CA2548140A1 - Oligosaccharide compositions and use thereof in the treatment of infection - Google Patents
Oligosaccharide compositions and use thereof in the treatment of infection Download PDFInfo
- Publication number
- CA2548140A1 CA2548140A1 CA002548140A CA2548140A CA2548140A1 CA 2548140 A1 CA2548140 A1 CA 2548140A1 CA 002548140 A CA002548140 A CA 002548140A CA 2548140 A CA2548140 A CA 2548140A CA 2548140 A1 CA2548140 A1 CA 2548140A1
- Authority
- CA
- Canada
- Prior art keywords
- group
- composition
- lacto
- fucosyltransferase
- fucopentaose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000203 mixture Substances 0.000 title claims description 72
- 208000015181 infectious disease Diseases 0.000 title claims description 35
- 229920001542 oligosaccharide Polymers 0.000 title abstract description 250
- 150000002482 oligosaccharides Chemical class 0.000 title abstract description 248
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 112
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 68
- 238000000034 method Methods 0.000 claims abstract description 44
- 235000013336 milk Nutrition 0.000 claims description 94
- 210000004080 milk Anatomy 0.000 claims description 94
- 239000008267 milk Substances 0.000 claims description 93
- 235000020256 human milk Nutrition 0.000 claims description 71
- 210000004251 human milk Anatomy 0.000 claims description 70
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 67
- 235000018102 proteins Nutrition 0.000 claims description 66
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- SNFSYLYCDAVZGP-UHFFFAOYSA-N UNPD26986 Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(OC(O)C(O)C2O)CO)OC(CO)C(O)C1O SNFSYLYCDAVZGP-UHFFFAOYSA-N 0.000 claims description 42
- LQEBEXMHBLQMDB-JGQUBWHWSA-N GDP-beta-L-fucose Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C3=C(C(NC(N)=N3)=O)N=C2)O1 LQEBEXMHBLQMDB-JGQUBWHWSA-N 0.000 claims description 40
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- FZIVHOUANIQOMU-YIHIYSSUSA-N alpha-L-Fucp-(1->2)-beta-D-Galp-(1->3)-beta-D-GlcpNAc-(1->3)-beta-D-Galp-(1->4)-D-Glcp Chemical group O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@H]([C@H](O[C@@H]3[C@H]([C@H](O[C@@H]4[C@H](OC(O)[C@H](O)[C@H]4O)CO)O[C@H](CO)[C@@H]3O)O)O[C@H](CO)[C@H]2O)NC(C)=O)O[C@H](CO)[C@H](O)[C@@H]1O FZIVHOUANIQOMU-YIHIYSSUSA-N 0.000 claims description 35
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
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| US52759103P | 2003-12-05 | 2003-12-05 | |
| US60/527,591 | 2003-12-05 | ||
| PCT/US2004/040882 WO2005055944A2 (en) | 2003-12-05 | 2004-12-06 | Oligosaccharide compositions and use thereof in the treatment of infection |
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| CA002548140A Abandoned CA2548140A1 (en) | 2003-12-05 | 2004-12-06 | Oligosaccharide compositions and use thereof in the treatment of infection |
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| EP (2) | EP1689348B1 (enExample) |
| JP (1) | JP5236189B2 (enExample) |
| CA (1) | CA2548140A1 (enExample) |
| MX (1) | MXPA06006392A (enExample) |
| WO (1) | WO2005055944A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107108677A (zh) * | 2014-10-24 | 2017-08-29 | 格礼卡姆股份公司 | Hmo的混合物 |
Families Citing this family (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8124744B2 (en) * | 2006-05-01 | 2012-02-28 | Taisho Pharmaceutical Co., Ltd. | Macrolide derivatives |
