CA2483285C - Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same - Google Patents

Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same Download PDF

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Publication number
CA2483285C
CA2483285C CA2483285A CA2483285A CA2483285C CA 2483285 C CA2483285 C CA 2483285C CA 2483285 A CA2483285 A CA 2483285A CA 2483285 A CA2483285 A CA 2483285A CA 2483285 C CA2483285 C CA 2483285C
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CA
Canada
Prior art keywords
framework
antibody
ser
gly
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2483285A
Other languages
English (en)
French (fr)
Other versions
CA2483285A1 (en
Inventor
Kathrin Tissot
Stefan Ewert
Adrian Auf Der Maur
Alcide Barberis
Dominik Escher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Esbatech An Alcon Biomedical Research Unit LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Esbatech An Alcon Biomedical Research Unit LLC filed Critical Esbatech An Alcon Biomedical Research Unit LLC
Priority to CA2867542A priority Critical patent/CA2867542C/en
Publication of CA2483285A1 publication Critical patent/CA2483285A1/en
Application granted granted Critical
Publication of CA2483285C publication Critical patent/CA2483285C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C07K2317/82—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies functional in the cytoplasm, the inner aspect of the cell membrane, the nucleus or the mitochondria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2483285A 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same Expired - Lifetime CA2483285C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2867542A CA2867542C (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38264902P 2002-05-22 2002-05-22
US60/382,649 2002-05-22
US43825603P 2003-01-03 2003-01-03
US60/438,256 2003-01-03
PCT/EP2003/005324 WO2003097697A2 (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2867542A Division CA2867542C (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Publications (2)

Publication Number Publication Date
CA2483285A1 CA2483285A1 (en) 2003-11-27
CA2483285C true CA2483285C (en) 2015-01-27

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CA2483285A Expired - Lifetime CA2483285C (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
CA2867542A Expired - Lifetime CA2867542C (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2867542A Expired - Lifetime CA2867542C (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Country Status (14)

Country Link
US (5) US8853362B2 (enExample)
EP (5) EP2332989B1 (enExample)
JP (10) JP2006508638A (enExample)
CN (4) CN102093477B (enExample)
AU (2) AU2003238370B2 (enExample)
CA (2) CA2483285C (enExample)
CY (1) CY1114225T1 (enExample)
DK (2) DK2332989T3 (enExample)
ES (3) ES2399617T3 (enExample)
HU (1) HUE048922T2 (enExample)
NZ (1) NZ536412A (enExample)
PT (2) PT1506236E (enExample)
SI (2) SI2332989T1 (enExample)
WO (1) WO2003097697A2 (enExample)

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Also Published As

Publication number Publication date
US8853362B2 (en) 2014-10-07
CN103739706B (zh) 2015-11-18
EP2947095B1 (en) 2019-10-02
AU2003238370A1 (en) 2003-12-02
ES2537104T3 (es) 2015-06-02
CN105175535A (zh) 2015-12-23
JP2013078343A (ja) 2013-05-02
ES2399617T3 (es) 2013-04-02
JP2017201990A (ja) 2017-11-16
JP2020171286A (ja) 2020-10-22
HUE048922T2 (hu) 2020-09-28
US10570190B2 (en) 2020-02-25
US20190119361A1 (en) 2019-04-25
US10125186B2 (en) 2018-11-13
JP2015227372A (ja) 2015-12-17
JP2015214583A (ja) 2015-12-03
JP2013256533A (ja) 2013-12-26
PT2332989E (pt) 2015-08-05
JP2010187678A (ja) 2010-09-02
HK1071378A1 (en) 2005-07-15
JP2019058191A (ja) 2019-04-18
EP2332989B1 (en) 2015-04-01
US9518108B2 (en) 2016-12-13
DK2332989T3 (en) 2015-05-11
EP2332989A1 (en) 2011-06-15
EP1506236A2 (en) 2005-02-16
JP2012228272A (ja) 2012-11-22
US20150057191A1 (en) 2015-02-26
CA2483285A1 (en) 2003-11-27
EP1506236B1 (en) 2013-01-23
EP2314622A1 (en) 2011-04-27
CN1662556A (zh) 2005-08-31
US20170107272A1 (en) 2017-04-20
WO2003097697A3 (en) 2004-12-09
HK1217712A1 (en) 2017-01-20
CA2867542C (en) 2020-04-14
EP2314622B1 (en) 2017-10-18
EP3656787A1 (en) 2020-05-27
CN102093477A (zh) 2011-06-15
JP6261136B2 (ja) 2018-01-17
AU2010202634B2 (en) 2012-08-23
HK1152320A1 (en) 2012-02-24
US20200165322A1 (en) 2020-05-28
JP2006508638A (ja) 2006-03-16
PT1506236E (pt) 2013-03-27
CA2867542A1 (en) 2003-11-27
EP2947095A1 (en) 2015-11-25
NZ536412A (en) 2008-10-31
CN103739706A (zh) 2014-04-23
SI2332989T1 (sl) 2015-06-30
DK1506236T3 (da) 2013-03-04
JP6918033B2 (ja) 2021-08-11
JP6075836B2 (ja) 2017-02-08
CN102093477B (zh) 2014-02-26
WO2003097697A2 (en) 2003-11-27
US20060035320A1 (en) 2006-02-16
JP6100544B2 (ja) 2017-03-22
AU2010202634A1 (en) 2010-07-15
CY1114225T1 (el) 2016-08-31
SI2947095T1 (sl) 2020-04-30
AU2003238370B2 (en) 2010-04-29
CN1662556B (zh) 2013-05-01
ES2656427T3 (es) 2018-02-27

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