CA2576804C - A method of analysing a sample and apparatus therefor - Google Patents

A method of analysing a sample and apparatus therefor Download PDF

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Publication number
CA2576804C
CA2576804C CA2576804A CA2576804A CA2576804C CA 2576804 C CA2576804 C CA 2576804C CA 2576804 A CA2576804 A CA 2576804A CA 2576804 A CA2576804 A CA 2576804A CA 2576804 C CA2576804 C CA 2576804C
Authority
CA
Canada
Prior art keywords
sample
modification
labels
processing means
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2576804A
Other languages
English (en)
French (fr)
Other versions
CA2576804A1 (en
Inventor
Colin Blackmore
Patrick Courtney
Alistair Fitch
Shab Ladha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Singapore Pte Ltd
Original Assignee
PerkinElmer Singapore Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0419325.6A external-priority patent/GB0419325D0/en
Application filed by PerkinElmer Singapore Pte Ltd filed Critical PerkinElmer Singapore Pte Ltd
Publication of CA2576804A1 publication Critical patent/CA2576804A1/en
Application granted granted Critical
Publication of CA2576804C publication Critical patent/CA2576804C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA2576804A 2004-09-01 2005-08-17 A method of analysing a sample and apparatus therefor Expired - Lifetime CA2576804C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0419325.6 2004-09-01
GBGB0419325.6A GB0419325D0 (en) 2004-09-01 2004-09-01 A method of analysing a sample including fluorescent labels and apparatus therefor
GB0512811A GB2418018B (en) 2004-09-01 2005-06-23 A method of analysing a sample including fluorescent labels and apparatus therefor
GB0512811.1 2005-06-23
PCT/GB2005/003226 WO2006024819A2 (en) 2004-09-01 2005-08-17 A method of analysing a sample and apparatus therefor

Publications (2)

Publication Number Publication Date
CA2576804A1 CA2576804A1 (en) 2006-03-09
CA2576804C true CA2576804C (en) 2013-10-15

Family

ID=35079173

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2576804A Expired - Lifetime CA2576804C (en) 2004-09-01 2005-08-17 A method of analysing a sample and apparatus therefor

Country Status (6)

Country Link
EP (1) EP1784633B1 (enExample)
JP (1) JP5318413B2 (enExample)
KR (1) KR101286216B1 (enExample)
AU (1) AU2005279041B2 (enExample)
CA (1) CA2576804C (enExample)
WO (1) WO2006024819A2 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4855139B2 (ja) 2006-05-18 2012-01-18 オリンパス株式会社 顕微鏡装置および細胞観察方法
US8664589B2 (en) * 2011-12-29 2014-03-04 Electro Scientific Industries, Inc Spectroscopy data display systems and methods
WO2014168734A1 (en) * 2013-03-15 2014-10-16 Cedars-Sinai Medical Center Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof
WO2017173315A1 (en) 2016-04-01 2017-10-05 Black Light Surgical, Inc. Systems, devices, and methods for time-resolved fluorescent spectroscopy
DE102016109303A1 (de) * 2016-05-20 2017-11-23 Friedrich-Schiller-Universität Jena Lasermikroskop mit Ablationsfunktion
JP6033980B1 (ja) * 2016-06-01 2016-11-30 株式会社片岡製作所 細胞処理システム
JP6541160B2 (ja) 2017-02-13 2019-07-10 株式会社片岡製作所 細胞処理装置および対象物の処理方法
JP6532063B2 (ja) 2017-02-13 2019-06-19 株式会社片岡製作所 細胞処理装置
JP6574028B1 (ja) 2018-06-29 2019-09-11 株式会社片岡製作所 細胞処理装置および細胞のレーザ処理方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629687A (en) * 1982-07-29 1986-12-16 Board Of Trustees Of Michigan State University Positive selection sorting of cells
US5932872A (en) * 1994-07-01 1999-08-03 Jeffrey H. Price Autofocus system for scanning microscopy having a volume image formation
FI101829B (fi) * 1995-03-07 1998-08-31 Erkki Juhani Soini Biospesifinen määritysmenetelmä
FI98765C (fi) * 1995-01-16 1997-08-11 Erkki Soini Virtaussytometrinen menetelmä ja laite
ATE244879T1 (de) * 1997-04-25 2003-07-15 Univ Amsterdam Photobleichbare lumineszenzschichten zur kalibrierung und standardisierung in optischer mikroskopie
JP3735190B2 (ja) * 1997-10-28 2006-01-18 オリンパス株式会社 走査型サイトメータ
JPH11271220A (ja) * 1998-03-24 1999-10-05 Olympus Optical Co Ltd 走査型顕微鏡の測定パラメータ決定方法
AU5223899A (en) * 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
JP3678397B2 (ja) * 1998-12-15 2005-08-03 富士写真フイルム株式会社 撮影システム
US20030026762A1 (en) * 1999-05-05 2003-02-06 Malmros Mark K. Bio-spectral imaging system and methods for diagnosing cell disease state
EP1187555A4 (en) * 1999-05-26 2004-12-01 Photogen Inc IMPROVED METHOD AND DEVICE FOR MULTIPHOTON PHOTOACTIVATION AND DETERMINATION OF MOLECULAR SUBSTANCES
ATE249657T1 (de) * 2000-04-06 2003-09-15 European Molecular Biology Lab Embl Rechnergesteuertes mikroskop
WO2002048693A1 (fr) * 2000-12-14 2002-06-20 Olympus Optical Co., Ltd. Analyseur fluorometrique et analyse fluorometrique
JP3734769B2 (ja) * 2001-11-09 2006-01-11 アイダエンジニアリング株式会社 蛍光強度測定方法および装置
GB0224067D0 (en) * 2002-10-16 2002-11-27 Perkinelmer Uk Ltd Improvements in and relating to imaging
EP1565714A4 (en) * 2002-11-18 2009-06-03 Panomics Inc ORDERING uncaging

Also Published As

Publication number Publication date
JP2008511824A (ja) 2008-04-17
EP1784633B1 (en) 2011-11-09
WO2006024819A3 (en) 2006-05-18
KR20070050085A (ko) 2007-05-14
KR101286216B1 (ko) 2013-07-15
JP5318413B2 (ja) 2013-10-16
AU2005279041A1 (en) 2006-03-09
CA2576804A1 (en) 2006-03-09
EP1784633A2 (en) 2007-05-16
AU2005279041B2 (en) 2011-04-07
WO2006024819A2 (en) 2006-03-09
WO2006024819A8 (en) 2006-09-08

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