| EP2134834B1 (en) * | 2007-03-07 | 2013-04-17 | GlycoFi, Inc. | Production of glycoproteins with modified fucosylation |
| US7871785B2 (en) | 2007-09-07 | 2011-01-18 | Children's Hospital Medical Center | Use of secretor, Lewis and sialyl antigen levels in clinical samples as predictors of risk for disease |
| EP2060257A1 (en) * | 2007-11-08 | 2009-05-20 | Nestec S.A. | Prevention and treatment of secondary infections following viral infection |
| EP2072052A1 (en) | 2007-12-17 | 2009-06-24 | Nestec S.A. | Prevention of opportunistic infections in immune-compromised subjects |
| WO2009155665A1 (en) * | 2008-06-26 | 2009-12-30 | Central Northern Adelaide Health Service | Methods and compositions for treating pathological infections |
| EP2331110B1 (en) * | 2008-09-25 | 2013-08-28 | Glycosyn LLC | Compositions and methods for engineering probiotic yeast |
| GB0817908D0 (en) * | 2008-10-01 | 2008-11-05 | Univ Ghent | Blood group a/b/h determinant on type 1 core glycosphinglipids chain as recognition point for the fedf protein of f18-fimbriated enterotoxigenic |
| KR101116868B1 (ko) * | 2008-10-20 | 2012-02-29 | 성균관대학교산학협력단 | 안질환의 예방 또는 치료용 조성물 |
| US20110300584A1 (en) * | 2008-12-19 | 2011-12-08 | Jennewein Biotechnologie Gmbh | Synthesis of fucosylated compounds |
| CA2757169A1 (en) * | 2009-04-13 | 2010-10-21 | Children's Hospital Medical Center | Milk oligosaccharide compositions and use thereof in treating infection in animals |
| EP2451462B1 (en) | 2009-07-06 | 2017-09-06 | Children's Hospital Medical Center | Inhibiting inflammation with milk oligosaccharides |
| ES2989649T3 (es) | 2009-07-15 | 2024-11-27 | Nutricia Nv | Mezcla de oligosacáridos no digeribles para estimular el sistema inmunitario |
| EP2813230A1 (en) * | 2009-07-15 | 2014-12-17 | N.V. Nutricia | Fucosyllactose as breast milk identical non-digestible oligosaccharide for treating and/or preventing infections |
| CN102946742A (zh) * | 2010-04-27 | 2013-02-27 | N·V·努特里奇亚 | 人乳寡糖在婴儿营养物中的用途 |
| WO2011136637A1 (en) * | 2010-04-27 | 2011-11-03 | N.V. Nutricia | Use of human milk oligosaccharides in infant nutrition |
| ES2680920T3 (es) | 2010-07-12 | 2018-09-11 | The Regents Of The University Of California | Oligosacáridos de leche bovina |
| WO2012031186A1 (en) | 2010-09-03 | 2012-03-08 | Wisconsin Pharmacal Company, Llc. | Prebiotic suppositories |
| EP2465509A1 (en) * | 2010-11-23 | 2012-06-20 | Nestec S.A. | Oligosaccharide composition for treating acute respiratory tract infections |
| EP2454948A1 (en) * | 2010-11-23 | 2012-05-23 | Nestec S.A. | Oligosaccharide mixture and food product comprising this mixture, especially infant formula |
| EP2455387A1 (en) * | 2010-11-23 | 2012-05-23 | Nestec S.A. | Oligosaccharide mixture and food product comprising this mixture, especially infant formula |
| EP2465507A1 (en) * | 2010-11-23 | 2012-06-20 | Nestec S.A. | Oligosaccharide composition for treating skin diseases |
| EP2465508A1 (en) * | 2010-11-23 | 2012-06-20 | Nestec S.A. | Composition comprising hydrolysed proteins and oligosaccharides for treating skin diseases |
| EP2658547A1 (en) | 2010-12-31 | 2013-11-06 | Abbott Laboratories | Methods for decreasing the incidence of necrotizing enterocolitis in infants, toddlers, or children using human milk oligosaccharides |
| MY166946A (en) | 2010-12-31 | 2018-07-25 | Abbott Lab | Methods of using human milk oligosaccharides for improving airway respiratory health |
