CA2532581C - Pre-cast electrophoresis slab gels from supplemented monomer solutions - Google Patents
Pre-cast electrophoresis slab gels from supplemented monomer solutions Download PDFInfo
- Publication number
- CA2532581C CA2532581C CA2532581A CA2532581A CA2532581C CA 2532581 C CA2532581 C CA 2532581C CA 2532581 A CA2532581 A CA 2532581A CA 2532581 A CA2532581 A CA 2532581A CA 2532581 C CA2532581 C CA 2532581C
- Authority
- CA
- Canada
- Prior art keywords
- gel
- accordance
- poly
- cast
- amphiphilic polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000178 monomer Substances 0.000 title claims abstract description 14
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 12
- 239000000499 gel Substances 0.000 title description 71
- 229920000642 polymer Polymers 0.000 claims abstract description 25
- -1 poly(ethylene terephthalate) Polymers 0.000 claims description 69
- 229920002401 polyacrylamide Polymers 0.000 claims description 27
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 26
- 229920003023 plastic Polymers 0.000 claims description 16
- 239000004033 plastic Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 11
- 239000003431 cross linking reagent Substances 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 229920001223 polyethylene glycol Polymers 0.000 claims description 7
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 4
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 4
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 238000001502 gel electrophoresis Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229920002285 poly(styrene-co-acrylonitrile) Polymers 0.000 claims description 3
- 229920000058 polyacrylate Polymers 0.000 claims description 3
- 229920002239 polyacrylonitrile Polymers 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000000379 polymerizing effect Effects 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 238000005266 casting Methods 0.000 abstract description 3
- 230000037361 pathway Effects 0.000 abstract description 3
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- 238000000926 separation method Methods 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical group C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 6
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 4
- 239000011544 gradient gel Substances 0.000 description 4
- 229920001328 Polyvinylidene chloride Polymers 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 229920001824 Barex® Polymers 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 239000006172 buffering agent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003999 initiator Substances 0.000 description 2
- 238000001155 isoelectric focusing Methods 0.000 description 2
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 229920000638 styrene acrylonitrile Polymers 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- OHRWHIOLZGLRKH-UHFFFAOYSA-M [Na+].NCC(O)=O.NCC(O)=O.NCC(O)=O.CCCCCCCCCCCCOS([O-])(=O)=O Chemical compound [Na+].NCC(O)=O.NCC(O)=O.NCC(O)=O.CCCCCCCCCCCCOS([O-])(=O)=O OHRWHIOLZGLRKH-UHFFFAOYSA-M 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000005033 polyvinylidene chloride Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- SCUZVMOVTVSBLE-UHFFFAOYSA-N prop-2-enenitrile;styrene Chemical compound C=CC#N.C=CC1=CC=CC=C1 SCUZVMOVTVSBLE-UHFFFAOYSA-N 0.000 description 1
- 238000007348 radical reaction Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/623,480 | 2003-07-18 | ||
| US10/623,480 US7056426B2 (en) | 2003-01-17 | 2003-07-18 | Pre-cast electrophoresis slab gels with extended storage life |
| PCT/US2004/022790 WO2005010520A1 (en) | 2003-07-18 | 2004-07-14 | Pre-cast electrophoresis slab gels from supplemented monomer solutions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2532581A1 CA2532581A1 (en) | 2005-02-03 |
| CA2532581C true CA2532581C (en) | 2010-04-27 |
Family
ID=34103208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2532581A Expired - Fee Related CA2532581C (en) | 2003-07-18 | 2004-07-14 | Pre-cast electrophoresis slab gels from supplemented monomer solutions |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7056426B2 (https=) |
