AU2005317279C1 - Purification of immunoglobulins - Google Patents
Purification of immunoglobulins Download PDFInfo
- Publication number
- AU2005317279C1 AU2005317279C1 AU2005317279A AU2005317279A AU2005317279C1 AU 2005317279 C1 AU2005317279 C1 AU 2005317279C1 AU 2005317279 A AU2005317279 A AU 2005317279A AU 2005317279 A AU2005317279 A AU 2005317279A AU 2005317279 C1 AU2005317279 C1 AU 2005317279C1
- Authority
- AU
- Australia
- Prior art keywords
- separation matrix
- antibodies
- protein
- antibody
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28004—Sorbent size or size distribution, e.g. particle size
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3244—Non-macromolecular compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
- B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0403057-3 | 2004-12-14 | ||
SE0403057A SE0403057D0 (sv) | 2004-12-14 | 2004-12-14 | Purification of immunoglobulins |
US63831604P | 2004-12-22 | 2004-12-22 | |
US60/638,316 | 2004-12-22 | ||
PCT/SE2005/001900 WO2006065208A1 (fr) | 2004-12-14 | 2005-12-12 | Purification d'immunoglobulines |
Publications (3)
Publication Number | Publication Date |
---|---|
AU2005317279A1 AU2005317279A1 (en) | 2006-06-22 |
AU2005317279B2 AU2005317279B2 (en) | 2011-02-24 |
AU2005317279C1 true AU2005317279C1 (en) | 2014-07-17 |
Family
ID=36588164
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2005317279A Active AU2005317279C1 (en) | 2004-12-14 | 2005-12-12 | Purification of immunoglobulins |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1830947A4 (fr) |
JP (1) | JP5787461B2 (fr) |
AU (1) | AU2005317279C1 (fr) |
CA (1) | CA2586803C (fr) |
WO (1) | WO2006065208A1 (fr) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006106905A1 (fr) | 2005-03-31 | 2006-10-12 | Chugai Seiyaku Kabushiki Kaisha | Procede pour la production de polypeptide au moyen de la regulation d’un ensemble |
ES2568436T3 (es) | 2006-03-31 | 2016-04-29 | Chugai Seiyaku Kabushiki Kaisha | Procedimiento para controlar la farmacocinética en sangre de anticuerpos |
US9670269B2 (en) | 2006-03-31 | 2017-06-06 | Chugai Seiyaku Kabushiki Kaisha | Methods of modifying antibodies for purification of bispecific antibodies |
CA2664530C (fr) | 2006-09-29 | 2015-11-17 | Ge Healthcare Bio-Sciences Ab | Ligand de chromatographie comprenant un domaine c issu de la proteine a de staphylococcus aureus pour l'isolement d'anticorps |
SG149759A1 (en) * | 2007-07-10 | 2009-02-27 | Millipore Corp | Media for affinity chromatography |
SG10201605394SA (en) | 2007-09-26 | 2016-08-30 | Chugai Pharmaceutical Co Ltd | Modified Antibody Constant Region |
EP3689912A1 (fr) | 2007-09-26 | 2020-08-05 | Chugai Seiyaku Kabushiki Kaisha | Procédé de modification d'un anticorps par point isoélectrique par substitution d'acide aminé dans cdr |
US8277649B2 (en) * | 2009-12-14 | 2012-10-02 | General Electric Company | Membranes and associated methods for purification of antibodies |
JP5889181B2 (ja) | 2010-03-04 | 2016-03-22 | 中外製薬株式会社 | 抗体定常領域改変体 |
EP2542567B1 (fr) | 2010-03-05 | 2020-02-12 | Boehringer Ingelheim International Gmbh | Enrichissement sélectif d'anticorps |
US8859294B2 (en) | 2010-03-17 | 2014-10-14 | Panasonic Corporation | Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate |
CN102388061A (zh) * | 2010-03-17 | 2012-03-21 | 松下电器产业株式会社 | 将由a蛋白或a蛋白的a至e结构域中的至少一个结构域所组成的蛋白质结合到基材上的方法 |
US9090665B2 (en) | 2010-03-31 | 2015-07-28 | Jsr Corporation | Filler for affinity chromatography |
JP5998050B2 (ja) | 2010-03-31 | 2016-09-28 | Jsr株式会社 | アフィニティークロマトグラフィー用充填剤 |
MY166429A (en) | 2010-11-17 | 2018-06-26 | Chugai Pharmaceutical Co Ltd | Multi-specific antigen-binding molecule having alternative function to function of blood coagulation factor viii |
