CA2489475C - A method for simultaneous production of multiple proteins; vectors and cells for use therein - Google Patents

A method for simultaneous production of multiple proteins; vectors and cells for use therein Download PDF

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Publication number
CA2489475C
CA2489475C CA2489475A CA2489475A CA2489475C CA 2489475 C CA2489475 C CA 2489475C CA 2489475 A CA2489475 A CA 2489475A CA 2489475 A CA2489475 A CA 2489475A CA 2489475 C CA2489475 C CA 2489475C
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CA
Canada
Prior art keywords
sequence
star
gene
polypeptide
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2489475A
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English (en)
French (fr)
Other versions
CA2489475A1 (en
Inventor
Arie Pieter Otte
Arthur Leo Kruckeberg
Richard George Antonius Bernardus Sewalt
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Chromagenics BV
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Chromagenics BV
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Filing date
Publication date
Application filed by Chromagenics BV filed Critical Chromagenics BV
Priority to CA2723500A priority Critical patent/CA2723500C/en
Priority to CA2722998A priority patent/CA2722998C/en
Publication of CA2489475A1 publication Critical patent/CA2489475A1/en
Application granted granted Critical
Publication of CA2489475C publication Critical patent/CA2489475C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2489475A 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein Expired - Lifetime CA2489475C (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2723500A CA2723500C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
CA2722998A CA2722998C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02077350 2002-06-14
EP02077350.3 2002-06-14
PCT/NL2003/000432 WO2003106684A2 (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CA2722998A Division CA2722998C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
CA2723500A Division CA2723500C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Publications (2)

Publication Number Publication Date
CA2489475A1 CA2489475A1 (en) 2003-12-24
CA2489475C true CA2489475C (en) 2011-03-29

Family

ID=29724494

Family Applications (3)

Application Number Title Priority Date Filing Date
CA2489475A Expired - Lifetime CA2489475C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
CA2723500A Expired - Lifetime CA2723500C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
CA2722998A Expired - Lifetime CA2722998C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Family Applications After (2)

Application Number Title Priority Date Filing Date
CA2723500A Expired - Lifetime CA2723500C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
CA2722998A Expired - Lifetime CA2722998C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Country Status (11)

Country Link
US (3) US7364878B2 (enExample)
EP (1) EP1513937B1 (enExample)
JP (3) JP4647309B2 (enExample)
AT (1) ATE500331T1 (enExample)
AU (1) AU2003238719B2 (enExample)
CA (3) CA2489475C (enExample)
DE (1) DE60336228D1 (enExample)
DK (1) DK1513937T3 (enExample)
ES (1) ES2362273T3 (enExample)
NZ (1) NZ537073A (enExample)
WO (1) WO2003106684A2 (enExample)

