CA2585404A1 - Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms - Google Patents

Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms Download PDF

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Publication number
CA2585404A1
CA2585404A1 CA002585404A CA2585404A CA2585404A1 CA 2585404 A1 CA2585404 A1 CA 2585404A1 CA 002585404 A CA002585404 A CA 002585404A CA 2585404 A CA2585404 A CA 2585404A CA 2585404 A1 CA2585404 A1 CA 2585404A1
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CA
Canada
Prior art keywords
seq
gene
sequence
nucleic acid
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002585404A
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English (en)
French (fr)
Inventor
Narendra S. Yadav
Zhixiong Xue
Hongxiang Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2585404A1 publication Critical patent/CA2585404A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Edible Oils And Fats (AREA)
  • Fats And Perfumes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002585404A 2004-11-04 2005-11-03 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms Abandoned CA2585404A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US62481204P 2004-11-04 2004-11-04
US60/624,812 2004-11-04
US11/024,544 2004-12-29
US11/024,544 US7273746B2 (en) 2004-11-04 2004-12-29 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
PCT/US2005/040345 WO2006052914A2 (en) 2004-11-04 2005-11-03 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms

Publications (1)

Publication Number Publication Date
CA2585404A1 true CA2585404A1 (en) 2006-05-18

Family

ID=36262489

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002585404A Abandoned CA2585404A1 (en) 2004-11-04 2005-11-03 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms

Country Status (5)

