CA2575994A1 - Optimizing expression of active botulinum toxin type a - Google Patents
Optimizing expression of active botulinum toxin type a Download PDFInfo
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- CA2575994A1 CA2575994A1 CA002575994A CA2575994A CA2575994A1 CA 2575994 A1 CA2575994 A1 CA 2575994A1 CA 002575994 A CA002575994 A CA 002575994A CA 2575994 A CA2575994 A CA 2575994A CA 2575994 A1 CA2575994 A1 CA 2575994A1
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- QSHGUCSTWRSQAF-FJSLEGQWSA-N s-peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@@H](NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C1=CC=C(OS(O)(=O)=O)C=C1 QSHGUCSTWRSQAF-FJSLEGQWSA-N 0.000 description 1
- 238000003345 scintillation counting Methods 0.000 description 1
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- 230000001953 sensory effect Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
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- 238000002741 site-directed mutagenesis Methods 0.000 description 1
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- 239000002689 soil Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 201000002849 spasmodic dystonia Diseases 0.000 description 1
- 208000018198 spasticity Diseases 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003153 stable transfection Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000013179 statistical model Methods 0.000 description 1
- 108010018381 streptavidin-binding peptide Proteins 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 230000000946 synaptic effect Effects 0.000 description 1
- 210000002504 synaptic vesicle Anatomy 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
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- 150000003522 tetracyclines Chemical class 0.000 description 1
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- 238000012876 topography Methods 0.000 description 1
- 238000006257 total synthesis reaction Methods 0.000 description 1
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- 230000001988 toxicity Effects 0.000 description 1
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- 230000013715 transcription antitermination Effects 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
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- 230000001131 transforming effect Effects 0.000 description 1
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- 230000010474 transient expression Effects 0.000 description 1
- 238000003146 transient transfection Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US59912104P | 2004-08-04 | 2004-08-04 | |
| US60/599,121 | 2004-08-04 | ||
| PCT/US2005/027917 WO2006017749A2 (en) | 2004-08-04 | 2005-08-03 | Optimizing expression of active botulinum toxin type a |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2575994A1 true CA2575994A1 (en) | 2006-02-16 |
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| CA002575994A Abandoned CA2575994A1 (en) | 2004-08-04 | 2005-08-03 | Optimizing expression of active botulinum toxin type a |
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| CA (1) | CA2575994A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2006017749A2 (cg-RX-API-DMAC7.html) |
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| US7514088B2 (en) | 2005-03-15 | 2009-04-07 | Allergan, Inc. | Multivalent Clostridial toxin derivatives and methods of their use |
| US20080057575A1 (en) * | 2004-08-04 | 2008-03-06 | Allergan, Inc. | Optimizing Expression of Active Botulinum Toxin Type A |
| CA2588758C (en) * | 2004-11-22 | 2017-01-03 | New York University | Genetically engineered clostridial genes, proteins encoded by the engineered genes, and uses thereof |
| ES2341892T5 (es) | 2005-09-19 | 2019-03-27 | Allergan Inc | Toxina clostridial activable por toxinas clostridiales |
| US8168206B1 (en) | 2005-10-06 | 2012-05-01 | Allergan, Inc. | Animal protein-free pharmaceutical compositions |
| US20070292918A1 (en) * | 2006-05-30 | 2007-12-20 | Stelman Steven J | Codon optimization method |
| EP2167119B1 (en) | 2007-06-14 | 2016-08-24 | The Secretary of State for Health | Chemically modified peptides with improved immunogenicity |
| CN102307571A (zh) | 2008-12-10 | 2012-01-04 | 阿勒根公司 | 梭菌毒素药物组合物 |