CN105518023A (zh) | 2013-08-30 | 2016-04-20 | 株式会社钟化 | Fab区域结合性肽 |
EP3042192A4 (fr) * | 2013-09-04 | 2017-04-26 | Sten Ohlson | Chromatographie à faible affinité |
JP6230853B2 (ja) * | 2013-09-06 | 2017-11-15 | 株式会社日立製作所 | タンパク質の精製方法及び精製装置 |
KR102441231B1 (ko) | 2013-09-27 | 2022-09-06 | 추가이 세이야쿠 가부시키가이샤 | 폴리펩티드 이종 다량체의 제조방법 |
JP6387015B2 (ja) | 2013-11-27 | 2018-09-05 | Jsr株式会社 | 固相担体、固相担体の製造方法、アフィニティ精製用担体、アフィニティクロマトグラフィー用充填剤の製造方法、アフィニティクロマトグラフィー用充填剤、クロマトグラフィーカラムおよび精製方法 |
US10266563B2 (en) | 2014-06-24 | 2019-04-23 | Ge Healthcare Bio-Process R&D Ab | Chromatography method |
WO2015199196A1 (fr) | 2014-06-27 | 2015-12-30 | Jsr株式会社 | Support pour chromatographie d'affinité |
WO2016031902A1 (fr) * | 2014-08-28 | 2016-03-03 | 株式会社カネカ | MATRICE DE SÉPARATION PAR AFFINITÉ POUR UN PEPTIDE CONTENANT UNE RÉGION Fab |
JP6663360B2 (ja) | 2015-01-26 | 2020-03-11 | 株式会社カネカ | 変異型免疫グロブリンκ鎖可変領域結合性ペプチド |
JPWO2016121701A1 (ja) | 2015-01-26 | 2017-11-09 | 株式会社カネカ | 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス |
EP3279216A4 (fr) | 2015-04-01 | 2019-06-19 | Chugai Seiyaku Kabushiki Kaisha | Procédé pour la production d'un hétéro-oligomère polypeptidique |
AU2016381992B2 (en) | 2015-12-28 | 2024-01-04 | Chugai Seiyaku Kabushiki Kaisha | Method for promoting efficiency of purification of Fc region-containing polypeptide |
WO2017195638A1 (fr) | 2016-05-09 | 2017-11-16 | 株式会社カネカ | Procédé de purification d'un anticorps ou d'un fragment d'anticorps comportant une région variable de chaîne κ |
JPWO2017195641A1 (ja) * | 2016-05-11 | 2019-03-07 | 株式会社カネカ | アフィニティ分離マトリックスの製造方法およびアフィニティ分離マトリックス |
GB201703116D0 (en) * | 2017-02-27 | 2017-04-12 | Ge Healthcare Bioprocess R&D Ab | A seperation matrix and a method of seperating antibodies |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4444879A (en) * | 1981-01-29 | 1984-04-24 | Science Research Center, Inc. | Immunoassay with article having support film and immunological counterpart of analyte |
US5089605A (en) * | 1987-03-13 | 1992-02-18 | Repligen Corporation | Immobilized immunoglobulin-binding proteins |
US5672276A (en) * | 1992-06-19 | 1997-09-30 | Biosepra Inc. | Passivated porous polymer supports and methods for the preparation and use of same |
US5907016A (en) * | 1993-03-29 | 1999-05-25 | Virginia Tech Intellectual Properties,Inc. | Method of coupling ligands onto supports and products produced therefrom |
EP0955332A1 (fr) * | 1997-01-07 | 1999-11-10 | Kaneka Corporation | Particules cellulosiques, objets spheriques comportant des particules de polymere reticule et agent adsorbant de purification de fluides corporels |
WO2002053254A1 (fr) * | 2000-12-29 | 2002-07-11 | Amersham Biosciences Ab | Methode permettant de produire des matrices de chromatographie liquide |
US6428707B1 (en) * | 1997-02-04 | 2002-08-06 | Amersham Pharmacia Biotech Ab | Adsorption/separation method and a medium for adsorption/separation |
US6602990B1 (en) * | 1996-04-11 | 2003-08-05 | Amersham Biosciences Ab | Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography |
WO2003080655A1 (fr) * | 2002-03-25 | 2003-10-02 | Amersham Biosciences Ab | Proteine de liaison a l'immunoglobuline mutee |
WO2004074211A1 (fr) * | 2003-02-21 | 2004-09-02 | Amersham Biosciences Ab | Perles inorganiques comprenant des structures poreuses hierarchiques |
EP1500431A2 (fr) * | 2003-07-22 | 2005-01-26 | Millipore (U.K.) Ltd. | Agent d'affinité immobilisé et son utilisation pour la séparation |
WO2006024497A1 (fr) * | 2004-08-30 | 2006-03-09 | Lonza Biologics Plc. | Chromatographie par echange d'ions et affinite pour la purification d'anticorps |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE9503925D0 (sv) * | 1995-11-07 | 1995-11-07 | Pharmacia Biotech Ab | Separationsmedium för IgG |