| DK2658549T3 (da) | 2010-12-31 | 2020-06-29 | Abbott Lab | Fremgangsmåder til reduktion af incidensen af oxidativt stress under anvendelse af humane mælkeoligosaccharider, vitamin c og anti-inflammatoriske midler |
| SG191799A1 (en) | 2010-12-31 | 2013-08-30 | Abbott Lab | Nutritional formulations including human milk oligosaccharides and long chain polyunsaturated polyunsaturated fatty acids and uses thereof |
| US11337990B2 (en) | 2010-12-31 | 2022-05-24 | Abbott Laboratories | Human milk oligosaccharides to promote growth of beneficial bacteria |
| MX356994B (es) | 2010-12-31 | 2018-06-22 | Abbott Lab | Composiciones nutricionales que comprenden los oligosacaridos de leche humana y los nucleotidos y los usos de las mismas para tratar y/o prevenir la infeccion viral enterica. |
| EP3338784B1 (en) | 2010-12-31 | 2020-07-22 | Abbott Laboratories | Human milk oligosaccharides for modulating inflammation |
| MX2013008500A (es) | 2011-02-04 | 2014-03-05 | Univ California | Disialillacto-n-tetraosa (dslnt) o variantes, isomeros, analogos y derivados de la misma para prevenir o inhibir la enfermedad del intestino. |
| PH12013501501A1 (en) † | 2011-02-10 | 2019-10-07 | Nestec Sa | Modulation of growth of bifidobacteria using a combination of oligosaccharides found in human milk |
| US8795746B2 (en) * | 2011-02-16 | 2014-08-05 | The Board Of Trustees Of The Leland Stanford Junior University | Therapeutic use of mucin glycans |
| US20140187474A1 (en) * | 2011-02-16 | 2014-07-03 | The Board Of Trustees Of The Leland Stanford Junior University | Therapeutic use of mucin glycans |
| WO2012113404A1 (en) * | 2011-02-21 | 2012-08-30 | Glycom A/S | Novel glycosyl phosphites |
| CA2830025A1 (en) * | 2011-03-18 | 2012-09-27 | Glycom A/S | Synthesis of new fucose-containing carbohydrate derivatives |
| JP2014513985A (ja) * | 2011-05-13 | 2014-06-19 | グリコム アー／エス | 人乳オリゴ糖（ｈｍｏ）又はその前駆体の形成方法 |
| RU2013155453A (ru) | 2011-05-13 | 2015-06-20 | Глюком А/С | Диверсификация молочных олигосахаридов человека (hmos) или их предшественников |
| JP6129821B2 (ja) * | 2011-05-13 | 2017-05-17 | グリコシン リミテッド ライアビリティー カンパニー | プレバイオティクスとしての、精製された２’−フコシルラクトース、３−フコシルラクトース、およびラクトジフコテトラオースの使用 |
| ES2671555T3 (es) | 2011-06-07 | 2018-06-07 | Hero Ag | Obtención de oligosacáridos mediante un proceso biotecnológico |
| NL2007268C2 (en) | 2011-08-16 | 2013-02-19 | Friesland Brands Bv | Nutritional compositions comprising human milk oligosaccharides and uses thereof. |
| CA2846603A1 (en) | 2011-08-29 | 2013-03-07 | Abbott Laboratories | Human milk oligosaccharides for preventing injury and/or promoting healing of the gastrointestinal tract |
| NL2007931C2 (en) * | 2011-12-07 | 2013-06-10 | Friesland Brands Bv | Methods for providing sialylated oligosaccharides and products obtainable thereby. |
| US9655912B2 (en) | 2012-02-16 | 2017-05-23 | Akeso Biomedical, Inc. | Reduction of gastrointestinal tract colonisation by campylobacter |
| EP2656862A1 (en) * | 2012-04-24 | 2013-10-30 | The Procter & Gamble Company | Substrate comprising one or more human milk oligosaccharides and disposable absorbent article comprising the substrate |
| US9695454B2 (en) | 2012-05-23 | 2017-07-04 | Glykos Finland Oy | Production of fucosylated glycoproteins |
| US9770419B2 (en) * | 2012-08-01 | 2017-09-26 | Shaker A. Mousa | Methods and compositions of camel derived products |
| TWI567200B (zh) * | 2012-08-20 | 2017-01-21 | 中央研究院 | 寡醣之大規模酵素合成 |
| ES2640978T3 (es) | 2012-10-31 | 2017-11-07 | Jennewein Biotechnologie Gmbh | Proceso de producción de monosacáridos |
