CA2562171A1 - Mutant .alpha.-amylases - Google Patents

Mutant .alpha.-amylases Download PDF

Info

Publication number
CA2562171A1
CA2562171A1 CA002562171A CA2562171A CA2562171A1 CA 2562171 A1 CA2562171 A1 CA 2562171A1 CA 002562171 A CA002562171 A CA 002562171A CA 2562171 A CA2562171 A CA 2562171A CA 2562171 A1 CA2562171 A1 CA 2562171A1
Authority
CA
Canada
Prior art keywords
amylase
alpha
variant
amino acid
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002562171A
Other languages
English (en)
French (fr)
Inventor
Eugenio Ferrari
Marc Kolkman
Craig E. Pilgrim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2562171A1 publication Critical patent/CA2562171A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618Protease or amylase in liquid compositions only

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CA002562171A 2004-04-08 2005-04-08 Mutant .alpha.-amylases Abandoned CA2562171A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56112404P 2004-04-08 2004-04-08
US60/561,124 2004-04-08
PCT/US2005/012074 WO2005111203A2 (en) 2004-04-08 2005-04-08 MUTANT α ΑMYLASES

Publications (1)

Publication Number Publication Date
CA2562171A1 true CA2562171A1 (en) 2005-11-24

Family

ID=35394736

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002562171A Abandoned CA2562171A1 (en) 2004-04-08 2005-04-08 Mutant .alpha.-amylases

Country Status (7)

Country Link
US (3) US20060014265A1 (https=)
EP (1) EP1733032A2 (https=)
JP (1) JP2007532117A (https=)
CN (1) CN1938422A (https=)
BR (1) BRPI0509671A (https=)
CA (1) CA2562171A1 (https=)
WO (1) WO2005111203A2 (https=)

