CA2542192C - Look-through mutagenesis - Google Patents
Look-through mutagenesis Download PDFInfo
- Publication number
- CA2542192C CA2542192C CA2542192A CA2542192A CA2542192C CA 2542192 C CA2542192 C CA 2542192C CA 2542192 A CA2542192 A CA 2542192A CA 2542192 A CA2542192 A CA 2542192A CA 2542192 C CA2542192 C CA 2542192C
- Authority
- CA
- Canada
- Prior art keywords
- ser
- amino acid
- thr
- polypeptide
- gly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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| US20110129414A1 (en) * | 2005-11-15 | 2011-06-02 | Balyasnikova Irina V | Single Chain Fragment of Monoclonal Antibody 9B9 and Uses Thereof |
| JP2009519983A (ja) | 2005-12-20 | 2009-05-21 | アラーナ・テラピューティクス・リミテッド | 部分的な新世界ザル結合領域を有するキメラ抗体 |
| EA017417B1 (ru) | 2006-02-01 | 2012-12-28 | Сефалон Астралия Пти Лтд. | КОНСТРУКТ ОДНОДОМЕННОГО АНТИТЕЛА, КОТОРЫЙ СВЯЗЫВАЕТСЯ С ЧЕЛОВЕЧЕСКИМ TNF-α, И ЕГО ПРИМЕНЕНИЕ |
| US7704953B2 (en) * | 2006-02-17 | 2010-04-27 | Mdrna, Inc. | Phage displayed cell binding peptides |
| WO2007112054A2 (en) * | 2006-03-23 | 2007-10-04 | Sea Lane Biotechnologies, Llc | Facilitation of translocation of molecules through the gastrointestinal tract |
| WO2007134327A2 (en) | 2006-05-15 | 2007-11-22 | Sea Lane Biotechnologies, Llc. | Neutralizing antibodies to influenza viruses |
| WO2007136840A2 (en) * | 2006-05-20 | 2007-11-29 | Codon Devices, Inc. | Nucleic acid library design and assembly |
| EP2057191A1 (en) | 2006-08-18 | 2009-05-13 | Ablynx N.V. | Amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of deseases and disorders associated with il-6-mediated signalling |
| US8131480B2 (en) | 2006-10-02 | 2012-03-06 | Sea Lane Biotechnologies Llc | Construction of diverse synthetic peptide and polypeptide libraries |
| US20080287320A1 (en) * | 2006-10-04 | 2008-11-20 | Codon Devices | Libraries and their design and assembly |
| JP5594895B2 (ja) * | 2007-06-06 | 2014-09-24 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | タンパク質の能力の改良方法 |
| WO2009009045A2 (en) * | 2007-07-10 | 2009-01-15 | The Scripps Research Institute | Escape libraries of target polypeptides |
| US8680019B2 (en) * | 2007-08-10 | 2014-03-25 | Protelica, Inc. | Universal fibronectin Type III binding-domain libraries |
| US8470966B2 (en) | 2007-08-10 | 2013-06-25 | Protelica, Inc. | Universal fibronectin type III binding-domain libraries |
| US20090176654A1 (en) * | 2007-08-10 | 2009-07-09 | Protelix, Inc. | Universal fibronectin type III binding-domain libraries |
| CN101970490A (zh) | 2007-11-27 | 2011-02-09 | 埃博灵克斯股份有限公司 | 针对异二聚体细胞因子和/或其受体的氨基酸序列以及包括所述氨基酸序列的多肽 |
| EP2698380A1 (en) | 2008-03-28 | 2014-02-19 | Sea Lane Biotechnologies, LLC | Neutralizing molecules to viral antigens |
| EP2131245A3 (en) * | 2008-06-02 | 2012-08-01 | ASML Netherlands BV | Lithographic apparatus and its focus determination method |
| WO2010115995A2 (en) | 2009-04-10 | 2010-10-14 | Ablynx Nv | Improved amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorders |
| EP2417163B1 (en) | 2009-04-10 | 2019-02-27 | Ablynx N.V. | Improved amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorders |
| MX340541B (es) | 2009-06-05 | 2016-07-13 | Alblynx Nv | Secuencias de aminoacidos mejoradas dirigidas contra virus sincitial respiratorio humano y polipeptidos que comprenden las mismas para la prevencion y/o tratamiento de infecciones del tracto respiratorio. |
| CN102625848A (zh) * | 2009-07-17 | 2012-08-01 | 生物蛋白有限公司 | 在生产宿主中同时整合抗体/蛋白的性能和表达的筛选和演化的方法 |
| GB0914691D0 (en) * | 2009-08-21 | 2009-09-30 | Lonza Biologics Plc | Immunoglobulin variants |
| ES2860453T3 (es) | 2009-10-30 | 2021-10-05 | Novartis Ag | Bibliotecas universales del dominio de unión del lado inferior de la fibronectina de tipo III |
| US9644022B2 (en) | 2009-11-30 | 2017-05-09 | Ablynx N.V. | Amino acid sequences directed against human respiratory syncytial virus (HRSV) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections |
| AU2011283646B2 (en) | 2010-07-30 | 2015-07-09 | Novartis Ag | Fibronectin cradle molecules and libraries thereof |
| US9753040B2 (en) | 2010-12-01 | 2017-09-05 | Mitsubishi Tanabe Pharma Corporation | Polynucleotide construct capable of displaying fab in a cell-free translation system, and method for manufacturing and screening fab using same |