| EP (1) | EP1649273A4 (https=) |
| JP (1) | JP2006528354A (https=) |
| AU (1) | AU2004260066B2 (https=) |
| CA (1) | CA2532581C (https=) |
| WO (1) | WO2005010520A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0810445D0 (en) | 2008-06-06 | 2008-07-09 | Lab901 Ltd | An electrophoresis cassette |
| JP5333140B2 (ja) * | 2009-10-07 | 2013-11-06 | 凸版印刷株式会社 | 電気泳動ゲル分離媒体 |
| CA3077855C (en) | 2011-01-31 | 2023-01-03 | Biotype Gmbh | Reduced artifact denaturing capillary electrophoresis of nucleic acids |
| JPWO2012133377A1 (ja) * | 2011-03-29 | 2014-07-28 | シャープ株式会社 | 溶液吐出方法、電気泳動用反応器具 |
| WO2013173685A1 (en) | 2012-05-18 | 2013-11-21 | Bio-Rad Laboratories, Inc. | Gelc-ms using stain free technology |
| EP2882524B1 (en) | 2012-08-07 | 2019-05-15 | Bio-Rad Laboratories, Inc. | Method for stain-free protein detection in electrophoresis using modified electrode buffers |
| US9164058B2 (en) | 2013-03-15 | 2015-10-20 | Bio-Rad Laboratories, Inc. | Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability |
| WO2015021418A1 (en) | 2013-08-09 | 2015-02-12 | Bio-Rad Laboratories, Inc. | Protein detection using modified cyclodextrins |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4209373A (en) * | 1979-06-01 | 1980-06-24 | Corning Glass Works | Acidic agar gel electrochromatography of hemoglobins |
| US4314897A (en) * | 1980-07-01 | 1982-02-09 | Beckman Instruments, Inc. | Electrophoretic gel container |
| JPS59126236A (ja) * | 1983-01-08 | 1984-07-20 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| JPS59212751A (ja) * | 1983-05-19 | 1984-12-01 | Fuji Photo Film Co Ltd | 電気泳動媒体材料の製造方法 |
| JPS59212750A (ja) * | 1983-05-19 | 1984-12-01 | Fuji Photo Film Co Ltd | 電気泳動用媒体材料 |
| JPS6060549A (ja) * | 1983-09-14 | 1985-04-08 | Fuji Photo Film Co Ltd | ゲル電気泳動用媒体 |
| JPS6060548A (ja) * | 1983-09-14 | 1985-04-08 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| EP0155833A3 (en) * | 1984-03-15 | 1988-07-27 | Fuji Photo Film Co., Ltd. | Element for electrophoresis |
| JPS60194348A (ja) * | 1984-03-15 | 1985-10-02 | Fuji Photo Film Co Ltd | 電気泳動用媒体材料 |
| DE3582750D1 (de) * | 1984-06-14 | 1991-06-13 | Fuji Photo Film Co Ltd | Elektrophoresemedium. |
| US4806434A (en) * | 1984-07-06 | 1989-02-21 | Fuji Photo Film Co., Ltd. | Medium for electrophoresis |
| JPS62232553A (ja) * | 1986-04-02 | 1987-10-13 | Fuji Photo Film Co Ltd | 電気泳動装置 |
| JPH0660887B2 (ja) * | 1987-09-14 | 1994-08-10 | 富士写真フイルム株式会社 | 電気泳動方法 |
| JP2588059B2 (ja) * | 1990-11-19 | 1997-03-05 | ハイモ株式会社 | 電気泳動用ポリアクリルアミドゲルの製造方法 |
| US5716508A (en) * | 1993-09-09 | 1998-02-10 | Glyko Incorporated | Utilization of additives and defined storage systems to increase the stability and performance of electrophoresis media |
| EP0783691B1 (en) * | 1994-09-19 | 2004-12-01 | Invitrogen Corporation | Plastic mold for electrophoresis gel |
| US5837288A (en) * | 1996-01-11 | 1998-11-17 | Stratagene | Methods for storage of sequencing gels and stored sequencing gels used by such methods |
| US5938906A (en) * | 1997-04-04 | 1999-08-17 | C.C. Imex | Horizontal gel electrophoresis casting cassette |
| JP3942001B2 (ja) * | 1999-12-02 | 2007-07-11 | ハイモ株式会社 | 電気泳動用ポリアクリルアミドプレキャストゲル，その製造方法及び蛋白質の分離分析方法 |
| JP2002277438A (ja) * | 2001-03-22 | 2002-09-25 | Hymo Corp | 電気泳動ゲル、その製法及びその使用法 |
-
2003
- 2003-07-18 US US10/623,480 patent/US7056426B2/en not_active Expired - Lifetime
-
2004
- 2004-07-14 AU AU2004260066A patent/AU2004260066B2/en not_active Ceased
- 2004-07-14 JP JP2006521128A patent/JP2006528354A/ja active Pending
- 2004-07-14 WO PCT/US2004/022790 patent/WO2005010520A1/en not_active Ceased
- 2004-07-14 EP EP04757035A patent/EP1649273A4/en not_active Withdrawn
- 2004-07-14 CA CA2532581A patent/CA2532581C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005010520A1 (en) | 2005-02-03 |
| EP1649273A1 (en) | 2006-04-26 |
| EP1649273A4 (en) | 2011-03-09 |
| AU2004260066B2 (en) | 2010-02-04 |
| CA2532581A1 (en) | 2005-02-03 |
| JP2006528354A (ja) | 2006-12-14 |
| US20040140214A1 (en) | 2004-07-22 |
| US7056426B2 (en) | 2006-06-06 |
| AU2004260066A1 (en) | 2005-02-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20180716 |