CA2487135C - Procedes de fragmentation pour spectrometrie de masse - Google Patents
Procedes de fragmentation pour spectrometrie de masse Download PDFInfo
- Publication number
- CA2487135C CA2487135C CA002487135A CA2487135A CA2487135C CA 2487135 C CA2487135 C CA 2487135C CA 002487135 A CA002487135 A CA 002487135A CA 2487135 A CA2487135 A CA 2487135A CA 2487135 C CA2487135 C CA 2487135C
- Authority
- CA
- Canada
- Prior art keywords
- low
- aperture
- energy electrons
- electrons
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000013467 fragmentation Methods 0.000 title abstract description 40
- 238000006062 fragmentation reaction Methods 0.000 title abstract description 40
- 238000000034 method Methods 0.000 title abstract description 37
- 238000004949 mass spectrometry Methods 0.000 title description 4
- 150000002500 ions Chemical class 0.000 claims abstract description 334
- 230000005405 multipole Effects 0.000 claims abstract description 174
- 238000010894 electron beam technology Methods 0.000 claims description 32
- 230000005684 electric field Effects 0.000 claims description 26
- 239000000126 substance Substances 0.000 claims description 6
- 239000002243 precursor Substances 0.000 abstract description 48
- 238000006243 chemical reaction Methods 0.000 abstract description 12
- 230000005264 electron capture Effects 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 description 149
- 238000001211 electron capture detection Methods 0.000 description 47
- 239000007789 gas Substances 0.000 description 40
- 238000001360 collision-induced dissociation Methods 0.000 description 35
- 238000002347 injection Methods 0.000 description 17
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- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 3
- 229910052721 tungsten Inorganic materials 0.000 description 3
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
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- -1 with mass > 20 Chemical class 0.000 description 2
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
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- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
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- 206010003549 asthenia Diseases 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
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- 238000007906 compression Methods 0.000 description 1
- 239000000112 cooling gas Substances 0.000 description 1
- PMHQVHHXPFUNSP-UHFFFAOYSA-M copper(1+);methylsulfanylmethane;bromide Chemical compound Br[Cu].CSC PMHQVHHXPFUNSP-UHFFFAOYSA-M 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000002019 doping agent Substances 0.000 description 1
- 238000001803 electron scattering Methods 0.000 description 1
- 230000005442 electron-positron pair Effects 0.000 description 1
- 238000002212 electronic circular dichroism spectrum Methods 0.000 description 1
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- 230000000670 limiting effect Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000004150 penning trap Methods 0.000 description 1
- 230000010363 phase shift Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920003223 poly(pyromellitimide-1,4-diphenyl ether) Polymers 0.000 description 1
- 238000005036 potential barrier Methods 0.000 description 1
- 238000005381 potential energy Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
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- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 1
- 229910010271 silicon carbide Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/06—Electron- or ion-optical arrangements
- H01J49/062—Ion guides
- H01J49/063—Multipole ion guides, e.g. quadrupoles, hexapoles
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
- H01J49/0045—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction
- H01J49/0054—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction by an electron beam, e.g. electron impact dissociation, electron capture dissociation
Abstract
L'invention concerne un appareil et des procédés qui permettent l'interaction d'électrons à faible énergie et de positrons ayant des ions d'échantillonnage qui facilitent la dissociation à capture d'électrons (ECD) et la dissociation à capture de positrons (PCD), respectivement, dans les structures de guidage d'ions multipôle. Il a été découvert récemment que la fragmentation d'ions protonés de plusieurs biomolécules par dissociation à capture d'électrons se produit le long de chemins de fragmentation auxquels d'autres procédés de dissociation n'ont pas accès. Ces chemins mènent à des informations de structure moléculaire qui ne sont pas si faciles à obtenir autrement. Cependant, de telles analyses ont été limitées aux spectromètres de masse à résonance cyclotronique ionique à haut champ et transformée de Fourrier (FTICR) onéreux. La mise en oeuvre de la dissociation de capture d'électrons (ECD) dans des structures de guidage d'ions multipôles utilisées couramment est problématique dû aux effets perturbateurs de champs RF dans ces dispositifs. L'appareil et les procédés décrits surmontent ces difficultés, et permettent d'exécuter la dissociation à capture d'électrons (et dissociation à capture de positrons) dans les guides d'ions multipôles, soit tout seuls, soit combinés aux procédés de fragmentation d'ions conventionnels. L'invention concerne donc l'exécution analytique améliorée et la fonctionnalité de spectromètres de masse qui utilisent des guides d'ions multipôles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2643534A CA2643534C (fr) | 2002-05-31 | 2003-05-30 | Procedes de fragmentation pour spectrometrie de masse |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38511302P | 2002-05-31 | 2002-05-31 | |
