CA2539457C - Nucleic acid and gene derived from novel hcv strain and replicon-replicating cell using said gene - Google Patents

Nucleic acid and gene derived from novel hcv strain and replicon-replicating cell using said gene Download PDF

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Publication number
CA2539457C
CA2539457C CA2539457A CA2539457A CA2539457C CA 2539457 C CA2539457 C CA 2539457C CA 2539457 A CA2539457 A CA 2539457A CA 2539457 A CA2539457 A CA 2539457A CA 2539457 C CA2539457 C CA 2539457C
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CA
Canada
Prior art keywords
replicon
rna
cells
replicon rna
hcv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2539457A
Other languages
English (en)
French (fr)
Other versions
CA2539457A1 (en
Inventor
Takaji Wakita
Takanobu Kato
Tomoko Date
Michiko Miyamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tokyo Metropolitan Institute of Medical Science
Toray Industries Inc
Original Assignee
Tokyo Metropolitan Institute of Medical Science
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tokyo Metropolitan Institute of Medical Science, Toray Industries Inc filed Critical Tokyo Metropolitan Institute of Medical Science
Publication of CA2539457A1 publication Critical patent/CA2539457A1/en
Application granted granted Critical
Publication of CA2539457C publication Critical patent/CA2539457C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24221—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
CA2539457A 2003-09-19 2004-09-16 Nucleic acid and gene derived from novel hcv strain and replicon-replicating cell using said gene Expired - Fee Related CA2539457C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2003329082 2003-09-19
JP2003-329082 2003-09-19
PCT/JP2004/014003 WO2005028652A1 (ja) 2003-09-19 2004-09-16 新規hcv株由来の核酸、遺伝子、及び該遺伝子を利用したレプリコン複製細胞

Publications (2)

Publication Number Publication Date
CA2539457A1 CA2539457A1 (en) 2005-03-31
CA2539457C true CA2539457C (en) 2013-12-03

Family

ID=34372954

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2539457A Expired - Fee Related CA2539457C (en) 2003-09-19 2004-09-16 Nucleic acid and gene derived from novel hcv strain and replicon-replicating cell using said gene

Country Status (6)

Country Link
US (1) US8022197B2 (ja)
EP (1) EP1666598B1 (ja)
JP (1) JP4573776B2 (ja)
CN (1) CN1882690B (ja)
CA (1) CA2539457C (ja)
WO (1) WO2005028652A1 (ja)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7790448B2 (en) * 2003-09-19 2010-09-07 Tokyo Metropolitan Organization For Medical Research Nucleic acid and gene derived from novel HCV strain and replicon-replicating cell using said gene
JP5086082B2 (ja) * 2004-10-01 2012-11-28 ノバルティス ヴァクシンズ アンド ダイアグノスティクス エスアールエル Ｃ型肝炎ウイルス複製系
WO2007011777A2 (en) * 2005-07-18 2007-01-25 Novartis Ag Small animal model for hcv replication
WO2010074249A1 (ja) 2008-12-26 2010-07-01 東レ株式会社 Ｃ型肝炎ウイルス由来の核酸並びにそれを用いた発現ベクター、形質転換細胞及びｃ型肝炎ウイルス粒子
EP2471917A4 (en) 2009-08-28 2013-03-13 Tokyo Metropolitan Inst Medical Science POLYNUCLEOTIDE FROM A NEW HEPATITIS C VIRUS STRAIN AND USE THEREOF
US9234184B2 (en) 2011-03-31 2016-01-12 Japan As Represented By Director-General Of National Institute Of Infectious Diseases Nucleic acid construct comprising nucleic acid derived from genome of hepatitis C virus of genotype 1B, hepatitis C virus genome-replicating cells transfected with the same, and method for producing infectious hepatitis C virus particles
EP2752485A4 (en) * 2011-08-31 2015-06-24 Japan As Represented By The Director General Of Nat Inst Of Infectious Diseases NUCLEIC ACID RECOMBINANT PRODUCT COMPRISING NUCLEIC ACID DERIVED FROM GENOTYPE 3A GENE VHC GENOME

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428145A (en) * 1991-08-09 1995-06-27 Immuno Japan, Inc. Non-A, non-B, hepatitis virus genome, polynucleotides, polypeptides, antigen, antibody and detection systems
JPH06121689A (ja) * 1991-08-09 1994-05-06 Tetsuo Nakamura 非ａ非ｂ型肝炎ウイルス遺伝子、ポリヌクレオチド、ポリペ プタイド、抗原、抗体検出系
DE19915178A1 (de) * 1999-04-03 2000-10-05 Univ Mainz Johannes Gutenberg Hepatitis C Virus Zellkultursystem
EP1185665B1 (en) 1999-06-04 2008-09-10 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services INFECTIOUS cDNA CLONE OF GB VIRUS B AND USES THEREOF
DE60034884T2 (de) 1999-06-04 2008-01-17 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Klonierte genome von infektiösen hepatitis c viren genotyp 2a und deren verwendungen
JP4880116B2 (ja) 2000-12-01 2012-02-22 財団法人 東京都医学総合研究所 劇症ｃ型肝炎ウイルス株の遺伝子
US20030009775A1 (en) * 2001-05-25 2003-01-09 Glenn Jeffrey S. Mouse model for hepatitis C
WO2003085084A2 (en) * 2002-04-03 2003-10-16 Smithkline Beecham Corporation Hepatitis c virus sub-genomic replicons
US7455969B2 (en) 2002-11-13 2008-11-25 Washington University Highly permissive cell lines for hepatitis C virus RNA replication
US7659103B2 (en) * 2004-02-20 2010-02-09 Tokyo Metropolitan Organization For Medical Research Nucleic acid construct containing fulllength genome of human hepatitis C virus, recombinant fulllength virus genome-replicating cells having the nucleic acid construct transferred thereinto and method of producing hepatitis C virus Particle

Also Published As

Publication number Publication date
US20090035747A1 (en) 2009-02-05
US8022197B2 (en) 2011-09-20
EP1666598B1 (en) 2015-08-12
CN1882690B (zh) 2011-12-14
WO2005028652A1 (ja) 2005-03-31
CN1882690A (zh) 2006-12-20
JP4573776B2 (ja) 2010-11-04
EP1666598A4 (en) 2006-10-11
JPWO2005028652A1 (ja) 2006-11-30
CA2539457A1 (en) 2005-03-31
EP1666598A1 (en) 2006-06-07

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Effective date: 20200916