| WO2014069625A1 (ja) * | 2012-11-01 | 2014-05-08 | 協和発酵バイオ株式会社 | オリゴ糖の結晶およびその製造方法 |
| EP2769726B1 (en) * | 2013-02-21 | 2018-10-31 | Jennewein Biotechnologie GmbH | Synthetic or recombinant fucosylated Oligosaccarides for use in the treatment of infections |
| WO2014130789A1 (en) | 2013-02-21 | 2014-08-28 | Children's Hospital Medical Center | Use of glycans and glycosyltransferases for diagnosing/monitoring inflammatory bowel disease |
| DK2999358T3 (en) | 2013-05-22 | 2021-10-11 | Glycom As | Synthetic mixture of oligosaccharides for the treating a microbiota of a mammal |
| US9321803B2 (en) | 2013-07-12 | 2016-04-26 | Children's Hospital Medical Center | Compositions and methods for inhibiting norovirus infection |
| AU2014350144B2 (en) | 2013-11-15 | 2019-11-21 | Société des Produits Nestlé S.A. | Compositions for use in the prevention or treatment of URT infections in infants or young children at risk |
| US10568896B2 (en) | 2013-11-19 | 2020-02-25 | Abbott Laboratories | Methods for preventing or mitigating acute allergic responses using human milk oligosaccharides |
| HK1231398A1 (zh) | 2014-04-08 | 2017-12-22 | Abbott Laboratories | 使用人乳寡糖增强对病原体的粘膜先天免疫反应和/或检测的方法 |
| CN108064266A (zh) | 2014-07-21 | 2018-05-22 | 格利科斯芬兰公司 | 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备 |
| CA2957790A1 (en) | 2014-08-13 | 2016-02-18 | Akeso Biomedical, Inc. | Antimicrobial compounds and compositions, and uses thereof |
| CA2960835A1 (en) * | 2014-09-09 | 2016-03-17 | Glycosyn LLC | Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides |
| FI3264923T3 (fi) | 2015-03-05 | 2023-04-21 | Nestle Sa | Koostumuksia käytettäväksi ruoansulatuskanavan infektioiden ehkäisemisessä tai hoitamisessa imeväisillä tai nuorilla lapsilla |
| CN107427054A (zh) | 2015-03-05 | 2017-12-01 | 雀巢产品技术援助有限公司 | 用于预防或治疗婴儿或幼儿的耳炎或支气管炎的组合物 |
| US10821124B2 (en) * | 2015-03-05 | 2020-11-03 | Societe Des Produits Nestle S.A. | Compositions for use in improving stool consistency or frequency in infants or young children |
| US10857167B2 (en) | 2015-04-28 | 2020-12-08 | Children's Hospital Medical Center | Use of oligosaccharide compositions to enhance weight gain |
| WO2016205332A1 (en) * | 2015-06-15 | 2016-12-22 | Zuchem, Inc. | Activated n-acetylated sugars and oligosaccharides |
| ITUB20152818A1 (it) * | 2015-08-03 | 2017-02-03 | Consorzio Univ Unifarm | Formulazioni oftalmiche a base di oligosaccaridi |
| US10653658B2 (en) | 2015-08-11 | 2020-05-19 | Akeso Biomedical, Inc. | Biofilm inhibiting compositions enhancing weight gain in livestock |
| CN108024563A (zh) | 2015-08-11 | 2018-05-11 | 艾索生物医药公司 | 提高家畜中重量增加的生物膜抑制组合物 |
| RU2720243C2 (ru) * | 2015-09-02 | 2020-04-28 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Глюкозидгидролазы и их применение в предупреждении и/или лечении патогенной инфекции у животного |
| WO2017083520A1 (en) * | 2015-11-13 | 2017-05-18 | Cadena Bio, Inc. | Animal therapeutic and feed compositions and methods of use |
| WO2017129648A1 (en) | 2016-01-26 | 2017-08-03 | Nestec S.A. | Human milk oligosaccharides for health benefits by prevention of premature adiposity rebound |
| EP3493817A1 (en) * | 2016-08-04 | 2019-06-12 | Nestec S.A. | Nutritional compositions with 2fl and lnnt for use in preventing and/or treating non-rotavirus diarrhea by acting on the gut microbiota dysbiosis |
| US20180103675A1 (en) * | 2016-10-14 | 2018-04-19 | Mead Johnson Nutrition Company | Personalized pediatric nutrition products comprising human milk oligosaccharides |