| WO2013041722A1 (en) | 2011-09-23 | 2013-03-28 | Ablynx Nv | Prolonged inhibition of interleukin-6 mediated signaling |
| JP6415987B2 (ja) | 2012-03-02 | 2018-10-31 | アブリンクス エン．ヴェー． | シュードモナス・エルギノーサのＰｃｒＶに結合する単一可変ドメイン抗体 |
| WO2013158217A1 (en) * | 2012-04-20 | 2013-10-24 | Thomas Jefferson University | Engineered antibody for inhibition of fibrosis |
| DK2855674T4 (da) | 2012-05-25 | 2022-11-21 | Chr Hansen As | Chymosinvarianter med forbedrede mælkekoagulerende egenskaber |
| WO2014087010A1 (en) | 2012-12-07 | 2014-06-12 | Ablynx N.V. | IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE |
| KR101600899B1 (ko) * | 2012-12-11 | 2016-03-09 | 주식회사 셀레믹스 | 초병렬적 핵산 합성법을 통한 dna 라이브러리의 동시 합성 방법 |
| US20140314741A1 (en) * | 2013-04-18 | 2014-10-23 | Developmen Center For Biotechnology | Human Antibody against Interleukin-20 and Treatment for Inflammatory Diseases |
| WO2015065987A1 (en) * | 2013-11-01 | 2015-05-07 | Ibc Pharmaceuticals, Inc. | Bispecific antibodies that neutralize both tnf-alpha and il-6: novel therapeutic agent for autoimmune disease |
| CN104805507B (zh) * | 2014-01-29 | 2019-01-22 | 杭州康万达医药科技有限公司 | 噬菌体展示文库及其应用和制备方法 |
| BR112016017502B1 (pt) | 2014-02-26 | 2023-10-24 | Chr. Hansen A/S | Variantes de polipeptídeo de quimosina isoladas método para a produção das mesmas e método para a fabricação de um produto de alimentação humana ou animal |
| US10118967B2 (en) | 2014-10-21 | 2018-11-06 | Ablynx N.V. | Methods for treating rheumatoid arthritis by administering IL-6 receptor antibodies |
| CN107849550B (zh) | 2015-06-22 | 2021-09-03 | 科.汉森有限公司 | 具有改善的性质的凝乳酶变体 |
| JP2018525996A (ja) | 2015-08-31 | 2018-09-13 | セーホーエル．ハンセン アクティーゼルスカブ | 改良された特性を有するキモシンの変異体 |
| WO2017119499A1 (en) * | 2016-01-08 | 2017-07-13 | Momotaro-Gene Inc. | A combination therapy using REIC/Dkk-3 gene and a checkpoint inhibitor |
| CN109640677A (zh) | 2016-05-19 | 2019-04-16 | 科·汉森有限公司 | 具有改善的凝乳特性的凝乳酶的变体 |
| JP7395251B2 (ja) | 2016-05-19 | 2023-12-11 | セーホーエル．ハンセン アクティーゼルスカブ | 改善された凝乳特性を有するキモシン変異体 |
| CN117298260A (zh) * | 2016-09-02 | 2023-12-29 | 莱蒂恩技术公司 | 用DuoCAR治疗癌症的组合物和方法 |
| MX2020006297A (es) | 2017-12-15 | 2020-12-07 | Aleta Biotherapeutics Inc | Variantes de cd19. |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0527809B1 (en) * | 1990-04-05 | 1995-08-16 | CREA, Roberto | Walk-through mutagenesis |
| WO1995023813A1 (en) * | 1994-03-04 | 1995-09-08 | Merck & Co., Inc. | In vitro antibody affinity maturation using alanine scanning mutagenesis |
| US20030180714A1 (en) * | 1999-12-15 | 2003-09-25 | Genentech, Inc. | Shotgun scanning |
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2004
- 2004-06-25 KR KR1020057025136A patent/KR20060034650A/ko not_active Withdrawn
- 2004-06-25 CA CA2542192A patent/CA2542192C/en not_active Expired - Lifetime
- 2004-06-25 CN CNA2004800229706A patent/CN1836041A/zh active Pending
- 2004-06-25 AU AU2004254352A patent/AU2004254352A1/en not_active Abandoned
- 2004-06-25 ES ES04756045.3T patent/ES2609102T3/es not_active Expired - Lifetime
- 2004-06-25 BR BRPI0412007-8A patent/BRPI0412007A/pt not_active IP Right Cessation
- 2004-06-25 EP EP04756045.3A patent/EP1660655B1/en not_active Expired - Lifetime
- 2004-06-25 US US10/877,467 patent/US20050136428A1/en not_active Abandoned
- 2004-06-25 RU RU2005140664/13A patent/RU2005140664A/ru not_active Application Discontinuation
- 2004-06-25 JP JP2006517625A patent/JP4791960B2/ja not_active Expired - Lifetime
- 2004-06-25 WO PCT/US2004/020306 patent/WO2005003345A2/en not_active Ceased
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| JP5358604B2 (ja) | 2013-12-04 |
| IL172736A0 (en) | 2006-04-10 |
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| EP1660655B1 (en) | 2016-11-02 |
| EP1660655A2 (en) | 2006-05-31 |
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| KR20060034650A (ko) | 2006-04-24 |
| JP2007524390A (ja) | 2007-08-30 |
| AU2004254352A1 (en) | 2005-01-13 |
| ZA200510460B (en) | 2006-11-29 |
| WO2005003345A3 (en) | 2005-03-24 |
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| WO2005003345A2 (en) | 2005-01-13 |
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| ES2609102T3 (es) | 2017-04-18 |
| US20050136428A1 (en) | 2005-06-23 |
| CA2542192A1 (en) | 2005-01-13 |
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