| EP2218783A1 (en) | 2009-02-05 | 2010-08-18 | Merz Pharma GmbH & Co. KGaA | Novel method for the manufacturing of neurotoxins |
| CN102422158B (zh) | 2009-03-13 | 2015-04-08 | 阿勒根公司 | 基于免疫的重靶向内肽酶活性测定 |
| SG181772A1 (en) | 2009-12-16 | 2012-07-30 | Allergan Inc | Modified clostridial toxins comprising an integrated protease cleavage site-binding domain |
| EP2684890B1 (en) | 2010-01-25 | 2016-04-20 | Allergan, Inc. | Methods of intracellular conversion of single-chain proteins into their di-chain form |
| EP2528941A4 (en) * | 2010-01-25 | 2013-05-29 | Univ New York | FOR TRANSPORT STUDIES AND NEURONAL ADMINISTRATION, MANIPULATED RECOMBINANT DERIVATIVES FROM BOTULINUM NEUROTOXINES |
| ES2553149T3 (es) | 2010-11-23 | 2015-12-04 | Allergan, Inc. | Composiciones y métodos para producir enterocinasa en la levadura |
| US20120244188A1 (en) | 2011-03-25 | 2012-09-27 | Allergan, Inc. | Treatment of Sensory Disturbance Disorders |
| US20120251574A1 (en) | 2011-03-28 | 2012-10-04 | Allergan, Inc. | Endopeptidase and Neurotoxin Combination Treatment of Multiple Medical Conditions |
| US20120251573A1 (en) | 2011-03-28 | 2012-10-04 | Allergan, Inc. | Endopeptidase Treatment of Neuroendocrine Disorders |
| US20120251575A1 (en) | 2011-03-28 | 2012-10-04 | Allergan, Inc. | Endopeptidase Treatment of Involuntary Movement Disorders |
| US20120258132A1 (en) | 2011-03-29 | 2012-10-11 | Allergan, Inc. | Vagal Nerve-Based Disorders |
| US20120251519A1 (en) | 2011-03-29 | 2012-10-04 | Allergan, Inc. | Endopeptidase Treatment of Smooth Muscle Disorders |
| US20120251515A1 (en) | 2011-03-29 | 2012-10-04 | Allergan, Inc. | Endopeptidase Treatment of Cosmesis Disorders |
| US20120251518A1 (en) | 2011-03-29 | 2012-10-04 | Allergan, Inc. | Endopeptidase Treatment of Sexual Dysfunction Disorders |
| AU2012255746B2 (en) * | 2011-05-17 | 2016-04-28 | Boston Scientific Neuromodulation Corporation | User-defined graphical shapes used as a visualization aid for stimulator programming |
| WO2012174123A1 (en) | 2011-06-13 | 2012-12-20 | Allergan, Inc. | Treatment of psychological trauma |
| US20140377248A1 (en) | 2011-12-23 | 2014-12-25 | Merz Pharma Gmbh & Co. Kgaa | Method for the manufacturing of di-chain proteins for use in humans |
| US20130171122A1 (en) | 2011-12-29 | 2013-07-04 | Allergan, Inc. | Endopeptidase and neurotoxin combination treatment of bladder disorders |
| RU2673910C2 (ru) | 2012-11-21 | 2018-12-03 | Ипсен Байоинновейшн Лимитед | Способ производства протеолитически процессированного полипептида |
| EP2948174B1 (en) | 2013-01-28 | 2019-09-11 | New York University | Treatment methods using atoxic neurotoxin derivatives |
| KR20150127585A (ko) | 2013-03-15 | 2015-11-17 | 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 | 보툴리누스균의 혈청형으로부터의 중쇄를 사용한 1가 또는 다가 보툴리늄 신경독소 기반 백신 |
| WO2015132004A1 (en) | 2014-03-05 | 2015-09-11 | Merz Pharma Gmbh & Co. Kgaa | Novel recombinant clostridial neurotoxins with increased duration of effect |
| EP3230457B1 (en) | 2014-12-09 | 2021-06-30 | New York University | Clostridial neurotoxin fusion proteins, propeptide fusions, their expression, and use |
| EP3233911B1 (en) | 2014-12-19 | 2020-10-14 | Merz Pharma GmbH & Co. KGaA | Means and methods for the determination of the biological activity of bont/e in cells |
| PT3242884T (pt) * | 2015-01-09 | 2021-04-22 | Ipsen Bioinnovation Ltd | Neurotoxinas catiónicas |
| TW201718627A (zh) | 2015-06-11 | 2017-06-01 | 梅茲製藥有限兩合公司 | 重組梭菌神經毒素及其使用與形成方法、包括其之醫藥組合物及對應其之前驅物、編碼前驅物之核酸序列及其獲得方法與前驅物之形成方法、載體與包括核酸序列之重組宿主細胞 |
| US11078472B2 (en) | 2016-01-20 | 2021-08-03 | Merz Pharma Gmbh & Co., Kgaa | Recombinant clostridial neurotoxins with increased duration of effect |
| US11969461B2 (en) | 2016-03-02 | 2024-04-30 | Merz Pharma Gmbh & Co. Kgaa | Composition comprising botulinum toxin |
| EP3290437A1 (en) | 2016-08-31 | 2018-03-07 | Merz Pharma GmbH & Co. KGaA | Novel recombinant clostridial neurotoxins with decreased duration of effect |
| PL3432916T3 (pl) | 2016-09-13 | 2020-04-30 | Allergan, Inc. | Niebiałkowo stabilzowane kompozycje toksyny clostridium |
| EP3312193A1 (en) | 2016-10-19 | 2018-04-25 | Merz Pharma GmbH & Co. KGaA | Novel recombinant botulinum neurotoxins with accelerated onset of effect |
| EP3333179A1 (en) | 2016-12-07 | 2018-06-13 | Merz Pharma GmbH & Co. KGaA | Novel recombinant botulinum toxin with accelarated onset of effect |
