CA2484397C - Methods for measuring protein kinase and phosphatase activity - Google Patents

Methods for measuring protein kinase and phosphatase activity Download PDF

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Publication number
CA2484397C
CA2484397C CA2484397A CA2484397A CA2484397C CA 2484397 C CA2484397 C CA 2484397C CA 2484397 A CA2484397 A CA 2484397A CA 2484397 A CA2484397 A CA 2484397A CA 2484397 C CA2484397 C CA 2484397C
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CA
Canada
Prior art keywords
substrate
enzyme
phosphorylated
group
labelled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2484397A
Other languages
English (en)
French (fr)
Other versions
CA2484397A1 (en
Inventor
John Gerard Whateley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SPT Labtech Ltd
Original Assignee
GE Healthcare UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare UK Ltd filed Critical GE Healthcare UK Ltd
Publication of CA2484397A1 publication Critical patent/CA2484397A1/en
Application granted granted Critical
Publication of CA2484397C publication Critical patent/CA2484397C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2484397A 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity Expired - Fee Related CA2484397C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0208987.8 2002-04-19
GBGB0208987.8A GB0208987D0 (en) 2002-04-19 2002-04-19 Methods for measuring protein kinase and phosphatase activity
PCT/GB2003/001683 WO2003089665A1 (en) 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity

Publications (2)

Publication Number Publication Date
CA2484397A1 CA2484397A1 (en) 2003-10-30
CA2484397C true CA2484397C (en) 2014-06-17

Family

ID=9935150

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2484397A Expired - Fee Related CA2484397C (en) 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity

Country Status (10)

Country Link
US (1) US7125682B2 (https=)
EP (1) EP1497453B1 (https=)
JP (1) JP4615867B2 (https=)
AT (1) ATE364715T1 (https=)
AU (1) AU2003227872A1 (https=)
CA (1) CA2484397C (https=)
DE (1) DE60314395T2 (https=)
GB (2) GB0208987D0 (https=)
IL (1) IL164543A0 (https=)
WO (1) WO2003089665A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090118139A1 (en) * 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
DE502004006979D1 (de) * 2003-04-10 2008-06-12 Tecan Trading Ag Verfahren zum Messen von an biologische Moleküle gebundenen, chemischen Gruppen
WO2005043137A1 (de) * 2003-10-18 2005-05-12 Bayer Healthcare Ag Direkte beobachtung molekularer veränderungen in biologischen testsystemen mittels messungen der fluoreszenz-lebensdauer
US20060084128A1 (en) * 2004-09-22 2006-04-20 Hongye Sun Enzyme assay with nanowire sensor
EP1940965B1 (en) * 2005-10-28 2014-03-26 Almac Sciences (Scotland) Limited Novel fluorescent dyes and uses thereof
GB0712109D0 (en) * 2007-06-22 2007-08-01 Edinburgh Instr Fluorescence lifetime and fluorescence assays
FI20070508A0 (fi) * 2007-06-27 2007-06-27 Turun Yliopisto Peptidisubstraatti ja sen käyttö
DE102008011578A1 (de) * 2008-02-28 2009-09-03 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Photolumineszenz-Sensor
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes
CN116981931A (zh) * 2021-03-14 2023-10-31 安捷伦科技有限公司 用于分析生物学材料的系统和方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032381A (en) * 1988-12-20 1991-07-16 Tropix, Inc. Chemiluminescence-based static and flow cytometry
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
JP3815051B2 (ja) 1997-04-30 2006-08-30 和光純薬工業株式会社 新規なペプチド誘導体
DK1036192T3 (da) * 1997-12-05 2003-06-10 Upjohn Co Fluorescensbaserede screeningsanalyser med høj hastighedforproteinkinaser og phosphataser
JP2001019700A (ja) 1999-07-07 2001-01-23 Mitsubishi Chemicals Corp 蛍光標識ペプチド
JP3819294B2 (ja) 1999-07-30 2006-09-06 バイエル コーポレイション 燐酸酵素のような加水分解性酵素の化学発光基質
EP1264897A3 (en) 2001-06-06 2003-11-12 Europäisches Laboratorium Für Molekularbiologie (Embl) Synthetic sensor peptide for kinase or phosphatase assays

Also Published As

Publication number Publication date
US7125682B2 (en) 2006-10-24
WO2003089665A1 (en) 2003-10-30
IL164543A0 (en) 2005-12-18
GB0208987D0 (en) 2002-05-29
CA2484397A1 (en) 2003-10-30
WO2003089665A8 (en) 2004-11-18
GB0308903D0 (en) 2003-05-21
EP1497453A1 (en) 2005-01-19
AU2003227872A1 (en) 2003-11-03
DE60314395T2 (de) 2008-02-21
JP4615867B2 (ja) 2011-01-19
GB2387905A (en) 2003-10-29
GB2387905B (en) 2005-01-12
DE60314395D1 (de) 2007-07-26
ATE364715T1 (de) 2007-07-15
JP2005523024A (ja) 2005-08-04
US20030228646A1 (en) 2003-12-11
EP1497453B1 (en) 2007-06-13

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Legal Events

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EEER Examination request
MKLA Lapsed

Effective date: 20220301

MKLA Lapsed

Effective date: 20200831