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ATE421589T1 (de) * 2000-03-17 2009-02-15 Anticancer Inc Optisches abbildungssystem zur darstellung der genexpression des gesamten körpers und anwendungen
EP1806407B1 (en) 2001-07-04 2010-05-05 Chromagenics B.V. DNA sequences having anti-repressor activity
DK1513937T3 (da) 2002-06-14 2011-05-16 Chromagenics Bv Fremgangsmåde til den samtidige produktion af multiple proteiner; vektorer og celler til anvendelse derved
EP1513936A2 (en) * 2002-06-14 2005-03-16 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
EP2314629B2 (en) 2002-07-18 2022-11-16 Merus N.V. Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
CA2510179C (en) * 2002-12-18 2013-04-23 Chromagenics B.V. A method for improving protein production
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
AU2005205412B2 (en) 2004-01-20 2011-08-11 Merus N.V. Mixtures of binding proteins
WO2006005718A2 (en) * 2004-07-08 2006-01-19 Chromagenics B.V. Novel sequence for improving expression of nucleic acid
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
PL1809750T3 (pl) * 2004-11-08 2012-08-31 Chromagenics Bv Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
CN101090968B (zh) 2004-11-08 2011-10-26 科罗迈吉尼科斯公司 高水平表达蛋白质的宿主细胞的选择
US20070042397A1 (en) * 2005-03-03 2007-02-22 International Business Machines Corporation Techniques for linking non-coding and gene-coding deoxyribonucleic acid sequences and applications thereof
CA2637271C (en) * 2006-02-21 2014-12-09 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20080293086A1 (en) * 2006-09-18 2008-11-27 Cobalt Technologies, Inc. A Delaware Corporation Real time monitoring of microbial enzymatic pathways
JP2010504507A (ja) * 2006-09-20 2010-02-12 インスティチュート・パスツール・コリア 細胞におけるターゲットタンパク質候補の発現を検出および／または定量する方法、ならびに小分子モデュレーターのターゲットタンパク質を同定する方法
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
CL2009000879A1 (es) 2008-04-09 2010-05-07 Talfryn S A Metodo para fabricar un producto en un biorreactor que comprende cultivar microorganismos que son adaptados o mutagenizados para que exhiban por lo menos un 150% de tolerancia al producto; sistema para fabricar un producto; biorreactor.
EP2443239B1 (en) 2009-06-15 2015-11-25 Cellagenics B.V. Novel stringent selectable markers
CN102482690A (zh) 2009-06-26 2012-05-30 钴技术有限公司 生物产物生产的集成系统和方法
US9790517B2 (en) 2010-06-15 2017-10-17 Cellagenics B.V. Intergenic elements for enhancing gene expression
US9650630B2 (en) 2010-09-01 2017-05-16 Cellagenics B.V. Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
WO2012040793A1 (en) * 2010-10-01 2012-04-05 Csl Limited Method of cloning nucleic acid
US8945876B2 (en) 2011-11-23 2015-02-03 University Of Hawaii Auto-processing domains for polypeptide expression
DK2838918T3 (da) 2012-04-20 2019-08-12 Merus Nv Fremgangsmåder og midler til fremstilling af heterodimere ig-lignende molekyler
WO2015053523A1 (ko) * 2013-10-07 2015-04-16 한화케미칼 주식회사 항체 발현용 바이시스트로닉 발현벡터 및 이를 이용한 항체의 생산 방법
EP4569107A1 (en) * 2022-08-09 2025-06-18 GEG Tech Transient expression system for rna, for gene editing
US20260041746A1 (en) * 2022-08-09 2026-02-12 Geg Tech Transient expression system for rna, for vaccination
WO2024033444A1 (en) * 2022-08-09 2024-02-15 Geg Tech Transient expression system for rna, for cosmetic uses
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EP1513936A2 (en) * 2002-06-14 2005-03-16 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
DK1513937T3 (da) 2002-06-14 2011-05-16 Chromagenics Bv Fremgangsmåde til den samtidige produktion af multiple proteiner; vektorer og celler til anvendelse derved
CA2510179C (en) 2002-12-18 2013-04-23 Chromagenics B.V. A method for improving protein production
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
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Also Published As

Publication number Publication date
US7901908B2 (en) 2011-03-08
US20050191723A1 (en) 2005-09-01
WO2003106684A3 (en) 2004-04-08
ES2362273T3 (es) 2011-06-30
US7960143B2 (en) 2011-06-14
JP5095693B2 (ja) 2012-12-12
CA2723500A1 (en) 2003-12-24
JP5095690B2 (ja) 2012-12-12
US20080227199A1 (en) 2008-09-18
US7364878B2 (en) 2008-04-29
JP2010004886A (ja) 2010-01-14
CA2722998A1 (en) 2003-12-24
CA2722998C (en) 2014-12-02
US20080227151A1 (en) 2008-09-18
NZ537073A (en) 2006-08-31
WO2003106684A2 (en) 2003-12-24
AU2003238719A1 (en) 2003-12-31
JP2010011859A (ja) 2010-01-21
CA2723500C (en) 2014-10-28
ATE500331T1 (de) 2011-03-15
DE60336228D1 (de) 2011-04-14
EP1513937A2 (en) 2005-03-16
JP2005529610A (ja) 2005-10-06
JP4647309B2 (ja) 2011-03-09
AU2003238719B2 (en) 2008-02-21
DK1513937T3 (da) 2011-05-16
CA2489475A1 (en) 2003-12-24
EP1513937B1 (en) 2011-03-02

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Class et al. Patent application title: Method for simultaneous production of multiple proteins; vectors and cells for use therein Inventors: Arie Pieter Otte (Amersfoort, NL) Arie Pieter Otte (Amersfoort, NL) Arthur Leo Kruckeberg (Shoreline, WA, US) Richard George Antonius Bernardus Sewalt (Arnhem, NL) Assignees: Crucell Holland BV
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