-
2005
- 2005-12-12 CA CA2586803A patent/CA2586803C/fr active Active
- 2005-12-12 EP EP05815565A patent/EP1830947A4/fr not_active Withdrawn
- 2005-12-12 WO PCT/SE2005/001900 patent/WO2006065208A1/fr active Application Filing
- 2005-12-12 AU AU2005317279A patent/AU2005317279C1/en active Active
- 2005-12-12 JP JP2007546603A patent/JP5787461B2/ja active Active
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4444879A (en) * | 1981-01-29 | 1984-04-24 | Science Research Center, Inc. | Immunoassay with article having support film and immunological counterpart of analyte |
US5089605A (en) * | 1987-03-13 | 1992-02-18 | Repligen Corporation | Immobilized immunoglobulin-binding proteins |
US5672276A (en) * | 1992-06-19 | 1997-09-30 | Biosepra Inc. | Passivated porous polymer supports and methods for the preparation and use of same |
US5907016A (en) * | 1993-03-29 | 1999-05-25 | Virginia Tech Intellectual Properties,Inc. | Method of coupling ligands onto supports and products produced therefrom |
US6602990B1 (en) * | 1996-04-11 | 2003-08-05 | Amersham Biosciences Ab | Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography |
EP0955332A1 (fr) * | 1997-01-07 | 1999-11-10 | Kaneka Corporation | Particules cellulosiques, objets spheriques comportant des particules de polymere reticule et agent adsorbant de purification de fluides corporels |
US6428707B1 (en) * | 1997-02-04 | 2002-08-06 | Amersham Pharmacia Biotech Ab | Adsorption/separation method and a medium for adsorption/separation |
WO2002053254A1 (fr) * | 2000-12-29 | 2002-07-11 | Amersham Biosciences Ab | Methode permettant de produire des matrices de chromatographie liquide |
WO2003080655A1 (fr) * | 2002-03-25 | 2003-10-02 | Amersham Biosciences Ab | Proteine de liaison a l'immunoglobuline mutee |
WO2004074211A1 (fr) * | 2003-02-21 | 2004-09-02 | Amersham Biosciences Ab | Perles inorganiques comprenant des structures poreuses hierarchiques |
EP1500431A2 (fr) * | 2003-07-22 | 2005-01-26 | Millipore (U.K.) Ltd. | Agent d'affinité immobilisé et son utilisation pour la séparation |
WO2006024497A1 (fr) * | 2004-08-30 | 2006-03-09 | Lonza Biologics Plc. | Chromatographie par echange d'ions et affinite pour la purification d'anticorps |
Also Published As
Publication number | Publication date |
---|---|
EP1830947A1 (fr) | 2007-09-12 |
CA2586803A1 (fr) | 2006-06-22 |
EP1830947A4 (fr) | 2012-04-18 |
WO2006065208A1 (fr) | 2006-06-22 |
JP2008523140A (ja) | 2008-07-03 |
JP5787461B2 (ja) | 2015-09-30 |
CA2586803C (fr) | 2012-12-11 |
AU2005317279B2 (en) | 2011-02-24 |
AU2005317279A1 (en) | 2006-06-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
CB | Opposition filed |
Opponent name: STEPHEN DEAN SHARP AND DAVID JOHN HUGHES |
|
ON | Decision of a delegate or deputy of the commissioner of patents (result of patent office hearing) |
Free format text: APO 10: LACK OF CLARITY NOT ESTABLISHED - FAIR BASIS, CLAIMS NEED NOT BE LIMITED TO THE PREFERRED EMBODIMENTS - LACK OF FAIR BASIS NOT ESTABLISHED. Opponent name: STEPHEN DEAN SHARP DAVID JOHN HUGHES |
|
DA2 | Applications for amendment section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 13 MAR 2014 . |
|
DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 13 MAR 2014 |
|
FGA | Letters patent sealed or granted (standard patent) | ||
PC | Assignment registered |
Owner name: GE HEALTHCARE BIOPROCESS R&D AB Free format text: FORMER OWNER WAS: GE HEALTHCARE BIO-SCIENCES AB |
|
HB | Alteration of name in register |
Owner name: CYTIVA BIOPROCESS R&D AB Free format text: FORMER NAME(S): GE HEALTHCARE BIOPROCESS R&D AB |