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CN101052702B (zh) 2004-07-16 2013-01-09 杜邦营养生物科学有限公司 脂肪分解酶及其在食品工业中的应用
WO2007138049A2 (en) 2006-05-31 2007-12-06 Novozymes A/S Chloramphenicol resistance selection in bacillus licheniformis
US7968318B2 (en) 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
ES2584506T3 (es) * 2006-07-13 2016-09-28 Dsm Ip Assets B.V. Uso de amilasas bacterianas en pienso para animales bovinos
BRPI0722093A2 (pt) 2006-12-21 2014-04-01 Danisco Us Inc Genencor Div Composições e usos para um polipeptídeo de alfa-amilase da espécie bacillus 195
CN102559627B (zh) * 2007-01-25 2014-09-10 杜邦营养生物科学有限公司 由地衣芽孢杆菌转化细胞制备脂酰基转移酶
US20080220498A1 (en) * 2007-03-06 2008-09-11 Cervin Marguerite A Variant Buttiauxella sp. phytases having altered properties
ES2513217T3 (es) * 2007-02-07 2014-10-24 Danisco Us, Inc., Genencor Division Fitasas de Buttiauxella sp. variantes que tienen propiedades alteradas
CN101680005A (zh) 2007-03-14 2010-03-24 丹尼斯科美国公司 从大麦产生乙醇和含有降低的β-葡聚糖和肌醇六磷酸的DDGS
BRPI0809096A2 (pt) 2007-03-23 2014-09-09 Danisco Us Inc Genecor Division Produção aumentada de amilase através da adição n-terminal à proteína amilase madura
KR20100088675A (ko) 2007-11-05 2010-08-10 다니스코 유에스 인크. 변경된 특성을 지닌 바실러스 종 ts-23 알파-아밀라아제의 변이체
US8206966B2 (en) * 2007-11-05 2012-06-26 Danisco Us Inc. Alpha-amylase variants with altered properties
EP2222224B1 (en) * 2007-11-21 2017-06-28 Edda Technology, Inc. Method and system for interactive percutaneous pre-operation surgical planning
MX2010007014A (es) 2007-12-21 2010-09-30 Danisco Procedimiento para refinacion de aceite comestible utilizando una lipido aciltransferasa.
AU2009212526A1 (en) 2008-02-04 2009-08-13 Danisco Us Inc. TS23 alpha-amylase variants with altered properties
US8722381B2 (en) * 2008-02-04 2014-05-13 Danisco Us Inc. Variants of a Bacillus strearothermophilus alpha-amylase and uses thereof
US20110097778A1 (en) * 2008-04-30 2011-04-28 Power Scott D Chimeric alpha-amylase variants
CN102046783A (zh) 2008-06-06 2011-05-04 丹尼斯科美国公司 包含变体微生物蛋白酶的组合物和方法
MX2010013113A (es) * 2008-06-06 2010-12-21 Danisco Inc Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas.
CA2726631A1 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Saccharification enzyme composition and method of saccharification thereof
BRPI0913378A2 (pt) 2008-06-06 2015-09-01 Danisco Us Inc Produção de glicose a partir do amido usando alfa-amilase do bacillus subtilis
MX364987B (es) 2008-06-06 2019-05-17 Danisco Us Inc Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
WO2010036515A1 (en) 2008-09-25 2010-04-01 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
EP2406373B1 (en) 2009-03-10 2014-05-28 Danisco US Inc. Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof
EP2414515A2 (en) * 2009-04-01 2012-02-08 Danisco US Inc. Cleaning system comprising an alpha-amylase and a protease
WO2010117511A1 (en) 2009-04-08 2010-10-14 Danisco Us Inc. Halomonas strain wdg195-related alpha-amylases, and methods of use, thereof
EP2430169A2 (en) * 2009-05-12 2012-03-21 Danisco US Inc. Ethanol yields in fermentation from an improved liquefaction process
CA2771071C (en) 2009-08-07 2020-03-10 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
CA2778471A1 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
WO2011103549A1 (en) * 2010-02-22 2011-08-25 Syngenta Participations Ag Methods for distinguishing and identifying plant varieties
US20120276593A1 (en) 2011-04-29 2012-11-01 Danisco Us Inc. Use of cellulase and glucoamylase to improve ethanol yields from fermentation
EP2863827B1 (en) * 2012-06-21 2022-11-16 Globus Medical, Inc. Surgical robot platform
CA2893270C (en) 2012-12-11 2024-01-02 Danisco Us Inc. Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof
WO2015057517A1 (en) 2013-10-17 2015-04-23 Danisco Us Inc. Use of hemicellulases to improve ethanol production
WO2015193479A1 (en) * 2014-06-19 2015-12-23 KB Medical SA Systems and methods for performing minimally invasive surgery
US20180030456A1 (en) * 2015-02-19 2018-02-01 Danisco Us Inc. Enhanced protein expression
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
JP7025325B2 (ja) 2015-10-30 2022-02-24 ダニスコ・ユーエス・インク タンパク質発現の増強およびその方法
EP3421596A4 (en) * 2016-02-26 2019-10-23 Nanjing Bestzyme Bio-engineering Co. Ltd. ALPHA AMYLASE VERSION AND USE THEREOF
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
CN108823186B (zh) * 2018-07-03 2022-11-22 江西省科学院微生物研究所 一种玉米淀粉降解能力提高的嗜热酸性生淀粉α-淀粉酶突变体及其制备方法和应用
CN110607305A (zh) * 2019-08-30 2019-12-24 海南大学 斑马鱼α7乙酰胆碱受体重组载体及重组细胞
EP4165652A4 (en) 2020-06-15 2024-06-12 Bragg Analytics, Inc. DIFFRACTION-BASED GLOBAL IN VITRO DIAGNOSTIC SYSTEM
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
EP0208491B1 (en) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
AR000862A1 (es) * 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
ES2432519T3 (es) * 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
US6361989B1 (en) * 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US6187576B1 (en) * 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
US6309871B1 (en) * 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity
EP2184350B1 (en) * 1999-12-23 2014-09-03 Danisco US Inc. Cellulases having improved thermostability
AU3724801A (en) * 2000-03-03 2001-09-12 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
WO2002014490A2 (en) * 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries

Also Published As

Publication number Publication date
JP2007532117A (ja) 2007-11-15
WO2005111203A3 (en) 2006-03-30
US20080160573A1 (en) 2008-07-03
US20100261232A1 (en) 2010-10-14
BRPI0509671A (pt) 2007-10-30
EP1733032A2 (en) 2006-12-20
WO2005111203A2 (en) 2005-11-24
CN1938422A (zh) 2007-03-28
US20060014265A1 (en) 2006-01-19

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FZDE Dead

Effective date: 20130524