Apbiotech Ab A method for producing liquid chromatography matrices
SE0200943D0 (sv) * 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
SE0300482D0 (sv) * 2003-02-21 2003-02-21 Amersham Biosciences Ab Inorganic beads with hierarchical pore structures
US7700743B2 (en) * 2003-07-22 2010-04-20 Millipore Corporation Immobilised affinity medium and its use in separation
US20080312425A1 (en) * 2004-08-30 2008-12-18 Lonza Biologics Plc. Ion Exchange Chromatography and Purification of Antibodies

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110325274A (zh) * 2017-02-27 2019-10-11 通用电气医疗集团生物工艺研发股份公司 分离抗体的分离基质和方法

Also Published As

Publication number Publication date
EP1830947A1 (fr) 2007-09-12
AU2005317279C1 (en) 2014-07-17
EP1830947A4 (fr) 2012-04-18
WO2006065208A1 (fr) 2006-06-22
JP2008523140A (ja) 2008-07-03
JP5787461B2 (ja) 2015-09-30
CA2586803C (fr) 2012-12-11
AU2005317279B2 (en) 2011-02-24
AU2005317279A1 (en) 2006-06-22

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