| CA3053522A1 (en) | 2017-03-28 | 2018-10-04 | Children's Hospital Medical Center | Norovirus s particle based vaccines and methods of making and using same |
| EP3425052A1 (en) * | 2017-07-07 | 2019-01-09 | Jennewein Biotechnologie GmbH | Fucosyltransferases and their use in producing fucosylated oligosaccharides |
| WO2019031961A1 (en) | 2017-08-11 | 2019-02-14 | N.V. Nutricia | OLIGOSACCHARIDE OF HUMAN MILK TO IMPROVE PHYSICAL IMMUNE CONDITION |
| RS20171030A1 (sr) | 2017-10-12 | 2019-04-30 | Pavlovic Bojan | F-fukoidani, desulfonovani f-fukoidani i njihovi derivati, desulfonovane oligo-fukoze kao inhibitori gastrointestinalnih infekcija |
| EP3716983A4 (en) * | 2017-11-30 | 2022-03-02 | Glycom A/S | HUMAN MILK OLIGOSACCHARIDES AND SYNTHETIC COMPOSITIONS THEREOF FOR MICROBIOTA MODULATION |
| US10519475B1 (en) * | 2018-11-21 | 2019-12-31 | Amyris, Inc. | Biosynthesis of compounds in yeast |
| EP3935581A4 (en) | 2019-03-04 | 2022-11-30 | Iocurrents, Inc. | DATA COMPRESSION AND COMMUNICATION USING MACHINE LEARNING |
| AU2021273198A1 (en) | 2020-05-13 | 2023-02-02 | Glycosyn LLC | Fucosylated oligosaccharides for prevention of coronavirus infection |
| AU2021271697A1 (en) | 2020-05-13 | 2023-02-02 | Glycosyn LLC | 2'-fucosyllactose for the prevention and treatment of coronavirus-induced inflammation |
| US10947552B1 (en) | 2020-09-30 | 2021-03-16 | Alpine Roads, Inc. | Recombinant fusion proteins for producing milk proteins in plants |
| WO2022072718A1 (en) | 2020-09-30 | 2022-04-07 | Nobell Foods, Inc. | Recombinant milk proteins and food compositions comprising the same |
| US10894812B1 (en) | 2020-09-30 | 2021-01-19 | Alpine Roads, Inc. | Recombinant milk proteins |
| WO2023110994A1 (en) * | 2021-12-14 | 2023-06-22 | Inbiose N.V. | Production of alpha-1,4-fucosylated compounds |
| US20250297294A1 (en) * | 2021-12-23 | 2025-09-25 | Amyris, Inc. | Compositions and methods for improved production of human milk oligosaccharides |
| WO2024042235A1 (en) * | 2022-08-25 | 2024-02-29 | Dsm Ip Assets B.V. | Hybrid method for producing complex hmos |
| WO2025111563A1 (en) * | 2023-11-22 | 2025-05-30 | Matrubials Inc. | Milk peptide compositions for pharmaceutical and cosmeceutical use |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340772C (en) | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
| DE68925893T2 (de) | 1988-07-23 | 1996-08-08 | Delta Biotechnology Ltd | Sekretorische leader-sequenzen |
| DK455088D0 (da) * | 1988-08-12 | 1988-08-12 | Symbicom Ab | Syntetiske receptoranaloger |
| US5874261A (en) | 1988-09-02 | 1999-02-23 | The Trustees Of The University Of Pennsylvania | Method for the purification of glycosyltransferases |
| US6306625B1 (en) | 1988-12-30 | 2001-10-23 | Smithkline Beecham Biologicals, Sa | Method for obtaining expression of mixed polypeptide particles in yeast |
| US6172039B1 (en) | 1990-04-16 | 2001-01-09 | Apex Bioscience, Inc. | Expression of recombinant hemoglobin and hemoglobin variants in yeast |
| WO1992005244A1 (en) | 1990-09-13 | 1992-04-02 | Duke University | Expression of g protein coupled receptors in yeast |
| DK0673427T3 (da) | 1992-07-08 | 2003-07-21 | Unilever Nv | Enzymatisk proces under anvendelse af enzymer immobiliseret på cellevæggen af en eukaryotisk mikrobiel celle ved fremstilling af et fusionsprotein |
| EP0656940B1 (en) | 1992-07-30 | 2003-10-01 | INTERNATIONAL FLOWER DEVELOPMENTS Pty. Ltd. | Genetic sequences encoding glycosyltransferase enzymes and uses therefor |