| US60/385,113 | 2002-05-31 | ||
| PCT/US2003/017436 WO2003102545A2 (fr) | 2002-05-31 | 2003-05-30 | Procedes de fragmentation pour spectrometrie de masse |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2643534A Division CA2643534C (fr) | 2002-05-31 | 2003-05-30 | Procedes de fragmentation pour spectrometrie de masse |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2487135A1 CA2487135A1 (fr) | 2003-12-11 |
| CA2487135C true CA2487135C (fr) | 2009-01-27 |
Family
ID=29712135
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002487135A Expired - Lifetime CA2487135C (fr) | 2002-05-31 | 2003-05-30 | Procedes de fragmentation pour spectrometrie de masse |
| CA2643534A Expired - Lifetime CA2643534C (fr) | 2002-05-31 | 2003-05-30 | Procedes de fragmentation pour spectrometrie de masse |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2643534A Expired - Lifetime CA2643534C (fr) | 2002-05-31 | 2003-05-30 | Procedes de fragmentation pour spectrometrie de masse |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1549923B1 (fr) |
| AU (1) | AU2003247472A1 (fr) |
| CA (2) | CA2487135C (fr) |
| WO (1) | WO2003102545A2 (fr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020115056A1 (en) | 2000-12-26 | 2002-08-22 | Goodlett David R. | Rapid and quantitative proteome analysis and related methods |
| GB0305796D0 (en) | 2002-07-24 | 2003-04-16 | Micromass Ltd | Method of mass spectrometry and a mass spectrometer |
| DE10325582B4 (de) * | 2003-06-05 | 2009-01-15 | Bruker Daltonik Gmbh | Ionenfragmentierung durch Elektroneneinfang in Hochfrequenz-Ionenfallen mit magnetischer Führung der Elektronen |
| DE10325579B4 (de) * | 2003-06-05 | 2007-10-11 | Bruker Daltonik Gmbh | Ionenfragmentierung durch Elektroneneinfang in linearen Ionenfallen |
| US6800851B1 (en) * | 2003-08-20 | 2004-10-05 | Bruker Daltonik Gmbh | Electron-ion fragmentation reactions in multipolar radiofrequency fields |
| US7026613B2 (en) | 2004-01-23 | 2006-04-11 | Thermo Finnigan Llc | Confining positive and negative ions with fast oscillating electric potentials |
| JP4275545B2 (ja) | 2004-02-17 | 2009-06-10 | 株式会社日立ハイテクノロジーズ | 質量分析装置 |
| GB0404106D0 (en) | 2004-02-24 | 2004-03-31 | Shimadzu Res Lab Europe Ltd | An ion trap and a method for dissociating ions in an ion trap |
| WO2005090978A1 (fr) | 2004-03-12 | 2005-09-29 | University Of Virginia Patent Foundation | Dissociation par transfert d'electrons pour analyse de sequence de biopolymeres |
| GB2414855A (en) * | 2004-03-30 | 2005-12-07 | Thermo Finnigan Llc | Ion fragmentation by electron capture |
| DE102004028419B4 (de) | 2004-06-11 | 2011-06-22 | Bruker Daltonik GmbH, 28359 | Massenspektrometer und Reaktionszelle für Ionen-Ionen-Reaktionen |
| GB2415541B (en) * | 2004-06-21 | 2009-09-23 | Thermo Finnigan Llc | RF power supply for a mass spectrometer |
| GB2432712B (en) | 2005-11-23 | 2007-12-27 | Micromass Ltd | Mass spectrometer |
| GB0523806D0 (en) * | 2005-11-23 | 2006-01-04 | Micromass Ltd | Mass spectrometer |
| GB0523811D0 (en) * | 2005-11-23 | 2006-01-04 | Micromass Ltd | Mass stectrometer |
| GB0609253D0 (en) | 2006-05-10 | 2006-06-21 | Micromass Ltd | Mass spectrometer |
| WO2023150866A1 (fr) * | 2022-02-08 | 2023-08-17 | Kimia Analytics Inc. | Cellule de collision à cible solide pour spectrométrie de masse |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4731533A (en) * | 1986-10-15 | 1988-03-15 | Vestec Corporation | Method and apparatus for dissociating ions by electron impact |
| CA1307859C (fr) | 1988-12-12 | 1992-09-22 | Donald James Douglas | Spectrometre de masse a transmission amelioree d'ions |
| US4933551A (en) * | 1989-06-05 | 1990-06-12 | The United State Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Reversal electron attachment ionizer for detection of trace species |
| US4988869A (en) * | 1989-08-21 | 1991-01-29 | The Regents Of The University Of California | Method and apparatus for electron-induced dissociation of molecular species |
| DE10058706C1 (de) * | 2000-11-25 | 2002-02-28 | Bruker Daltonik Gmbh | Ionenfragmentierung durch Elektroneneinfang in Hochfrequenz-Ionenfallen |
| US6958472B2 (en) * | 2001-03-22 | 2005-10-25 | Syddansk Universitet | Mass spectrometry methods using electron capture by ions |
-
2003
- 2003-05-30 CA CA002487135A patent/CA2487135C/fr not_active Expired - Lifetime
- 2003-05-30 WO PCT/US2003/017436 patent/WO2003102545A2/fr not_active Application Discontinuation
- 2003-05-30 EP EP03756376A patent/EP1549923B1/fr not_active Expired - Lifetime
- 2003-05-30 CA CA2643534A patent/CA2643534C/fr not_active Expired - Lifetime
- 2003-05-30 AU AU2003247472A patent/AU2003247472A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2643534C (fr) | 2011-08-02 |
| AU2003247472A8 (en) | 2003-12-19 |
| WO2003102545A3 (fr) | 2004-05-06 |
| CA2487135A1 (fr) | 2003-12-11 |
| EP1549923A4 (fr) | 2009-03-25 |
| EP1549923A2 (fr) | 2005-07-06 |
| EP1549923B1 (fr) | 2012-10-17 |
| CA2643534A1 (fr) | 2003-12-11 |
| AU2003247472A1 (en) | 2003-12-19 |
| WO2003102545A2 (fr) | 2003-12-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20230530 |