| EP3335719A1 (en) | 2016-12-14 | 2018-06-20 | Merz Pharma GmbH & Co. KGaA | Novel recombinant botulinum neurotoxins with a stabilized light chain |
| EP3642222A1 (en) | 2017-06-20 | 2020-04-29 | Merz Pharma GmbH & Co. KGaA | Novel recombinant botulinum toxin with increased duration of effect |
| EP3649143B1 (en) | 2017-07-06 | 2022-08-31 | Merz Pharma GmbH & Co. KGaA | Novel recombinant botulinum neurotoxins with increased duration of effect |
| US20210008156A1 (en) | 2017-10-26 | 2021-01-14 | Merz Pharma Gmbh & Co. Kgaa | Novel recombinant botulinum neurotoxins with increased duration of effect |
| US20200354706A1 (en) | 2017-11-22 | 2020-11-12 | Merz Pharma Gmbh & Co. Kgaa | Novel recombinant botulinum toxin with increased duration of effect |
| WO2019152380A1 (en) * | 2018-01-30 | 2019-08-08 | Children's Medical Center Corporation | Production of botulinum neurotoxins using bacillus systems |
| US11707510B2 (en) * | 2018-02-16 | 2023-07-25 | Preclinics Discovery Gmbh | Nucleic acid-based botulinum neurotoxin for therapeutic use |
| WO2020174079A1 (en) * | 2019-02-28 | 2020-09-03 | Proteonic Biotechnology Ip B.V. | Self-immolative plasmid backbone |
| WO2022197623A1 (en) * | 2021-03-15 | 2022-09-22 | Children's Medical Center Corporation | Engineered botulinum neurotoxin a protease domain with improved efficacy |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2003383A1 (en) * | 1988-11-23 | 1990-05-23 | Sushil G. Devare | Synthetic dna derived recombinant hiv antigens |
| UA48104C2 (uk) * | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
| US7037680B2 (en) * | 1993-09-21 | 2006-05-02 | The United States Of America As Represented By The Secretary Of The Army | Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy |
| US7214787B1 (en) * | 1993-09-21 | 2007-05-08 | United States Of America As Represented By The Secretary Of The Army | Recombinant vaccine against botulinum neurotoxin |
| NZ337543A (en) * | 1994-10-24 | 2002-06-28 | Allergan Inc | Composition comprising avian antitoxin against clostridial neurotoxin protein and method for producing recombinant antitoxin proteins are provided for |
| US6114148C1 (en) * | 1996-09-20 | 2012-05-01 | Gen Hospital Corp | High level expression of proteins |
| EP1020517A4 (en) * | 1997-07-11 | 2004-11-17 | Chugai Pharmaceutical Co Ltd | A HUMAN CHONDROCYTE GENE |
| US6277622B1 (en) * | 1997-08-11 | 2001-08-21 | The University Of Sydney | Synthetic polynucleotides |
| US6218188B1 (en) * | 1997-11-12 | 2001-04-17 | Mycogen Corporation | Plant-optimized genes encoding pesticidal toxins |
| US6214602B1 (en) * | 1998-08-28 | 2001-04-10 | Promega Corporation | Host cells for expression of clostridial toxins and proteins |
| JP2004512004A (ja) * | 1999-05-12 | 2004-04-22 | ユナイテッド ステイツ アーミー メディカル リサーチ アンド マテリエル シーエムディー | ボツリヌス神経毒に対する組換えワクチン |
| US6399857B1 (en) * | 1999-09-22 | 2002-06-04 | Paradigm Genetics, Inc. | Modified nucleotide sequence encoding a LexA DNA binding domain optimized for arabidopsis species |
| AU2002211524B2 (en) * | 2000-10-04 | 2007-03-22 | The Trustees Of The University Of Pennsylvania | Highly expressible genes |
| TW200403338A (en) * | 2002-07-16 | 2004-03-01 | Advisys Inc | Codon optimized synthetic plasmids |
| US20080057575A1 (en) * | 2004-08-04 | 2008-03-06 | Allergan, Inc. | Optimizing Expression of Active Botulinum Toxin Type A |
-
2005
- 2005-08-03 US US11/570,706 patent/US20080057575A1/en not_active Abandoned
- 2005-08-03 WO PCT/US2005/027917 patent/WO2006017749A2/en not_active Ceased
- 2005-08-03 AU AU2005271372A patent/AU2005271372B2/en not_active Ceased
- 2005-08-03 EP EP05778839A patent/EP1773874B1/en not_active Revoked
- 2005-08-03 JP JP2007525034A patent/JP2008508886A/ja active Pending
- 2005-08-03 CA CA002575994A patent/CA2575994A1/en not_active Abandoned
-
2008
- 2008-08-15 US US12/192,873 patent/US20090023198A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1773874B1 (en) | 2012-10-24 |
| JP2008508886A (ja) | 2008-03-27 |
| WO2006017749A2 (en) | 2006-02-16 |
| US20090023198A1 (en) | 2009-01-22 |
| AU2005271372A1 (en) | 2006-02-16 |
| WO2006017749A3 (en) | 2006-03-30 |
| EP1773874A2 (en) | 2007-04-18 |
| AU2005271372B2 (en) | 2012-05-03 |
| US20080057575A1 (en) | 2008-03-06 |
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