| US5874271A (en) | 1992-08-21 | 1999-02-23 | Takara Shuzo Co., Ltd. | Human glycosyltransferase gene, compounds and method for inhibiting cancerous metastasis |
| EP0601417A3 (de) * | 1992-12-11 | 1998-07-01 | Hoechst Aktiengesellschaft | Physiologisch verträglicher und physiologisch abbaubarer, Kohlenhydratrezeptorblocker auf Polymerbasis, ein Verfahren zu seiner Herstellung und seine Verwendung |
| JP3691055B2 (ja) | 1993-02-10 | 2005-08-31 | ユニリーバー・ナームローゼ・ベンノートシャープ | 特異的結合力をもつ固定化タンパク質並びに各種プロセス・物品におけるそれらの使用 |
| US5876951A (en) | 1993-03-31 | 1999-03-02 | Cadus Pharmaceutical Corporation | Yeast cells engineered to produce pheromone system protein surrogates and uses therefor |
| US6100042A (en) | 1993-03-31 | 2000-08-08 | Cadus Pharmaceutical Corporation | Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor |
| US5484773A (en) * | 1994-02-14 | 1996-01-16 | Alberta Research Council | Treatment of antibiotic associated diarrhea |
| FI963197A7 (fi) | 1994-02-16 | 1996-08-15 | Pharming Intellectual Property Bv | Laktoferriinin eristäminen maidosta |
| US6204431B1 (en) | 1994-03-09 | 2001-03-20 | Abbott Laboratories | Transgenic non-human mammals expressing heterologous glycosyltransferase DNA sequences produce oligosaccharides and glycoproteins in their milk |
| NZ281041A (en) * | 1994-03-09 | 1998-04-27 | Abbott Lab | Non-human mammals transformed with a catalytic entity of enzymes or antibodies to produce a heterologous product in the animals milk |
| US5750176A (en) * | 1994-03-09 | 1998-05-12 | Abbott Laboratories | Transgenic non-human mammal milk comprising 2'-fucosyl-lactose |
| USRE37206E1 (en) | 1994-06-13 | 2001-06-05 | Banyu Pharmaceutical Co, Ltd | Gene encoding glycosyltransferase and its uses |
| US5576300A (en) * | 1994-09-16 | 1996-11-19 | Abbott Laboratories | Method for inhibition of human rotavirus infection |
| US5545553A (en) | 1994-09-26 | 1996-08-13 | The Rockefeller University | Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them |
| US5858752A (en) | 1995-06-07 | 1999-01-12 | The General Hospital Corporation | Fucosyltransferase genes and uses thereof |
| JP2758856B2 (ja) | 1995-06-15 | 1998-05-28 | 理化学研究所 | セラミドグルコシルトランスフェラーゼ |
| WO1997027303A1 (en) | 1996-01-24 | 1997-07-31 | Toyo Boseki Kabushiki Kaisha | Alpha-1-6 fucosyltransferases |
| US5856159A (en) | 1996-03-27 | 1999-01-05 | Cytel Corporation | Production of galactosyltransferase |
| US6300065B1 (en) | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US5821098A (en) | 1996-09-13 | 1998-10-13 | Eli Lilly And Company | Glycosyltransferase gene gtfD from amycolatopsis orientalis |
| US5821100A (en) | 1996-09-13 | 1998-10-13 | Eli Lilly And Company | Glycosyltransferase gene gtfb from Amycolatopsis orientalis |
| US5871983A (en) | 1996-09-13 | 1999-02-16 | Eli Lilly And Company | Glucosyltransferase gene GTFE from amycolatopsis orientalis |
| US5821097A (en) | 1996-09-13 | 1998-10-13 | Eli Lilly And Company | Glycosyltransferase gene GtfC from Amycolatopsis orientalis |
| EP0870841A4 (en) * | 1996-09-17 | 2001-04-11 | Kyowa Hakko Kogyo Kk | PROCESSES FOR THE PRODUCTION OF SUGAR NUCLEOTIDES AND COMPLEX CARBOHYDRATES |
| WO1998013513A2 (en) | 1996-09-24 | 1998-04-02 | Cadus Pharmaceutical Corporation | Methods and compositions for identifying receptor effectors |
| EP0948538B1 (en) | 1996-12-13 | 2008-06-11 | Novartis Vaccines and Diagnostics, Inc. | Analysis and separation of platelet-derived growth factor proteins |