Country Link
US (3) US7273746B2 (enExample)
EP (1) EP1807525A4 (enExample)
JP (1) JP2008524984A (enExample)
CA (1) CA2585404A1 (enExample)
WO (2) WO2006052912A2 (enExample)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1756280T3 (pl) 2004-04-22 2015-04-30 Commw Scient Ind Res Org Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
AU2005250074B2 (en) * 2004-06-04 2011-04-14 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
BRPI0716075A2 (pt) 2006-08-29 2013-08-06 Commw Scient Ind Res Org sÍntese de Ácidos graxos
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
EP2730656A1 (en) 2007-05-24 2014-05-14 E. I. du Pont de Nemours and Company Soybean meal from beans having DGAT genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
CA2701130A1 (en) * 2007-10-03 2009-04-09 E.I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms
EP2620501A3 (en) * 2008-05-23 2013-10-02 E. I. du Pont de Nemours and Company DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
EP2282629A1 (en) 2008-05-23 2011-02-16 E. I. du Pont de Nemours and Company Dgat genes from yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
EP2324120B1 (en) 2008-08-29 2016-01-27 E. I. du Pont de Nemours and Company Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms
CA3015426C (en) 2008-11-18 2020-01-14 Commonwealth Scientific And Industrial Research Organisation Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases
US8431772B1 (en) 2008-11-19 2013-04-30 University Of Kentucky Research Foundation Diacylglycerol acyltransferase sequences and related methods
US8993840B2 (en) 2009-11-23 2015-03-31 E I du Pont de Nemours and Compay Sucrose transporter genes for increasing plant seed lipids
CN102844431B (zh) 2009-12-21 2014-10-08 三得利控股株式会社 二酰甘油酰基转移酶基因及其用途
EP3521408B1 (en) 2010-11-03 2021-12-22 Corbion Biotech, Inc. Genetically-engineered chlorella or prototheca microbe and oil produced therefrom
US8969049B2 (en) 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
EP2714724A1 (en) 2011-05-26 2014-04-09 E. I. Du Pont de Nemours and Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
EP3388516A1 (en) * 2011-10-19 2018-10-17 Massachusetts Institute Of Technology Engineered microbes and methods for microbial oil production
WO2013096562A1 (en) 2011-12-22 2013-06-27 E. I. Du Pont De Nemours And Company Use of the soybean sucrose synthase promoter to increase plant seed lipid content
HUE033766T2 (en) 2012-06-15 2018-01-29 Commw Scient Ind Res Org Production of long chain polyunsaturated fatty acids in plant cells
BR112015002940A2 (pt) 2012-08-10 2018-04-24 Opx Biotechnologies Inc microorganismos e métodos para a produção de ácidos graxos e produtos derivados de ácidos graxos.
WO2014062163A1 (en) 2012-10-16 2014-04-24 Exxonmobil Research And Engineering Company Dgat genes and methods of use for triglyceride production in recombinant microorganisms
BR112015009303A2 (pt) * 2012-10-30 2017-08-22 Agresearch Ltd Novos polipeptídeos, polinucleotídeos de aciltransferase, e métodos de uso
PL2914726T3 (pl) 2012-10-30 2019-06-28 Agresearch Limited Udoskonalone polinukleotydy acylotransferazy, polipeptydy i sposoby stosowania
CN105473718A (zh) 2012-10-30 2016-04-06 农业研究有限公司 增强的酰基转移酶的多核苷酸、多肽、及其使用方法
EP2929040A4 (en) 2012-12-06 2016-11-09 Exxonmobil Res & Eng Co DGAT GENES WITH PLECKSTRIN HOMOLOGY DOMAINS AND USE IN RECOMBINANT MICROORGANISMS
CN109576241A (zh) 2012-12-21 2019-04-05 新西兰植物和食品研究院有限公司 基因表达的调控
US9447438B2 (en) 2013-03-15 2016-09-20 Cargill, Incorporated Acetyl-coA carboxylases
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
WO2015010103A2 (en) 2013-07-19 2015-01-22 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
EA037817B1 (ru) 2013-12-18 2021-05-25 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты
AU2015218647B2 (en) 2014-02-24 2021-10-21 Ginkgo Bioworks, Inc. Diacylglycerol acyl transferase (DGA1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous DGA1
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2018047657A1 (ja) * 2016-09-07 2018-03-15 花王株式会社 脂質の製造方法
WO2018140782A1 (en) 2017-01-27 2018-08-02 Marks Michael David Plants having increased oil quality
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞
US11337391B2 (en) 2017-08-18 2022-05-24 Regents Of The University Of Minnesota Early flowering plants
US11707029B2 (en) 2017-08-18 2023-07-25 Regents Of The University Of Minnesota Oilseed plants having reduced pod shatter
US12221612B2 (en) 2018-02-12 2025-02-11 Regents Of The University Of Minnesota Plants having increased oil quality
CN109837256B (zh) * 2019-03-21 2020-12-01 江南大学 一种二酰甘油酰基转移酶1及其在生产甘油三酯中的应用
US11408008B2 (en) * 2019-03-28 2022-08-09 Regents Of The University Of Minnesota Plants having increased oil quality
CN114517164A (zh) * 2020-11-19 2022-05-20 中国科学院青岛生物能源与过程研究所 一种脂肪酸延长酶基因在酵母合成神经酸中的应用
MX2023010280A (es) * 2021-03-03 2023-09-11 Nourish Ingredients Pty Ltd Produccion de fosfolipidos en microbios y usos de los mismos.
CN116396875A (zh) * 2022-12-20 2023-07-07 华东理工大学 一种解脂耶氏酵母基因工程菌及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4792523A (en) 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
US6432684B1 (en) * 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US20030167483A1 (en) * 1998-06-24 2003-09-04 Farese Robert V. Diacylglycerol O-acyltransferase
US6344548B1 (en) * 1998-06-24 2002-02-05 The Regents Of The University Of California Diacylglycerol o-acyltransferase
US6100077A (en) * 1998-10-01 2000-08-08 The Trustees Of Columbia University In The City Of New York Isolation of a gene encoding diacylglycerol acyltransferase
CA2851896C (en) * 1998-12-02 2016-05-17 Karlene H. Butler Plant diacylglycerol acyltransferases
HUP0200480A3 (en) 1999-04-01 2009-08-28 Basf Plant Science Gmbh Enzymes of the biosynthetic pathway for the production of triacylglycerol and recombinant dna molecules encoding these enzymes
DE19950921A1 (de) * 1999-10-21 2001-04-26 Basf Ag The N-terminal beta-barrel Structur der Lipidkörperlipoxygenase vermittelt ihre Bindung an Liposomen und Lipidkörper
US6552250B1 (en) * 2000-06-14 2003-04-22 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Diacylglycerol O-acyltransferase
SE0201581D0 (sv) * 2002-05-29 2002-05-29 Scandinavian Biotechnology Res New improved acyltransferase
CA2492205A1 (en) 2002-07-31 2004-02-05 Monsanto Technology, Llc Diacylglycerol acyltransferase nucleic acid sequences and associated products
EP2431048B1 (en) * 2002-10-08 2015-03-11 Danisco US Inc. Phenolic binding peptides
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts

Also Published As

Publication number Publication date
JP2008524984A (ja) 2008-07-17
WO2006052914A3 (en) 2009-02-19
US7273746B2 (en) 2007-09-25
US20060094086A1 (en) 2006-05-04
EP1807525A4 (en) 2010-02-17
US20090104674A1 (en) 2009-04-23
WO2006052912A2 (en) 2006-05-18
WO2006052912A3 (en) 2009-04-09
US8685681B2 (en) 2014-04-01
US20080020415A1 (en) 2008-01-24
WO2006052914A2 (en) 2006-05-18
EP1807525A2 (en) 2007-07-18

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20141003