| US6033883A (en) | 1996-12-18 | 2000-03-07 | Kosan Biosciences, Inc. | Production of polyketides in bacteria and yeast |
| US5922540A (en) | 1996-12-20 | 1999-07-13 | Eli Lilly And Company | Monofunctional glycosyltransferase gene of Staphylococcus aureus |
| AUPO468597A0 (en) | 1997-01-21 | 1997-02-13 | Montech Medical Developments Pty Ltd | Expression in yeast of antigenically active, recombinant hybrid glutamic acid decarboxylase |
| AU729158B2 (en) | 1997-01-31 | 2001-01-25 | Genentech Inc. | O-fucosyltransferase |
| US6045854A (en) * | 1997-03-31 | 2000-04-04 | Abbott Laboraties | Nutritional formulations containing oligosaccharides |
| US6087143A (en) | 1997-09-05 | 2000-07-11 | Eli Lilly And Company | Glycosyltransferase gene gtfA from Amycolatopsis orientalis |
| US7244601B2 (en) * | 1997-12-15 | 2007-07-17 | National Research Council Of Canada | Fusion proteins for use in enzymatic synthesis of oligosaccharides |
| FR2774393B1 (fr) | 1998-02-05 | 2000-03-24 | Hoechst Marion Roussel Inc | Souches de levure ayant le gene atf2 interrompu et leurs applications |
| US5955282A (en) | 1998-04-03 | 1999-09-21 | Incyte Pharmaceuticals, Inc. | Human galactosyltransferases |
| US6126961A (en) * | 1998-04-13 | 2000-10-03 | Kross; Robert D. | Composition and method for reducing the colonization of animal intestines by salmonella and other bacterial pathogens |
| US20020019991A1 (en) * | 1998-04-30 | 2002-02-14 | Abbott Laboratories | Compositions containing an alpha 1,2-fucose linkage and uses thereof |
| US6238894B1 (en) | 1998-11-04 | 2001-05-29 | Diane Taylor | α1,2 fucosyltransferase |
| US6291435B1 (en) * | 1999-03-04 | 2001-09-18 | The Governs Of The University Of Alberta | Treatment of diarrhea caused by enteropathogenic Escherichia coli |
| FI109811B (fi) * | 2000-09-26 | 2002-10-15 | Medicel Oy | Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä |
| JP2002320485A (ja) | 2000-11-21 | 2002-11-05 | National Institute Of Advanced Industrial & Technology | 融合遺伝子発現ベクター及び固定化酵素の製造方法 |
-
2004
- 2004-12-06 EP EP04813227.8A patent/EP1689348B1/en not_active Expired - Lifetime
- 2004-12-06 CA CA002548140A patent/CA2548140A1/en not_active Abandoned
- 2004-12-06 EP EP12169947A patent/EP2497478A3/en not_active Withdrawn
- 2004-12-06 JP JP2006542875A patent/JP5236189B2/ja not_active Expired - Fee Related
- 2004-12-06 US US10/581,759 patent/US7893041B2/en not_active Expired - Fee Related
- 2004-12-06 MX MXPA06006392A patent/MXPA06006392A/es active IP Right Grant
- 2004-12-06 WO PCT/US2004/040882 patent/WO2005055944A2/en not_active Ceased
-
2011
- 2011-02-22 US US13/032,568 patent/US20110207659A1/en not_active Abandoned
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107108677A (zh) * | 2014-10-24 | 2017-08-29 | 格礼卡姆股份公司 | Hmo的混合物 |
| CN107108677B (zh) * | 2014-10-24 | 2020-08-07 | 格礼卡姆股份公司 | Hmo的混合物 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005055944A9 (en) | 2005-07-14 |
| US7893041B2 (en) | 2011-02-22 |
| EP2497478A2 (en) | 2012-09-12 |
| MXPA06006392A (es) | 2007-03-15 |
| EP2497478A3 (en) | 2012-11-07 |
| WO2005055944A3 (en) | 2007-06-28 |
| US20110207659A1 (en) | 2011-08-25 |
| WO2005055944A2 (en) | 2005-06-23 |
| EP1689348A2 (en) | 2006-08-16 |
| EP1689348B1 (en) | 2013-05-15 |
| US20070275881A1 (en) | 2007-11-29 |
| EP1689348A4 (en) | 2008-11-26 |
| JP2007525487A (ja) | 2007-09-06 |
| JP5236189B2 (ja) | 2013-07-17 |
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